################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AclI AgeI Alw44I ApaLI AscI AviII AvrII BlnI BsiWI BspDI BspLU11I BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EcoT22I FseI FspI HindIII MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I RsrII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 3037 GACGTC GACGT^C Acc65I 2533 GGTACC G^GTACC AflII 1698 CTTAAG C^TTAAG AflIII 4810 ACACGT A^C[GA][CT]GT Asp718I 2533 GGTACC G^GTACC AspI 8282 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1586 GGATCC G^GATCC BbrPI 4811 CACGTG CAC^GTG BciVI 3131 GTATCC GTATCC(6/5) BfrI 1698 CTTAAG C^TTAAG BsaAI 4811 CACGTG [CT]AC^GT[GA] DraI 5404 TTTAAA TTT^AAA EagI 2430 CGGCCG C^GGCCG EclXI 2430 CGGCCG C^GGCCG Eco52I 2430 CGGCCG C^GGCCG EcoRV 6889 GATATC GAT^ATC HpaI 1281 GTTAAC GTT^AAC KpnI 2533 GGTACC GGTAC^C KspI 2319 CCGCGG CCGC^GG NcoI 5114 CCATGG C^CATGG NheI 5104 GCTAGC G^CTAGC PflFI 8282 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2203 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4811 CACGTG CAC^GTG PmlI 4811 CACGTG CAC^GTG PshAI 5940 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 6243 CGATCG CGAT^CG SacII 2319 CCGCGG CCGC^GG SbfI 66 CCTGCAGG CCTGCA^GG ScaI 2600 AGTACT AGT^ACT SphI 7466 GCATGC GCATG^C Sse8387I 66 CCTGCAGG CCTGCA^GG SspI 8452 AATATT AAT^ATT Tth111I 8282 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2203 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1686 GTCTAC GT^[AC][GT]AC 3760 GTAGAC AccII 2320 CGCG CG^CG 6164 CGCG AccIII 1589 TCCGGA T^CCGGA 1979 TCCGGA Asp700I 1954 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5608 GAAATGCTTC BbeI 6081 GGCGCC GGCGC^C 8026 GGCGCC BclI 177 TGATCA T^GATCA 518 TGATCA BglII 200 AGATCT A^GATCT 1302 AGATCT BsaBI 3917 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7793 GATCGAAATC BsaWI 1589 TCCGGA [AT]^CCGG[AT] 1979 TCCGGA BseAI 1589 TCCGGA T^CCGGA 1979 TCCGGA BsiEI 2430 CGGCCG CG[GA][CT]^CG 6243 CGATCG BspEI 1589 TCCGGA T^CCGGA 1979 TCCGGA BspHI 4590 TCATGA T^CATGA 7259 TCATGA BsrDI 911 GCAATG GCAATG(2/0) 1405 GCAATG BstUI 2320 CGCG CG^CG 6164 CGCG DraIII 1672 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 7951 CACAGGGTG FbaI 177 TGATCA T^GATCA 518 TGATCA KasI 6081 GGCGCC G^GCGCC 8026 GGCGCC MamI 3917 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7793 GATCGAAATC MroI 1589 TCCGGA T^CCGGA 1979 TCCGGA MvnI 2320 CGCG CG^CG 6164 CGCG NaeI 2432 GCCGGC GCC^GGC 7219 GCCGGC NarI 6081 GGCGCC GG^CGCC 8026 GGCGCC NgoMIV 2432 GCCGGC G^CCGGC 7219 GCCGGC PaeR7I 3468 CTCGAG C^TCGAG 4833 CTCGAG RcaI 4590 TCATGA T^CATGA 7259 TCATGA SfoI 6081 GGCGCC GGC^GCC 8026 GGCGCC XbaI 840 TCTAGA T^CTAGA 4570 TCTAGA XhoI 3468 CTCGAG C^TCGAG 4833 CTCGAG XmnI 1954 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5608 GAAATGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AseI AsnI AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI