Restriction map for
pFN21AB6309
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaI
BseAI BsgI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AfaI 273 GTAC GT^AC
AlwNI 46 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 267 GAGCAC G[AT]GC[AT]^C
BbvCI 367 GCTGAGG CCTCAGC(-5/-2)
BclI 329 TGATCA T^GATCA
BlpI 206 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 206 GCTTAGC GC^T[ACGT]AGC
BsaAI 270 CACGTA [CT]AC^GT[GA]
BsaWI 284 ACCGGA [AT]^CCGG[AT]
BseRI 358 GAGGAG GAGGAG(10/8)
BsiEI 728 CGGCCG CG[GA][CT]^CG
BsiHKAI 267 GAGCAC G[AT]GC[AT]^C
BsiWI 272 CGTACG C^GTACG
BsmBI 150 CGTCTC CGTCTC(1/5)
BsmFI 407 GGGAC GGGAC(10/14)
BsrBI 499 GAGCGG CCGCTC(-3/-3)
BsrFI 9 GCCGGC [GA]^CCGG[CT]
BsrI 610 ACTGG ACTGG(1/-1)
BssSI 244 CTCGTG CACGAG(-5/-1)
BstF5I 414 CATCC GGATG(2/0)
CelII 206 GCTTAGC GC^T[ACGT]AGC
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
DraIII 548 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 728 CGGCCG C^GGCCG
EarI 472 CTCTTC CTCTTC(1/4)
EclXI 728 CGGCCG C^GGCCG
Eco52I 728 CGGCCG C^GGCCG
EcoNI 489 CCTCCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 402 CCAAGG C^C[AT][AT]GG
FbaI 329 TGATCA T^GATCA
FokI 414 CATCC GGATG(9/13)
Ksp632I 472 CTCTTC CTCTTC(1/4)
MaeII 271 ACGT A^CGT
MseI 338 TTAA T^TAA
NaeI 9 GCCGGC GCC^GGC
NgoMIV 9 GCCGGC G^CCGGC
NlaIII 92 CATG CATG^
NotI 727 GCGGCCGC GC^GGCCGC
NspI 91 GCATGC [GA]CATG^[CT]
PaeR7I 36 CTCGAG C^TCGAG
PflMI 45 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 273 GTAC GT^AC
SfcI 493 CTACAG C^T[GA][CT]AG
SfiI 3 GGCCGCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 15 CCCGGG CCC^GGG
SmlI 36 CTCGAG C^T[CT][GA]AG
SphI 91 GCATGC GCATG^C
StyI 402 CCAAGG C^C[AT][AT]GG
Tru9I 338 TTAA T^TAA
TspRI 525 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 45 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 36 CTCGAG C^TCGAG
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 312 GATCC GGATC(4/5)
435 GATCC
ApaI 107 GGGCCC GGGCC^C
447 GGGCCC
AvaII 651 GGACC G^G[AT]CC
684 GGTCC
BanII 107 GGGCCC G[GA]GC[CT]^C
447 GGGCCC
BglI 4 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
131 GCCTGTCGGGC
BpmI 300 CTCCAG CTGGAG(16/14)
611 CTGGAG
BsmAI 390 GAGAC GTCTC(1/5)
151 GTCTC
BspMI 428 ACCTGC ACCTGC(4/8)
463 ACCTGC
BssHII 84 GCGCGC G^CGCGC
211 GCGCGC
DraII 106 GGGGCCC [GA]G^G[ACGT]CC[CT]
447 GGGCCCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
728 CGGCCG
EcoO109I 106 GGGGCCC [GA]G^G[ACGT]CC[CT]
447 GGGCCCC
HaeII 60 GGCGCT [GA]GCGC^[CT]
239 AGCGCC
HgaI 219 GACGC GACGC(5/10)
288 GACGC
HinfI 409 GACTC G^A[ACGT]TC
529 GACTC
HphI 327 GGTGA GGTGA(8/7)
332 TCACC
MslI 24 CATCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
597 CATCTTTGTG
PleI 409 GACTC GAGTC(4/5)
529 GACTC
PvuII 70 CAGCTG CAG^CTG
626 CAGCTG
SfaNI 156 GATGC GCATC(5/9)
291 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaI BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmI BsoBI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NheI NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
Tsp45I TthHB8I