(Circular) (Six-base) MAPSORT of: pFN21AE0555.seq Check: 7108 from: 1 to: 5836 pFN21AE0555 5836 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2808 3179 1937 Size: 871 371 4594 Fragments arranged by size: 4594 871 371 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 2520 2864 3899 5283 5551 1632 Size: 818 70 344 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 818 344 268 70 AfeI AGC'GCT Cuts at: 2096 2120 2096 Size: 24 5812 AflII C'TTAA_G Cuts at: 820 1017 2419 820 Size: 197 1402 4237 Fragments arranged by size: 4237 1402 197 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2332 2364 2332 Size: 32 5804 AlwNI CAG_nnn'CTG Cuts at: 2498 3579 5151 2498 Size: 1081 1572 3183 Fragments arranged by size: 3183 1572 1081 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2856 3012 3145 3371 3523 5632 1493 Size: 1363 156 133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1363 226 156 152 133 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2132 3161 1870 Size: 73 189 1029 4545 Fragments arranged by size: 4545 1029 189 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 4612 618 Size: 325 217 627 1370 1455 1842 Fragments arranged by size: 1842 1455 1370 627 325 217 BanII G_rGCy'C Cuts at: 1391 2464 3155 1391 Size: 1073 691 4072 Fragments arranged by size: 4072 1073 691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2265 2797 928 Size: 971 366 532 3967 Fragments arranged by size: 3967 971 532 366 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BfrBI ATG'CAT Cuts at: 2969 2969 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4447 136 Size: 107 122 71 631 605 337 2438 1525 Fragments arranged by size: 2438 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3070 5831 3070 Size: 2761 3075 BlpI GC'TnA_GC Cuts at: 2446 3253 2446 Size: 807 5029 Bme1580I G_kGCm'C Cuts at: 1790 3897 5053 1790 Size: 2107 1156 2573 Fragments arranged by size: 2573 2107 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2033 1056 Size: 977 4859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 4496 1239 Size: 848 2409 2579 Fragments arranged by size: 2579 2409 848 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2476 2892 3961 4826 5124 2476 Size: 416 1069 865 298 3188 Fragments arranged by size: 3188 1069 865 416 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2080 3627 493 Size: 711 876 1547 2702 Fragments arranged by size: 2702 1547 876 711 BsaBI GATnn'nnATC Cuts at: 3075 5830 3075 Size: 2755 3081 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2473 2503 1178 Size: 30 165 30 1070 30 4511 Fragments arranged by size: 4511 1070 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5836 BseYI C'CCAG_C Cuts at: 5039 5535 5039 Size: 496 5340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2511 1163 Size: 573 775 4488 Fragments arranged by size: 4488 775 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3897 3982 5053 3897 Size: 85 1071 4680 Fragments arranged by size: 4680 1071 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 BsmBI CGTCTCn'nnnn_ Cuts at: 2406 2406 Size: 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2464 3155 3897 3982 5053 1391 Size: 399 674 691 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 691 674 399 85 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2233 3191 844 Size: 1289 100 958 3489 Fragments arranged by size: 3489 1289 958 100 BsrBI CCG'CTC Cuts at: 1394 1823 2040 2053 3723 1394 Size: 429 217 13 1670 3507 Fragments arranged by size: 3507 1670 429 217 13 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 2379 2682 4331 4505 65 Size: 1183 1119 12 303 1649 174 1396 Fragments arranged by size: 1649 1396 1183 1119 303 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 3121 96 Size: 3025 2811 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2162 794 Size: 1368 4468 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2979 3070 3166 3533 3918 3935 5387 5473 Size: 1007 91 96 367 385 17 1452 86 Cuts at: 5473 5485 5831 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1007 385 367 346 96 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2512 3352 4146 4166 2512 Size: 840 794 20 4182 Fragments arranged by size: 4182 840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2362 2939 Size: 54 1425 50 275 18 180 352 577 Cuts at: 2939 3283 4172 5801 8 Size: 344 889 1629 43 Fragments arranged by size: 1629 1425 889 577 352 344 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2302 3766 1353 Size: 949 1464 3423 Fragments arranged by size: 3423 1464 949 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2450 2520 2864 3899 4496 5283 Size: 393 455 363 70 344 1035 597 787 Cuts at: 5283 5551 1239 Size: 268 1524 Fragments arranged by size: 1524 1035 787 597 455 393 363 344 268 70 EcoO109I rG'GnC_Cy Cuts at: 2245 2245 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2933 2933 Size: 5836 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2098 2122 4983 1678 Size: 420 24 2861 2531 Fragments arranged by size: 2861 2531 420 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373 Size: 32 135 32 2905 32 42 32 2626 Fragments arranged by size: 2905 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2148 3180 3426 669 Size: 988 281 210 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 210 HindIII A'AGCT_T Cuts at: 748 2439 2846 748 Size: 1691 407 3738 Fragments arranged by size: 3738 1691 407 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 2151 3435 2151 Size: 1284 4552 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 4950 5134 1305 Size: 861 2784 184 2007 Fragments arranged by size: 2784 2007 861 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2364 2941 10 Size: 54 1425 343 532 577 2905 Fragments arranged by size: 2905 1425 577 532 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2352 2372 2579 2955 3794 4153 4312 Size: 648 1186 20 207 376 839 359 159 Cuts at: 4312 518 Size: 2042 Fragments arranged by size: 2042 1186 839 648 376 359 207 159 20 MspA1I CmG'CkG Cuts at: 1606 2470 3252 3929 5077 5322 1606 Size: 864 782 677 1148 245 2120 Fragments arranged by size: 2120 1148 864 782 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 2029 1052 Size: 977 4859 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NsiI A_TGCA'T Cuts at: 2971 2971 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 2521 2969 3194 4739 1132 Size: 97 1292 448 225 1545 2229 Fragments arranged by size: 2229 1545 1292 448 225 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PfoI T'CCnGG_A Cuts at: 2335 2335 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PmlI CAC'GTG Cuts at: 2080 2080 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2245 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 2244 3188 830 Size: 1414 944 3478 Fragments arranged by size: 3478 1414 944 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2470 2470 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2302 2302 Size: 5836 SbfI CC_TGCA'GG Cuts at: 2244 3188 2244 Size: 944 4892 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2240 2805 3184 4319 5000 5191 826 Size: 219 1195 565 379 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 681 565 379 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2419 2455 2907 3940 4841 Size: 197 926 189 287 36 452 1033 901 Cuts at: 4841 5103 820 Size: 262 1553 Fragments arranged by size: 1553 1033 926 901 452 287 262 197 189 36 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 2969 3194 1132 Size: 1837 225 3774 Fragments arranged by size: 3774 1837 225 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 3093 3275 513 Size: 552 939 1089 182 3074 Fragments arranged by size: 3074 1089 939 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2818 3863 3880 4033 4218 4663 325 Size: 1255 1238 1045 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1238 1045 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 3121 4082 Size: 274 80 33 51 1443 552 592 961 Cuts at: 4082 5794 96 Size: 1712 138 Fragments arranged by size: 1712 1443 961 592 552 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2473 2505 5796 5828 2473 Size: 32 3291 32 2481 Fragments arranged by size: 3291 2481 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3067 3172 3067 Size: 105 5731 XhoI C'TCGA_G Cuts at: 1943 2132 1943 Size: 189 5647 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3063 3965 3063 Size: 902 4934 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI