(Circular) (Six-base) MAPSORT of: pFN21AB8417.seq Check: 6408 from: 1 to: 7060 pFN21AB8417 7060 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4870 278 Size: 53 83 186 936 3334 2468 Fragments arranged by size: 3334 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4403 1937 Size: 2466 4594 Acc65I G'GTAC_C Cuts at: 4381 4381 Size: 7060 AclI AA'CG_TT Cuts at: 1563 5187 5560 1563 Size: 3624 373 3063 Fragments arranged by size: 3624 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2728 3087 3588 5123 6507 6775 1632 Size: 1096 359 501 1535 1384 268 1917 Fragments arranged by size: 1917 1535 1384 1096 501 359 268 AflII C'TTAA_G Cuts at: 820 1017 3872 820 Size: 197 2855 4008 Fragments arranged by size: 4008 2855 197 AflIII A'CryG_T Cuts at: 1203 2251 3432 5959 6936 1203 Size: 1048 1181 2527 977 1327 Fragments arranged by size: 2527 1327 1181 1048 977 AgeI A'CCGG_T Cuts at: 5891 5891 Size: 7060 AhdI GACnn_n'nnGTC Cuts at: 5789 5789 Size: 7060 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7060 AlwNI CAG_nnn'CTG Cuts at: 3203 4803 6375 3203 Size: 1600 1572 3888 Fragments arranged by size: 3888 1600 1572 ApaLI G'TGCA_C Cuts at: 5117 6273 5117 Size: 1156 5904 ApoI r'AATT_y Cuts at: 1493 2797 4369 4595 4747 6856 1493 Size: 1304 1572 226 152 2109 1697 Fragments arranged by size: 2109 1697 1572 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 3297 5614 160 Size: 872 2265 2317 1606 Fragments arranged by size: 2317 2265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 AvaI C'yCGr_G Cuts at: 1870 1943 4385 1870 Size: 73 2442 4545 Fragments arranged by size: 4545 2442 73 BamHI G'GATC_C Cuts at: 4390 4757 4390 Size: 367 6693 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 4381 5836 618 Size: 325 217 627 221 2373 1455 1842 Fragments arranged by size: 2373 1842 1455 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2136 3796 4379 1391 Size: 745 1660 583 4072 Fragments arranged by size: 4072 1660 745 583 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 7060 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2734 2861 3234 928 Size: 971 835 127 373 4754 Fragments arranged by size: 4754 971 835 373 127 BbvCI CC'TCA_GC Cuts at: 3888 3888 Size: 7060 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5251 5285 1903 Size: 34 3314 34 3678 Fragments arranged by size: 3678 3314 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2848 3026 3491 4952 6162 2848 Size: 178 465 1461 1210 3746 Fragments arranged by size: 3746 1461 1210 465 178 BclI T'GATC_A Cuts at: 1548 2164 1548 Size: 616 6444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2524 5671 136 Size: 107 122 71 631 605 852 3147 1525 Fragments arranged by size: 3147 1525 852 631 605 122 107 71 BglII A'GATC_T Cuts at: 2749 3981 7055 2749 Size: 1232 3074 2754 Fragments arranged by size: 3074 2754 1232 BlpI GC'TnA_GC Cuts at: 4477 4477 Size: 7060 Bme1580I G_kGCm'C Cuts at: 1790 3961 5121 6277 1790 Size: 2171 1160 1156 2573 Fragments arranged by size: 2573 2171 1160 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2228 3542 4791 Size: 416 53 83 399 24 811 1314 1249 Cuts at: 4791 5749 442 Size: 958 1753 Fragments arranged by size: 1753 1314 1249 958 811 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7060 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2074 2074 Size: 7060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2992 5720 1239 Size: 1753 2728 2579 Fragments arranged by size: 2728 2579 1753 Bpu10I CC'TnA_GC Cuts at: 2225 3888 2225 Size: 1663 5397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 3549 5185 6050 6348 2105 Size: 1444 1636 865 298 2817 Fragments arranged by size: 2817 1636 1444 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5723 882 Size: 617 4224 2219 Fragments arranged by size: 4224 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4851 493 Size: 711 3647 2702 Fragments arranged by size: 3647 2702 711 BsaBI GATnn'nnATC Cuts at: 2277 7054 2277 Size: 4777 2283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3126 4867 5249 Size: 53 83 186 936 476 1117 1741 382 Cuts at: 5249 5884 275 Size: 635 1451 Fragments arranged by size: 1741 1451 1117 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 3819 5493 5891 6165 6312 6723 2382 Size: 1437 1674 398 274 147 411 2719 Fragments arranged by size: 2719 1674 1437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4029 4059 1178 Size: 30 165 30 2626 30 4179 Fragments arranged by size: 4179 2626 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2577 1186 Size: 1391 5669 BseYI C'CCAG_C Cuts at: 3960 6263 6759 3960 Size: 2303 496 4261 Fragments arranged by size: 4261 2303 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3264 1163 Size: 573 1528 4959 Fragments arranged by size: 4959 1528 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4510 5271 6299 1540 Size: 275 186 2509 761 1028 2301 Fragments arranged by size: 2509 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5121 5206 6277 5121 Size: 85 1071 5904 Fragments arranged by size: 5904 1071 85 BsmI GAATG_Cn' Cuts at: 3254 4571 4664 3254 Size: 1317 93 5650 Fragments arranged by size: 5650 1317 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 3796 3961 4379 5121 5206 6277 Size: 399 346 1660 165 418 742 85 1071 Cuts at: 6277 1391 Size: 2174 Fragments arranged by size: 2174 1660 1071 742 418 399 346 165 85 BspEI T'CCGG_A Cuts at: 6723 6723 Size: 7060 BspHI T'CATG_A Cuts at: 4949 6679 4949 Size: 1730 5330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4415 844 Size: 3571 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4947 1394 Size: 429 3124 3507 Fragments arranged by size: 3507 3124 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2551 5555 5729 65 Size: 1183 1303 3004 174 1396 Fragments arranged by size: 3004 1396 1303 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5704 5891 1700 Size: 110 3894 187 2869 Fragments arranged by size: 3894 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3973 96 Size: 3877 3183 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7060 BssSI C'ACGA_G Cuts at: 3977 5120 6132 3977 Size: 1143 1012 4905 Fragments arranged by size: 4905 1143 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 3599 794 Size: 1301 1504 4255 Fragments arranged by size: 4255 1504 1301 BstBI TT'CG_AA Cuts at: 5864 5864 Size: 7060 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7060 BstXI CCAn_nnnn'nTGG Cuts at: 3509 3509 Size: 7060 BstYI r'GATC_y Cuts at: 1972 2749 3981 4390 4757 5142 5159 6611 6697 Size: 777 1232 409 367 385 17 1452 86 Cuts at: 6697 6709 7055 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1232 777 409 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2707 513 Size: 552 939 703 4866 Fragments arranged by size: 4866 939 703 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6321 Fragments arranged by size: 6321 683 56 BtsI GCAGTG_nn' Cuts at: 4576 5370 5390 4576 Size: 794 20 6246 Fragments arranged by size: 6246 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7060 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7025 DraI TTT'AAA Cuts at: 4191 4338 4364 4191 Size: 147 26 6887 Fragments arranged by size: 6887 147 26 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6067 809 Size: 507 4751 1802 Fragments arranged by size: 4751 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 4507 5396 Size: 54 1425 50 275 18 234 2443 889 Cuts at: 5396 7025 8 Size: 1629 43 Fragments arranged by size: 2443 1629 1425 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4507 1537 Size: 275 2695 4090 Fragments arranged by size: 4090 2695 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 2916 4990 1353 Size: 788 775 2074 3423 Fragments arranged by size: 3423 2074 788 775 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5647 6021 6167 387 Size: 1064 138 4058 374 146 1280 Fragments arranged by size: 4058 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2728 2992 3087 3588 5123 5720 6507 Size: 393 1096 264 95 501 1535 597 787 Cuts at: 6507 6775 1239 Size: 268 1524 Fragments arranged by size: 1535 1524 1096 787 597 501 393 268 264 95 EcoNI CCTnn'n_nnAGG Cuts at: 3027 3027 Size: 7060 EcoO109I rG'GnC_Cy Cuts at: 3020 3884 3020 Size: 864 6196 EcoRI G'AATT_C Cuts at: 2797 4369 4747 2797 Size: 1572 378 5110 Fragments arranged by size: 5110 1572 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2431 3092 2431 Size: 661 6399 FspI TGC'GCA Cuts at: 5566 5566 Size: 7060 HaeII r_GCGC'y Cuts at: 1678 2012 6207 1678 Size: 334 4195 2531 Fragments arranged by size: 4195 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2941 2973 2990 3022 5701 Size: 32 135 32 1369 32 17 32 2679 Cuts at: 5701 5733 5775 5807 1373 Size: 32 42 32 2626 Fragments arranged by size: 2679 2626 1369 135 42 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 4035 4404 4650 669 Size: 988 281 2097 369 246 3079 Fragments arranged by size: 3079 2097 988 369 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7060 HpaI GTT'AAC Cuts at: 4035 4650 4035 Size: 615 6445 KasI G'GCGC_C Cuts at: 2008 2008 Size: 7060 KpnI G_GTAC'C Cuts at: 4385 4385 Size: 7060 MfeI C'AATT_G Cuts at: 2802 4003 4659 2802 Size: 1201 656 5203 Fragments arranged by size: 5203 1201 656 MluI A'CGCG_T Cuts at: 6936 6936 Size: 7060 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3580 4200 6174 6358 1305 Size: 2275 620 1974 184 2007 Fragments arranged by size: 2275 2007 1974 620 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 10 Size: 54 1425 343 234 5004 Fragments arranged by size: 5004 1425 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 5018 5377 5536 518 Size: 648 3852 359 159 2042 Fragments arranged by size: 3852 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4261 4298 4476 5153 6301 6546 1606 Size: 2655 37 178 677 1148 245 2120 Fragments arranged by size: 2655 2120 1148 677 245 178 37 NarI GG'CG_CC Cuts at: 2009 2009 Size: 7060 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5569 Fragments arranged by size: 5569 939 552 NdeI CA'TA_TG Cuts at: 387 2161 387 Size: 1774 5286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7060 NotI GC'GGCC_GC Cuts at: 4507 4507 Size: 7060 NspI r_CATG'y Cuts at: 1132 1229 2255 2762 3436 4418 5963 1132 Size: 97 1026 507 674 982 1545 2229 Fragments arranged by size: 2229 1545 1026 982 674 507 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7060 PciI A'CATG_T Cuts at: 2251 3432 5959 2251 Size: 1181 2527 3352 Fragments arranged by size: 3352 2527 1181 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7060 PmeI GTTT'AAAC Cuts at: 4364 4364 Size: 7060 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5129 5161 6642 6674 5129 Size: 32 1481 32 5515 Fragments arranged by size: 5515 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3020 3884 3020 Size: 864 6196 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7060 PsiI TTA'TAA Cuts at: 3102 3665 3738 4269 4630 3102 Size: 563 73 531 361 5532 Fragments arranged by size: 5532 563 531 361 73 PstI C_TGCA'G Cuts at: 830 4412 830 Size: 3582 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7060 PvuII CAG'CTG Cuts at: 4298 4298 Size: 7060 SalI G'TCGA_C Cuts at: 1936 4402 1936 Size: 2466 4594 SapI GCTCTTCn'nnn_ Cuts at: 2141 2141 Size: 7060 SbfI CC_TGCA'GG Cuts at: 4412 4412 Size: 7060 ScaI AGT'ACT Cuts at: 2094 5308 7020 2094 Size: 3214 1712 2134 Fragments arranged by size: 3214 2134 1712 SfcI C'TryA_G Cuts at: 826 1045 2028 3396 3570 4408 5543 6224 6415 Size: 219 983 1368 174 838 1135 681 191 Cuts at: 6415 826 Size: 1471 Fragments arranged by size: 1471 1368 1135 983 838 681 219 191 174 SfoI GGC'GCC Cuts at: 2010 2010 Size: 7060 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7060 SmaI CCC'GGG Cuts at: 1872 4387 1872 Size: 2515 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 3564 3872 5164 6065 6327 Size: 197 926 177 1444 308 1292 901 262 Cuts at: 6327 820 Size: 1553 Fragments arranged by size: 1553 1444 1292 926 901 308 262 197 177 SnaBI TAC'GTA Cuts at: 493 4851 493 Size: 4358 2702 SpeI A'CTAG_T Cuts at: 152 3143 4174 152 Size: 2991 1031 3038 Fragments arranged by size: 3038 2991 1031 SphI G_CATG'C Cuts at: 1132 2762 4418 1132 Size: 1630 1656 3774 Fragments arranged by size: 3774 1656 1630 SspI AAT'ATT Cuts at: 5 52 4104 4984 5 Size: 47 4052 880 2081 Fragments arranged by size: 4052 2081 880 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7060 StyI C'CwwG_G Cuts at: 513 1065 2004 2128 2324 4063 4499 513 Size: 552 939 124 196 1739 436 3074 Fragments arranged by size: 3074 1739 939 552 436 196 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2593 3387 4292 5087 5104 5257 5442 Size: 1255 1013 794 905 795 17 153 185 Cuts at: 5442 5887 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1013 905 795 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 2594 2644 Size: 274 80 33 51 1443 115 502 50 Cuts at: 2644 3439 3832 3973 5306 7018 96 Size: 795 393 141 1333 1712 138 Fragments arranged by size: 1712 1443 1333 795 502 393 274 141 138 115 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4029 4061 7020 7052 4029 Size: 32 2959 32 4037 Fragments arranged by size: 4037 2959 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7060 XbaI T'CTAG_A Cuts at: 4396 4396 Size: 7060 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7060 XmaI C'CCGG_G Cuts at: 1870 4385 1870 Size: 2515 4545 XmnI GAAnn'nnTTC Cuts at: 2910 3072 5189 2910 Size: 162 2117 4781 Fragments arranged by size: 4781 2117 162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4868 276 Size: 53 83 186 936 3334 2468 Fragments arranged by size: 3334 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI