(Circular) (Six-base) MAPSORT of: pFN21AE1081.seq Check: 8519 from: 1 to: 9184 pFN21AE1081 9184 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3318 3318 Size: 9184 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2307 3914 6994 278 Size: 53 83 186 936 771 1607 3080 2468 Fragments arranged by size: 3080 2468 1607 936 771 186 83 53 AccI GT'mk_AC Cuts at: 1937 3809 5371 6527 1937 Size: 1872 1562 1156 4594 Fragments arranged by size: 4594 1872 1562 1156 Acc65I G'GTAC_C Cuts at: 6505 6505 Size: 9184 AclI AA'CG_TT Cuts at: 1563 4776 7311 7684 1563 Size: 3213 2535 373 3063 Fragments arranged by size: 3213 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 2694 5952 7247 8631 8899 1632 Size: 861 201 3258 1295 1384 268 1917 Fragments arranged by size: 3258 1917 1384 1295 861 268 201 AflII C'TTAA_G Cuts at: 820 1017 3376 820 Size: 197 2359 6628 Fragments arranged by size: 6628 2359 197 AflIII A'CryG_T Cuts at: 1203 8083 9060 1203 Size: 6880 977 1327 Fragments arranged by size: 6880 1327 977 AgeI A'CCGG_T Cuts at: 8015 8015 Size: 9184 AhdI GACnn_n'nnGTC Cuts at: 5637 7913 5637 Size: 2276 6908 AleI CACnn'nnGTG Cuts at: 1166 3803 1166 Size: 2637 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9184 AlwNI CAG_nnn'CTG Cuts at: 2566 2990 6927 8499 2566 Size: 424 3937 1572 3251 Fragments arranged by size: 3937 3251 1572 424 ApaLI G'TGCA_C Cuts at: 5378 7241 8397 5378 Size: 1863 1156 6165 Fragments arranged by size: 6165 1863 1156 ApoI r'AATT_y Cuts at: 1493 2081 2700 2836 3115 3170 3565 3663 3730 Size: 588 619 136 279 55 395 98 67 Cuts at: 3730 4787 5061 5231 5319 5438 5897 6493 6719 Size: 1057 274 170 88 119 459 596 226 Cuts at: 6719 6871 8980 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1057 619 596 588 459 395 279 274 226 170 152 136 119 98 88 67 55 AseI AT'TA_AT Cuts at: 160 1032 7738 160 Size: 872 6706 1606 Fragments arranged by size: 6706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9184 AvaI C'yCGr_G Cuts at: 1870 1943 2598 6296 6405 6509 1870 Size: 73 655 3698 109 104 4545 Fragments arranged by size: 4545 3698 655 109 104 73 BamHI G'GATC_C Cuts at: 4709 6514 6881 4709 Size: 1805 367 7012 Fragments arranged by size: 7012 1805 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6053 6505 7960 618 Size: 325 217 627 4266 452 1455 1842 Fragments arranged by size: 4266 1842 1455 627 452 325 217 BanII G_rGCy'C Cuts at: 1391 2848 2867 5160 6503 1391 Size: 1457 19 2293 1343 4072 Fragments arranged by size: 4072 2293 1457 1343 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2883 3891 5101 5534 928 Size: 971 984 1008 1210 433 4578 Fragments arranged by size: 4578 1210 1008 984 971 433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7375 7409 1903 Size: 34 5438 34 3678 Fragments arranged by size: 5438 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7076 8286 7076 Size: 1210 7974 BclI T'GATC_A Cuts at: 1548 2454 5728 1548 Size: 906 3274 5004 Fragments arranged by size: 5004 3274 906 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7795 136 Size: 107 122 71 631 605 6123 1525 Fragments arranged by size: 6123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2272 4598 5764 9179 2272 Size: 2326 1166 3415 2277 Fragments arranged by size: 3415 2326 2277 1166 BlpI GC'TnA_GC Cuts at: 6601 6601 Size: 9184 Bme1580I G_kGCm'C Cuts at: 1790 5382 7245 8401 1790 Size: 3592 1863 1156 2573 Fragments arranged by size: 3592 2573 1863 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3797 6915 7873 Size: 416 53 83 399 24 2380 3118 958 Cuts at: 7873 442 Size: 1753 Fragments arranged by size: 3118 2380 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2631 3940 4852 2631 Size: 1309 912 6963 Fragments arranged by size: 6963 1309 912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2297 2881 2908 3877 5820 6366 7844 Size: 845 213 584 27 969 1943 546 1478 Cuts at: 7844 1239 Size: 2579 Fragments arranged by size: 2579 1943 1478 969 845 584 546 213 27 Bpu10I CC'TnA_GC Cuts at: 2567 5046 2567 Size: 2479 6705 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 2601 5293 7309 8174 8472 2159 Size: 442 2692 2016 865 298 2871 Fragments arranged by size: 2871 2692 2016 865 442 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3031 3445 7847 882 Size: 617 1532 414 4402 2219 Fragments arranged by size: 4402 2219 1532 617 414 BsaAI yAC'GTr Cuts at: 493 1204 6975 493 Size: 711 5771 2702 Fragments arranged by size: 5771 2702 711 BsaBI GATnn'nnATC Cuts at: 2911 3471 9178 2911 Size: 560 5707 2917 Fragments arranged by size: 5707 2917 560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3911 6991 7373 Size: 53 83 186 936 771 1607 3080 382 Cuts at: 7373 8008 275 Size: 635 1451 Fragments arranged by size: 3080 1607 1451 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7617 8015 8289 8436 8847 7617 Size: 398 274 147 411 7954 Fragments arranged by size: 7954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3065 3095 1178 Size: 30 165 30 1662 30 7267 Fragments arranged by size: 7267 1662 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2604 3618 3973 4296 5180 1186 Size: 1418 1014 355 323 884 5190 Fragments arranged by size: 5190 1418 1014 884 355 323 BseYI C'CCAG_C Cuts at: 3241 8387 8883 3241 Size: 5146 496 3542 Fragments arranged by size: 5146 3542 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3375 3620 1163 Size: 573 1639 245 6727 Fragments arranged by size: 6727 1639 573 245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6634 7395 8423 1540 Size: 275 186 4633 761 1028 2301 Fragments arranged by size: 4633 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2867 5382 7245 7330 8401 2867 Size: 2515 1863 85 1071 3650 Fragments arranged by size: 3650 2515 1863 1071 85 BsmI GAATG_Cn' Cuts at: 4390 5218 6247 6695 6788 4390 Size: 828 1029 448 93 6786 Fragments arranged by size: 6786 1029 828 448 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2848 2867 5160 5382 6503 7245 7330 Size: 399 1058 19 2293 222 1121 742 85 Cuts at: 7330 8401 1391 Size: 1071 2174 Fragments arranged by size: 2293 2174 1121 1071 1058 742 399 222 85 19 BspEI T'CCGG_A Cuts at: 8847 8847 Size: 9184 BspHI T'CATG_A Cuts at: 2076 5385 7073 8803 2076 Size: 3309 1688 1730 2457 Fragments arranged by size: 3309 2457 1730 1688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3318 4958 6539 844 Size: 2474 1640 1581 3489 Fragments arranged by size: 3489 2474 1640 1581 BsrBI CCG'CTC Cuts at: 1394 1823 7071 1394 Size: 429 5248 3507 Fragments arranged by size: 5248 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2756 5120 7679 7853 65 Size: 1183 1508 2364 2559 174 1396 Fragments arranged by size: 2559 2364 1508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7828 8015 1700 Size: 110 6018 187 2869 Fragments arranged by size: 6018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4496 6121 6183 96 Size: 4400 1625 62 3097 Fragments arranged by size: 4400 3097 1625 62 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9184 BssSI C'ACGA_G Cuts at: 3517 5679 7244 8256 3517 Size: 2162 1565 1012 4445 Fragments arranged by size: 4445 2162 1565 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9184 BstBI TT'CG_AA Cuts at: 3691 7988 3691 Size: 4297 4887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9184 BstXI CCAn_nnnn'nTGG Cuts at: 2040 2840 4357 5408 5977 6427 2040 Size: 800 1517 1051 569 450 4797 Fragments arranged by size: 4797 1517 1051 800 569 450 BstYI r'GATC_y Cuts at: 1972 2272 2894 3896 4598 4709 4781 5764 6514 Size: 300 622 1002 702 111 72 983 750 Cuts at: 6514 6881 7266 7283 8735 8821 8833 9179 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1002 983 750 702 622 385 367 346 300 111 86 72 17 12 BstZ17I GTA'TAC Cuts at: 3810 5372 3810 Size: 1562 7622 Bsu36I CC'TnA_GG Cuts at: 3456 4428 5192 3456 Size: 972 764 7448 Fragments arranged by size: 7448 972 764 BtgI C'CryG_G Cuts at: 513 1065 2004 6285 513 Size: 552 939 4281 3412 Fragments arranged by size: 4281 3412 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8445 Fragments arranged by size: 8445 683 56 BtsI GCAGTG_nn' Cuts at: 6386 6700 7494 7514 6386 Size: 314 794 20 8056 Fragments arranged by size: 8056 794 314 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3585 3620 529 Size: 35 3021 35 6093 Fragments arranged by size: 6093 3021 35 35 DraI TTT'AAA Cuts at: 6488 6488 Size: 9184 DraIII CAC_nnn'GTG Cuts at: 4863 4863 Size: 9184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3916 8191 809 Size: 507 2600 4275 1802 Fragments arranged by size: 4275 2600 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6041 6631 7520 Size: 54 1425 50 275 18 4211 590 889 Cuts at: 7520 9149 8 Size: 1629 43 Fragments arranged by size: 4211 1629 1425 889 590 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6631 1537 Size: 275 4819 4090 Fragments arranged by size: 4819 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2370 2469 3012 4356 4395 4581 5165 Size: 975 42 99 543 1344 39 186 584 Cuts at: 5165 5337 5928 6447 7114 1353 Size: 172 591 519 667 3423 Fragments arranged by size: 3423 1344 975 667 591 584 543 519 186 172 99 42 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7771 8145 8291 387 Size: 1064 138 6182 374 146 1280 Fragments arranged by size: 6182 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2865 2865 Size: 9184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2297 2493 2694 2881 2908 3877 Size: 393 452 213 196 201 187 27 969 Cuts at: 3877 5820 5952 6366 7247 7844 8631 8899 1239 Size: 1943 132 414 881 597 787 268 1524 Fragments arranged by size: 1943 1524 969 881 787 597 452 414 393 268 213 201 196 187 132 27 EcoO109I rG'GnC_Cy Cuts at: 6410 6470 6410 Size: 60 9124 EcoRI G'AATT_C Cuts at: 2836 6493 6871 2836 Size: 3657 378 5149 Fragments arranged by size: 5149 3657 378 EcoRV GAT'ATC Cuts at: 2649 2649 Size: 9184 FspI TGC'GCA Cuts at: 6014 7690 6014 Size: 1676 7508 FspAI rTGC'GCAy Cuts at: 6014 6014 Size: 9184 HaeII r_GCGC'y Cuts at: 1678 2566 8331 1678 Size: 888 5765 2531 Fragments arranged by size: 5765 2531 888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2849 2881 2987 Size: 32 135 32 692 32 553 32 106 Cuts at: 2987 3019 3134 3166 3182 3214 3851 3883 4013 Size: 32 115 32 16 32 637 32 130 Cuts at: 4013 4045 5623 5655 5713 5745 7825 7857 7899 Size: 32 1578 32 58 32 2080 32 42 Cuts at: 7899 7931 1373 Size: 32 2626 Fragments arranged by size: 2626 2080 1578 692 637 553 135 130 115 106 58 42 32 32 32 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 4308 4594 4978 6528 6774 669 Size: 988 281 2370 286 384 1550 246 3079 Fragments arranged by size: 3079 2370 1550 988 384 286 281 246 HindIII A'AGCT_T Cuts at: 748 2178 2546 2806 748 Size: 1430 368 260 7126 Fragments arranged by size: 7126 1430 368 260 HpaI GTT'AAC Cuts at: 6774 6774 Size: 9184 KpnI G_GTAC'C Cuts at: 6509 6509 Size: 9184 MfeI C'AATT_G Cuts at: 4989 6783 4989 Size: 1794 7390 MluI A'CGCG_T Cuts at: 9060 9060 Size: 9184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3647 4715 4759 8298 8482 1305 Size: 2342 1068 44 3539 184 2007 Fragments arranged by size: 3539 2342 2007 1068 184 44 MscI TGG'CCA Cuts at: 10 64 1489 1832 6043 10 Size: 54 1425 343 4211 3151 Fragments arranged by size: 4211 3151 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3803 5468 6129 7142 7501 7660 518 Size: 648 2637 1665 661 1013 359 159 2042 Fragments arranged by size: 2637 2042 1665 1013 661 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4757 6600 7277 8425 8670 1606 Size: 3151 1843 677 1148 245 2120 Fragments arranged by size: 3151 2120 1843 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 6285 513 Size: 552 939 4281 3412 Fragments arranged by size: 4281 3412 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9184 NotI GC'GGCC_GC Cuts at: 6631 6631 Size: 9184 NspI r_CATG'y Cuts at: 1132 1229 5849 6542 8087 1132 Size: 97 4620 693 1545 2229 Fragments arranged by size: 4620 2229 1545 693 97 PasI CC'CwG_GG Cuts at: 1348 4240 1348 Size: 2892 6292 PciI A'CATG_T Cuts at: 8083 8083 Size: 9184 PflMI CCAn_nnn'nTGG Cuts at: 1108 6039 6050 1108 Size: 4931 11 4242 Fragments arranged by size: 4931 4242 11 PfoI T'CCnGG_A Cuts at: 3855 3905 5222 3855 Size: 50 1317 7817 Fragments arranged by size: 7817 1317 50 PmeI GTTT'AAAC Cuts at: 6488 6488 Size: 9184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7253 7285 8766 8798 7253 Size: 32 1481 32 7639 Fragments arranged by size: 7639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 6410 6410 Size: 9184 PshAI GACnn'nnGTC Cuts at: 1613 4686 1613 Size: 3073 6111 PsiI TTA'TAA Cuts at: 4964 6754 4964 Size: 1790 7394 PstI C_TGCA'G Cuts at: 830 4342 4425 4923 4975 5055 6536 830 Size: 3512 83 498 52 80 1481 3478 Fragments arranged by size: 3512 3478 1481 498 83 80 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9184 PvuII CAG'CTG Cuts at: 4757 4757 Size: 9184 SacI G_AGCT'C Cuts at: 2867 2867 Size: 9184 SalI G'TCGA_C Cuts at: 1936 6526 1936 Size: 4590 4594 SanDI GG'GwC_CC Cuts at: 6410 6410 Size: 9184 SapI GCTCTTCn'nnn_ Cuts at: 2328 4395 5165 5928 2328 Size: 2067 770 763 5584 Fragments arranged by size: 5584 2067 770 763 SbfI CC_TGCA'GG Cuts at: 6536 6536 Size: 9184 ScaI AGT'ACT Cuts at: 4046 6103 7432 9144 4046 Size: 2057 1329 1712 4086 Fragments arranged by size: 4086 2057 1712 1329 SexAI A'CCwGG_T Cuts at: 5400 5400 Size: 9184 SfcI C'TryA_G Cuts at: 826 1045 3672 4267 4338 4421 4919 4971 5051 Size: 219 2627 595 71 83 498 52 80 Cuts at: 5051 6532 7667 8348 8539 826 Size: 1481 1135 681 191 1471 Fragments arranged by size: 2627 1481 1471 1135 681 595 498 219 191 83 80 71 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9184 SmaI CCC'GGG Cuts at: 1872 6407 6511 1872 Size: 4535 104 4545 Fragments arranged by size: 4545 4535 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2138 2598 2616 3376 5308 7288 Size: 197 926 195 460 18 760 1932 1980 Cuts at: 7288 8189 8451 820 Size: 901 262 1553 Fragments arranged by size: 1980 1932 1553 926 901 760 460 262 197 195 18 SnaBI TAC'GTA Cuts at: 493 6975 493 Size: 6482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9184 SphI G_CATG'C Cuts at: 1132 6542 1132 Size: 5410 3774 SspI AAT'ATT Cuts at: 5 52 2227 7108 5 Size: 47 2175 4881 2081 Fragments arranged by size: 4881 2175 2081 47 StuI AGG'CCT Cuts at: 1517 3704 1517 Size: 2187 6997 StyI C'CwwG_G Cuts at: 513 1065 2004 3552 4684 6285 6623 513 Size: 552 939 1548 1132 1601 338 3074 Fragments arranged by size: 3074 1601 1548 1132 939 552 338 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2574 6475 7211 7228 7381 7566 8011 Size: 1255 994 3901 736 17 153 185 445 Cuts at: 8011 325 Size: 1498 Fragments arranged by size: 3901 1498 1255 994 736 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3491 4044 4396 Size: 274 80 33 51 1443 1514 553 352 Cuts at: 4396 4496 6101 6121 6183 7430 9142 96 Size: 100 1605 20 62 1247 1712 138 Fragments arranged by size: 1712 1605 1514 1443 1247 553 352 274 138 100 80 62 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2953 2985 3065 3097 5497 5529 9144 9176 2953 Size: 32 80 32 2400 32 3615 32 2961 Fragments arranged by size: 3615 2961 2400 80 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4638 1316 Size: 3322 5862 XbaI T'CTAG_A Cuts at: 2731 6520 2731 Size: 3789 5395 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5127 5127 Size: 9184 XhoI C'TCGA_G Cuts at: 1943 2598 1943 Size: 655 8529 XmaI C'CCGG_G Cuts at: 1870 6405 6509 1870 Size: 4535 104 4545 Fragments arranged by size: 4545 4535 104 XmnI GAAnn'nnTTC Cuts at: 4885 7313 4885 Size: 2428 6756 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2305 3912 6992 276 Size: 53 83 186 936 771 1607 3080 2468 Fragments arranged by size: 3080 2468 1607 936 771 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI EcoNI FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI