Restriction map for
pFN21AE0535
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# NO CUT ENZYMES ( 127 enzymes) #
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AccIII AclI AcsI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AvrII BalI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 6 GACGTC GACGT^C
Acc65I 236 GGTACC G^GTACC
AccI 415 GTCTAC GT^[AC][GT]AC
Alw44I 669 GTGCAC G^TGCAC
ApaI 657 GGGCCC GGGCC^C
ApaLI 669 GTGCAC G^TGCAC
Asp718I 236 GGTACC G^GTACC
AspI 816 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 734 CCCGGG C^[CT]CG[GA]G
AviII 404 TGCGCA TGC^GCA
BamHI 578 GGATCC G^GATCC
BbeI 830 GGCGCC GGCGC^C
BbvCI 492 CCTCAGC CCTCAGC(-5/-2)
BglI 393 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 734 CCCGGG C^[CT]CG[GA]G
BsrBI 907 GAGCGG CCGCTC(-3/-3)
BstYI 578 GGATCC [GA]^GATC[CT]
DraIII 550 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 892 CTCTTC CTCTTC(1/4)
EcoNI 375 CCTCAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 404 TGCGCA TGC^GCA
HaeII 830 GGCGCC [GA]GCGC^[CT]
KasI 830 GGCGCC G^GCGCC
KpnI 236 GGTACC GGTAC^C
Ksp632I 892 CTCTTC CTCTTC(1/4)
MflI 578 GGATCC [GA]^GATC[CT]
NarI 830 GGCGCC GG^CGCC
PflFI 816 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 891 GCTCTTC GCTCTTC(1/4)
SfoI 830 GGCGCC GGC^GCC
SgrAI 826 CACCGGCG C[GA]^CCGG[CT]G
SmaI 734 CCCGGG CCC^GGG
TaqI 1151 TCGA T^CGA
TfiI 291 GATTC G^A[AT]TC
Tsp509I 1171 AATT ^AATT
Tth111I 816 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1151 TCGA T^CGA
XhoII 578 GGATCC [GA]^GATC[CT]
XmaCI 734 CCCGGG C^CCGGG
XmaI 734 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BpmI 271 CTGGAG CTGGAG(16/14)
643 CTGGAG
Bsp1407I 329 TGTACA T^GTACA
425 TGTACA
BsrFI 239 ACCGGC [GA]^CCGG[CT]
827 ACCGGC
BsrGI 329 TGTACA T^GTACA
425 TGTACA
BstEII 630 GGTCACC G^GT[ACGT]ACC
729 GGTCACC
BstF5I 70 CATCC GGATG(2/0)
570 GGATG
BstPI 630 GGTCACC G^GT[ACGT]ACC
729 GGTCACC
BstXI 203 CCATTCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
968 CCACCAACGTGG
Cfr10I 239 ACCGGC [GA]^CCGG[CT]
827 ACCGGC
DdeI 91 CTGAG C^T[ACGT]AG
493 CTCAG
DsaI 11 CCGTGG C^C[GA][CT]GG
52 CCGTGG
EcoO65I 630 GGTCACC G^GT[ACGT]ACC
729 GGTCACC
EcoT14I 1024 CCAAGG C^C[AT][AT]GG
1160 CCAAGG
FokI 70 CATCC GGATG(9/13)
570 GGATG
PflMI 565 CCATCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
806 CCACCGACTGG
PleI 709 GACTC GAGTC(4/5)
646 GAGTC
SacI 748 GAGCTC GAGCT^C
889 GAGCTC
SfcI 103 CTACAG C^T[GA][CT]AG
417 CTACAG
SspBI 329 TGTACA T^GTACA
425 TGTACA
StyI 1024 CCAAGG C^C[AT][AT]GG
1160 CCAAGG
Van91I 565 CCATCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
806 CCACCGACTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI