(Circular) (Six-base) MAPSORT of: pFN21AE0130.seq Check: 4014 from: 1 to: 5902 pFN21AE0130 5902 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 3201 4029 4402 1563 Size: 1638 828 373 3063 Fragments arranged by size: 3063 1638 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3965 5349 5617 1632 Size: 2333 1384 268 1917 Fragments arranged by size: 2333 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 2710 4631 2710 Size: 1921 3981 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2081 1184 Size: 897 5005 AlwNI CAG_nnn'CTG Cuts at: 2467 3645 5217 2467 Size: 1178 1572 3152 Fragments arranged by size: 3152 1572 1178 ApaI G_GGCC'C Cuts at: 2267 2884 2267 Size: 617 5285 ApaLI G'TGCA_C Cuts at: 2630 3959 5115 2630 Size: 1329 1156 3417 Fragments arranged by size: 3417 1329 1156 ApoI r'AATT_y Cuts at: 1493 2105 3211 3437 3589 5698 1493 Size: 612 1106 226 152 2109 1697 Fragments arranged by size: 2109 1697 1106 612 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2120 3042 3227 1870 Size: 73 177 922 185 4545 Fragments arranged by size: 4545 922 185 177 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2375 2457 3038 3050 Size: 325 217 627 244 344 82 581 12 Cuts at: 3050 3223 4678 618 Size: 173 1455 1842 Fragments arranged by size: 1842 1455 627 581 344 325 244 217 173 82 12 BanII G_rGCy'C Cuts at: 1391 2211 2267 2884 2967 3019 3159 3174 3221 Size: 820 56 617 83 52 140 15 47 Cuts at: 3221 1391 Size: 4072 Fragments arranged by size: 4072 820 617 140 83 56 52 47 15 BbeI G_GCGC'C Cuts at: 2379 2379 Size: 5902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BbvCI CC'TCA_GC Cuts at: 3150 3150 Size: 5902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2195 2663 4513 Size: 107 122 71 631 605 523 468 1850 Cuts at: 4513 136 Size: 1525 Fragments arranged by size: 1850 1525 631 605 523 468 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 2748 3319 2748 Size: 571 5331 Bme1580I G_kGCm'C Cuts at: 1790 2267 2289 2634 2884 3043 3055 3963 5119 Size: 477 22 345 250 159 12 908 1156 Cuts at: 5119 1790 Size: 2573 Fragments arranged by size: 2573 1156 908 477 345 250 159 22 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2268 3633 4591 Size: 416 53 83 399 24 851 1365 958 Cuts at: 4591 442 Size: 1753 Fragments arranged by size: 1753 1365 958 851 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3113 3146 3206 4562 1239 Size: 1874 33 60 1356 2579 Fragments arranged by size: 2579 1874 1356 60 33 Bpu10I CC'TnA_GC Cuts at: 2296 3150 2296 Size: 854 5048 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3158 4027 4892 5190 3158 Size: 869 865 298 3870 Fragments arranged by size: 3870 869 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 2572 2943 4565 882 Size: 617 822 251 371 1622 2219 Fragments arranged by size: 2219 1622 822 617 371 251 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2376 3709 4091 4726 Size: 53 83 186 936 843 1333 382 635 Cuts at: 4726 275 Size: 1451 Fragments arranged by size: 1451 1333 936 843 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2318 2522 2596 2599 2602 3116 3149 Size: 1006 126 204 74 3 3 514 33 Cuts at: 3149 3167 1186 Size: 18 3921 Fragments arranged by size: 3921 1006 514 204 126 74 33 18 3 3 BseYI C'CCAG_C Cuts at: 2959 5105 5601 2959 Size: 2146 496 3260 Fragments arranged by size: 3260 2146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2215 2323 2851 3352 4113 5141 Size: 275 186 214 108 528 501 761 1028 Cuts at: 5141 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 528 501 275 214 186 108 BsiHKAI G_wGCw'C Cuts at: 2211 2634 3159 3963 4048 5119 2211 Size: 423 525 804 85 1071 2994 Fragments arranged by size: 2994 1071 804 525 423 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 BsmBI CGTCTCn'nnnn_ Cuts at: 2774 2774 Size: 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2267 2289 2634 2884 2967 3019 Size: 399 421 56 22 345 250 83 52 Cuts at: 3019 3043 3055 3159 3174 3221 3963 4048 5119 Size: 24 12 104 15 47 742 85 1071 Cuts at: 5119 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 421 399 345 250 104 85 83 56 52 47 24 22 15 12 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3257 844 Size: 2413 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2747 3789 1394 Size: 429 924 1042 3507 Fragments arranged by size: 3507 1042 924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 2372 3035 4546 4733 1700 Size: 110 224 338 663 1511 187 2869 Fragments arranged by size: 2869 1511 663 338 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 2627 3962 4974 2627 Size: 1335 1012 3555 Fragments arranged by size: 3555 1335 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2403 2629 794 Size: 1609 226 4067 Fragments arranged by size: 4067 1609 226 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 2011 1184 Size: 827 5075 BstXI CCAn_nnnn'nTGG Cuts at: 2699 2699 Size: 5902 BstYI r'GATC_y Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897 Size: 1260 367 385 17 1452 86 12 346 Cuts at: 5897 1972 Size: 1977 Fragments arranged by size: 1977 1452 1260 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2132 2861 2132 Size: 729 5173 BtgI C'CryG_G Cuts at: 513 1065 2004 2193 2267 2814 513 Size: 552 939 189 74 547 3601 Fragments arranged by size: 3601 939 552 547 189 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 3418 4212 4232 3418 Size: 794 20 5088 Fragments arranged by size: 5088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2219 4909 809 Size: 507 903 2690 1802 Fragments arranged by size: 2690 1802 903 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2832 2928 Size: 54 1425 50 275 18 540 462 96 Cuts at: 2928 3349 4238 5867 8 Size: 421 889 1629 43 Fragments arranged by size: 1629 1425 889 540 462 421 275 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3832 1353 Size: 2479 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2660 2833 4489 4863 5009 387 Size: 1064 138 1071 173 1656 374 146 1280 Fragments arranged by size: 1656 1280 1071 1064 374 173 146 138 EcoICRI GAG'CTC Cuts at: 2209 3157 2209 Size: 948 4954 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3113 3146 3206 3965 4562 5349 5617 Size: 393 1481 33 60 759 597 787 268 Cuts at: 5617 1239 Size: 1524 Fragments arranged by size: 1524 1481 787 759 597 393 268 60 33 EcoNI CCTnn'n_nnAGG Cuts at: 2690 3128 3164 2690 Size: 438 36 5428 Fragments arranged by size: 5428 438 36 EcoO109I rG'GnC_Cy Cuts at: 2062 2263 2827 2880 3101 3121 2062 Size: 201 564 53 221 20 4843 Fragments arranged by size: 4843 564 221 201 53 20 EcoRI G'AATT_C Cuts at: 2105 3211 3589 2105 Size: 1106 378 4418 Fragments arranged by size: 4418 1106 378 FspI TGC'GCA Cuts at: 2396 4408 2396 Size: 2012 3890 HaeII r_GCGC'y Cuts at: 1678 2379 5049 1678 Size: 701 2670 2531 Fragments arranged by size: 2670 2531 701 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2696 2728 4543 4575 4617 Size: 32 135 32 1124 32 1815 32 42 Cuts at: 4617 4649 1373 Size: 32 2626 Fragments arranged by size: 2626 1815 1124 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2050 2224 3246 3492 669 Size: 988 281 112 174 1022 246 3079 Fragments arranged by size: 3079 1022 988 281 246 174 112 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KasI G'GCGC_C Cuts at: 2375 2375 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2834 10 Size: 54 1425 343 1002 3078 Fragments arranged by size: 3078 1425 1002 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2543 2729 3860 4219 4378 518 Size: 648 843 534 186 1131 359 159 2042 Fragments arranged by size: 2042 1131 843 648 534 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2195 3318 3995 5143 5388 1606 Size: 589 1123 677 1148 245 2120 Fragments arranged by size: 2120 1148 1123 677 589 245 NaeI GCC'GGC Cuts at: 2374 2374 Size: 5902 NarI GG'CG_CC Cuts at: 2376 2376 Size: 5902 NcoI C'CATG_G Cuts at: 513 1065 2004 2814 513 Size: 552 939 810 3601 Fragments arranged by size: 3601 939 810 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NgoMIV G'CCGG_C Cuts at: 2372 2372 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2555 3260 4805 1132 Size: 97 1326 705 1545 2229 Fragments arranged by size: 2229 1545 1326 705 97 PasI CC'CwG_GG Cuts at: 1348 2241 2380 2439 2501 1348 Size: 893 139 59 62 4749 Fragments arranged by size: 4749 893 139 62 59 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2467 1108 Size: 1359 4543 PfoI T'CCnGG_A Cuts at: 2082 2337 2480 3162 3183 2082 Size: 255 143 682 21 4801 Fragments arranged by size: 4801 682 255 143 21 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3101 3101 Size: 5902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PspOMI G'GGCC_C Cuts at: 2263 2880 2263 Size: 617 5285 PspXI vC'TCGA_Gb Cuts at: 2120 2120 Size: 5902 PstI C_TGCA'G Cuts at: 830 2025 2625 2808 3254 830 Size: 1195 600 183 446 3478 Fragments arranged by size: 3478 1195 600 446 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 SacI G_AGCT'C Cuts at: 2211 3159 2211 Size: 948 4954 SacII CC_GC'GG Cuts at: 2196 2196 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2021 2621 2804 3250 4385 5066 5257 Size: 219 976 600 183 446 1135 681 191 Cuts at: 5257 826 Size: 1471 Fragments arranged by size: 1471 1135 976 681 600 446 219 191 183 SfoI GGC'GCC Cuts at: 2377 2377 Size: 5902 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3044 3229 1872 Size: 1172 185 4545 Fragments arranged by size: 4545 1172 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 3173 4006 4907 5169 820 Size: 197 926 177 1053 833 901 262 1553 Fragments arranged by size: 1553 1053 926 901 833 262 197 177 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SrfI GCCC'GGGC Cuts at: 3044 3044 Size: 5902 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 2137 1517 Size: 620 5282 StyI C'CwwG_G Cuts at: 513 1065 2004 2814 3341 513 Size: 552 939 810 527 3074 Fragments arranged by size: 3074 939 810 552 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 2309 2372 2411 2431 3929 3946 Size: 1255 723 6 63 39 20 1498 17 Cuts at: 3946 4099 4284 4729 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1498 1255 723 445 185 153 63 39 20 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2197 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 2120 1943 Size: 177 5725 XmaI C'CCGG_G Cuts at: 1870 3042 3227 1870 Size: 1172 185 4545 Fragments arranged by size: 4545 1172 185 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SanDI SapI SexAI SfiI SwaI