Restriction map for
pFN21AB6489
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII AccI AccII AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BfrI BglI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI ItaI KasI Ksp632I
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspI SwaI TaqI TseI Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 12 GGTACC G^GTACC
AccIII 827 TCCGGA T^CCGGA
AclI 977 AACGTT AA^CGTT
AlwI 70 GATCC GGATC(4/5)
Asp718I 12 GGTACC G^GTACC
AvaII 118 GGACC G^G[AT]CC
BanI 12 GGTACC G^G[CT][GA]CC
BbsI 472 GAAGAC GAAGAC(2/6)
BcnI 528 CCGGG CC^[GC]GG
BfaI 892 CTAG C^TAG
BglII 964 AGATCT A^GATCT
BpmI 625 CTGGAG CTGGAG(16/14)
BpuAI 472 GAAGAC GAAGAC(2/6)
BsaI 570 GAGACC GGTCTC(1/5)
BseAI 827 TCCGGA T^CCGGA
BseRI 86 CTCCTC GAGGAG(10/8)
BsmAI 570 GAGAC GTCTC(1/5)
BsmFI 136 GTCCC GGGAC(10/14)
BsmI 495 GAATGC GAATGC(1/-1)
Bsp1407I 695 TGTACA T^GTACA
BspEI 827 TCCGGA T^CCGGA
BspHI 557 TCATGA T^CATGA
BsrDI 853 GCAATG GCAATG(2/0)
BsrGI 695 TGTACA T^GTACA
EcoT14I 73 CCAAGG C^C[AT][AT]GG
HinfI 772 GATTC G^A[ACGT]TC
KpnI 12 GGTACC GGTAC^C
MaeI 892 CTAG C^TAG
MroI 827 TCCGGA T^CCGGA
MseI 434 TTAA T^TAA
MslI 558 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 131 CAGCTG C[AC]G^C[GT]G
NciI 528 CCGGG CC^[GC]GG
PpuMI 117 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 977 AACGTT AA^CGTT
PstI 35 CTGCAG CTGCA^G
PvuII 131 CAGCTG CAG^CTG
RcaI 557 TCATGA T^CATGA
SbfI 34 CCTGCAGG CCTGCA^GG
Sse8387I 34 CCTGCAGG CCTGCA^GG
SspBI 695 TGTACA T^GTACA
StuI 76 AGGCCT AGG^CCT
StyI 73 CCAAGG C^C[AT][AT]GG
TfiI 772 GATTC G^A[AT]TC
Tru9I 434 TTAA T^TAA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 667 GCGG CCGC(-3/-1)
691 GCGG
AflIII 366 ACATGT A^C[GA][CT]GT
403 ACATGT
BciVI 175 GGATAC GTATCC(6/5)
230 GTATCC
BsaWI 242 TCCGGT [AT]^CCGG[AT]
827 TCCGGA
BsgI 631 GTGCAG GTGCAG(16/14)
684 GTGCAG
BspLU11I 366 ACATGT A^CATGT
403 ACATGT
BspMI 586 ACCTGC ACCTGC(4/8)
37 GCAGGT
BstXI 216 CCAGCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
286 CCAATAACATGG
BstYI 69 AGATCC [GA]^GATC[CT]
964 AGATCT
DpnII 70 GATC ^GATC
965 GATC
DraII 117 AGGACCC [GA]G^G[ACGT]CC[CT]
514 AGGCCCT
EcoO109I 117 AGGACCC [GA]G^G[ACGT]CC[CT]
514 AGGCCCT
HphI 40 GGTGA GGTGA(8/7)
650 TCACC
MaeII 461 ACGT A^CGT
978 ACGT
MboI 70 GATC ^GATC
965 GATC
MflI 69 AGATCC [GA]^GATC[CT]
964 AGATCT
NdeII 70 GATC ^GATC
965 GATC
NspI 366 ACATGT [GA]CATG^[CT]
403 ACATGT
PflMI 142 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
220 CCAGTTGGTGG
Sau3AI 70 GATC ^GATC
965 GATC
SfcI 35 CTGCAG C^T[GA][CT]AG
180 CTACAG
Tsp45I 532 GTCAC ^GT[GC]AC
649 GTCAC
TspRI 534 CACTG CA[GC]TG[ACGT][ACGT]^
634 CAGTG
Van91I 142 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
220 CCAGTTGGTGG
XcmI 216 CCAGCCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCAGACAAGAGCTGG
XhoII 69 AGATCC [GA]^GATC[CT]
964 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI AlwNI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII FokI
HaeIII HapII HpaII MaeIII MboII
MnlI MspI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI