################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbrPI BciVI BclI BfrI BlnI BlpI Bpu1102I BseAI BsiWI BspDI BspEI BspHI BspLU11I BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI HpaI KpnI MfeI MluI MluNI MroI MscI MunI NdeI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccI 1039 GTCTAC GT^[AC][GT]AC AlwNI 1609 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 985 GGATCC G^GATCC BbvCI 25 CCTCAGC CCTCAGC(-5/-2) BcgI 1859 GCACTCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 2368 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2540 ACCGGA [AT]^CCGG[AT] Bsp1407I 1283 TGTACA T^GTACA BsrDI 1146 CATTGC GCAATG(2/0) BsrGI 1283 TGTACA T^GTACA BstAPI 1210 GCACAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EcoRI 1330 GAATTC G^AATTC EcoRV 1927 GATATC GAT^ATC EcoT14I 824 CCATGG C^C[AT][AT]GG HincII 2810 GTCAAC GT[CT]^[GA]AC HindII 2810 GTCAAC GT[CT]^[GA]AC HindIII 2129 AAGCTT A^AGCTT KspI 212 CCGCGG CCGC^GG MamI 2368 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 824 CCATGG C^CATGG NheI 1219 GCTAGC G^CTAGC PvuII 1205 CAGCTG CAG^CTG SacII 212 CCGCGG CCGC^GG ScaI 1879 AGTACT AGT^ACT SgrAI 463 CGCCGGCG C[GA]^CCGG[CT]G SspBI 1283 TGTACA T^GTACA SspI 2271 AATATT AAT^ATT StyI 824 CCATGG C^C[AT][AT]GG XcmI 2769 CCAGATCAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### ApaI 480 GGGCCC GGGCC^C 1075 GGGCCC Asp700I 2483 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2509 GAAGAGTTTC BbsI 829 GAAGAC GAAGAC(2/6) 819 GTCTTC BglI 379 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 497 GCCCAGTCGGC BglII 2103 AGATCT A^GATCT 2229 AGATCT BpuAI 829 GAAGAC GAAGAC(2/6) 819 GTCTTC BsaAI 2726 TACGTG [CT]AC^GT[GA] 2899 CACGTA BsaI 2297 GAGACC GGTCTC(1/5) 595 GGTCTC BsmBI 2336 GAGACG CGTCTC(1/5) 2529 GAGACG BsmI 1487 GCATTC GAATGC(1/-1) 2550 GCATTC BspMI 938 ACCTGC ACCTGC(4/8) 2872 GCAGGT BsrBI 232 CCGCTC CCGCTC(-3/-3) 450 GAGCGG BsrFI 80 GCCGGC [GA]^CCGG[CT] 464 GCCGGC BssHII 65 GCGCGC G^CGCGC 404 GCGCGC Cfr10I 80 GCCGGC [GA]^CCGG[CT] 464 GCCGGC DraI 808 TTTAAA TTT^AAA 1377 TTTAAA DrdI 1761 GACAACCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2801 GACTAAATGGTC HgaI 40 GACGC GACGC(5/10) 2581 GACGC HphI 3 GGTGA GGTGA(8/7) 2526 GGTGA NaeI 80 GCCGGC GCC^GGC 464 GCCGGC NgoMIV 80 GCCGGC G^CCGGC 464 GCCGGC NotI 48 GCGGCCGC GC^GGCCGC 184 GCGGCCGC SacI 393 GAGCTC GAGCT^C 726 GAGCTC SapI 765 GCTCTTC GCTCTTC(1/4) 1716 GCTCTTC SmlI 1008 CTTGAG C^T[CT][GA]AG 1225 CTCAAG XmnI 2483 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2509 GAAGAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI