Restriction map for
pFN21AE1081
(Linear) (Six-base) MAPSORT of: KIEE1081.seq Check: 5836 from: 1 to: 4479
KIEE1081.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1313 4479
Size: 1313 3166
AatII G_ACGT'C
Cuts at: 0 302 1909 4479
Size: 302 1607 2570
Fragments arranged by size:
2570 1607 302
AccI GT'mk_AC
Cuts at: 0 1804 3366 4479
Size: 1804 1562 1113
Fragments arranged by size:
1804 1562 1113
AclI AA'CG_TT
Cuts at: 0 2771 4479
Size: 2771 1708
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 689 3947 4479
Size: 488 201 3258 532
Fragments arranged by size:
3258 532 488 201
AflII C'TTAA_G
Cuts at: 0 1371 4479
Size: 1371 3108
AhdI GACnn_n'nnGTC
Cuts at: 0 3632 4479
Size: 3632 847
AleI CACnn'nnGTG
Cuts at: 0 1798 4479
Size: 1798 2681
AlwNI CAG_nnn'CTG
Cuts at: 0 561 985 4479
Size: 561 424 3494
Fragments arranged by size:
3494 561 424
ApaLI G'TGCA_C
Cuts at: 0 3373 4479
Size: 3373 1106
ApoI r'AATT_y
Cuts at: 0 76 695 831 1110 1165 1560 1658 1725
Size: 76 619 136 279 55 395 98 67
Cuts at: 1725 2782 3056 3226 3314 3433 3892 4479
Size: 1057 274 170 88 119 459 587
Fragments arranged by size:
1057 619 587 459 395 279 274 170
136 119 98 88 76 67 55
AvaI C'yCGr_G
Cuts at: 0 593 4291 4400 4479
Size: 593 3698 109 79
Fragments arranged by size:
3698 593 109 79
BamHI G'GATC_C
Cuts at: 0 2704 4479
Size: 2704 1775
BanI G'GyrC_C
Cuts at: 0 4048 4479
Size: 4048 431
BanII G_rGCy'C
Cuts at: 0 843 862 3155 4479
Size: 843 19 2293 1324
Fragments arranged by size:
2293 1324 843 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 878 1886 3096 3529 4479
Size: 878 1008 1210 433 950
Fragments arranged by size:
1210 1008 950 878 433
BclI T'GATC_A
Cuts at: 0 449 3723 4479
Size: 449 3274 756
Fragments arranged by size:
3274 756 449
BglII A'GATC_T
Cuts at: 0 267 2593 3759 4479
Size: 267 2326 1166 720
Fragments arranged by size:
2326 1166 720 267
Bme1580I G_kGCm'C
Cuts at: 0 3377 4479
Size: 3377 1102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1792 4479
Size: 1792 2687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 626 1935 2847 4479
Size: 626 1309 912 1632
Fragments arranged by size:
1632 1309 912 626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 292 876 903 1872 3815 4361 4479
Size: 79 213 584 27 969 1943 546 118
Fragments arranged by size:
1943 969 584 546 213 118 79 27
Bpu10I CC'TnA_GC
Cuts at: 0 562 3041 4479
Size: 562 2479 1438
Fragments arranged by size:
2479 1438 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 596 3288 4479
Size: 154 442 2692 1191
Fragments arranged by size:
2692 1191 442 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 1026 1440 4479
Size: 1026 414 3039
Fragments arranged by size:
3039 1026 414
BsaBI GATnn'nnATC
Cuts at: 0 906 1466 4479
Size: 906 560 3013
Fragments arranged by size:
3013 906 560
BsaHI Gr'CG_yC
Cuts at: 0 299 1906 4479
Size: 299 1607 2573
Fragments arranged by size:
2573 1607 299
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1060 1090 4479
Size: 1060 30 3389
Fragments arranged by size:
3389 1060 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 599 1613 1968 2291 3175 4479
Size: 599 1014 355 323 884 1304
Fragments arranged by size:
1304 1014 884 599 355 323
BseYI C'CCAG_C
Cuts at: 0 1236 4479
Size: 1236 3243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1370 1615 4479
Size: 1370 245 2864
Fragments arranged by size:
2864 1370 245
BsiHKAI G_wGCw'C
Cuts at: 0 862 3377 4479
Size: 862 2515 1102
Fragments arranged by size:
2515 1102 862
BsmI GAATG_Cn'
Cuts at: 0 2385 3213 4242 4479
Size: 2385 828 1029 237
Fragments arranged by size:
2385 1029 828 237
Bsp1286I G_dGCh'C
Cuts at: 0 843 862 3155 3377 4479
Size: 843 19 2293 222 1102
Fragments arranged by size:
2293 1102 843 222 19
BspHI T'CATG_A
Cuts at: 0 71 3380 4479
Size: 71 3309 1099
Fragments arranged by size:
3309 1099 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1313 2953 4479
Size: 1313 1640 1526
Fragments arranged by size:
1640 1526 1313
BsrDI GCAATG_nn'
Cuts at: 0 751 3115 4479
Size: 751 2364 1364
Fragments arranged by size:
2364 1364 751
BsrGI T'GTAC_A
Cuts at: 0 2491 4116 4178 4479
Size: 2491 1625 62 301
Fragments arranged by size:
2491 1625 301 62
BssSI C'ACGA_G
Cuts at: 0 1512 3674 4479
Size: 1512 2162 805
Fragments arranged by size:
2162 1512 805
BstBI TT'CG_AA
Cuts at: 0 1686 4479
Size: 1686 2793
BstXI CCAn_nnnn'nTGG
Cuts at: 0 35 835 2352 3403 3972 4422 4479
Size: 35 800 1517 1051 569 450 57
Fragments arranged by size:
1517 1051 800 569 450 57 35
BstYI r'GATC_y
Cuts at: 0 267 889 1891 2593 2704 2776 3759 4479
Size: 267 622 1002 702 111 72 983 720
Fragments arranged by size:
1002 983 720 702 622 267 111 72
BstZ17I GTA'TAC
Cuts at: 0 1805 3367 4479
Size: 1805 1562 1112
Fragments arranged by size:
1805 1562 1112
Bsu36I CC'TnA_GG
Cuts at: 0 1451 2423 3187 4479
Size: 1451 972 764 1292
Fragments arranged by size:
1451 1292 972 764
BtgI C'CryG_G
Cuts at: 0 4280 4479
Size: 4280 199
BtsI GCAGTG_nn'
Cuts at: 0 4381 4479
Size: 4381 98
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1580 1615 4479
Size: 1580 35 2864
Fragments arranged by size:
2864 1580 35
DraIII CAC_nnn'GTG
Cuts at: 0 2858 4479
Size: 2858 1621
DrdI GACnn_nn'nnGTC
Cuts at: 0 1911 4479
Size: 1911 2568
EaeI y'GGCC_r
Cuts at: 0 4036 4479
Size: 4036 443
EarI CTCTTCn'nnn_
Cuts at: 0 323 365 464 1007 2351 2390 2576 3160
Size: 323 42 99 543 1344 39 186 584
Cuts at: 3160 3332 3923 4442 4479
Size: 172 591 519 37
Fragments arranged by size:
1344 591 584 543 519 323 186 172
99 42 39 37
EcoICRI GAG'CTC
Cuts at: 0 860 4479
Size: 860 3619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 292 488 689 876 903 1872 3815
Size: 79 213 196 201 187 27 969 1943
Cuts at: 3815 3947 4361 4479
Size: 132 414 118
Fragments arranged by size:
1943 969 414 213 201 196 187 132
118 79 27
EcoO109I rG'GnC_Cy
Cuts at: 0 4405 4465 4479
Size: 4405 60 14
Fragments arranged by size:
4405 60 14
EcoRI G'AATT_C
Cuts at: 0 831 4479
Size: 831 3648
EcoRV GAT'ATC
Cuts at: 0 644 4479
Size: 644 3835
FspI TGC'GCA
Cuts at: 0 4009 4479
Size: 4009 470
FspAI rTGC'GCAy
Cuts at: 0 4009 4479
Size: 4009 470
HaeII r_GCGC'y
Cuts at: 0 561 4479
Size: 561 3918
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 844 876 982 1014 1129 1161
Size: 259 32 553 32 106 32 115 32
Cuts at: 1161 1177 1209 1846 1878 2008 2040 3618 3650
Size: 16 32 637 32 130 32 1578 32
Cuts at: 3650 3708 3740 4479
Size: 58 32 739
Fragments arranged by size:
1578 739 637 553 259 130 115 106
58 32 32 32 32 32 32 32
32 32 16
HincII GTy'rAC
Cuts at: 0 2303 2589 2973 4479
Size: 2303 286 384 1506
Fragments arranged by size:
2303 1506 384 286
HindIII A'AGCT_T
Cuts at: 0 173 541 801 4479
Size: 173 368 260 3678
Fragments arranged by size:
3678 368 260 173
MfeI C'AATT_G
Cuts at: 0 2984 4479
Size: 2984 1495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1642 2710 2754 4479
Size: 1642 1068 44 1725
Fragments arranged by size:
1725 1642 1068 44
MscI TGG'CCA
Cuts at: 0 4038 4479
Size: 4038 441
MslI CAynn'nnrTG
Cuts at: 0 1798 3463 4124 4479
Size: 1798 1665 661 355
Fragments arranged by size:
1798 1665 661 355
MspA1I CmG'CkG
Cuts at: 0 2752 4479
Size: 2752 1727
NcoI C'CATG_G
Cuts at: 0 4280 4479
Size: 4280 199
NspI r_CATG'y
Cuts at: 0 3844 4479
Size: 3844 635
PasI CC'CwG_GG
Cuts at: 0 2235 4479
Size: 2235 2244
PflMI CCAn_nnn'nTGG
Cuts at: 0 4034 4045 4479
Size: 4034 11 434
Fragments arranged by size:
4034 434 11
PfoI T'CCnGG_A
Cuts at: 0 1850 1900 3217 4479
Size: 1850 50 1317 1262
Fragments arranged by size:
1850 1317 1262 50
PpuMI rG'GwC_Cy
Cuts at: 0 4405 4479
Size: 4405 74
PshAI GACnn'nnGTC
Cuts at: 0 2681 4479
Size: 2681 1798
PsiI TTA'TAA
Cuts at: 0 2959 4479
Size: 2959 1520
PstI C_TGCA'G
Cuts at: 0 2337 2420 2918 2970 3050 4479
Size: 2337 83 498 52 80 1429
Fragments arranged by size:
2337 1429 498 83 80 52
PvuII CAG'CTG
Cuts at: 0 2752 4479
Size: 2752 1727
SacI G_AGCT'C
Cuts at: 0 862 4479
Size: 862 3617
SanDI GG'GwC_CC
Cuts at: 0 4405 4479
Size: 4405 74
SapI GCTCTTCn'nnn_
Cuts at: 0 323 2390 3160 3923 4479
Size: 323 2067 770 763 556
Fragments arranged by size:
2067 770 763 556 323
ScaI AGT'ACT
Cuts at: 0 2041 4098 4479
Size: 2041 2057 381
Fragments arranged by size:
2057 2041 381
SexAI A'CCwGG_T
Cuts at: 0 3395 4479
Size: 3395 1084
SfcI C'TryA_G
Cuts at: 0 1667 2262 2333 2416 2914 2966 3046 4479
Size: 1667 595 71 83 498 52 80 1433
Fragments arranged by size:
1667 1433 595 498 83 80 71 52
SmaI CCC'GGG
Cuts at: 0 4402 4479
Size: 4402 77
SmlI C'TyrA_G
Cuts at: 0 133 593 611 1371 3303 4479
Size: 133 460 18 760 1932 1176
Fragments arranged by size:
1932 1176 760 460 133 18
SspI AAT'ATT
Cuts at: 0 222 4479
Size: 222 4257
StuI AGG'CCT
Cuts at: 0 1699 4479
Size: 1699 2780
StyI C'CwwG_G
Cuts at: 0 1547 2679 4280 4479
Size: 1547 1132 1601 199
Fragments arranged by size:
1601 1547 1132 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 569 4470 4479
Size: 569 3901 9
Fragments arranged by size:
3901 569 9
TatI w'GTAC_w
Cuts at: 0 1486 2039 2391 2491 4096 4116 4178 4479
Size: 1486 553 352 100 1605 20 62 301
Fragments arranged by size:
1605 1486 553 352 301 100 62 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 948 980 1060 1092 3492 3524 4479
Size: 948 32 80 32 2400 32 955
Fragments arranged by size:
2400 955 948 80 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2633 4479
Size: 2633 1846
XbaI T'CTAG_A
Cuts at: 0 726 4479
Size: 726 3753
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3122 4479
Size: 3122 1357
XhoI C'TCGA_G
Cuts at: 0 593 4479
Size: 593 3886
XmaI C'CCGG_G
Cuts at: 0 4400 4479
Size: 4400 79
XmnI GAAnn'nnTTC
Cuts at: 0 2880 4479
Size: 2880 1599
ZraI GAC'GTC
Cuts at: 0 300 1907 4479
Size: 300 1607 2572
Fragments arranged by size:
2572 1607 300
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AhdI AleI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BclI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI FspAI HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PshAI PsiI PstI PvuII SacI SanDI SapI
ScaI SexAI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflIII AgeI AlfI AloI ApaI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BfrBI BglI BlpI BmgBI BmtI BsaAI BsaWI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstAPI BstEII
BtgZI ClaI DraI EagI EciI EcoNI FalI FseI
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI