(Circular) (Six-base) MAPSORT of: pFN21AE3515.seq Check: 968 from: 1 to: 7609 pFN21AE3515 7609 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4868 4868 Size: 7609 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5419 278 Size: 53 83 186 936 3883 2468 Fragments arranged by size: 3883 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3045 4952 1937 Size: 1108 1907 4594 Fragments arranged by size: 4594 1907 1108 Acc65I G'GTAC_C Cuts at: 4930 4930 Size: 7609 AclI AA'CG_TT Cuts at: 1563 5736 6109 1563 Size: 4173 373 3063 Fragments arranged by size: 4173 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2928 3865 4080 4649 5672 7056 7324 1632 Size: 1296 937 215 569 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1296 1023 937 569 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7412 AflIII A'CryG_T Cuts at: 1203 6508 7485 1203 Size: 5305 977 1327 Fragments arranged by size: 5305 1327 977 AgeI A'CCGG_T Cuts at: 6440 6440 Size: 7609 AhdI GACnn_n'nnGTC Cuts at: 6338 6338 Size: 7609 AleI CACnn'nnGTG Cuts at: 1166 2786 4799 1166 Size: 1620 2013 3976 Fragments arranged by size: 3976 2013 1620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7609 AlwNI CAG_nnn'CTG Cuts at: 3619 5352 6924 3619 Size: 1733 1572 4304 Fragments arranged by size: 4304 1733 1572 ApaI G_GGCC'C Cuts at: 2489 3084 2489 Size: 595 7014 ApaLI G'TGCA_C Cuts at: 5666 6822 5666 Size: 1156 6453 ApoI r'AATT_y Cuts at: 1493 3335 3486 3728 4918 5144 5296 7405 1493 Size: 1842 151 242 1190 226 152 2109 1697 Fragments arranged by size: 2109 1842 1697 1190 242 226 152 151 AseI AT'TA_AT Cuts at: 160 1032 6163 160 Size: 872 5131 1606 Fragments arranged by size: 5131 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7609 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2080 2131 2362 2452 2559 4934 Size: 73 96 41 51 231 90 107 2375 Cuts at: 4934 1870 Size: 4545 Fragments arranged by size: 4545 2375 231 107 96 90 73 51 41 BamHI G'GATC_C Cuts at: 2990 4939 5306 2990 Size: 1949 367 5293 Fragments arranged by size: 5293 1949 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2358 2466 4930 6385 Size: 325 217 627 327 244 108 2464 1455 Cuts at: 6385 618 Size: 1842 Fragments arranged by size: 2464 1842 1455 627 327 325 244 217 108 BanII G_rGCy'C Cuts at: 1391 2402 2489 2735 3073 3084 4928 1391 Size: 1011 87 246 338 11 1844 4072 Fragments arranged by size: 4072 1844 1011 338 246 87 11 BbeI G_GCGC'C Cuts at: 2118 2362 2470 2118 Size: 244 108 7257 Fragments arranged by size: 7257 244 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2817 2841 928 Size: 971 918 24 5696 Fragments arranged by size: 5696 971 918 24 BbvCI CC'TCA_GC Cuts at: 2031 2031 Size: 7609 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3853 3887 5800 5834 1903 Size: 34 1916 34 1913 34 3678 Fragments arranged by size: 3678 1916 1913 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5501 6711 5501 Size: 1210 6399 BclI T'GATC_A Cuts at: 1548 1548 Size: 7609 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2390 2508 6220 Size: 107 122 71 631 605 718 118 3712 Cuts at: 6220 136 Size: 1525 Fragments arranged by size: 3712 1525 718 631 605 122 118 107 71 BglII A'GATC_T Cuts at: 4108 4234 7604 4108 Size: 126 3370 4113 Fragments arranged by size: 4113 3370 126 BlpI GC'TnA_GC Cuts at: 5026 5026 Size: 7609 Bme1580I G_kGCm'C Cuts at: 1790 2489 2498 3084 5670 6826 1790 Size: 699 9 586 2586 1156 2573 Fragments arranged by size: 2586 2573 1156 699 586 9 BmgBI CAC'GTC Cuts at: 1318 4397 1318 Size: 3079 4530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2498 3151 5340 Size: 416 53 83 399 24 1081 653 2189 Cuts at: 5340 6298 442 Size: 958 1753 Fragments arranged by size: 2189 1753 1081 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3228 1056 Size: 2172 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2180 4459 6269 1239 Size: 760 181 2279 1810 2579 Fragments arranged by size: 2579 2279 1810 760 181 Bpu10I CC'TnA_GC Cuts at: 2031 2031 Size: 7609 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3034 3215 5734 6599 6897 3034 Size: 181 2519 865 298 3746 Fragments arranged by size: 3746 2519 865 298 181 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2606 4296 6272 882 Size: 617 1107 1690 1976 2219 Fragments arranged by size: 2219 1976 1690 1107 617 BsaAI yAC'GTr Cuts at: 493 1204 4733 4906 5400 493 Size: 711 3529 173 494 2702 Fragments arranged by size: 3529 2702 711 494 173 BsaBI GATnn'nnATC Cuts at: 4377 7603 4377 Size: 3226 4383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2115 2359 2467 Size: 53 83 186 936 513 69 244 108 Cuts at: 2467 4587 5416 5798 6433 275 Size: 2120 829 382 635 1451 Fragments arranged by size: 2120 1451 936 829 635 513 382 244 186 108 83 69 53 BsaWI w'CCGG_w Cuts at: 4545 6042 6440 6714 6861 7272 4545 Size: 1497 398 274 147 411 4882 Fragments arranged by size: 4882 1497 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2958 2988 1178 Size: 30 165 30 1555 30 5799 Fragments arranged by size: 5799 1555 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2223 2309 2312 2315 2318 2321 2377 4215 Size: 1037 86 3 3 3 3 56 1838 Cuts at: 4215 4457 4648 1186 Size: 242 191 4147 Fragments arranged by size: 4147 1838 1037 242 191 86 56 3 3 3 3 BseYI C'CCAG_C Cuts at: 2498 2894 3340 4645 4684 4749 6812 7308 2498 Size: 396 446 1305 39 65 2063 496 2799 Fragments arranged by size: 2799 2063 1305 496 446 396 65 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 3198 4896 1163 Size: 573 309 1153 1698 3876 Fragments arranged by size: 3876 1698 1153 573 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2057 2109 2193 2211 2382 2394 Size: 275 186 56 52 84 18 171 12 Cuts at: 2394 5059 5820 6848 1540 Size: 2665 761 1028 2301 Fragments arranged by size: 2665 2301 1028 761 275 186 171 84 56 52 18 12 BsiHKAI G_wGCw'C Cuts at: 2252 2402 2735 5670 5755 6826 2252 Size: 150 333 2935 85 1071 3035 Fragments arranged by size: 3035 2935 1071 333 150 85 BsmI GAATG_Cn' Cuts at: 3492 4555 5120 5213 3492 Size: 1063 565 93 5888 Fragments arranged by size: 5888 1063 565 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4335 4528 4335 Size: 193 7416 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2252 2402 2489 2498 2735 3073 3084 Size: 399 462 150 87 9 237 338 11 Cuts at: 3084 4928 5670 5755 6826 1391 Size: 1844 742 85 1071 2174 Fragments arranged by size: 2174 1844 1071 742 462 399 338 237 150 87 85 11 9 BspEI T'CCGG_A Cuts at: 7272 7272 Size: 7609 BspHI T'CATG_A Cuts at: 5498 7228 5498 Size: 1730 5879 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2952 4868 4964 844 Size: 2108 1916 96 3489 Fragments arranged by size: 3489 2108 1916 96 BsrBI CCG'CTC Cuts at: 1394 1823 2239 2457 5496 1394 Size: 429 416 218 3039 3507 Fragments arranged by size: 3507 3039 429 416 218 BsrDI GCAATG_nn' Cuts at: 65 1248 3150 6104 6278 65 Size: 1183 1902 2954 174 1396 Fragments arranged by size: 2954 1902 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2469 6253 6440 1700 Size: 110 275 384 3784 187 2869 Fragments arranged by size: 3784 2869 384 275 187 110 BsrGI T'GTAC_A Cuts at: 96 3288 96 Size: 3192 4417 BssHII G'CGCG_C Cuts at: 1925 2070 2409 1925 Size: 145 339 7125 Fragments arranged by size: 7125 339 145 BssSI C'ACGA_G Cuts at: 5669 6681 5669 Size: 1012 6597 BstAPI GCAn_nnn'nTGC Cuts at: 794 3221 794 Size: 2427 5182 BstBI TT'CG_AA Cuts at: 6413 6413 Size: 7609 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7609 BstYI r'GATC_y Cuts at: 1972 2990 3102 4108 4234 4939 5306 5691 5708 Size: 1018 112 1006 126 705 367 385 17 Cuts at: 5708 7160 7246 7258 7604 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1018 1006 705 385 367 346 126 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 2217 2403 2437 2829 4167 Size: 552 939 105 108 186 34 392 1338 Cuts at: 4167 513 Size: 3955 Fragments arranged by size: 3955 1338 939 552 392 186 108 105 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2513 487 Size: 683 56 1287 5583 Fragments arranged by size: 5583 1287 683 56 BtsI GCAGTG_nn' Cuts at: 5125 5919 5939 5125 Size: 794 20 6795 Fragments arranged by size: 6795 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7574 DraI TTT'AAA Cuts at: 2815 3384 4913 2815 Size: 569 1529 5511 Fragments arranged by size: 5511 1529 569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3772 4812 6616 809 Size: 507 2456 1040 1804 1802 Fragments arranged by size: 2456 1804 1802 1040 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2106 2190 Size: 54 1425 50 275 18 224 52 84 Cuts at: 2190 2208 2379 2391 4668 5056 5945 7574 8 Size: 18 171 12 2277 388 889 1629 43 Fragments arranged by size: 2277 1629 1425 889 388 275 224 171 84 54 52 50 43 18 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2054 2106 2190 2208 2379 2391 5056 Size: 275 242 52 84 18 171 12 2665 Cuts at: 5056 1537 Size: 4090 Fragments arranged by size: 4090 2665 275 242 171 84 52 18 12 EarI CTCTTCn'nnn_ Cuts at: 1353 2777 3468 3728 4509 4625 4731 5539 1353 Size: 1424 691 260 781 116 106 808 3423 Fragments arranged by size: 3423 1424 808 781 691 260 116 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2343 2588 6196 6570 6716 387 Size: 1064 138 754 245 3608 374 146 1280 Fragments arranged by size: 3608 1280 1064 754 374 245 146 138 EcoICRI GAG'CTC Cuts at: 2400 2733 2400 Size: 333 7276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2180 2928 3865 4080 4459 4649 Size: 393 367 181 748 937 215 379 190 Cuts at: 4649 5672 6269 7056 7324 1239 Size: 1023 597 787 268 1524 Fragments arranged by size: 1524 1023 937 787 748 597 393 379 367 268 215 190 181 EcoO109I rG'GnC_Cy Cuts at: 2155 2200 2485 2518 3081 4366 2155 Size: 45 285 33 563 1285 5398 Fragments arranged by size: 5398 1285 563 285 45 33 EcoRI G'AATT_C Cuts at: 3335 4918 5296 3335 Size: 1583 378 5648 Fragments arranged by size: 5648 1583 378 EcoRV GAT'ATC Cuts at: 3934 3934 Size: 7609 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4231 4231 Size: 7609 FspI TGC'GCA Cuts at: 6115 6115 Size: 7609 HaeII r_GCGC'y Cuts at: 1678 2118 2362 2470 6756 1678 Size: 440 244 108 4286 2531 Fragments arranged by size: 4286 2531 440 244 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3032 3064 4040 4072 4221 Size: 32 135 32 1460 32 976 32 149 Cuts at: 4221 4253 6250 6282 6324 6356 1373 Size: 32 1997 32 42 32 2626 Fragments arranged by size: 2626 1997 1460 976 149 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4817 4953 5199 669 Size: 988 281 2879 136 246 3079 Fragments arranged by size: 3079 2879 988 281 246 136 HindIII A'AGCT_T Cuts at: 748 4134 748 Size: 3386 4223 HpaI GTT'AAC Cuts at: 5199 5199 Size: 7609 KasI G'GCGC_C Cuts at: 2114 2358 2466 2114 Size: 244 108 7257 Fragments arranged by size: 7257 244 108 KpnI G_GTAC'C Cuts at: 4934 4934 Size: 7609 MfeI C'AATT_G Cuts at: 5208 5208 Size: 7609 MluI A'CGCG_T Cuts at: 7485 7485 Size: 7609 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4722 6723 6907 1305 Size: 3333 84 2001 184 2007 Fragments arranged by size: 3333 2007 2001 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5787 Fragments arranged by size: 5787 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 2786 4257 4799 5567 5926 6085 Size: 648 1590 30 1471 542 768 359 159 Cuts at: 6085 518 Size: 2042 Fragments arranged by size: 2042 1590 1471 768 648 542 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2152 2219 2524 3212 5025 5702 6850 7095 Size: 546 67 305 688 1813 677 1148 245 Cuts at: 7095 1606 Size: 2120 Fragments arranged by size: 2120 1813 1148 688 677 546 305 245 67 NaeI GCC'GGC Cuts at: 2087 2471 2087 Size: 384 7225 NarI GG'CG_CC Cuts at: 2115 2359 2467 2115 Size: 244 108 7257 Fragments arranged by size: 7257 244 108 NcoI C'CATG_G Cuts at: 513 1065 2004 2829 513 Size: 552 939 825 5293 Fragments arranged by size: 5293 939 825 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7609 NgoMIV G'CCGG_C Cuts at: 2085 2469 2085 Size: 384 7225 NheI G'CTAG_C Cuts at: 1052 3224 1052 Size: 2172 5437 NotI GC'GGCC_GC Cuts at: 2054 2190 5056 2054 Size: 136 2866 4607 Fragments arranged by size: 4607 2866 136 NspI r_CATG'y Cuts at: 1132 1229 4967 6512 1132 Size: 97 3738 1545 2229 Fragments arranged by size: 3738 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7609 PciI A'CATG_T Cuts at: 6508 6508 Size: 7609 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7609 PmeI GTTT'AAAC Cuts at: 4913 4913 Size: 7609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5678 5710 7191 7223 5678 Size: 32 1481 32 6064 Fragments arranged by size: 6064 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2518 4366 2200 Size: 318 1848 5443 Fragments arranged by size: 5443 1848 318 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7609 PsiI TTA'TAA Cuts at: 5179 5179 Size: 7609 PspOMI G'GGCC_C Cuts at: 2485 3080 2485 Size: 595 7014 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4713 4745 4713 Size: 32 7577 PstI C_TGCA'G Cuts at: 830 4961 830 Size: 4131 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7609 PvuII CAG'CTG Cuts at: 3212 3212 Size: 7609 SacI G_AGCT'C Cuts at: 2402 2735 2402 Size: 333 7276 SacII CC_GC'GG Cuts at: 2220 2220 Size: 7609 SalI G'TCGA_C Cuts at: 1936 4951 1936 Size: 3015 4594 SanDI GG'GwC_CC Cuts at: 2200 2518 2200 Size: 318 7291 SapI GCTCTTCn'nnn_ Cuts at: 2777 3728 2777 Size: 951 6658 SbfI CC_TGCA'GG Cuts at: 4961 4961 Size: 7609 ScaI AGT'ACT Cuts at: 3886 5857 7569 3886 Size: 1971 1712 3926 Fragments arranged by size: 3926 1971 1712 SfcI C'TryA_G Cuts at: 826 1045 3567 3611 4857 4957 6092 6773 6964 Size: 219 2522 44 1246 100 1135 681 191 Cuts at: 6964 826 Size: 1471 Fragments arranged by size: 2522 1471 1246 1135 681 219 191 100 44 SfoI GGC'GCC Cuts at: 2116 2360 2468 2116 Size: 244 108 7257 Fragments arranged by size: 7257 244 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7609 SgrAI Cr'CCGG_yG Cuts at: 1700 2469 1700 Size: 769 6840 SmaI CCC'GGG Cuts at: 1872 2041 2082 2133 2364 4936 1872 Size: 169 41 51 231 2572 4545 Fragments arranged by size: 4545 2572 231 169 51 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 3013 3230 5713 6614 6876 820 Size: 197 926 1070 217 2483 901 262 1553 Fragments arranged by size: 2483 1553 1070 926 901 262 217 197 SnaBI TAC'GTA Cuts at: 493 5400 493 Size: 4907 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7609 SphI G_CATG'C Cuts at: 1132 4967 1132 Size: 3835 3774 SrfI GCCC'GGGC Cuts at: 2364 2364 Size: 7609 SspI AAT'ATT Cuts at: 5 52 4278 5533 5 Size: 47 4226 1255 2081 Fragments arranged by size: 4226 2081 1255 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7609 StyI C'CwwG_G Cuts at: 513 1065 2004 2829 5048 513 Size: 552 939 825 2219 3074 Fragments arranged by size: 3074 2219 939 825 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3267 4331 5636 5653 5806 5991 6436 Size: 1255 1687 1064 1305 17 153 185 445 Cuts at: 6436 325 Size: 1498 Fragments arranged by size: 1687 1498 1305 1255 1064 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 2877 3288 Size: 274 80 33 51 1443 765 135 411 Cuts at: 3288 3884 5855 7567 96 Size: 596 1971 1712 138 Fragments arranged by size: 1971 1712 1443 765 596 411 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2339 2354 2371 2757 2789 7569 7601 2322 Size: 17 15 17 386 32 4780 32 2330 Fragments arranged by size: 4780 2330 386 32 32 17 17 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7609 XbaI T'CTAG_A Cuts at: 4945 4945 Size: 7609 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4781 4781 Size: 7609 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7609 XmaI C'CCGG_G Cuts at: 1870 2039 2080 2131 2362 4934 1870 Size: 169 41 51 231 2572 4545 Fragments arranged by size: 4545 2572 231 169 51 41 XmnI GAAnn'nnTTC Cuts at: 4492 4518 5738 4492 Size: 26 1220 6363 Fragments arranged by size: 6363 1220 26 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5417 276 Size: 53 83 186 936 3883 2468 Fragments arranged by size: 3883 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SexAI SfiI SwaI