(Circular) (Six-base) MAPSORT of: pFN21AB5190.seq Check: 1583 from: 1 to: 6469 pFN21AB5190 6469 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3740 3740 Size: 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3812 1937 Size: 1875 4594 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 2826 3170 3432 3609 3701 4532 5916 Size: 855 339 344 262 177 92 831 1384 Cuts at: 5916 6184 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 855 831 344 339 268 262 177 92 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2422 1184 Size: 1238 5231 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3422 3454 3422 Size: 32 6437 AlwNI CAG_nnn'CTG Cuts at: 2396 4212 5784 2396 Size: 1816 1572 3081 Fragments arranged by size: 3081 1816 1572 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 2521 2655 3219 3778 4004 4156 6265 1493 Size: 1028 134 564 559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 564 559 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2789 3572 3794 1870 Size: 73 846 783 222 4545 Fragments arranged by size: 4545 846 783 222 73 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 3080 3122 3728 3790 Size: 325 217 627 1064 229 42 606 62 Cuts at: 3790 5245 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1064 627 606 325 229 217 62 42 BanII G_rGCy'C Cuts at: 1391 2548 3788 1391 Size: 1157 1240 4072 Fragments arranged by size: 4072 1240 1157 BbeI G_GCGC'C Cuts at: 3126 3126 Size: 6469 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2814 2934 3360 928 Size: 971 555 360 120 426 4037 Fragments arranged by size: 4037 971 555 426 360 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4660 4694 1903 Size: 34 50 34 2639 34 3678 Fragments arranged by size: 3678 2639 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2145 2707 4361 5571 2145 Size: 562 1654 1210 3043 Fragments arranged by size: 3043 1654 1210 562 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5080 136 Size: 107 122 71 631 605 3408 1525 Fragments arranged by size: 3408 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 2410 3886 2410 Size: 1476 4993 Bme1580I G_kGCm'C Cuts at: 1790 4530 5686 1790 Size: 2740 1156 2573 Fragments arranged by size: 2740 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2534 1318 Size: 1216 5253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2016 2384 4200 Size: 416 53 83 399 24 599 368 1816 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1816 1753 958 599 416 399 368 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2418 1056 Size: 1362 5107 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2755 2755 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2986 3215 3325 5129 1239 Size: 1747 229 110 1804 2579 Fragments arranged by size: 2579 1804 1747 229 110 Bpu10I CC'TnA_GC Cuts at: 3060 3060 Size: 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4594 5459 5757 4594 Size: 865 298 5306 Fragments arranged by size: 5306 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5132 882 Size: 617 3633 2219 Fragments arranged by size: 3633 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4260 493 Size: 711 3056 2702 Fragments arranged by size: 3056 2702 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3123 4276 4658 5293 Size: 53 83 186 936 1590 1153 382 635 Cuts at: 5293 275 Size: 1451 Fragments arranged by size: 1590 1451 1153 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4902 5300 5574 5721 6132 4902 Size: 398 274 147 411 5239 Fragments arranged by size: 5239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6244 Fragments arranged by size: 6244 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2053 2234 2237 2384 2839 1186 Size: 867 181 3 147 455 4816 Fragments arranged by size: 4816 867 455 181 147 3 BseYI C'CCAG_C Cuts at: 2231 2324 2537 3015 5672 6168 2231 Size: 93 213 478 2657 496 2532 Fragments arranged by size: 2657 2532 496 478 213 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2514 2610 1163 Size: 573 778 96 5022 Fragments arranged by size: 5022 778 573 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2249 3919 4680 5708 1540 Size: 275 186 47 201 1670 761 1028 2301 Fragments arranged by size: 2301 1670 1028 761 275 201 186 47 BsiHKAI G_wGCw'C Cuts at: 4530 4615 5686 4530 Size: 85 1071 5313 Fragments arranged by size: 5313 1071 85 BsmI GAATG_Cn' Cuts at: 3980 4073 3980 Size: 93 6376 BsmBI CGTCTCn'nnnn_ Cuts at: 2851 2851 Size: 6469 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2548 3788 4530 4615 5686 1391 Size: 399 758 1240 742 85 1071 2174 Fragments arranged by size: 2174 1240 1071 758 742 399 85 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2536 3740 3824 844 Size: 1692 1204 84 3489 Fragments arranged by size: 3489 1692 1204 84 BsrBI CCG'CTC Cuts at: 1394 1823 3043 4356 1394 Size: 429 1220 1313 3507 Fragments arranged by size: 3507 1313 1220 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3151 3486 4964 5138 65 Size: 1183 1903 335 1478 174 1396 Fragments arranged by size: 1903 1478 1396 1183 335 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5113 5300 1700 Size: 110 3303 187 2869 Fragments arranged by size: 3303 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 3281 4529 5541 3281 Size: 1248 1012 4209 Fragments arranged by size: 4209 1248 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6469 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstXI CCAn_nnnn'nTGG Cuts at: 2744 2744 Size: 6469 BstYI r'GATC_y Cuts at: 1972 3576 3799 4166 4551 4568 6020 6106 6118 Size: 1604 223 367 385 17 1452 86 12 Cuts at: 6118 6464 1972 Size: 346 1977 Fragments arranged by size: 1977 1604 1452 385 367 346 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2871 513 Size: 552 1806 4111 Fragments arranged by size: 4111 1806 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5730 Fragments arranged by size: 5730 683 56 BtsI GCAGTG_nn' Cuts at: 3262 3522 3985 4779 4799 3262 Size: 260 463 794 20 4932 Fragments arranged by size: 4932 794 463 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 2278 3773 2278 Size: 1495 4974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2874 3531 3916 Size: 54 1425 50 275 18 1044 657 385 Cuts at: 3916 4805 6434 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1044 889 657 385 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2164 2752 3033 3186 3189 3573 4399 1353 Size: 811 588 281 153 3 384 826 3423 Fragments arranged by size: 3423 826 811 588 384 281 153 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2127 5056 5430 5576 387 Size: 1064 138 538 2929 374 146 1280 Fragments arranged by size: 2929 1280 1064 538 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2826 2986 3170 3215 3325 3432 Size: 393 855 339 160 184 45 110 107 Cuts at: 3432 3609 3701 4532 5129 5916 6184 1239 Size: 177 92 831 597 787 268 1524 Fragments arranged by size: 1524 855 831 787 597 393 339 268 184 177 160 110 107 92 45 EcoNI CCTnn'n_nnAGG Cuts at: 3473 3473 Size: 6469 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 3498 4975 3498 Size: 1477 4992 HaeII r_GCGC'y Cuts at: 1678 3126 5616 1678 Size: 1448 2490 2531 Fragments arranged by size: 2531 2490 1448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2915 2944 2947 2976 3185 Size: 32 135 32 1343 29 3 29 209 Cuts at: 3185 3217 5110 5142 5184 5216 1373 Size: 32 1893 32 42 32 2626 Fragments arranged by size: 2626 1893 1343 209 135 42 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3813 4059 669 Size: 988 281 1875 246 3079 Fragments arranged by size: 3079 1875 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KasI G'GCGC_C Cuts at: 3122 3122 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2948 5583 5767 1305 Size: 1643 2635 184 2007 Fragments arranged by size: 2635 2007 1643 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3533 10 Size: 54 1425 343 1701 2946 Fragments arranged by size: 2946 1701 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2742 2901 3243 3449 4427 4786 4945 Size: 648 1576 159 342 206 978 359 159 Cuts at: 4945 518 Size: 2042 Fragments arranged by size: 2042 1576 978 648 359 342 206 159 159 MspA1I CmG'CkG Cuts at: 1606 3747 3885 4562 5710 5955 1606 Size: 2141 138 677 1148 245 2120 Fragments arranged by size: 2141 2120 1148 677 245 138 NarI GG'CG_CC Cuts at: 3123 3123 Size: 6469 NcoI C'CATG_G Cuts at: 513 1065 2871 513 Size: 552 1806 4111 Fragments arranged by size: 4111 1806 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 2414 1052 Size: 1362 5107 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 3754 3827 5372 1132 Size: 97 2525 73 1545 2229 Fragments arranged by size: 2525 2229 1545 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 3481 1108 Size: 2373 4096 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PstI C_TGCA'G Cuts at: 830 2401 3821 830 Size: 1571 1420 3478 Fragments arranged by size: 3478 1571 1420 PvuI CG_AT'CG Cuts at: 2001 2048 2001 Size: 47 6422 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SapI GCTCTTCn'nnn_ Cuts at: 3033 3186 3573 3033 Size: 153 387 5929 Fragments arranged by size: 5929 387 153 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 2698 4717 6429 2698 Size: 2019 1712 2738 Fragments arranged by size: 2738 2019 1712 SfcI C'TryA_G Cuts at: 826 1045 2397 3634 3817 4952 5633 5824 826 Size: 219 1352 1237 183 1135 681 191 1471 Fragments arranged by size: 1471 1352 1237 1135 681 219 191 183 SfoI GGC'GCC Cuts at: 3124 3124 Size: 6469 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4573 5474 5736 820 Size: 197 926 2630 901 262 1553 Fragments arranged by size: 2630 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 2110 4393 5 Size: 47 2058 2283 2081 Fragments arranged by size: 2283 2081 2058 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 2871 3224 3908 513 Size: 552 1806 353 684 3074 Fragments arranged by size: 3074 1806 684 552 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4496 4513 4666 4851 5296 325 Size: 1255 2916 17 153 185 445 1498 Fragments arranged by size: 2916 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2696 4715 Size: 274 80 33 51 1443 171 548 2019 Cuts at: 4715 6427 96 Size: 1712 138 Fragments arranged by size: 2019 1712 1443 548 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2684 2716 2884 2916 6429 6461 2684 Size: 32 168 32 3513 32 2692 Fragments arranged by size: 3513 2692 168 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2390 1316 Size: 1074 5395 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 3424 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6469 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 3168 4598 3168 Size: 1430 5039 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI