(Circular) (Six-base) MAPSORT of: pFN21AB6394.seq Check: 4438 from: 1 to: 5614 pFN21AB6394 5614 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2522 2522 Size: 5614 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2405 2824 2935 2405 Size: 419 111 5084 Fragments arranged by size: 5084 419 111 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3677 5061 5329 1632 Size: 2045 1384 268 1917 Fragments arranged by size: 2045 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 2415 4513 5490 1203 Size: 1212 2098 977 1327 Fragments arranged by size: 2098 1327 1212 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2630 2662 2630 Size: 32 5582 AlwNI CAG_nnn'CTG Cuts at: 2439 3357 4929 2439 Size: 918 1572 3124 Fragments arranged by size: 3124 1572 918 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2923 3149 3301 5410 1493 Size: 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2939 1870 Size: 73 996 4545 Fragments arranged by size: 4545 996 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2405 2824 2935 4390 Size: 325 217 627 251 367 419 111 1455 Cuts at: 4390 618 Size: 1842 Fragments arranged by size: 1842 1455 627 419 367 325 251 217 111 BanII G_rGCy'C Cuts at: 1391 2118 2933 1391 Size: 727 815 4072 Fragments arranged by size: 4072 815 727 BbeI G_GCGC'C Cuts at: 2042 2042 Size: 5614 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 4225 136 Size: 107 122 71 631 605 343 2210 1525 Fragments arranged by size: 2210 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5609 5609 Size: 5614 BlpI GC'TnA_GC Cuts at: 2907 3031 2907 Size: 124 5490 Bme1580I G_kGCm'C Cuts at: 1790 3675 4831 1790 Size: 1885 1156 2573 Fragments arranged by size: 2573 1885 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4303 442 Size: 416 53 83 399 24 1928 958 1753 Fragments arranged by size: 1928 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 2740 2737 Size: 3 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2538 2628 4274 1239 Size: 902 397 90 1646 2579 Fragments arranged by size: 2579 1646 902 397 90 Bpu10I CC'TnA_GC Cuts at: 2019 2019 Size: 5614 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2408 3739 4604 4902 2408 Size: 1331 865 298 3120 Fragments arranged by size: 3120 1331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2384 2725 2882 4277 882 Size: 617 885 341 157 1395 2219 Fragments arranged by size: 2219 1395 885 617 341 157 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 3421 3803 4438 Size: 53 83 186 936 506 1382 382 635 Cuts at: 4438 275 Size: 1451 Fragments arranged by size: 1451 1382 936 635 506 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 4047 4445 4719 4866 5277 2247 Size: 1800 398 274 147 411 2584 Fragments arranged by size: 2584 1800 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2273 2343 2713 2729 1186 Size: 1084 3 70 370 16 4071 Fragments arranged by size: 4071 1084 370 70 16 3 BseYI C'CCAG_C Cuts at: 2125 4817 5313 2125 Size: 2692 496 2426 Fragments arranged by size: 2692 2426 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5041 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3675 3760 4831 2118 Size: 1557 85 1071 2901 Fragments arranged by size: 2901 1557 1071 85 BsmI GAATG_Cn' Cuts at: 2512 3125 3218 2512 Size: 613 93 4908 Fragments arranged by size: 4908 613 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2933 3675 3760 4831 1391 Size: 399 328 815 742 85 1071 2174 Fragments arranged by size: 2174 1071 815 742 399 328 85 BspEI T'CCGG_A Cuts at: 2247 5277 2247 Size: 3030 2584 BspHI T'CATG_A Cuts at: 2833 3503 5233 2833 Size: 670 1730 3214 Fragments arranged by size: 3214 1730 670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 2969 844 Size: 1678 447 3489 Fragments arranged by size: 3489 1678 447 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2142 4109 4283 65 Size: 1183 894 1967 174 1396 Fragments arranged by size: 1967 1396 1183 894 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 4258 4445 1700 Size: 110 265 2183 187 2869 Fragments arranged by size: 2869 2183 265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5614 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 2220 3674 4686 2220 Size: 1454 1012 3148 Fragments arranged by size: 3148 1454 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2383 794 Size: 1589 4025 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5614 BstYI r'GATC_y Cuts at: 1972 2459 2944 3311 3696 3713 5165 5251 5263 Size: 487 485 367 385 17 1452 86 12 Cuts at: 5263 5609 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 487 485 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2712 2712 Size: 5614 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 513 Size: 552 939 81 4042 Fragments arranged by size: 4042 939 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 2584 3130 3924 3944 2584 Size: 546 794 20 4254 Fragments arranged by size: 4254 794 546 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DraIII CAC_nnn'GTG Cuts at: 2383 2383 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 3061 3950 Size: 54 1425 50 275 18 234 997 889 Cuts at: 3950 5579 8 Size: 1629 43 Fragments arranged by size: 1629 1425 997 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3544 1353 Size: 2191 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4201 4575 4721 387 Size: 1064 138 2612 374 146 1280 Fragments arranged by size: 2612 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2116 2116 Size: 5614 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2538 2628 3677 4274 5061 5329 Size: 393 509 397 90 1049 597 787 268 Cuts at: 5329 1239 Size: 1524 Fragments arranged by size: 1524 1049 787 597 509 397 393 268 90 EcoO109I rG'GnC_Cy Cuts at: 2860 2860 Size: 5614 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 2908 Size: 5614 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 2042 4761 1678 Size: 364 2719 2531 Fragments arranged by size: 2719 2531 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2448 2451 2480 2483 4255 Size: 32 135 32 876 3 29 3 1772 Cuts at: 4255 4287 4329 4361 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1772 876 135 42 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 2659 748 Size: 1911 3703 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KasI G'GCGC_C Cuts at: 2038 2038 Size: 5614 KpnI G_GTAC'C Cuts at: 2409 2828 2939 2409 Size: 419 111 5084 Fragments arranged by size: 5084 419 111 MfeI C'AATT_G Cuts at: 2593 3213 2593 Size: 620 4994 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 4728 4912 1305 Size: 834 2589 184 2007 Fragments arranged by size: 2589 2007 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 10 Size: 54 1425 343 234 3558 Fragments arranged by size: 3558 1425 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2817 3572 3931 4090 518 Size: 648 1651 755 359 159 2042 Fragments arranged by size: 2042 1651 755 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3030 3707 4855 5100 1606 Size: 1424 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 677 245 NarI GG'CG_CC Cuts at: 2039 2039 Size: 5614 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4123 Fragments arranged by size: 4123 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2419 2972 4517 1132 Size: 97 1190 553 1545 2229 Fragments arranged by size: 2229 1545 1190 553 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 2415 4513 2415 Size: 2098 3516 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5614 PfoI T'CCnGG_A Cuts at: 2118 2438 2118 Size: 320 5294 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2860 2860 Size: 5614 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PstI C_TGCA'G Cuts at: 830 2055 2193 2579 2755 2966 830 Size: 1225 138 386 176 211 3478 Fragments arranged by size: 3478 1225 386 211 176 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 SacI G_AGCT'C Cuts at: 2118 2118 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2051 2189 2575 2751 2962 4097 4778 Size: 219 1006 138 386 176 211 1135 681 Cuts at: 4778 4969 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1006 681 386 219 211 191 176 138 SfoI GGC'GCC Cuts at: 2040 2040 Size: 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 2075 1700 Size: 375 5239 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2387 3718 4619 4881 820 Size: 197 926 444 1331 901 262 1553 Fragments arranged by size: 1553 1331 926 901 444 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2004 2847 3053 513 Size: 552 939 843 206 3074 Fragments arranged by size: 3074 939 843 552 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3641 3658 3811 3996 4441 325 Size: 1255 2061 17 153 185 445 1498 Fragments arranged by size: 2061 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3860 5572 96 Size: 274 80 33 51 1443 1883 1712 138 Fragments arranged by size: 1883 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2581 2613 2763 2795 5574 5606 2581 Size: 32 150 32 2779 32 2589 Fragments arranged by size: 2779 2589 150 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI