(Circular) (Six-base) MAPSORT of: pFN21AA0347.seq Check: 4881 from: 1 to: 8470 pFN21AA0347 8470 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2434 3746 2434 Size: 1312 7158 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6280 278 Size: 53 83 186 936 4744 2468 Fragments arranged by size: 4744 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5813 1937 Size: 3876 4594 Acc65I G'GTAC_C Cuts at: 5791 5791 Size: 8470 AclI AA'CG_TT Cuts at: 1563 6597 6970 1563 Size: 5034 373 3063 Fragments arranged by size: 5034 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2653 3833 5531 6533 7917 8185 1632 Size: 746 275 1180 1698 1002 1384 268 1917 Fragments arranged by size: 1917 1698 1384 1180 1002 746 275 268 AfeI AGC'GCT Cuts at: 3849 3849 Size: 8470 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8273 AflIII A'CryG_T Cuts at: 1203 3330 4509 7369 8346 1203 Size: 2127 1179 2860 977 1327 Fragments arranged by size: 2860 2127 1327 1179 977 AgeI A'CCGG_T Cuts at: 7301 7301 Size: 8470 AhdI GACnn_n'nnGTC Cuts at: 7199 7199 Size: 8470 AleI CACnn'nnGTG Cuts at: 1166 2499 3120 1166 Size: 1333 621 6516 Fragments arranged by size: 6516 1333 621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4686 1184 Size: 3502 4968 AlwNI CAG_nnn'CTG Cuts at: 2175 2476 3085 4595 4640 5054 5563 6213 7785 Size: 301 609 1510 45 414 509 650 1572 Cuts at: 7785 2175 Size: 2860 Fragments arranged by size: 2860 1572 1510 650 609 509 414 301 45 ApaI G_GGCC'C Cuts at: 3317 4124 4452 3317 Size: 807 328 7335 Fragments arranged by size: 7335 807 328 ApaLI G'TGCA_C Cuts at: 2954 6527 7683 2954 Size: 3573 1156 3741 Fragments arranged by size: 3741 3573 1156 ApoI r'AATT_y Cuts at: 1493 2021 2979 2997 3549 5473 5779 6005 6157 Size: 528 958 18 552 1924 306 226 152 Cuts at: 6157 8266 1493 Size: 2109 1697 Fragments arranged by size: 2109 1924 1697 958 552 528 306 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 7024 160 Size: 872 5992 1606 Fragments arranged by size: 5992 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8470 AvaI C'yCGr_G Cuts at: 1870 1943 3707 4416 4747 4899 5085 5209 5266 Size: 73 1764 709 331 152 186 124 57 Cuts at: 5266 5795 1870 Size: 529 4545 Fragments arranged by size: 4545 1764 709 529 331 186 152 124 73 57 BamHI G'GATC_C Cuts at: 5404 5735 5800 6167 5404 Size: 331 65 367 7707 Fragments arranged by size: 7707 367 331 65 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2698 4612 4705 5104 5791 Size: 325 217 627 911 1914 93 399 687 Cuts at: 5791 7246 618 Size: 1455 1842 Fragments arranged by size: 1914 1842 1455 911 687 627 399 325 217 93 BanII G_rGCy'C Cuts at: 1391 2058 2067 3317 3390 4124 4161 4452 5216 Size: 667 9 1250 73 734 37 291 764 Cuts at: 5216 5240 5789 1391 Size: 24 549 4072 Fragments arranged by size: 4072 1250 764 734 667 549 291 73 37 24 9 BbeI G_GCGC'C Cuts at: 2702 4709 5108 2702 Size: 2007 399 6064 Fragments arranged by size: 6064 2007 399 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 5730 928 Size: 971 402 3429 3668 Fragments arranged by size: 3668 3429 971 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2564 2598 4012 4046 6661 6695 1903 Size: 34 627 34 1414 34 2615 34 3678 Fragments arranged by size: 3678 2615 1414 627 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2006 6362 7572 2006 Size: 4356 1210 2904 Fragments arranged by size: 4356 2904 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8470 BfrBI ATG'CAT Cuts at: 2796 2796 Size: 8470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4119 4703 4901 Size: 107 122 71 631 605 2447 584 198 Cuts at: 4901 7081 136 Size: 2180 1525 Fragments arranged by size: 2447 2180 1525 631 605 584 198 122 107 71 BglII A'GATC_T Cuts at: 8465 8465 Size: 8470 BlpI GC'TnA_GC Cuts at: 5203 5887 5203 Size: 684 7786 Bme1580I G_kGCm'C Cuts at: 1790 2517 2958 3317 4124 4452 4539 4650 6531 Size: 727 441 359 807 328 87 111 1881 Cuts at: 6531 7687 1790 Size: 1156 2573 Fragments arranged by size: 2573 1881 1156 807 727 441 359 328 111 87 BmgBI CAC'GTC Cuts at: 1318 2512 3331 4512 5160 5617 1318 Size: 1194 819 1181 648 457 4171 Fragments arranged by size: 4171 1194 1181 819 648 457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2031 3092 4510 Size: 416 53 83 399 24 614 1061 1418 Cuts at: 4510 4544 4725 5258 6201 7159 442 Size: 34 181 533 943 958 1753 Fragments arranged by size: 1753 1418 1061 958 943 614 533 416 399 181 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8470 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3934 4846 3934 Size: 912 7558 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 3424 3487 3624 3904 3921 4643 5012 Size: 862 1323 63 137 280 17 722 369 Cuts at: 5012 5037 5513 7130 1239 Size: 25 476 1617 2579 Fragments arranged by size: 2579 1617 1323 862 722 476 369 280 137 63 25 17 Bpu10I CC'TnA_GC Cuts at: 3851 3851 Size: 8470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2772 6595 7460 7758 2772 Size: 3823 865 298 3484 Fragments arranged by size: 3823 3484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 4145 7133 882 Size: 617 624 2022 2988 2219 Fragments arranged by size: 2988 2219 2022 624 617 BsaAI yAC'GTr Cuts at: 493 1204 6261 493 Size: 711 5057 2702 Fragments arranged by size: 5057 2702 711 BsaBI GATnn'nnATC Cuts at: 8464 8464 Size: 8470 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2699 2879 3995 4706 Size: 53 83 186 936 1166 180 1116 711 Cuts at: 4706 5105 5148 5259 6277 6659 7294 275 Size: 399 43 111 1018 382 635 1451 Fragments arranged by size: 1451 1166 1116 1018 936 711 635 399 382 186 180 111 83 53 43 BsaWI w'CCGG_w Cuts at: 2218 2832 4349 4436 6903 7301 7575 7722 8133 Size: 614 1517 87 2467 398 274 147 411 Cuts at: 8133 2218 Size: 2555 Fragments arranged by size: 2555 2467 1517 614 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2496 2501 2526 2531 3422 Size: 30 165 30 1093 5 25 5 891 Cuts at: 3422 3452 5511 5541 1178 Size: 30 2059 30 4107 Fragments arranged by size: 4107 2059 1093 891 165 30 30 30 30 25 5 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2546 2816 3371 3490 3553 3725 4229 5317 Size: 1360 270 555 119 63 172 504 1088 Cuts at: 5317 1186 Size: 4339 Fragments arranged by size: 4339 1360 1088 555 504 270 172 119 63 BseYI C'CCAG_C Cuts at: 2030 2067 2072 2197 3374 3649 3832 3844 4171 Size: 37 5 125 1177 275 183 12 327 Cuts at: 4171 4528 4781 4853 7673 8169 2030 Size: 357 253 72 2820 496 2331 Fragments arranged by size: 2820 2331 1177 496 357 327 275 253 183 125 72 37 12 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2785 3128 3334 3353 4033 4182 1163 Size: 573 1049 343 206 19 680 149 5451 Fragments arranged by size: 5451 1049 680 573 343 206 149 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5920 6681 7709 1540 Size: 275 186 3919 761 1028 2301 Fragments arranged by size: 3919 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 2958 3105 3390 3483 4436 6531 6616 7687 Size: 399 147 285 93 953 2095 85 1071 Cuts at: 7687 2559 Size: 3342 Fragments arranged by size: 3342 2095 1071 953 399 285 147 93 85 BsmI GAATG_Cn' Cuts at: 2575 2798 3378 4718 5981 6074 2575 Size: 223 580 1340 1263 93 4971 Fragments arranged by size: 4971 1340 1263 580 223 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2067 2517 2559 2958 3105 3317 Size: 399 268 9 450 42 399 147 212 Cuts at: 3317 3390 3483 4124 4161 4436 4452 4539 4650 Size: 73 93 641 37 275 16 87 111 Cuts at: 4650 5216 5240 5789 6531 6616 7687 1391 Size: 566 24 549 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 641 566 549 450 399 399 275 268 212 147 111 93 87 85 73 42 37 24 16 9 BspEI T'CCGG_A Cuts at: 2832 4349 8133 2832 Size: 1517 3784 3169 Fragments arranged by size: 3784 3169 1517 BspHI T'CATG_A Cuts at: 3595 5445 6359 8089 3595 Size: 1850 914 1730 3976 Fragments arranged by size: 3976 1850 1730 914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2434 3746 4770 4989 5825 844 Size: 1590 1312 1024 219 836 3489 Fragments arranged by size: 3489 1590 1312 1024 836 219 BsrBI CCG'CTC Cuts at: 1394 1823 4942 6357 1394 Size: 429 3119 1415 3507 Fragments arranged by size: 3507 3119 1415 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3802 6965 7139 65 Size: 1183 2554 3163 174 1396 Fragments arranged by size: 3163 2554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3405 7114 7301 1700 Size: 110 1595 3709 187 2869 Fragments arranged by size: 3709 2869 1595 187 110 BsrGI T'GTAC_A Cuts at: 96 2619 96 Size: 2523 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8470 BssSI C'ACGA_G Cuts at: 2843 3104 3476 6530 7542 2843 Size: 261 372 3054 1012 3771 Fragments arranged by size: 3771 3054 1012 372 261 BstAPI GCAn_nnn'nTGC Cuts at: 794 5563 794 Size: 4769 3701 BstBI TT'CG_AA Cuts at: 2312 7274 2312 Size: 4962 3508 BstEII G'GTnAC_C Cuts at: 1184 2492 4497 1184 Size: 1308 2005 5157 Fragments arranged by size: 5157 2005 1308 BstXI CCAn_nnnn'nTGG Cuts at: 2407 2686 5617 2407 Size: 279 2931 5260 Fragments arranged by size: 5260 2931 279 BstYI r'GATC_y Cuts at: 1972 2613 3156 3399 4298 4439 5404 5735 5800 Size: 641 543 243 899 141 965 331 65 Cuts at: 5800 6167 6552 6569 8021 8107 8119 8465 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 965 899 641 543 385 367 346 331 243 141 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2429 3078 5004 2429 Size: 649 1926 5895 Fragments arranged by size: 5895 1926 649 BtgI C'CryG_G Cuts at: 513 1065 2058 2148 2526 3263 3675 4650 4920 Size: 552 993 90 378 737 412 975 270 Cuts at: 4920 5022 513 Size: 102 3961 Fragments arranged by size: 3961 993 975 737 552 412 378 270 102 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2688 487 Size: 683 56 1462 6269 Fragments arranged by size: 6269 1462 683 56 BtsI GCAGTG_nn' Cuts at: 2084 2116 2176 2773 3968 4273 4568 5986 6780 Size: 32 60 597 1195 305 295 1418 794 Cuts at: 6780 6800 2084 Size: 20 3754 Fragments arranged by size: 3754 1418 1195 794 597 305 295 60 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8435 DraI TTT'AAA Cuts at: 5774 5774 Size: 8470 DraIII CAC_nnn'GTG Cuts at: 2563 2710 2563 Size: 147 8323 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5441 7477 809 Size: 507 4125 2036 1802 Fragments arranged by size: 4125 2036 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 2589 3141 Size: 54 1425 50 275 18 579 180 552 Cuts at: 3141 3517 4917 5917 6806 8435 8 Size: 376 1400 1000 889 1629 43 Fragments arranged by size: 1629 1425 1400 1000 889 579 552 376 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5917 1537 Size: 275 4105 4090 Fragments arranged by size: 4105 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 3809 5754 6400 1353 Size: 1101 1355 1945 646 3423 Fragments arranged by size: 3423 1945 1355 1101 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2016 4447 5078 7057 7431 7577 Size: 1064 138 427 2431 631 1979 374 146 Cuts at: 7577 387 Size: 1280 Fragments arranged by size: 2431 1979 1280 1064 631 427 374 146 138 EcoICRI GAG'CTC Cuts at: 3388 3388 Size: 8470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2378 2653 3424 3487 3624 3833 Size: 393 469 277 275 771 63 137 209 Cuts at: 3833 3904 3921 4643 5012 5037 5513 5531 6533 Size: 71 17 722 369 25 476 18 1002 Cuts at: 6533 7130 7917 8185 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1002 787 771 722 597 476 469 393 369 277 275 268 209 137 71 63 25 18 17 EcoNI CCTnn'n_nnAGG Cuts at: 2891 3077 4181 4766 2891 Size: 186 1104 585 6595 Fragments arranged by size: 6595 1104 585 186 EcoO109I rG'GnC_Cy Cuts at: 2080 3082 3129 3314 3364 3471 4121 4448 4752 Size: 1002 47 185 50 107 650 327 304 Cuts at: 4752 4929 5194 2080 Size: 177 265 5356 Fragments arranged by size: 5356 1002 650 327 304 265 185 177 107 50 47 EcoRI G'AATT_C Cuts at: 2979 5779 6157 2979 Size: 2800 378 5292 Fragments arranged by size: 5292 2800 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2767 4177 4362 2767 Size: 1410 185 6875 Fragments arranged by size: 6875 1410 185 FspI TGC'GCA Cuts at: 6976 6976 Size: 8470 HaeII r_GCGC'y Cuts at: 1678 2702 3851 4589 4709 5108 7617 1678 Size: 1024 1149 738 120 399 2509 2531 Fragments arranged by size: 2531 2509 1149 1024 738 399 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 2792 2824 3210 Size: 32 135 32 929 32 259 32 386 Cuts at: 3210 3242 5111 5143 5186 5218 7111 7143 7185 Size: 32 1869 32 43 32 1893 32 42 Cuts at: 7185 7217 1373 Size: 32 2626 Fragments arranged by size: 2626 1893 1869 929 386 259 135 43 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5814 6060 669 Size: 988 281 3876 246 3079 Fragments arranged by size: 3876 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2744 748 Size: 1996 6474 HpaI GTT'AAC Cuts at: 6060 6060 Size: 8470 KasI G'GCGC_C Cuts at: 2698 4705 5104 2698 Size: 2007 399 6064 Fragments arranged by size: 6064 2007 399 KpnI G_GTAC'C Cuts at: 5795 5795 Size: 8470 MfeI C'AATT_G Cuts at: 6069 6069 Size: 8470 MluI A'CGCG_T Cuts at: 8346 8346 Size: 8470 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3213 5691 7584 7768 1305 Size: 1908 2478 1893 184 2007 Fragments arranged by size: 2478 2007 1908 1893 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 3143 10 Size: 54 1425 343 579 732 5337 Fragments arranged by size: 5337 1425 732 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2499 2847 3120 5363 5615 6428 6787 Size: 648 1333 348 273 2243 252 813 359 Cuts at: 6787 6946 518 Size: 159 2042 Fragments arranged by size: 2243 2042 1333 813 648 359 348 273 252 159 MspA1I CmG'CkG Cuts at: 1606 2473 3243 3435 3653 3759 4471 4524 4990 Size: 867 770 192 218 106 712 53 466 Cuts at: 4990 5054 5461 5886 6563 7711 7956 1606 Size: 64 407 425 677 1148 245 2120 Fragments arranged by size: 2120 1148 867 770 712 677 466 425 407 245 218 192 106 64 53 NarI GG'CG_CC Cuts at: 2699 4706 5105 2699 Size: 2007 399 6064 Fragments arranged by size: 6064 2007 399 NcoI C'CATG_G Cuts at: 513 1065 3263 3675 4920 5022 513 Size: 552 2198 412 1245 102 3961 Fragments arranged by size: 3961 2198 1245 552 412 102 NdeI CA'TA_TG Cuts at: 387 5671 387 Size: 5284 3186 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8470 NotI GC'GGCC_GC Cuts at: 5917 5917 Size: 8470 NsiI A_TGCA'T Cuts at: 2798 2798 Size: 8470 NspI r_CATG'y Cuts at: 1132 1229 2764 5828 7373 1132 Size: 97 1535 3064 1545 2229 Fragments arranged by size: 3064 2229 1545 1535 97 PasI CC'CwG_GG Cuts at: 1348 3887 1348 Size: 2539 5931 PciI A'CATG_T Cuts at: 7369 7369 Size: 8470 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 3899 4494 1108 Size: 1932 859 595 5084 Fragments arranged by size: 5084 1932 859 595 PfoI T'CCnGG_A Cuts at: 3033 3033 Size: 8470 PmeI GTTT'AAAC Cuts at: 5774 5774 Size: 8470 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6539 6571 8052 8084 6539 Size: 32 1481 32 6925 Fragments arranged by size: 6925 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 3082 3471 4752 5194 2080 Size: 1002 389 1281 442 5356 Fragments arranged by size: 5356 1281 1002 442 389 PshAI GACnn'nnGTC Cuts at: 1613 5292 1613 Size: 3679 4791 PsiI TTA'TAA Cuts at: 6040 6040 Size: 8470 PspOMI G'GGCC_C Cuts at: 3313 4120 4448 3313 Size: 807 328 7335 Fragments arranged by size: 7335 807 328 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3206 3238 3206 Size: 32 8438 PstI C_TGCA'G Cuts at: 830 2091 2297 2377 3165 3420 4233 4974 4989 Size: 1261 206 80 788 255 813 741 15 Cuts at: 4989 5403 5822 830 Size: 414 419 3478 Fragments arranged by size: 3478 1261 813 788 741 419 414 255 206 80 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8470 PvuII CAG'CTG Cuts at: 2473 3243 3653 3759 4524 4990 5054 5461 2473 Size: 770 410 106 765 466 64 407 5482 Fragments arranged by size: 5482 770 765 466 410 407 106 64 SacI G_AGCT'C Cuts at: 3390 3390 Size: 8470 SalI G'TCGA_C Cuts at: 1936 5812 1936 Size: 3876 4594 SanDI GG'GwC_CC Cuts at: 3471 4752 3471 Size: 1281 7189 SapI GCTCTTCn'nnn_ Cuts at: 5754 5754 Size: 8470 SbfI CC_TGCA'GG Cuts at: 5403 5822 5403 Size: 419 8051 ScaI AGT'ACT Cuts at: 6718 8430 6718 Size: 1712 6758 SexAI A'CCwGG_T Cuts at: 2631 2886 2631 Size: 255 8215 SfcI C'TryA_G Cuts at: 826 1045 2087 2293 2373 3161 3416 4018 4229 Size: 219 1042 206 80 788 255 602 211 Cuts at: 4229 4970 4985 5055 5399 5519 5818 6953 7634 Size: 741 15 70 344 120 299 1135 681 Cuts at: 7634 7825 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1042 788 741 681 602 344 299 255 219 211 206 191 120 80 70 15 SfoI GGC'GCC Cuts at: 2700 4707 5106 2700 Size: 2007 399 6064 Fragments arranged by size: 6064 2007 399 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8470 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8470 SmaI CCC'GGG Cuts at: 1872 4901 5797 1872 Size: 3029 896 4545 Fragments arranged by size: 4545 3029 896 SmlI C'TyrA_G Cuts at: 820 1017 1943 2787 4416 6574 7475 7737 820 Size: 197 926 844 1629 2158 901 262 1553 Fragments arranged by size: 2158 1629 1553 926 901 844 262 197 SnaBI TAC'GTA Cuts at: 493 6261 493 Size: 5768 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8470 SphI G_CATG'C Cuts at: 1132 5828 1132 Size: 4696 3774 SspI AAT'ATT Cuts at: 5 52 5309 5659 6394 5 Size: 47 5257 350 735 2081 Fragments arranged by size: 5257 2081 735 350 47 StuI AGG'CCT Cuts at: 1517 4772 1517 Size: 3255 5215 StyI C'CwwG_G Cuts at: 513 1065 2049 2388 3263 3675 4182 4302 4364 Size: 552 984 339 875 412 507 120 62 Cuts at: 4364 4920 5022 5909 513 Size: 556 102 887 3074 Fragments arranged by size: 3074 984 887 875 556 552 507 412 339 120 102 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3026 6497 6514 6667 6852 7297 325 Size: 1255 1446 3471 17 153 185 445 1498 Fragments arranged by size: 3471 1498 1446 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 3222 6716 Size: 274 80 33 51 1443 642 603 3494 Cuts at: 6716 8428 96 Size: 1712 138 Fragments arranged by size: 3494 1712 1443 642 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 3422 3454 8430 8462 2496 Size: 32 894 32 4976 32 2504 Fragments arranged by size: 4976 2504 894 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3770 1316 Size: 2454 6016 XbaI T'CTAG_A Cuts at: 5806 5806 Size: 8470 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2056 4036 2056 Size: 1980 6490 XhoI C'TCGA_G Cuts at: 1943 4416 1943 Size: 2473 5997 XmaI C'CCGG_G Cuts at: 1870 4899 5795 1870 Size: 3029 896 4545 Fragments arranged by size: 4545 3029 896 XmnI GAAnn'nnTTC Cuts at: 2810 4345 4357 5682 6599 2810 Size: 1535 12 1325 917 4681 Fragments arranged by size: 4681 1535 1325 917 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6278 276 Size: 53 83 186 936 4744 2468 Fragments arranged by size: 4744 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI RsrII SacII SfiI SrfI SwaI