Restriction map for
pFN21AE0130
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DraIII EagI EarI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI Ksp632I MamI
MboII MfeI MflI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcsI 100 GAATTC [GA]^AATT[CT]
AcyI 370 GGCGCC G[GA]^CG[CT]C
AhdI 700 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 625 GTGCAC G^TGCAC
AlwI 410 GATCC GGATC(4/5)
AlwNI 457 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 625 GTGCAC G^TGCAC
ApoI 100 GAATTC [GA]^AATT[CT]
AspEI 700 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 389 TGCGCA TGC^GCA
BalI 827 TGGCCA TGG^CCA
BbeI 370 GGCGCC GGCGC^C
BbvCI 1144 GCTGAGG CCTCAGC(-5/-2)
BlpI 742 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 742 GCTCAGC GC^T[ACGT]AGC
BsaHI 370 GGCGCC G[GA]^CG[CT]C
BsmBI 763 CGTCTC CGTCTC(1/5)
BsrBI 740 CCGCTC CCGCTC(-3/-3)
BssSI 622 CTCGTG CACGAG(-5/-1)
BstEII 6 GGTGACC G^GT[ACGT]ACC
BstPI 6 GGTGACC G^GT[ACGT]ACC
BstXI 687 CCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 742 GCTCAGC GC^T[ACGT]AGC
DpnII 410 GATC ^GATC
DrdI 208 GACCGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 700 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 6 GGTGACC G^GT[ACGT]ACC
EcoRI 100 GAATTC G^AATTC
EcoT14I 809 CCATGG C^C[AT][AT]GG
FauI 649 CCCGC CCCGC(4/6)
FspI 389 TGCGCA TGC^GCA
HaeII 370 GGCGCC [GA]GCGC^[CT]
HgaI 762 GCGTC GACGC(5/10)
Hin1I 370 GGCGCC G[GA]^CG[CT]C
KasI 370 GGCGCC G^GCGCC
KspI 188 CCGCGG CCGC^GG
MaeII 269 ACGT A^CGT
MaeIII 7 GTGAC ^GT[ACGT]AC
MboI 410 GATC ^GATC
MluNI 827 TGGCCA TGG^CCA
MscI 827 TGGCCA TGG^CCA
MspA1I 188 CCGCGG C[AC]G^C[GT]G
NaeI 367 GCCGGC GCC^GGC
NarI 370 GGCGCC GG^CGCC
NcoI 809 CCATGG C^CATGG
NdeII 410 GATC ^GATC
NgoMIV 367 GCCGGC G^CCGGC
NspI 546 GCATGT [GA]CATG^[CT]
PaeR7I 115 CTCGAG C^TCGAG
PflMI 456 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1095 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 188 CCGCGG CCGC^GG
Sau3AI 410 GATC ^GATC
SfoI 370 GGCGCC GGC^GCC
SmaI 1037 CCCGGG CCC^GGG
StuI 130 AGGCCT AGG^CCT
StyI 809 CCATGG C^C[AT][AT]GG
Tsp45I 7 GTGAC ^GT[GC]AC
Tsp509I 101 AATT ^AATT
Van91I 456 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 185 CCACCGCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 115 CTCGAG C^TCGAG
XmaCI 1037 CCCGGG C^CCGGG
XmaI 1037 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AocI 126 CCTGAGG CC^T[ACGT]AGG
855 CCTGAGG
ApaI 258 GGGCCC GGGCC^C
875 GGGCCC
AvaI 115 CTCGAG C^[CT]CG[GA]G
1037 CCCGGG
BglI 184 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
652 GCCAGGACGGC
BsoBI 115 CTCGAG C^[CT]CG[GA]G
1037 CCCGGG
BstAPI 392 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
618 GCAGCTCGTGC
Bsu36I 126 CCTGAGG CC^T[ACGT]AGG
855 CCTGAGG
Eco81I 126 CCTGAGG CC^T[ACGT]AGG
855 CCTGAGG
HincII 43 GTCAAC GT[CT]^[GA]AC
217 GTCAAC
HindII 43 GTCAAC GT[CT]^[GA]AC
217 GTCAAC
MslI 534 CACCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
720 CATCCGGGTG
SacI 202 GAGCTC GAGCT^C
1150 GAGCTC
SmlI 115 CTCGAG C^T[CT][GA]AG
1168 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoNI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MnlI MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I