(Circular) (Six-base) MAPSORT of: pFN21AB6375.seq Check: 9908 from: 1 to: 5515 pFN21AB6375 5515 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2202 2202 Size: 5515 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 2407 4414 5391 1203 Size: 1204 2007 977 1327 Fragments arranged by size: 2007 1327 1204 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 3258 4830 3258 Size: 1572 3943 ApaI G_GGCC'C Cuts at: 2528 2528 Size: 5515 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2489 2824 3050 3202 5311 1493 Size: 996 335 226 152 2109 1697 Fragments arranged by size: 2109 1697 996 335 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2840 1870 Size: 73 897 4545 Fragments arranged by size: 4545 897 73 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2836 4291 618 Size: 325 217 627 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 627 325 217 BanII G_rGCy'C Cuts at: 1391 2145 2245 2528 2834 1391 Size: 754 100 283 306 4072 Fragments arranged by size: 4072 754 306 283 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2325 2484 928 Size: 971 426 159 3959 Fragments arranged by size: 3959 971 426 159 BbvCI CC'TCA_GC Cuts at: 2085 2085 Size: 5515 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4126 136 Size: 107 122 71 631 605 2454 1525 Fragments arranged by size: 2454 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2184 2356 5510 2184 Size: 172 3154 2189 Fragments arranged by size: 3154 2189 172 BlpI GC'TnA_GC Cuts at: 2453 2932 2453 Size: 479 5036 Bme1580I G_kGCm'C Cuts at: 1790 2528 3576 4732 1790 Size: 738 1048 1156 2573 Fragments arranged by size: 2573 1156 1048 738 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2783 3246 4204 Size: 416 53 83 399 24 1366 463 958 Cuts at: 4204 442 Size: 1753 Fragments arranged by size: 1753 1366 958 463 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 4175 1239 Size: 1169 1767 2579 Fragments arranged by size: 2579 1767 1169 Bpu10I CC'TnA_GC Cuts at: 2085 2085 Size: 5515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 3640 4505 4803 2000 Size: 1640 865 298 2712 Fragments arranged by size: 2712 1640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2410 3306 493 Size: 711 1206 896 2702 Fragments arranged by size: 2702 1206 896 711 BsaBI GATnn'nnATC Cuts at: 2055 5509 2055 Size: 3454 2061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2659 3322 3704 4339 Size: 53 83 186 936 1126 663 382 635 Cuts at: 4339 275 Size: 1451 Fragments arranged by size: 1451 1126 936 663 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5290 Fragments arranged by size: 5290 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5515 BseYI C'CCAG_C Cuts at: 2482 4718 5214 2482 Size: 2236 496 2783 Fragments arranged by size: 2783 2236 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4942 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2454 3576 3661 4732 2454 Size: 1122 85 1071 3237 Fragments arranged by size: 3237 1122 1071 85 BsmI GAATG_Cn' Cuts at: 2229 2275 3026 3119 2229 Size: 46 751 93 4625 Fragments arranged by size: 4625 751 93 46 BsmBI CGTCTCn'nnnn_ Cuts at: 2744 2744 Size: 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2245 2454 2528 2834 3576 3661 Size: 399 355 100 209 74 306 742 85 Cuts at: 3661 4732 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 355 306 209 100 85 74 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2870 844 Size: 1358 668 3489 Fragments arranged by size: 3489 1358 668 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2430 4010 4184 65 Size: 1183 1182 1580 174 1396 Fragments arranged by size: 1580 1396 1183 1182 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4159 4346 1700 Size: 110 2349 187 2869 Fragments arranged by size: 2869 2349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 2255 3575 4587 2255 Size: 1320 1012 3183 Fragments arranged by size: 3183 1320 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 2221 2734 4319 2221 Size: 513 1585 3417 Fragments arranged by size: 3417 1585 513 BstEII G'GTnAC_C Cuts at: 1184 2590 1184 Size: 1406 4109 BstYI r'GATC_y Cuts at: 1972 2184 2356 2845 3212 3597 3614 5066 5152 Size: 212 172 489 367 385 17 1452 86 Cuts at: 5152 5164 5510 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 489 385 367 346 212 172 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2394 2394 Size: 5515 BtgI C'CryG_G Cuts at: 513 1065 2004 2721 513 Size: 552 939 717 3307 Fragments arranged by size: 3307 939 717 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 3031 3825 3845 3031 Size: 794 20 4701 Fragments arranged by size: 4701 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2494 2819 2494 Size: 325 5190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480 Size: 54 1425 50 275 18 1132 889 1629 Cuts at: 5480 8 Size: 43 Fragments arranged by size: 1629 1425 1132 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2742 2750 3445 1353 Size: 1389 8 695 3423 Fragments arranged by size: 3423 1389 695 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 4476 4622 387 Size: 1064 138 2513 374 146 1280 Fragments arranged by size: 2513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 3578 4175 4962 5230 1239 Size: 393 776 1170 597 787 268 1524 Fragments arranged by size: 1524 1170 787 776 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2128 2516 2525 2614 2785 2128 Size: 388 9 89 171 4858 Fragments arranged by size: 4858 388 171 89 9 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 4662 1678 Size: 2984 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373 Size: 32 135 32 2584 32 42 32 2626 Fragments arranged by size: 2626 2584 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2713 2859 3105 669 Size: 988 281 775 146 246 3079 Fragments arranged by size: 3079 988 775 281 246 146 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 2530 3114 2530 Size: 584 4931 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3693 Fragments arranged by size: 3693 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2572 3473 3832 3991 518 Size: 648 1406 901 359 159 2042 Fragments arranged by size: 2042 1406 901 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2686 2764 2931 3608 4756 5001 1606 Size: 465 615 78 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 615 465 245 167 78 NcoI C'CATG_G Cuts at: 513 1065 2004 2721 513 Size: 552 939 717 3307 Fragments arranged by size: 3307 939 717 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2873 4418 1132 Size: 97 1644 1545 2229 Fragments arranged by size: 2229 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 1108 Size: 1252 4263 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PmlI CAC'GTG Cuts at: 2410 2410 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2516 2785 2128 Size: 388 269 4858 Fragments arranged by size: 4858 388 269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2524 2524 Size: 5515 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2281 2313 2281 Size: 32 5483 PstI C_TGCA'G Cuts at: 830 2199 2288 2622 2867 830 Size: 1369 89 334 245 3478 Fragments arranged by size: 3478 1369 334 245 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 PvuII CAG'CTG Cuts at: 2071 2686 2764 2071 Size: 615 78 4822 Fragments arranged by size: 4822 615 78 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2046 2195 2284 2618 2863 3998 4679 Size: 219 1001 149 89 334 245 1135 681 Cuts at: 4679 4870 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1001 681 334 245 219 191 149 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 3619 4520 4782 820 Size: 197 926 72 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 262 197 72 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5515 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 2721 2954 513 Size: 552 939 516 201 233 3074 Fragments arranged by size: 3074 939 552 516 233 201 SwaI ATTT'AAAT Cuts at: 2494 2494 Size: 5515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3559 3712 3897 4342 325 Size: 1255 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 2307 3761 Size: 274 80 33 51 1443 195 135 1454 Cuts at: 3761 5473 96 Size: 1712 138 Fragments arranged by size: 1712 1454 1443 274 195 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 2322 3644 2322 Size: 1322 4193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI