Restriction map for
pFN21AA0347
(Linear) (Six-base) MAPSORT of: KIAA0347.seq Check: 7436 from: 1 to: 3765
KIAA0347.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 429 1741 3765
Size: 429 1312 2024
Fragments arranged by size:
2024 1312 429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 648 1828 3526 3765
Size: 373 275 1180 1698 239
Fragments arranged by size:
1698 1180 373 275 239
AfeI AGC'GCT
Cuts at: 0 1844 3765
Size: 1844 1921
AflIII A'CryG_T
Cuts at: 0 1325 2504 3765
Size: 1325 1179 1261
Fragments arranged by size:
1325 1261 1179
AleI CACnn'nnGTG
Cuts at: 0 494 1115 3765
Size: 494 621 2650
Fragments arranged by size:
2650 621 494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2681 3765
Size: 2681 1084
AlwNI CAG_nnn'CTG
Cuts at: 0 170 471 1080 2590 2635 3049 3558 3765
Size: 170 301 609 1510 45 414 509 207
Fragments arranged by size:
1510 609 509 414 301 207 170 45
ApaI G_GGCC'C
Cuts at: 0 1312 2119 2447 3765
Size: 1312 807 328 1318
Fragments arranged by size:
1318 1312 807 328
ApaLI G'TGCA_C
Cuts at: 0 949 3765
Size: 949 2816
ApoI r'AATT_y
Cuts at: 0 16 974 992 1544 3468 3765
Size: 16 958 18 552 1924 297
Fragments arranged by size:
1924 958 552 297 18 16
AvaI C'yCGr_G
Cuts at: 0 1702 2411 2742 2894 3080 3204 3261 3765
Size: 1702 709 331 152 186 124 57 504
Fragments arranged by size:
1702 709 504 331 186 152 124 57
BamHI G'GATC_C
Cuts at: 0 3399 3730 3765
Size: 3399 331 35
Fragments arranged by size:
3399 331 35
BanI G'GyrC_C
Cuts at: 0 693 2607 2700 3099 3765
Size: 693 1914 93 399 666
Fragments arranged by size:
1914 693 666 399 93
BanII G_rGCy'C
Cuts at: 0 53 62 1312 1385 2119 2156 2447 3211
Size: 53 9 1250 73 734 37 291 764
Cuts at: 3211 3235 3765
Size: 24 530
Fragments arranged by size:
1250 764 734 530 291 73 53 37
24 9
BbeI G_GCGC'C
Cuts at: 0 697 2704 3103 3765
Size: 697 2007 399 662
Fragments arranged by size:
2007 697 662 399
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 3725 3765
Size: 296 3429 40
Fragments arranged by size:
3429 296 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 593 2007 2041 3765
Size: 559 34 1414 34 1724
Fragments arranged by size:
1724 1414 559 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1 3765
Size: 1 3764
BfrBI ATG'CAT
Cuts at: 0 791 3765
Size: 791 2974
BglI GCCn_nnn'nGGC
Cuts at: 0 2114 2698 2896 3765
Size: 2114 584 198 869
Fragments arranged by size:
2114 869 584 198
BlpI GC'TnA_GC
Cuts at: 0 3198 3765
Size: 3198 567
Bme1580I G_kGCm'C
Cuts at: 0 512 953 1312 2119 2447 2534 2645 3765
Size: 512 441 359 807 328 87 111 1120
Fragments arranged by size:
1120 807 512 441 359 328 111 87
BmgBI CAC'GTC
Cuts at: 0 507 1326 2507 3155 3612 3765
Size: 507 819 1181 648 457 153
Fragments arranged by size:
1181 819 648 507 457 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 1087 2505 2539 2720 3253 3765
Size: 26 1061 1418 34 181 533 512
Fragments arranged by size:
1418 1061 533 512 181 34 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1929 2841 3765
Size: 1929 912 924
Fragments arranged by size:
1929 924 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1419 1482 1619 1899 1916 2638 3007
Size: 96 1323 63 137 280 17 722 369
Cuts at: 3007 3032 3508 3765
Size: 25 476 257
Fragments arranged by size:
1323 722 476 369 280 257 137 96
63 25 17
Bpu10I CC'TnA_GC
Cuts at: 0 1846 3765
Size: 1846 1919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 3765
Size: 767 2998
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 2140 3765
Size: 118 2022 1625
Fragments arranged by size:
2022 1625 118
BsaHI Gr'CG_yC
Cuts at: 0 694 874 1990 2701 3100 3143 3254 3765
Size: 694 180 1116 711 399 43 111 511
Fragments arranged by size:
1116 711 694 511 399 180 111 43
BsaWI w'CCGG_w
Cuts at: 0 213 827 2344 2431 3765
Size: 213 614 1517 87 1334
Fragments arranged by size:
1517 1334 614 213 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 491 496 521 526 1417 1447 3506 3536
Size: 491 5 25 5 891 30 2059 30
Cuts at: 3536 3765
Size: 229
Fragments arranged by size:
2059 891 491 229 30 30 25 5
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 541 811 1366 1485 1548 1720 2224 3312
Size: 541 270 555 119 63 172 504 1088
Cuts at: 3312 3765
Size: 453
Fragments arranged by size:
1088 555 541 504 453 270 172 119
63
BseYI C'CCAG_C
Cuts at: 0 25 62 67 192 1369 1644 1827 1839
Size: 25 37 5 125 1177 275 183 12
Cuts at: 1839 2166 2523 2776 2848 3765
Size: 327 357 253 72 917
Fragments arranged by size:
1177 917 357 327 275 253 183 125
72 37 25 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1123 1329 1348 2028 2177 3765
Size: 780 343 206 19 680 149 1588
Fragments arranged by size:
1588 780 680 343 206 149 19
BsiHKAI G_wGCw'C
Cuts at: 0 554 953 1100 1385 1478 2431 3765
Size: 554 399 147 285 93 953 1334
Fragments arranged by size:
1334 953 554 399 285 147 93
BsmI GAATG_Cn'
Cuts at: 0 570 793 1373 2713 3765
Size: 570 223 580 1340 1052
Fragments arranged by size:
1340 1052 580 570 223
Bsp1286I G_dGCh'C
Cuts at: 0 53 62 512 554 953 1100 1312 1385
Size: 53 9 450 42 399 147 212 73
Cuts at: 1385 1478 2119 2156 2431 2447 2534 2645 3211
Size: 93 641 37 275 16 87 111 566
Cuts at: 3211 3235 3765
Size: 24 530
Fragments arranged by size:
641 566 530 450 399 275 212 147
111 93 87 73 53 42 37 24
16 9
BspEI T'CCGG_A
Cuts at: 0 827 2344 3765
Size: 827 1517 1421
Fragments arranged by size:
1517 1421 827
BspHI T'CATG_A
Cuts at: 0 1590 3440 3765
Size: 1590 1850 325
Fragments arranged by size:
1850 1590 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 1741 2765 2984 3765
Size: 429 1312 1024 219 781
Fragments arranged by size:
1312 1024 781 429 219
BsrBI CCG'CTC
Cuts at: 0 2937 3765
Size: 2937 828
BsrDI GCAATG_nn'
Cuts at: 0 1797 3765
Size: 1797 1968
BsrFI r'CCGG_y
Cuts at: 0 1400 3765
Size: 1400 2365
BsrGI T'GTAC_A
Cuts at: 0 614 3765
Size: 614 3151
BssSI C'ACGA_G
Cuts at: 0 838 1099 1471 3765
Size: 838 261 372 2294
Fragments arranged by size:
2294 838 372 261
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3558 3765
Size: 3558 207
BstBI TT'CG_AA
Cuts at: 0 307 3765
Size: 307 3458
BstEII G'GTnAC_C
Cuts at: 0 487 2492 3765
Size: 487 2005 1273
Fragments arranged by size:
2005 1273 487
BstXI CCAn_nnnn'nTGG
Cuts at: 0 402 681 3612 3765
Size: 402 279 2931 153
Fragments arranged by size:
2931 402 279 153
BstYI r'GATC_y
Cuts at: 0 608 1151 1394 2293 2434 3399 3730 3765
Size: 608 543 243 899 141 965 331 35
Fragments arranged by size:
965 899 608 543 331 243 141 35
Bsu36I CC'TnA_GG
Cuts at: 0 424 1073 2999 3765
Size: 424 649 1926 766
Fragments arranged by size:
1926 766 649 424
BtgI C'CryG_G
Cuts at: 0 53 143 521 1258 1670 2645 2915 3017
Size: 53 90 378 737 412 975 270 102
Cuts at: 3017 3765
Size: 748
Fragments arranged by size:
975 748 737 412 378 270 102 90
53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 683 3765
Size: 683 3082
BtsI GCAGTG_nn'
Cuts at: 0 79 111 171 768 1963 2268 2563 3765
Size: 79 32 60 597 1195 305 295 1202
Fragments arranged by size:
1202 1195 597 305 295 79 60 32
DraIII CAC_nnn'GTG
Cuts at: 0 558 705 3765
Size: 558 147 3060
Fragments arranged by size:
3060 558 147
DrdI GACnn_nn'nnGTC
Cuts at: 0 3436 3765
Size: 3436 329
EaeI y'GGCC_r
Cuts at: 0 404 584 1136 1512 2912 3765
Size: 404 180 552 376 1400 853
Fragments arranged by size:
1400 853 552 404 376 180
EarI CTCTTCn'nnn_
Cuts at: 0 449 1804 3749 3765
Size: 449 1355 1945 16
Fragments arranged by size:
1945 1355 449 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 11 2442 3073 3765
Size: 11 2431 631 692
Fragments arranged by size:
2431 692 631 11
EcoICRI GAG'CTC
Cuts at: 0 1383 3765
Size: 1383 2382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 373 648 1419 1482 1619 1828 1899
Size: 96 277 275 771 63 137 209 71
Cuts at: 1899 1916 2638 3007 3032 3508 3526 3765
Size: 17 722 369 25 476 18 239
Fragments arranged by size:
771 722 476 369 277 275 239 209
137 96 71 63 25 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 886 1072 2176 2761 3765
Size: 886 186 1104 585 1004
Fragments arranged by size:
1104 1004 886 585 186
EcoO109I rG'GnC_Cy
Cuts at: 0 75 1077 1124 1309 1359 1466 2116 2443
Size: 75 1002 47 185 50 107 650 327
Cuts at: 2443 2747 2924 3189 3765
Size: 304 177 265 576
Fragments arranged by size:
1002 650 576 327 304 265 185 177
107 75 50 47
EcoRI G'AATT_C
Cuts at: 0 974 3765
Size: 974 2791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 762 2172 2357 3765
Size: 762 1410 185 1408
Fragments arranged by size:
1410 1408 762 185
HaeII r_GCGC'y
Cuts at: 0 697 1846 2584 2704 3103 3765
Size: 697 1149 738 120 399 662
Fragments arranged by size:
1149 738 697 662 399 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 787 819 1205 1237 3106 3138
Size: 496 32 259 32 386 32 1869 32
Cuts at: 3138 3181 3213 3765
Size: 43 32 552
Fragments arranged by size:
1869 552 496 386 259 43 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 739 3765
Size: 739 3026
KasI G'GCGC_C
Cuts at: 0 693 2700 3099 3765
Size: 693 2007 399 666
Fragments arranged by size:
2007 693 666 399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 3686 3765
Size: 1208 2478 79
Fragments arranged by size:
2478 1208 79
MscI TGG'CCA
Cuts at: 0 406 1138 3765
Size: 406 732 2627
Fragments arranged by size:
2627 732 406
MslI CAynn'nnrTG
Cuts at: 0 494 842 1115 3358 3610 3765
Size: 494 348 273 2243 252 155
Fragments arranged by size:
2243 494 348 273 252 155
MspA1I CmG'CkG
Cuts at: 0 468 1238 1430 1648 1754 2466 2519 2985
Size: 468 770 192 218 106 712 53 466
Cuts at: 2985 3049 3456 3765
Size: 64 407 309
Fragments arranged by size:
770 712 468 466 407 309 218 192
106 64 53
NarI GG'CG_CC
Cuts at: 0 694 2701 3100 3765
Size: 694 2007 399 665
Fragments arranged by size:
2007 694 665 399
NcoI C'CATG_G
Cuts at: 0 1258 1670 2915 3017 3765
Size: 1258 412 1245 102 748
Fragments arranged by size:
1258 1245 748 412 102
NdeI CA'TA_TG
Cuts at: 0 3666 3765
Size: 3666 99
NsiI A_TGCA'T
Cuts at: 0 793 3765
Size: 793 2972
NspI r_CATG'y
Cuts at: 0 759 3765
Size: 759 3006
PasI CC'CwG_GG
Cuts at: 0 1882 3765
Size: 1882 1883
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1894 2489 3765
Size: 1035 859 595 1276
Fragments arranged by size:
1276 1035 859 595
PfoI T'CCnGG_A
Cuts at: 0 1028 3765
Size: 1028 2737
PpuMI rG'GwC_Cy
Cuts at: 0 75 1077 1466 2747 3189 3765
Size: 75 1002 389 1281 442 576
Fragments arranged by size:
1281 1002 576 442 389 75
PshAI GACnn'nnGTC
Cuts at: 0 3287 3765
Size: 3287 478
PspOMI G'GGCC_C
Cuts at: 0 1308 2115 2443 3765
Size: 1308 807 328 1322
Fragments arranged by size:
1322 1308 807 328
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 3765
Size: 1201 32 2532
Fragments arranged by size:
2532 1201 32
PstI C_TGCA'G
Cuts at: 0 86 292 372 1160 1415 2228 2969 2984
Size: 86 206 80 788 255 813 741 15
Cuts at: 2984 3398 3765
Size: 414 367
Fragments arranged by size:
813 788 741 414 367 255 206 86
80 15
PvuII CAG'CTG
Cuts at: 0 468 1238 1648 1754 2519 2985 3049 3456
Size: 468 770 410 106 765 466 64 407
Cuts at: 3456 3765
Size: 309
Fragments arranged by size:
770 765 468 466 410 407 309 106
64
SacI G_AGCT'C
Cuts at: 0 1385 3765
Size: 1385 2380
SanDI GG'GwC_CC
Cuts at: 0 1466 2747 3765
Size: 1466 1281 1018
Fragments arranged by size:
1466 1281 1018
SapI GCTCTTCn'nnn_
Cuts at: 0 3749 3765
Size: 3749 16
SbfI CC_TGCA'GG
Cuts at: 0 3398 3765
Size: 3398 367
SexAI A'CCwGG_T
Cuts at: 0 626 881 3765
Size: 626 255 2884
Fragments arranged by size:
2884 626 255
SfcI C'TryA_G
Cuts at: 0 82 288 368 1156 1411 2013 2224 2965
Size: 82 206 80 788 255 602 211 741
Cuts at: 2965 2980 3050 3394 3514 3765
Size: 15 70 344 120 251
Fragments arranged by size:
788 741 602 344 255 251 211 206
120 82 80 70 15
SfoI GGC'GCC
Cuts at: 0 695 2702 3101 3765
Size: 695 2007 399 664
Fragments arranged by size:
2007 695 664 399
SmaI CCC'GGG
Cuts at: 0 2896 3765
Size: 2896 869
SmlI C'TyrA_G
Cuts at: 0 782 2411 3765
Size: 782 1629 1354
Fragments arranged by size:
1629 1354 782
SspI AAT'ATT
Cuts at: 0 3304 3654 3765
Size: 3304 350 111
Fragments arranged by size:
3304 350 111
StuI AGG'CCT
Cuts at: 0 2767 3765
Size: 2767 998
StyI C'CwwG_G
Cuts at: 0 44 383 1258 1670 2177 2297 2359 2915
Size: 44 339 875 412 507 120 62 556
Cuts at: 2915 3017 3765
Size: 102 748
Fragments arranged by size:
875 748 556 507 412 339 120 102
62 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1021 3765
Size: 1021 2744
TatI w'GTAC_w
Cuts at: 0 614 1217 3765
Size: 614 603 2548
Fragments arranged by size:
2548 614 603
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1417 1449 3765
Size: 491 32 894 32 2316
Fragments arranged by size:
2316 894 491 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1765 3765
Size: 1765 2000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 2031 3765
Size: 51 1980 1734
Fragments arranged by size:
1980 1734 51
XhoI C'TCGA_G
Cuts at: 0 2411 3765
Size: 2411 1354
XmaI C'CCGG_G
Cuts at: 0 2894 3765
Size: 2894 871
XmnI GAAnn'nnTTC
Cuts at: 0 805 2340 2352 3677 3765
Size: 805 1535 12 1325 88
Fragments arranged by size:
1535 1325 805 88 12
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PsrI PstI PvuII
SacI SanDI SapI SbfI SexAI SfcI SfoI SmaI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BbvCI BclI BglII
BmtI BsaAI BsaBI BsiEI BsiWI BsmBI BssHII BstZ17I
ClaI CspCI DraI EagI EcoRV FseI FspI FspAI
HincII HpaI KpnI MfeI MluI NaeI NgoMIV NheI
NotI NruI PacI PciI PmeI PmlI PpiI PsiI
PspXI PvuI RsrII SacII SalI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI XbaI ZraI