(Circular) (Six-base) MAPSORT of: pFN21AB6340.seq Check: 2349 from: 1 to: 5575 pFN21AB6340 5575 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 3638 5022 5290 1632 Size: 617 1389 1384 268 1917 Fragments arranged by size: 1917 1389 1384 617 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 4474 5451 1203 Size: 3271 977 1327 Fragments arranged by size: 3271 1327 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AlwNI CAG_nnn'CTG Cuts at: 2572 2618 2758 3318 4890 2572 Size: 46 140 560 1572 3257 Fragments arranged by size: 3257 1572 560 140 46 ApaI G_GGCC'C Cuts at: 2750 2750 Size: 5575 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2884 3110 3262 5371 1493 Size: 1391 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 226 152 AseI AT'TA_AT Cuts at: 160 1032 2840 4129 160 Size: 872 1808 1289 1606 Fragments arranged by size: 1808 1606 1289 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2597 2900 1870 Size: 73 654 303 4545 Fragments arranged by size: 4545 654 303 73 BamHI G'GATC_C Cuts at: 2562 2905 3272 2562 Size: 343 367 4865 Fragments arranged by size: 4865 367 343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 2896 4351 618 Size: 325 217 627 1079 30 1455 1842 Fragments arranged by size: 1842 1455 1079 627 325 217 30 BanII G_rGCy'C Cuts at: 1391 2109 2427 2522 2701 2750 2894 1391 Size: 718 318 95 179 49 144 4072 Fragments arranged by size: 4072 718 318 179 144 95 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4604 BbvCI CC'TCA_GC Cuts at: 2154 2154 Size: 5575 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2540 2574 3766 3800 1903 Size: 34 603 34 1192 34 3678 Fragments arranged by size: 3678 1192 603 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 4693 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4186 136 Size: 107 122 71 631 605 2514 1525 Fragments arranged by size: 2514 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2036 2992 2036 Size: 956 4619 Bme1580I G_kGCm'C Cuts at: 1790 2750 3636 4792 1790 Size: 960 886 1156 2573 Fragments arranged by size: 2573 1156 960 886 BmgBI CAC'GTC Cuts at: 1318 2245 1318 Size: 927 4648 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3306 4264 442 Size: 416 53 83 399 24 1889 958 1753 Fragments arranged by size: 1889 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2093 2735 4235 1239 Size: 812 42 642 1500 2579 Fragments arranged by size: 2579 1500 812 642 42 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2474 3700 4565 4863 2474 Size: 1226 865 298 3186 Fragments arranged by size: 3186 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4238 882 Size: 617 2739 2219 Fragments arranged by size: 2739 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3366 493 Size: 711 2162 2702 Fragments arranged by size: 2702 2162 711 BsaBI GATnn'nnATC Cuts at: 2435 5569 2435 Size: 3134 2441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3382 3764 4399 275 Size: 53 83 186 936 1849 382 635 1451 Fragments arranged by size: 1849 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2391 2482 2823 4008 4406 4680 4827 5238 2391 Size: 91 341 1185 398 274 147 411 2728 Fragments arranged by size: 2728 1185 411 398 341 274 147 91 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5350 Fragments arranged by size: 5350 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2173 2223 2267 2270 2291 2651 1186 Size: 907 80 50 44 3 21 360 4110 Fragments arranged by size: 4110 907 360 80 50 44 21 3 BseYI C'CCAG_C Cuts at: 2023 2288 2572 2751 4778 5274 2023 Size: 265 284 179 2027 496 2324 Fragments arranged by size: 2324 2027 496 284 265 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5002 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 3025 3786 4814 1540 Size: 275 186 54 970 761 1028 2301 Fragments arranged by size: 2301 1028 970 761 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2109 2427 3636 3721 4792 2109 Size: 318 1209 85 1071 2892 Fragments arranged by size: 2892 1209 1071 318 85 BsmI GAATG_Cn' Cuts at: 3086 3179 3086 Size: 93 5482 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2427 2522 2701 2750 2894 3636 Size: 399 319 318 95 179 49 144 742 Cuts at: 3636 3721 4792 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 318 179 144 95 85 49 BspEI T'CCGG_A Cuts at: 2482 5238 2482 Size: 2756 2819 BspHI T'CATG_A Cuts at: 2800 3464 5194 2800 Size: 664 1730 3181 Fragments arranged by size: 3181 1730 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2544 3462 1394 Size: 429 721 918 3507 Fragments arranged by size: 3507 918 721 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2505 2863 4219 4406 1700 Size: 110 695 358 1356 187 2869 Fragments arranged by size: 2869 1356 695 358 187 110 BsrGI T'GTAC_A Cuts at: 96 2583 96 Size: 2487 3088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 2426 3635 4647 2426 Size: 1209 1012 3354 Fragments arranged by size: 3354 1209 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2311 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2562 2905 3272 3657 3674 5126 5212 5224 Size: 590 343 367 385 17 1452 86 12 Cuts at: 5224 5570 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 590 385 367 346 343 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2422 487 Size: 683 56 1196 3640 Fragments arranged by size: 3640 1196 683 56 BtsI GCAGTG_nn' Cuts at: 3091 3885 3905 3091 Size: 794 20 4761 Fragments arranged by size: 4761 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2372 4582 809 Size: 507 1056 2210 1802 Fragments arranged by size: 2210 1802 1056 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2507 2667 2847 Size: 54 1425 50 275 18 677 160 180 Cuts at: 2847 3022 3911 5540 8 Size: 175 889 1629 43 Fragments arranged by size: 1629 1425 889 677 275 180 175 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 3505 1353 Size: 1469 683 3423 Fragments arranged by size: 3423 1469 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2060 4162 4536 4682 387 Size: 1064 138 471 2102 374 146 1280 Fragments arranged by size: 2102 1280 1064 471 374 146 138 EcoICRI GAG'CTC Cuts at: 2107 2425 2107 Size: 318 5257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2093 2249 2735 3638 4235 5022 Size: 393 419 42 156 486 903 597 787 Cuts at: 5022 5290 1239 Size: 268 1524 Fragments arranged by size: 1524 903 787 597 486 419 393 268 156 42 EcoO109I rG'GnC_Cy Cuts at: 2449 2578 2615 2746 2747 2449 Size: 129 37 131 1 5277 Fragments arranged by size: 5277 131 129 37 1 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2833 2833 Size: 5575 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 4722 1678 Size: 3044 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2782 2814 4216 4248 4290 Size: 32 135 32 1210 32 1402 32 42 Cuts at: 4290 4322 1373 Size: 32 2626 Fragments arranged by size: 2626 1402 1210 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2377 2919 3165 669 Size: 988 281 439 542 246 3079 Fragments arranged by size: 3079 988 542 439 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4689 4873 1305 Size: 3384 184 2007 Fragments arranged by size: 3384 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2849 10 Size: 54 1425 343 1017 2736 Fragments arranged by size: 2736 1425 1017 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 3533 3892 4051 518 Size: 648 1137 1230 359 159 2042 Fragments arranged by size: 2042 1230 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2476 2758 2785 2991 3668 4816 5061 1606 Size: 870 282 27 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 677 282 245 206 27 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2933 4478 1132 Size: 97 1704 1545 2229 Fragments arranged by size: 2229 1704 1545 97 PasI CC'CwG_GG Cuts at: 1348 2701 1348 Size: 1353 4222 PciI A'CATG_T Cuts at: 4474 4474 Size: 5575 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 1108 Size: 1464 4111 PfoI T'CCnGG_A Cuts at: 2565 2565 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 3644 3676 5157 5189 2528 Size: 32 1084 32 1481 32 2914 Fragments arranged by size: 2914 1481 1084 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 2578 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PspOMI G'GGCC_C Cuts at: 2746 2746 Size: 5575 PstI C_TGCA'G Cuts at: 830 2094 2790 2927 830 Size: 1264 696 137 3478 Fragments arranged by size: 3478 1264 696 137 PvuI CG_AT'CG Cuts at: 2001 2055 2001 Size: 54 5521 PvuII CAG'CTG Cuts at: 2476 2758 2476 Size: 282 5293 SacI G_AGCT'C Cuts at: 2109 2427 2109 Size: 318 5257 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2090 2786 2923 4058 4739 4930 826 Size: 219 1045 696 137 1135 681 191 1471 Fragments arranged by size: 1471 1135 1045 696 681 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2599 2902 1872 Size: 727 303 4545 Fragments arranged by size: 4545 727 303 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 3679 4580 4842 820 Size: 197 926 510 1226 901 262 1553 Fragments arranged by size: 1553 1226 926 901 510 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 2096 1517 Size: 579 4996 StyI C'CwwG_G Cuts at: 513 1065 2004 3014 513 Size: 552 939 1010 3074 Fragments arranged by size: 3074 1010 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2264 3602 3619 3772 3957 4402 325 Size: 1255 684 1338 17 153 185 445 1498 Fragments arranged by size: 1498 1338 1255 684 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2583 3821 5533 Size: 274 80 33 51 1443 606 1238 1712 Cuts at: 5533 96 Size: 138 Fragments arranged by size: 1712 1443 1238 606 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5535 5567 5535 Size: 32 5543 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2767 2911 2767 Size: 144 5431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2156 2156 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2597 2900 1870 Size: 727 303 4545 Fragments arranged by size: 4545 727 303 XmnI GAAnn'nnTTC Cuts at: 2198 3704 2198 Size: 1506 4069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI