Restriction map for
pFN21AE0145
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AluI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AvaI AviII AvrII
BalI BamHI BanI BbeI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstF5I BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 133 CGCG CG^CG
AcyI 52 GGCGTC G[GA]^CG[CT]C
AflIII 266 ACGTGT A^C[GA][CT]GT
AhdI 172 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 72 GATCC GGATC(4/5)
AspEI 172 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 49 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 101 GGGCTC G[GA]GC[CT]^C
BbrPI 265 CACGTG CAC^GTG
BbsI 55 GTCTTC GAAGAC(2/6)
BcgI 119 GCAAGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 98 CCGGG CC^[GC]GG
BmyI 101 GGGCTC G[AGT]GC[ACT]^C
BpuAI 55 GTCTTC GAAGAC(2/6)
BsaAI 265 CACGTG [CT]AC^GT[GA]
BsaBI 72 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 52 GGCGTC G[GA]^CG[CT]C
Bsp1286I 101 GGGCTC G[AGT]GC[ACT]^C
BsrBI 93 CCGCTC CCGCTC(-3/-3)
BstEII 351 GGTCACC G^GT[ACGT]ACC
BstNI 356 CCTGG CC^[AT]GG
BstPI 351 GGTCACC G^GT[ACGT]ACC
BstUI 133 CGCG CG^CG
DrdI 343 GACTACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 281 CCATGG C^C[GA][CT]GG
Eam1105I 172 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 351 GGTCACC G^GT[ACGT]ACC
EcoRII 356 CCTGG ^CC[AT]GG
EcoT14I 281 CCATGG C^C[AT][AT]GG
HaeIII 285 GGCC GG^CC
HgaI 53 GCGTC GACGC(5/10)
Hin1I 52 GGCGTC G[GA]^CG[CT]C
HphI 353 TCACC GGTGA(8/7)
MaeII 266 ACGT A^CGT
MaeIII 352 GTCAC ^GT[ACGT]AC
MamI 72 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 356 CCTGG CC^[AT]GG
MvnI 133 CGCG CG^CG
NciI 98 CCGGG CC^[GC]GG
NcoI 281 CCATGG C^CATGG
NspI 188 ACATGC [GA]CATG^[CT]
PflFI 49 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 265 CACGTG CAC^GTG
PmlI 265 CACGTG CAC^GTG
PstI 110 CTGCAG CTGCA^G
SfcI 110 CTGCAG C^T[GA][CT]AG
StyI 281 CCATGG C^C[AT][AT]GG
Tsp45I 352 GTCAC ^GT[GC]AC
Tth111I 49 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AvaII 12 GGACC G^G[AT]CC
292 GGTCC
BbvI 109 GCTGC GCAGC(8/12)
203 GCTGC
BssKI 98 CCGGG ^CC[ACGT]GG
356 CCTGG
Cac8I 227 GCGGGC GC[ACGT]^[ACGT]GC
303 GCACGC
CfoI 132 GCGC GCG^C
231 GCGC
Cfr13I 12 GGACC G^G[ACGT]CC
292 GGTCC
DdeI 149 CTGAG C^T[ACGT]AG
317 CTGAG
DpnII 72 GATC ^GATC
126 GATC
FauI 92 CCCGC CCCGC(4/6)
227 GCGGG
HapII 98 CCGG C^CGG
106 CCGG
HhaI 132 GCGC GCG^C
231 GCGC
HinP1I 132 GCGC G^CGC
231 GCGC
HpaII 98 CCGG C^CGG
106 CCGG
MboI 72 GATC ^GATC
126 GATC
MboII 258 GAAGA GAAGA(8/7)
56 TCTTC
MspI 98 CCGG C^CGG
106 CCGG
NdeII 72 GATC ^GATC
126 GATC
NlaIV 87 GGCTCC GG[ACGT]^[ACGT]CC
102 GGCTCC
PleI 43 GACTC GAGTC(4/5)
246 GAGTC
Sau3AI 72 GATC ^GATC
126 GATC
Sau96I 12 GGACC G^G[ACGT]CC
292 GGTCC
ScrFI 98 CCGGG CC^[ACGT]GG
356 CCTGG
TfiI 77 GAATC G^A[AT]TC
261 GATTC
TseI 109 GCTGC G^C[AT]GC
203 GCTGC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI AfaI BsaJI BsiYI BslI
Fnu4HI HinfI ItaI MnlI MwoI
NlaIII RsaI