(Circular) (Six-base) MAPSORT of: pFN21AB8896.seq Check: 6565 from: 1 to: 5374 pFN21AB8896 5374 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2585 2695 2585 Size: 110 5264 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 2535 4273 5250 1203 Size: 1332 1738 977 1327 Fragments arranged by size: 1738 1332 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 2343 1166 Size: 1177 4197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 2623 3117 4689 2623 Size: 494 1572 3308 Fragments arranged by size: 3308 1572 494 ApaLI G'TGCA_C Cuts at: 2336 3431 4587 2336 Size: 1095 1156 3123 Fragments arranged by size: 3123 1156 1095 ApoI r'AATT_y Cuts at: 1493 2378 2683 2909 3061 5170 1493 Size: 885 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2699 1870 Size: 73 756 4545 Fragments arranged by size: 4545 756 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2585 2695 4150 618 Size: 325 217 627 798 110 1455 1842 Fragments arranged by size: 1842 1455 798 627 325 217 110 BanII G_rGCy'C Cuts at: 1391 2693 1391 Size: 1302 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2296 928 Size: 971 397 4006 Fragments arranged by size: 4006 971 397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 2113 2127 1548 Size: 565 14 4795 Fragments arranged by size: 4795 565 14 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3985 136 Size: 107 122 71 631 605 2313 1525 Fragments arranged by size: 2313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2442 2791 2442 Size: 349 5025 Bme1580I G_kGCm'C Cuts at: 1790 2340 3435 4591 1790 Size: 550 1095 1156 2573 Fragments arranged by size: 2573 1156 1095 550 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2316 4034 1239 Size: 920 157 1718 2579 Fragments arranged by size: 2579 1718 920 157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3181 3563 4198 275 Size: 53 83 186 936 1648 382 635 1451 Fragments arranged by size: 1648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5374 BseYI C'CCAG_C Cuts at: 2352 4577 5073 2352 Size: 2225 496 2653 Fragments arranged by size: 2653 2225 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4801 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 3435 3520 4591 2340 Size: 1095 85 1071 3123 Fragments arranged by size: 3123 1095 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 2693 3435 3520 4591 1391 Size: 399 550 353 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 399 353 85 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 2532 96 Size: 2436 2938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 2193 3434 4446 2193 Size: 1241 1012 3121 Fragments arranged by size: 3121 1241 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2608 2047 Size: 561 4813 BstYI r'GATC_y Cuts at: 1972 2025 2704 3071 3456 3473 4925 5011 5023 Size: 53 679 367 385 17 1452 86 12 Cuts at: 5023 5369 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 679 385 367 346 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4822 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3710 5339 Size: 54 1425 50 275 18 991 889 1629 Cuts at: 5339 8 Size: 43 Fragments arranged by size: 1629 1425 991 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 3304 1353 Size: 860 1091 3423 Fragments arranged by size: 3423 1091 860 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2316 3437 4034 4821 5089 1239 Size: 393 527 157 1121 597 787 268 1524 Fragments arranged by size: 1524 1121 787 597 527 393 268 157 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 5374 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 4521 1678 Size: 2843 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 2631 2634 2663 Size: 32 135 32 776 32 251 3 29 Cuts at: 2663 2666 4015 4047 4089 4121 1373 Size: 3 1349 32 42 32 2626 Fragments arranged by size: 2626 1349 776 251 135 42 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2589 2699 2589 Size: 110 5264 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2245 4488 4672 1305 Size: 940 2243 184 2007 Fragments arranged by size: 2243 2007 940 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3552 Fragments arranged by size: 3552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 2345 3332 3691 3850 518 Size: 648 1177 2 987 359 159 2042 Fragments arranged by size: 2042 1177 987 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2017 2137 2790 3467 4615 4860 1606 Size: 411 120 653 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 653 411 245 120 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2539 2732 4277 1132 Size: 97 1310 193 1545 2229 Fragments arranged by size: 2229 1545 1310 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 2535 4273 2535 Size: 1738 3636 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PfoI T'CCnGG_A Cuts at: 2046 2607 2046 Size: 561 4813 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2253 2944 2253 Size: 691 4683 PstI C_TGCA'G Cuts at: 830 2726 830 Size: 1896 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2150 2179 2200 2722 3857 4538 4729 Size: 219 1105 29 21 522 1135 681 191 Cuts at: 4729 826 Size: 1471 Fragments arranged by size: 1471 1135 1105 681 522 219 191 29 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 2245 152 Size: 2093 3281 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2224 2661 2813 513 Size: 552 1159 437 152 3074 Fragments arranged by size: 3074 1159 552 437 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 3620 5332 Size: 274 80 33 51 1443 555 1088 1712 Cuts at: 5332 96 Size: 138 Fragments arranged by size: 1712 1443 1088 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5334 5366 5334 Size: 32 5342 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2455 2455 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI