################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 958 GTAGAC GT^[AC][GT]AC AciI 1226 GCGG CCGC(-3/-1) AcsI 237 GAATTC [GA]^AATT[CT] AflIII 977 ACATGT A^C[GA][CT]GT ApaI 1505 GGGCCC GGGCC^C ApoI 237 GAATTC [GA]^AATT[CT] AvaI 20 CCCGAG C^[CT]CG[GA]G BanII 1505 GGGCCC G[GA]GC[CT]^C BbvI 428 GCAGC GCAGC(8/12) BclI 531 TGATCA T^GATCA BmyI 1505 GGGCCC G[AGT]GC[ACT]^C BseRI 832 GAGGAG GAGGAG(10/8) BsmBI 967 GAGACG CGTCTC(1/5) BsoBI 20 CCCGAG C^[CT]CG[GA]G Bsp1286I 1505 GGGCCC G[AGT]GC[ACT]^C BspHI 900 TCATGA T^CATGA BspLU11I 977 ACATGT A^CATGT BspMI 207 GCAGGT ACCTGC(4/8) BsrDI 749 GCAATG GCAATG(2/0) BssSI 412 CTCGTG CACGAG(-5/-1) BstYI 216 GGATCT [GA]^GATC[CT] EcoRI 237 GAATTC G^AATTC EcoT14I 456 CCAAGG C^C[AT][AT]GG FbaI 531 TGATCA T^GATCA Fnu4HI 428 GCAGC GC^[ACGT]GC HgaI 654 GCGTC GACGC(5/10) HincII 871 GTCAAC GT[CT]^[GA]AC HindII 871 GTCAAC GT[CT]^[GA]AC ItaI 428 GCAGC GC^[ACGT]GC MflI 216 GGATCT [GA]^GATC[CT] MseI 1174 TTAA T^TAA NdeI 202 CATATG CA^TATG NspI 977 ACATGT [GA]CATG^[CT] PshAI 649 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1134 CTGCAG CTGCA^G RcaI 900 TCATGA T^CATGA SexAI 98 ACCTGGT A^CC[AT]GGT SfaNI 568 GATGC GCATC(5/9) SpeI 252 ACTAGT A^CTAGT StuI 1373 AGGCCT AGG^CCT StyI 456 CCAAGG C^C[AT][AT]GG Tru9I 1174 TTAA T^TAA TseI 428 GCAGC G^C[AT]GC XhoII 216 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BcnI 105 CCGGG CC^[GC]GG 398 CCCGG Bsp1407I 473 TGTACA T^GTACA 980 TGTACA BsrGI 473 TGTACA T^GTACA 980 TGTACA BsrI 987 ACTGG ACTGG(1/-1) 695 CCAGT BstXI 1070 CCACTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1329 CCAGAGCAGTGG HapII 105 CCGG C^CGG 399 CCGG HpaII 105 CCGG C^CGG 399 CCGG MspI 105 CCGG C^CGG 399 CCGG NciI 105 CCGGG CC^[GC]GG 398 CCCGG SfcI 1103 CTACAG C^T[GA][CT]AG 1134 CTGCAG SmlI 494 CTCAAG C^T[CT][GA]AG 1086 CTCAAG SspBI 473 TGTACA T^GTACA 980 TGTACA Tsp45I 244 GTGAC ^GT[GC]AC 1348 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI AlwI AvaII BfaI BpmI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI Tsp509I TspRI