(Circular) (Six-base) MAPSORT of: pFN21AB6412.seq Check: 317 from: 1 to: 5710 pFN21AB6412 5710 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2057 2057 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2445 2712 2821 3773 5157 5425 1632 Size: 813 267 109 952 1384 268 1917 Fragments arranged by size: 1917 1384 952 813 268 267 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 2153 2502 2826 1166 Size: 987 349 324 4050 Fragments arranged by size: 4050 987 349 324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2407 2437 3453 5025 2407 Size: 30 1016 1572 3092 Fragments arranged by size: 3092 1572 1016 30 ApaI G_GGCC'C Cuts at: 2609 2609 Size: 5710 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2530 3035 1870 Size: 73 587 505 4545 Fragments arranged by size: 4545 587 505 73 BamHI G'GATC_C Cuts at: 2457 3040 3407 2457 Size: 583 367 4760 Fragments arranged by size: 4760 583 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2397 3031 4486 618 Size: 325 217 627 610 634 1455 1842 Fragments arranged by size: 1842 1455 634 627 610 325 217 BanII G_rGCy'C Cuts at: 1391 2537 2609 3029 1391 Size: 1146 72 420 4072 Fragments arranged by size: 4072 1146 420 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 928 Size: 971 326 4413 Fragments arranged by size: 4413 971 326 BbvCI CC'TCA_GC Cuts at: 2928 2928 Size: 5710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BfrBI ATG'CAT Cuts at: 2355 2355 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2609 3771 4927 1790 Size: 819 1162 1156 2573 Fragments arranged by size: 2573 1162 1156 819 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3014 3014 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2636 4370 1239 Size: 965 432 1734 2579 Fragments arranged by size: 2579 1734 965 432 Bpu10I CC'TnA_GC Cuts at: 2234 2928 2234 Size: 694 5016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2479 2832 3517 3899 Size: 53 83 186 936 946 353 685 382 Cuts at: 3899 4534 275 Size: 635 1451 Fragments arranged by size: 1451 946 936 685 635 382 353 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2677 2916 2990 1186 Size: 1491 239 74 3906 Fragments arranged by size: 3906 1491 239 74 BseYI C'CCAG_C Cuts at: 2056 4913 5409 2056 Size: 2857 496 2357 Fragments arranged by size: 2857 2357 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 3160 3921 4949 1540 Size: 275 186 153 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 2357 3221 3314 2357 Size: 864 93 4753 Fragments arranged by size: 4753 864 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2537 2609 3029 3771 3856 4927 1391 Size: 399 747 72 420 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 420 399 85 72 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 3065 844 Size: 1213 1008 3489 Fragments arranged by size: 3489 1213 1008 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2394 2747 4354 4541 1700 Size: 110 584 353 1607 187 2869 Fragments arranged by size: 2869 1607 584 353 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 2009 2446 1184 Size: 825 437 4448 Fragments arranged by size: 4448 825 437 BstXI CCAn_nnnn'nTGG Cuts at: 2064 2504 2064 Size: 440 5270 BstYI r'GATC_y Cuts at: 1972 2457 3040 3407 3792 3809 5261 5347 5359 Size: 485 583 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 583 485 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2503 2677 513 Size: 552 939 499 174 3546 Fragments arranged by size: 3546 939 552 499 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 2090 2417 2920 3226 4020 4040 2090 Size: 327 503 306 794 20 3760 Fragments arranged by size: 3760 794 503 327 306 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2900 4717 809 Size: 507 1584 1817 1802 Fragments arranged by size: 1817 1802 1584 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2314 2641 3157 Size: 54 1425 50 275 18 484 327 516 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 516 484 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2172 2688 3640 1353 Size: 756 63 516 952 3423 Fragments arranged by size: 3423 952 756 516 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2445 2636 2712 2821 3773 4370 Size: 393 572 241 191 76 109 952 597 Cuts at: 4370 5157 5425 1239 Size: 787 268 1524 Fragments arranged by size: 1524 952 787 597 572 393 268 241 191 109 76 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2270 2129 Size: 141 5569 EcoO109I rG'GnC_Cy Cuts at: 2068 2134 2229 2605 2720 2068 Size: 66 95 376 115 5058 Fragments arranged by size: 5058 376 115 95 66 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 4857 1678 Size: 3179 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 4351 4383 4425 Size: 32 135 32 549 32 2198 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 2198 549 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2502 2826 2853 3668 4027 4186 Size: 648 987 349 324 27 815 359 159 Cuts at: 4186 518 Size: 2042 Fragments arranged by size: 2042 987 815 648 359 349 324 159 27 MspA1I CmG'CkG Cuts at: 1606 2020 2029 2658 3126 3803 4951 5196 1606 Size: 414 9 629 468 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 629 468 414 245 9 NaeI GCC'GGC Cuts at: 2396 2396 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 2004 2677 513 Size: 552 939 673 3546 Fragments arranged by size: 3546 939 673 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NgoMIV G'CCGG_C Cuts at: 2394 2394 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NsiI A_TGCA'T Cuts at: 2357 2357 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2083 2819 3068 4613 1132 Size: 97 854 736 249 1545 2229 Fragments arranged by size: 2229 1545 854 736 249 97 PasI CC'CwG_GG Cuts at: 1348 2125 1348 Size: 777 4933 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2985 1108 Size: 1877 3833 PfoI T'CCnGG_A Cuts at: 2460 2613 2460 Size: 153 5557 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2229 2134 Size: 95 5615 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspOMI G'GGCC_C Cuts at: 2605 2605 Size: 5710 PstI C_TGCA'G Cuts at: 830 2144 2412 3062 830 Size: 1314 268 650 3478 Fragments arranged by size: 3478 1314 650 268 PvuI CG_AT'CG Cuts at: 2001 2154 2001 Size: 153 5557 PvuII CAG'CTG Cuts at: 2658 2658 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SapI GCTCTTCn'nnn_ Cuts at: 2172 2172 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2140 2408 3058 4193 4874 5065 826 Size: 219 1095 268 650 1135 681 191 1471 Fragments arranged by size: 1471 1135 1095 681 650 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 2532 3037 1872 Size: 660 505 4545 Fragments arranged by size: 4545 660 505 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 2083 3068 1132 Size: 951 985 3774 Fragments arranged by size: 3774 985 951 SrfI GCCC'GGGC Cuts at: 2532 2532 Size: 5710 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2677 3149 513 Size: 552 939 673 472 3074 Fragments arranged by size: 3074 939 673 552 472 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2690 2816 2904 3737 3754 3907 4092 Size: 1255 1110 126 88 833 17 153 185 Cuts at: 4092 4537 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1110 833 445 185 153 126 88 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2867 3956 5668 Size: 274 80 33 51 1443 890 1089 1712 Cuts at: 5668 96 Size: 138 Fragments arranged by size: 1712 1443 1089 890 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2309 2912 2309 Size: 603 5107 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 2530 3035 1870 Size: 660 505 4545 Fragments arranged by size: 4545 660 505 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI