(Circular) (Six-base) MAPSORT of: pFN21AE0351.seq Check: 3837 from: 1 to: 7159 pFN21AE0351 7159 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2890 4301 2890 Size: 1411 5748 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4969 278 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2159 4502 1937 Size: 222 2343 4594 Fragments arranged by size: 4594 2343 222 Acc65I G'GTAC_C Cuts at: 2718 2962 4480 2718 Size: 244 1518 5397 Fragments arranged by size: 5397 1518 244 AclI AA'CG_TT Cuts at: 1563 5286 5659 1563 Size: 3723 373 3063 Fragments arranged by size: 3723 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 2939 3058 3497 4436 5222 6606 6874 Size: 959 348 119 439 939 786 1384 268 Cuts at: 6874 1632 Size: 1917 Fragments arranged by size: 1917 1384 959 939 786 439 348 268 119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6962 AflIII A'CryG_T Cuts at: 1203 6058 7035 1203 Size: 4855 977 1327 Fragments arranged by size: 4855 1327 977 AgeI A'CCGG_T Cuts at: 5990 5990 Size: 7159 AhdI GACnn_n'nnGTC Cuts at: 5888 5888 Size: 7159 AleI CACnn'nnGTG Cuts at: 1166 2528 1166 Size: 1362 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2839 1184 Size: 1655 5504 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 2399 Size: 32 7127 AlwNI CAG_nnn'CTG Cuts at: 2618 2929 3046 3337 4402 4902 6474 2618 Size: 311 117 291 1065 500 1572 3303 Fragments arranged by size: 3303 1572 1065 500 311 291 117 ApaI G_GGCC'C Cuts at: 4081 4081 Size: 7159 ApaLI G'TGCA_C Cuts at: 2843 5216 6372 2843 Size: 2373 1156 3630 Fragments arranged by size: 3630 2373 1156 ApoI r'AATT_y Cuts at: 1493 4468 4694 4846 6955 1493 Size: 2975 226 152 2109 1697 Fragments arranged by size: 2975 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5713 160 Size: 872 4681 1606 Fragments arranged by size: 4681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 AvaI C'yCGr_G Cuts at: 1870 1943 2478 3000 3168 3706 4080 4098 4169 Size: 73 535 522 168 538 374 18 71 Cuts at: 4169 4484 1870 Size: 315 4545 Fragments arranged by size: 4545 538 535 522 374 315 168 73 71 18 BamHI G'GATC_C Cuts at: 3546 4489 4856 3546 Size: 943 367 5849 Fragments arranged by size: 5849 943 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2667 2718 2962 2996 4385 Size: 325 217 627 880 51 244 34 1389 Cuts at: 4385 4480 5935 618 Size: 95 1455 1842 Fragments arranged by size: 1842 1455 1389 880 627 325 244 217 95 51 34 BanII G_rGCy'C Cuts at: 1391 2681 3507 3558 3696 3927 4081 4260 4478 Size: 1290 826 51 138 231 154 179 218 Cuts at: 4478 1391 Size: 4072 Fragments arranged by size: 4072 1290 826 231 218 179 154 138 51 BbeI G_GCGC'C Cuts at: 2671 2671 Size: 7159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4338 928 Size: 971 2439 3749 Fragments arranged by size: 3749 2439 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 5350 5384 1903 Size: 34 2231 34 1148 34 3678 Fragments arranged by size: 3678 2231 1148 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5051 6261 5051 Size: 1210 5949 BclI T'GATC_A Cuts at: 1548 1548 Size: 7159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2737 5770 136 Size: 107 122 71 631 605 1065 3033 1525 Fragments arranged by size: 3033 1525 1065 631 605 122 107 71 BglII A'GATC_T Cuts at: 7154 7154 Size: 7159 BlpI GC'TnA_GC Cuts at: 2273 2660 3252 4576 2273 Size: 387 592 1324 4856 Fragments arranged by size: 4856 1324 592 387 Bme1580I G_kGCm'C Cuts at: 1790 2612 2733 2847 3001 4081 4453 5220 6376 Size: 822 121 114 154 1080 372 767 1156 Cuts at: 6376 1790 Size: 2573 Fragments arranged by size: 2573 1156 1080 822 767 372 154 121 114 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4890 5848 442 Size: 416 53 83 399 24 3473 958 1753 Fragments arranged by size: 3473 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7159 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2288 2573 2579 2665 3167 3215 3260 Size: 872 177 285 6 86 502 48 45 Cuts at: 3260 3320 3440 3604 3746 3806 3815 4244 5819 Size: 60 120 164 142 60 9 429 1575 Cuts at: 5819 1239 Size: 2579 Fragments arranged by size: 2579 1575 872 502 429 285 177 164 142 120 86 60 60 48 45 9 6 Bpu10I CC'TnA_GC Cuts at: 2885 3501 2885 Size: 616 6543 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2795 3412 5284 6149 6447 2795 Size: 617 1872 865 298 3507 Fragments arranged by size: 3507 1872 865 617 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2937 5822 882 Size: 617 1438 2885 2219 Fragments arranged by size: 2885 2219 1438 617 BsaAI yAC'GTr Cuts at: 493 1204 2143 3096 4950 493 Size: 711 939 953 1854 2702 Fragments arranged by size: 2702 1854 953 939 711 BsaBI GATnn'nnATC Cuts at: 2502 7153 2502 Size: 4651 2508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2668 3945 4966 5348 Size: 53 83 186 936 1135 1277 1021 382 Cuts at: 5348 5983 275 Size: 635 1451 Fragments arranged by size: 1451 1277 1135 1021 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2364 4422 5592 5990 6264 6411 6822 2364 Size: 2058 1170 398 274 147 411 2701 Fragments arranged by size: 2701 2058 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 3581 3611 3719 Size: 30 165 30 597 30 1551 30 108 Cuts at: 3719 3749 3955 3985 1178 Size: 30 206 30 4352 Fragments arranged by size: 4352 1551 597 206 165 108 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 2414 2689 2846 2885 3006 3556 3706 Size: 1225 3 275 157 39 121 550 150 Cuts at: 3706 1186 Size: 4639 Fragments arranged by size: 4639 1225 550 275 157 150 121 39 3 BseYI C'CCAG_C Cuts at: 2109 2714 3046 3902 4109 6362 6858 2109 Size: 605 332 856 207 2253 496 2410 Fragments arranged by size: 2410 2253 856 605 496 332 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2886 3296 3821 4172 4391 1163 Size: 573 1150 410 525 351 219 3931 Fragments arranged by size: 3931 1150 573 525 410 351 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2163 4609 5370 6398 1540 Size: 275 186 162 2446 761 1028 2301 Fragments arranged by size: 2446 2301 1028 761 275 186 162 BsiHKAI G_wGCw'C Cuts at: 2525 2681 2847 3177 3507 4260 5220 5305 6376 Size: 156 166 330 330 753 960 85 1071 Cuts at: 6376 2525 Size: 3308 Fragments arranged by size: 3308 1071 960 753 330 330 166 156 85 BsmI GAATG_Cn' Cuts at: 4145 4670 4763 4145 Size: 525 93 6541 Fragments arranged by size: 6541 525 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2525 2612 2681 2733 2847 3001 3177 Size: 399 735 87 69 52 114 154 176 Cuts at: 3177 3507 3558 3696 3927 4081 4260 4453 4478 Size: 330 51 138 231 154 179 193 25 Cuts at: 4478 5220 5305 6376 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 399 330 231 193 179 176 154 154 138 114 87 85 69 52 51 25 BspEI T'CCGG_A Cuts at: 2364 6822 2364 Size: 4458 2701 BspHI T'CATG_A Cuts at: 5048 6778 5048 Size: 1730 5429 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2112 2890 2955 3615 4301 4514 844 Size: 1228 40 778 65 660 686 213 3489 Fragments arranged by size: 3489 1228 778 686 660 213 65 40 BsrBI CCG'CTC Cuts at: 1394 1823 2664 5046 1394 Size: 429 841 2382 3507 Fragments arranged by size: 3507 2382 841 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2695 5654 5828 65 Size: 1183 1447 2959 174 1396 Fragments arranged by size: 2959 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5803 5990 1700 Size: 110 3993 187 2869 Fragments arranged by size: 3993 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7159 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7159 BssSI C'ACGA_G Cuts at: 3733 5219 6231 3733 Size: 1486 1012 4661 Fragments arranged by size: 4661 1486 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 794 Size: 2135 5024 BstBI TT'CG_AA Cuts at: 5963 5963 Size: 7159 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7159 BstYI r'GATC_y Cuts at: 1972 3546 3561 4276 4489 4856 5241 5258 6710 Size: 1574 15 715 213 367 385 17 1452 Cuts at: 6710 6796 6808 7154 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1574 1452 715 385 367 346 213 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2672 2735 2829 4116 4403 2672 Size: 63 94 1287 287 5428 Fragments arranged by size: 5428 1287 287 94 63 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5668 Fragments arranged by size: 5668 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6420 Fragments arranged by size: 6420 683 56 BtsI GCAGTG_nn' Cuts at: 4049 4675 5469 5489 4049 Size: 626 794 20 5719 Fragments arranged by size: 5719 794 626 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3256 3291 529 Size: 35 2692 35 4397 Fragments arranged by size: 4397 2692 35 35 DraI TTT'AAA Cuts at: 3772 4463 3772 Size: 691 6468 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6166 809 Size: 507 4850 1802 Fragments arranged by size: 4850 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2838 2907 Size: 54 1425 50 275 18 205 803 69 Cuts at: 2907 2982 3022 3180 3316 3986 4267 4606 5495 Size: 75 40 158 136 670 281 339 889 Cuts at: 5495 7124 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 803 670 339 281 275 205 158 136 75 69 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 4606 1537 Size: 275 2794 4090 Fragments arranged by size: 4090 2794 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3512 4154 5089 1353 Size: 2159 642 935 3423 Fragments arranged by size: 3423 2159 935 642 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5746 6120 6266 387 Size: 1064 138 4157 374 146 1280 Fragments arranged by size: 4157 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2679 3505 4258 2679 Size: 826 753 5580 Fragments arranged by size: 5580 826 753 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2288 2573 2579 2591 2665 2939 Size: 393 479 177 285 6 12 74 274 Cuts at: 2939 3058 3167 3215 3260 3320 3440 3497 3604 Size: 119 109 48 45 60 120 57 107 Cuts at: 3604 3746 3806 3815 4244 4436 5222 5819 6606 Size: 142 60 9 429 192 786 597 787 Cuts at: 6606 6874 1239 Size: 268 1524 Fragments arranged by size: 1524 787 786 597 479 429 393 285 274 268 192 177 142 120 119 109 107 74 60 60 57 48 45 12 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2921 2951 2921 Size: 30 7129 EcoO109I rG'GnC_Cy Cuts at: 2086 2577 2739 2914 2926 3121 3142 3152 3486 Size: 491 162 175 12 195 21 10 334 Cuts at: 3486 3711 3792 3826 4174 4399 2086 Size: 225 81 34 348 225 4846 Fragments arranged by size: 4846 491 348 334 225 225 195 175 162 81 34 21 12 10 EcoRI G'AATT_C Cuts at: 4468 4846 4468 Size: 378 6781 FspI TGC'GCA Cuts at: 5665 5665 Size: 7159 HaeII r_GCGC'y Cuts at: 1678 2671 3217 3293 3758 4345 6306 1678 Size: 993 546 76 465 587 1961 2531 Fragments arranged by size: 2531 1961 993 587 546 465 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2276 2308 5800 Size: 32 135 32 428 32 244 32 3492 Cuts at: 5800 5832 5874 5906 1373 Size: 32 42 32 2626 Fragments arranged by size: 3492 2626 428 244 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2160 4503 4749 669 Size: 988 281 222 2343 246 3079 Fragments arranged by size: 3079 2343 988 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 7159 HpaI GTT'AAC Cuts at: 4749 4749 Size: 7159 KasI G'GCGC_C Cuts at: 2667 2667 Size: 7159 KpnI G_GTAC'C Cuts at: 2722 2966 4484 2722 Size: 244 1518 5397 Fragments arranged by size: 5397 1518 244 MfeI C'AATT_G Cuts at: 4758 4758 Size: 7159 MluI A'CGCG_T Cuts at: 7035 7035 Size: 7159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4335 6273 6457 1305 Size: 3030 1938 184 2007 Fragments arranged by size: 3030 2007 1938 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2037 2909 2984 3182 4269 Size: 54 1425 343 205 872 75 198 1087 Cuts at: 4269 10 Size: 2900 Fragments arranged by size: 2900 1425 1087 872 343 205 198 75 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 5117 5476 5635 518 Size: 648 1362 2589 359 159 2042 Fragments arranged by size: 2589 2042 1362 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2155 2528 2714 2935 3835 3910 4198 4575 Size: 549 373 186 221 900 75 288 377 Cuts at: 4575 5252 6400 6645 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 900 677 549 377 373 288 245 221 186 75 NarI GG'CG_CC Cuts at: 2668 2668 Size: 7159 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5668 Fragments arranged by size: 5668 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7159 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7159 NotI GC'GGCC_GC Cuts at: 4606 4606 Size: 7159 NspI r_CATG'y Cuts at: 1132 1229 4517 6062 1132 Size: 97 3288 1545 2229 Fragments arranged by size: 3288 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2389 1348 Size: 1041 6118 PciI A'CATG_T Cuts at: 6058 6058 Size: 7159 PflMI CCAn_nnn'nTGG Cuts at: 1108 2638 3031 3325 1108 Size: 1530 393 294 4942 Fragments arranged by size: 4942 1530 393 294 PfoI T'CCnGG_A Cuts at: 2088 3549 3647 2088 Size: 1461 98 5600 Fragments arranged by size: 5600 1461 98 PmeI GTTT'AAAC Cuts at: 4463 4463 Size: 7159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5228 5260 6741 6773 5228 Size: 32 1481 32 5614 Fragments arranged by size: 5614 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 2577 3121 3142 3152 3711 2086 Size: 491 544 21 10 559 5534 Fragments arranged by size: 5534 559 544 491 21 10 PshAI GACnn'nnGTC Cuts at: 1613 2205 1613 Size: 592 6567 PsiI TTA'TAA Cuts at: 4729 4729 Size: 7159 PspOMI G'GGCC_C Cuts at: 4077 4077 Size: 7159 PstI C_TGCA'G Cuts at: 830 2808 2934 2952 3744 4056 4398 4511 830 Size: 1978 126 18 792 312 342 113 3478 Fragments arranged by size: 3478 1978 792 342 312 126 113 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7159 PvuII CAG'CTG Cuts at: 2155 2714 2935 3835 3910 2155 Size: 559 221 900 75 5404 Fragments arranged by size: 5404 900 559 221 75 SacI G_AGCT'C Cuts at: 2681 3507 4260 2681 Size: 826 753 5580 Fragments arranged by size: 5580 826 753 SalI G'TCGA_C Cuts at: 1936 2158 4501 1936 Size: 222 2343 4594 Fragments arranged by size: 4594 2343 222 SanDI GG'GwC_CC Cuts at: 3121 3121 Size: 7159 SapI GCTCTTCn'nnn_ Cuts at: 3512 3512 Size: 7159 SbfI CC_TGCA'GG Cuts at: 2952 4398 4511 2952 Size: 1446 113 5600 Fragments arranged by size: 5600 1446 113 ScaI AGT'ACT Cuts at: 5407 7119 5407 Size: 1712 5447 SfcI C'TryA_G Cuts at: 826 1045 2150 2804 2930 2948 3740 3850 4052 Size: 219 1105 654 126 18 792 110 202 Cuts at: 4052 4394 4507 5642 6323 6514 826 Size: 342 113 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 792 681 654 342 219 202 191 126 113 110 18 SfoI GGC'GCC Cuts at: 2669 2669 Size: 7159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7159 SmaI CCC'GGG Cuts at: 1872 3002 3170 4082 4100 4486 1872 Size: 1130 168 912 18 386 4545 Fragments arranged by size: 4545 1130 912 386 168 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 2810 3391 5263 6164 6426 820 Size: 197 926 867 581 1872 901 262 1553 Fragments arranged by size: 1872 1553 926 901 867 581 262 197 SnaBI TAC'GTA Cuts at: 493 4950 493 Size: 4457 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7159 SphI G_CATG'C Cuts at: 1132 4517 1132 Size: 3385 3774 SrfI GCCC'GGGC Cuts at: 3002 3002 Size: 7159 SspI AAT'ATT Cuts at: 5 52 5083 5 Size: 47 5031 2081 Fragments arranged by size: 5031 2081 47 StuI AGG'CCT Cuts at: 1517 2057 2228 2879 3731 4441 1517 Size: 540 171 651 852 710 4235 Fragments arranged by size: 4235 852 710 651 540 171 StyI C'CwwG_G Cuts at: 513 1065 2004 2910 3918 4133 4445 4598 513 Size: 552 939 906 1008 215 312 153 3074 Fragments arranged by size: 3074 1008 939 906 552 312 215 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5186 5203 5356 5541 5986 325 Size: 1255 3606 17 153 185 445 1498 Fragments arranged by size: 3606 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4068 5405 7117 Size: 274 80 33 51 1443 2091 1337 1712 Cuts at: 7117 96 Size: 138 Fragments arranged by size: 2091 1712 1443 1337 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2194 2226 3113 3145 3719 3751 3955 3987 7119 Size: 32 887 32 574 32 204 32 3132 Cuts at: 7119 7151 2194 Size: 32 2202 Fragments arranged by size: 3132 2202 887 574 204 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2294 1316 Size: 978 6181 XbaI T'CTAG_A Cuts at: 4429 4495 4429 Size: 66 7093 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3190 3274 3190 Size: 84 7075 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7159 XmaI C'CCGG_G Cuts at: 1870 3000 3168 4080 4098 4484 1870 Size: 1130 168 912 18 386 4545 Fragments arranged by size: 4545 1130 912 386 168 18 XmnI GAAnn'nnTTC Cuts at: 2372 5288 2372 Size: 2916 4243 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4967 276 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SwaI