Restriction map for
pFN21AE0130
(Linear) (Six-base) MAPSORT of: KIEE0130.seq Check: 317 from: 1 to: 1197
KIEE0130.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 705 1197
Size: 705 492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 1197
Size: 76 1121
AlwNI CAG_nnn'CTG
Cuts at: 0 462 1197
Size: 462 735
ApaI G_GGCC'C
Cuts at: 0 262 879 1197
Size: 262 617 318
Fragments arranged by size:
617 318 262
ApaLI G'TGCA_C
Cuts at: 0 625 1197
Size: 625 572
ApoI r'AATT_y
Cuts at: 0 100 1197
Size: 100 1097
AvaI C'yCGr_G
Cuts at: 0 115 1037 1197
Size: 115 922 160
Fragments arranged by size:
922 160 115
BanI G'GyrC_C
Cuts at: 0 26 370 452 1033 1045 1197
Size: 26 344 82 581 12 152
Fragments arranged by size:
581 344 152 82 26 12
BanII G_rGCy'C
Cuts at: 0 206 262 879 962 1014 1154 1169 1197
Size: 206 56 617 83 52 140 15 28
Fragments arranged by size:
617 206 140 83 56 52 28 15
BbeI G_GCGC'C
Cuts at: 0 374 1197
Size: 374 823
BbvCI CC'TCA_GC
Cuts at: 0 1145 1197
Size: 1145 52
BglI GCCn_nnn'nGGC
Cuts at: 0 190 658 1197
Size: 190 468 539
Fragments arranged by size:
539 468 190
BlpI GC'TnA_GC
Cuts at: 0 743 1197
Size: 743 454
Bme1580I G_kGCm'C
Cuts at: 0 262 284 629 879 1038 1050 1197
Size: 262 22 345 250 159 12 147
Fragments arranged by size:
345 262 250 159 147 22 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 263 1197
Size: 263 934
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 783 1197
Size: 783 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1141 1197
Size: 1108 33 56
Fragments arranged by size:
1108 56 33
Bpu10I CC'TnA_GC
Cuts at: 0 291 1145 1197
Size: 291 854 52
Fragments arranged by size:
854 291 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1197
Size: 1153 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 567 938 1197
Size: 316 251 371 259
Fragments arranged by size:
371 316 259 251
BsaHI Gr'CG_yC
Cuts at: 0 371 1197
Size: 371 826
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 313 517 591 594 597 1111 1144
Size: 187 126 204 74 3 3 514 33
Cuts at: 1144 1162 1197
Size: 18 35
Fragments arranged by size:
514 204 187 126 74 35 33 18
3 3
BseYI C'CCAG_C
Cuts at: 0 954 1197
Size: 954 243
BsiEI CG_ry'CG
Cuts at: 0 210 318 846 1197
Size: 210 108 528 351
Fragments arranged by size:
528 351 210 108
BsiHKAI G_wGCw'C
Cuts at: 0 206 629 1154 1197
Size: 206 423 525 43
Fragments arranged by size:
525 423 206 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 769 1197
Size: 769 428
Bsp1286I G_dGCh'C
Cuts at: 0 206 262 284 629 879 962 1014 1038
Size: 206 56 22 345 250 83 52 24
Cuts at: 1038 1050 1154 1169 1197
Size: 12 104 15 28
Fragments arranged by size:
345 250 206 104 83 56 52 28
24 22 15 12
BsrBI CCG'CTC
Cuts at: 0 742 1197
Size: 742 455
BsrFI r'CCGG_y
Cuts at: 0 29 367 1030 1197
Size: 29 338 663 167
Fragments arranged by size:
663 338 167 29
BssSI C'ACGA_G
Cuts at: 0 622 1197
Size: 622 575
BstAPI GCAn_nnn'nTGC
Cuts at: 0 398 624 1197
Size: 398 226 573
Fragments arranged by size:
573 398 226
BstEII G'GTnAC_C
Cuts at: 0 6 1197
Size: 6 1191
BstXI CCAn_nnnn'nTGG
Cuts at: 0 694 1197
Size: 694 503
Bsu36I CC'TnA_GG
Cuts at: 0 127 856 1197
Size: 127 729 341
Fragments arranged by size:
729 341 127
BtgI C'CryG_G
Cuts at: 0 188 262 809 1197
Size: 188 74 547 388
Fragments arranged by size:
547 388 188 74
DrdI GACnn_nn'nnGTC
Cuts at: 0 214 1197
Size: 214 983
EaeI y'GGCC_r
Cuts at: 0 365 827 923 1197
Size: 365 462 96 274
Fragments arranged by size:
462 365 274 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 655 828 1197
Size: 655 173 369
Fragments arranged by size:
655 369 173
EcoICRI GAG'CTC
Cuts at: 0 204 1152 1197
Size: 204 948 45
Fragments arranged by size:
948 204 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1141 1197
Size: 1108 33 56
Fragments arranged by size:
1108 56 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 1123 1159 1197
Size: 685 438 36 38
Fragments arranged by size:
685 438 38 36
EcoO109I rG'GnC_Cy
Cuts at: 0 57 258 822 875 1096 1116 1197
Size: 57 201 564 53 221 20 81
Fragments arranged by size:
564 221 201 81 57 53 20
EcoRI G'AATT_C
Cuts at: 0 100 1197
Size: 100 1097
FspI TGC'GCA
Cuts at: 0 391 1197
Size: 391 806
HaeII r_GCGC'y
Cuts at: 0 374 1197
Size: 374 823
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1197
Size: 691 32 474
Fragments arranged by size:
691 474 32
HincII GTy'rAC
Cuts at: 0 45 219 1197
Size: 45 174 978
Fragments arranged by size:
978 174 45
KasI G'GCGC_C
Cuts at: 0 370 1197
Size: 370 827
MscI TGG'CCA
Cuts at: 0 829 1197
Size: 829 368
MslI CAynn'nnrTG
Cuts at: 0 538 724 1197
Size: 538 186 473
Fragments arranged by size:
538 473 186
MspA1I CmG'CkG
Cuts at: 0 190 1197
Size: 190 1007
NaeI GCC'GGC
Cuts at: 0 369 1197
Size: 369 828
NarI GG'CG_CC
Cuts at: 0 371 1197
Size: 371 826
NcoI C'CATG_G
Cuts at: 0 809 1197
Size: 809 388
NgoMIV G'CCGG_C
Cuts at: 0 367 1197
Size: 367 830
NspI r_CATG'y
Cuts at: 0 550 1197
Size: 550 647
PasI CC'CwG_GG
Cuts at: 0 236 375 434 496 1197
Size: 236 139 59 62 701
Fragments arranged by size:
701 236 139 62 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 1197
Size: 462 735
PfoI T'CCnGG_A
Cuts at: 0 77 332 475 1157 1178 1197
Size: 77 255 143 682 21 19
Fragments arranged by size:
682 255 143 77 21 19
PpuMI rG'GwC_Cy
Cuts at: 0 1096 1197
Size: 1096 101
PspOMI G'GGCC_C
Cuts at: 0 258 875 1197
Size: 258 617 322
Fragments arranged by size:
617 322 258
PspXI vC'TCGA_Gb
Cuts at: 0 115 1197
Size: 115 1082
PstI C_TGCA'G
Cuts at: 0 20 620 803 1197
Size: 20 600 183 394
Fragments arranged by size:
600 394 183 20
SacI G_AGCT'C
Cuts at: 0 206 1154 1197
Size: 206 948 43
Fragments arranged by size:
948 206 43
SacII CC_GC'GG
Cuts at: 0 191 1197
Size: 191 1006
SfcI C'TryA_G
Cuts at: 0 16 616 799 1197
Size: 16 600 183 398
Fragments arranged by size:
600 398 183 16
SfoI GGC'GCC
Cuts at: 0 372 1197
Size: 372 825
SmaI CCC'GGG
Cuts at: 0 1039 1197
Size: 1039 158
SmlI C'TyrA_G
Cuts at: 0 115 1168 1197
Size: 115 1053 29
Fragments arranged by size:
1053 115 29
SrfI GCCC'GGGC
Cuts at: 0 1039 1197
Size: 1039 158
StuI AGG'CCT
Cuts at: 0 132 1197
Size: 132 1065
StyI C'CwwG_G
Cuts at: 0 809 1197
Size: 809 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 304 367 406 426 1197
Size: 298 6 63 39 20 771
Fragments arranged by size:
771 298 63 39 20 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 1197
Size: 192 1005
XhoI C'TCGA_G
Cuts at: 0 115 1197
Size: 115 1082
XmaI C'CCGG_G
Cuts at: 0 1037 1197
Size: 1037 160
Enzymes that do cut:
AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssSI BstAPI BstEII
BstXI Bsu36I BtgI DrdI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI SacI
SacII SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EarI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TatI TstI Tth111I XbaI XmnI ZraI