Restriction map for
pFN21AB8896
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspI StuI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 580 GGTACC G^GTACC
AcsI 373 GAATTT [GA]^AATT[CT]
AflIII 530 ACATGT A^C[GA][CT]GT
Alw44I 331 GTGCAC G^TGCAC
AlwNI 613 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 331 GTGCAC G^TGCAC
ApoI 373 GAATTT [GA]^AATT[CT]
Asp718I 580 GGTACC G^GTACC
AspHI 331 GTGCAC G[AT]GC[AT]^C
BanI 580 GGTACC G^G[CT][GA]CC
BbsI 284 GAAGAC GAAGAC(2/6)
BcnI 321 CCCGG CC^[GC]GG
BlpI 436 GCTCAGC GC^T[ACGT]AGC
BmyI 331 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 436 GCTCAGC GC^T[ACGT]AGC
BpuAI 284 GAAGAC GAAGAC(2/6)
BsiHKAI 331 GTGCAC G[AT]GC[AT]^C
Bsp1286I 331 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 527 TGTACA T^GTACA
BspLU11I 530 ACATGT A^CATGT
BsrGI 527 TGTACA T^GTACA
BssSI 188 CTCGTG CACGAG(-5/-1)
BstF5I 299 CATCC GGATG(2/0)
BstYI 20 GGATCT [GA]^GATC[CT]
Cac8I 612 GCAGGC GC[ACGT]^[ACGT]GC
CelII 436 GCTCAGC GC^T[ACGT]AGC
EarI 202 CTCTTC CTCTTC(1/4)
EcoNI 453 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 299 CATCC GGATG(9/13)
HapII 322 CCGG C^CGG
HpaII 322 CCGG C^CGG
KpnI 580 GGTACC GGTAC^C
Ksp632I 202 CTCTTC CTCTTC(1/4)
MflI 20 GGATCT [GA]^GATC[CT]
MspI 322 CCGG C^CGG
MwoI 132 GCTGGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 321 CCCGG CC^[GC]GG
NspI 530 ACATGT [GA]CATG^[CT]
SpeI 240 ACTAGT A^CTAGT
SspBI 527 TGTACA T^GTACA
Tsp509I 374 AATT ^AATT
XcmI 443 CCAAGATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 20 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 10 CCGC CCGC(-3/-1)
130 CCGC
AfaI 528 GTAC GT^AC
581 GTAC
AlwI 20 GGATC GGATC(4/5)
380 GGATC
BbvI 12 GCTGC GCAGC(8/12)
546 GCTGC
BclI 108 TGATCA T^GATCA
122 TGATCA
BfaI 92 CTAG C^TAG
241 CTAG
BpmI 326 CTCCAG CTGGAG(16/14)
133 CTGGAG
BsmFI 388 GGGAC GGGAC(10/14)
536 GGGAC
BstXI 35 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
596 CCAATATCCTGG
DdeI 67 CTCAG C^T[ACGT]AG
437 CTCAG
EcoT14I 219 CCAAGG C^C[AT][AT]GG
656 CCTTGG
FbaI 108 TGATCA T^GATCA
122 TGATCA
HphI 399 GGTGA GGTGA(8/7)
32 TCACC
MaeI 92 CTAG C^TAG
241 CTAG
MboII 284 GAAGA GAAGA(8/7)
203 TCTTC
MslI 334 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
336 CATCTGTGTG
MspA1I 10 CCGCTG C[AC]G^C[GT]G
130 CCGCTG
NlaIII 112 CATG CATG^
531 CATG
PleI 538 GACTC GAGTC(4/5)
352 GAGTC
RsaI 528 GTAC GT^AC
581 GTAC
StyI 219 CCAAGG C^C[AT][AT]GG
656 CCTTGG
TaqI 168 TCGA T^CGA
311 TCGA
TfiI 97 GAATC G^A[AT]TC
469 GATTC
TseI 12 GCTGC G^C[AT]GC
546 GCTGC
TspRI 69 CAGTG CA[GC]TG[ACGT][ACGT]^
329 CAGTG
TthHB8I 168 TCGA T^CGA
311 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsrI
BssKI BstNI DpnII EcoRII Fnu4HI
HinfI ItaI MaeIII MboI MnlI
MvaI NdeII NlaIV Sau3AI ScrFI
SfcI