(Circular) (Six-base) MAPSORT of: pFN21AA0300.seq Check: 9736 from: 1 to: 13222 pFN21AA0300 13222 bp With 182 enzymes: SgfI * December 26, 2011 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2046 4801 2046 Size: 2755 10467 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5046 11032 278 Size: 53 83 186 936 3510 5986 2468 Fragments arranged by size: 5986 3510 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 5766 10565 1937 Size: 1755 2074 4799 4594 Fragments arranged by size: 4799 4594 2074 1755 Acc65I G'GTAC_C Cuts at: 4538 10543 4538 Size: 6005 7217 AclI AA'CG_TT Cuts at: 1563 11349 11722 1563 Size: 9786 373 3063 Fragments arranged by size: 9786 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 6723 6960 7267 7679 8316 10088 10286 Size: 1086 4005 237 307 412 637 1772 198 Cuts at: 10286 10503 11285 12669 12937 1632 Size: 217 782 1384 268 1917 Fragments arranged by size: 4005 1917 1772 1384 1086 782 637 412 307 268 237 217 198 AfeI AGC'GCT Cuts at: 2716 3071 7108 2716 Size: 355 4037 8830 Fragments arranged by size: 8830 4037 355 AflII C'TTAA_G Cuts at: 820 1017 3703 820 Size: 197 2686 10339 Fragments arranged by size: 10339 2686 197 AflIII A'CryG_T Cuts at: 1203 6815 12121 13098 1203 Size: 5612 5306 977 1327 Fragments arranged by size: 5612 5306 1327 977 AgeI A'CCGG_T Cuts at: 12053 12053 Size: 13222 AhdI GACnn_n'nnGTC Cuts at: 6691 6733 7629 11951 6691 Size: 42 896 4322 7962 Fragments arranged by size: 7962 4322 896 42 AleI CACnn'nnGTG Cuts at: 1166 3098 9159 1166 Size: 1932 6061 5229 Fragments arranged by size: 6061 5229 1932 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 9251 10116 10464 1184 Size: 8067 865 348 3942 Fragments arranged by size: 8067 3942 865 348 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5302 5334 8123 8155 5302 Size: 32 2789 32 10369 Fragments arranged by size: 10369 2789 32 32 AlwNI CAG_nnn'CTG Cuts at: 2059 2128 2249 3646 6155 7928 8312 9457 9621 Size: 69 121 1397 2509 1773 384 1145 164 Cuts at: 9621 9703 10965 12537 2059 Size: 82 1262 1572 2744 Fragments arranged by size: 2744 2509 1773 1572 1397 1262 1145 384 164 121 82 69 ApaI G_GGCC'C Cuts at: 4167 4969 8621 10332 4167 Size: 802 3652 1711 7057 Fragments arranged by size: 7057 3652 1711 802 ApaLI G'TGCA_C Cuts at: 11279 12435 11279 Size: 1156 12066 ApoI r'AATT_y Cuts at: 1493 2883 3039 6580 9354 10401 10513 10531 10757 Size: 1390 156 3541 2774 1047 112 18 226 Cuts at: 10757 10909 13018 1493 Size: 152 2109 1697 Fragments arranged by size: 3541 2774 2109 1697 1390 1047 226 156 152 112 18 AseI AT'TA_AT Cuts at: 160 1032 6525 7257 10413 10496 11776 160 Size: 872 5493 732 3156 83 1280 1606 Fragments arranged by size: 5493 3156 1606 1280 872 732 83 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13222 AvaI C'yCGr_G Cuts at: 1870 1943 3612 4675 4972 5005 5473 5878 6235 Size: 73 1669 1063 297 33 468 405 357 Cuts at: 6235 6250 6430 6451 6838 8586 8623 8742 8780 Size: 15 180 21 387 1748 37 119 38 Cuts at: 8780 9599 10547 1870 Size: 819 948 4545 Fragments arranged by size: 4545 1748 1669 1063 948 819 468 405 387 357 297 180 119 73 38 37 33 21 15 BamHI G'GATC_C Cuts at: 3591 10552 10919 3591 Size: 6961 367 5894 Fragments arranged by size: 6961 5894 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3113 4229 4440 4538 5098 Size: 325 217 627 1326 1116 211 98 560 Cuts at: 5098 6169 6473 8086 8593 9227 10031 10543 11998 Size: 1071 304 1613 507 634 804 512 1455 Cuts at: 11998 618 Size: 1842 Fragments arranged by size: 1842 1613 1455 1326 1116 1071 804 634 627 560 512 507 325 304 217 211 98 BanII G_rGCy'C Cuts at: 1391 3189 4031 4167 4800 4969 5213 5315 5351 Size: 1798 842 136 633 169 244 102 36 Cuts at: 5351 6067 6272 6410 7022 8621 9098 9317 10332 Size: 716 205 138 612 1599 477 219 1015 Cuts at: 10332 10437 10541 1391 Size: 105 104 4072 Fragments arranged by size: 4072 1798 1599 1015 842 716 633 612 477 244 219 205 169 138 136 105 104 102 36 BbeI G_GCGC'C Cuts at: 8090 10035 8090 Size: 1945 11277 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2954 3360 3898 4160 6324 6606 8798 Size: 971 1055 406 538 262 2164 282 2192 Cuts at: 8798 9114 10274 928 Size: 316 1160 3876 Fragments arranged by size: 3876 2192 2164 1160 1055 971 538 406 316 282 262 BbvCI CC'TCA_GC Cuts at: 2375 3450 5403 6669 6883 7560 2375 Size: 1075 1953 1266 214 677 8037 Fragments arranged by size: 8037 1953 1266 1075 677 214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5765 5799 8943 8977 9508 Size: 34 50 34 3744 34 3144 34 531 Cuts at: 9508 9542 11413 11447 1903 Size: 34 1871 34 3678 Fragments arranged by size: 3744 3678 3144 1871 531 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5147 11114 12324 5147 Size: 5967 1210 6045 Fragments arranged by size: 6045 5967 1210 BclI T'GATC_A Cuts at: 1548 2182 2523 1548 Size: 634 341 12247 Fragments arranged by size: 12247 634 341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2690 5096 7677 Size: 107 122 71 631 605 1018 2406 2581 Cuts at: 7677 8462 10040 11833 136 Size: 785 1578 1793 1525 Fragments arranged by size: 2581 2406 1793 1578 1525 1018 785 631 605 122 107 71 BglII A'GATC_T Cuts at: 2205 3307 13217 2205 Size: 1102 9910 2210 Fragments arranged by size: 9910 2210 1102 BlpI GC'TnA_GC Cuts at: 2490 7114 7667 10048 10639 2490 Size: 4624 553 2381 591 5073 Fragments arranged by size: 5073 4624 2381 591 553 Bme1580I G_kGCm'C Cuts at: 1790 4167 4969 6172 6243 6478 8598 8621 10332 Size: 2377 802 1203 71 235 2120 23 1711 Cuts at: 10332 11283 12439 1790 Size: 951 1156 2573 Fragments arranged by size: 2573 2377 2120 1711 1203 1156 951 802 235 71 23 BmgBI CAC'GTC Cuts at: 1318 4695 1318 Size: 3377 9845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4597 5310 5316 Size: 416 53 83 399 24 3180 713 6 Cuts at: 5316 5409 7861 8680 9660 10953 11911 442 Size: 93 2452 819 980 1293 958 1753 Fragments arranged by size: 3180 2452 1753 1293 980 958 819 713 416 399 93 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 7113 1056 Size: 6057 7165 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 4575 6053 6864 7036 9523 2449 Size: 2126 1478 811 172 2487 6148 Fragments arranged by size: 6148 2487 2126 1478 811 172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2656 2774 3831 4272 4395 5335 5586 5829 Size: 1417 118 1057 441 123 940 251 243 Cuts at: 5829 6009 6051 6161 6417 9434 9566 9603 10229 Size: 180 42 110 256 3017 132 37 626 Cuts at: 10229 10413 11882 1239 Size: 184 1469 2579 Fragments arranged by size: 3017 2579 1469 1417 1057 940 626 441 256 251 243 184 180 132 123 118 110 42 37 Bpu10I CC'TnA_GC Cuts at: 2375 3450 4319 4534 5403 6372 6669 6883 7560 Size: 1075 869 215 869 969 297 214 677 Cuts at: 7560 7983 9895 2375 Size: 423 1912 5702 Fragments arranged by size: 5702 1912 1075 969 869 869 677 423 297 215 214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2807 3081 3491 3560 4417 6148 7658 8677 9214 Size: 274 410 69 857 1731 1510 1019 537 Cuts at: 9214 9487 11347 12212 12510 2807 Size: 273 1860 865 298 3519 Fragments arranged by size: 3519 1860 1731 1510 1019 865 857 537 410 298 274 273 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3613 5847 6024 6330 9293 11885 882 Size: 617 2114 2234 177 306 2963 2592 2219 Fragments arranged by size: 2963 2592 2234 2219 2114 617 306 177 BsaAI yAC'GTr Cuts at: 493 1204 6818 11013 493 Size: 711 5614 4195 2702 Fragments arranged by size: 5614 4195 2702 711 BsaBI GATnn'nnATC Cuts at: 5926 9802 13216 5926 Size: 3876 3414 5932 Fragments arranged by size: 5932 3876 3414 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5043 8087 8507 10032 Size: 53 83 186 936 3510 3044 420 1525 Cuts at: 10032 11029 11411 12046 275 Size: 997 382 635 1451 Fragments arranged by size: 3510 3044 1525 1451 997 936 635 420 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3594 3984 11655 12053 12327 12474 12885 3594 Size: 390 7671 398 274 147 411 3931 Fragments arranged by size: 7671 3931 411 398 390 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2451 2481 2884 2914 3618 Size: 30 165 30 1048 30 403 30 704 Cuts at: 3618 3648 4842 4872 5302 5332 6593 6623 7299 Size: 30 1194 30 430 30 1261 30 676 Cuts at: 7299 7329 7791 7821 1178 Size: 30 462 30 6579 Fragments arranged by size: 6579 1261 1194 1048 704 676 462 430 403 165 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2892 3269 4715 4862 5144 5689 6436 Size: 850 856 377 1446 147 282 545 747 Cuts at: 6436 6469 6732 7462 7548 8503 8506 8509 1186 Size: 33 263 730 86 955 3 3 5899 Fragments arranged by size: 5899 1446 955 856 850 747 730 545 377 282 263 147 86 33 3 3 BseYI C'CCAG_C Cuts at: 2233 2389 2617 2741 3034 4031 4718 5325 5665 Size: 156 228 124 293 997 687 607 340 Cuts at: 5665 5750 5798 6501 6993 7727 8810 10277 10282 Size: 85 48 703 492 734 1083 1467 5 Cuts at: 10282 12425 12921 2233 Size: 2143 496 2534 Fragments arranged by size: 2534 2143 1467 1083 997 734 703 687 607 496 492 340 293 228 156 124 85 48 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 3257 7101 10061 10076 10508 1163 Size: 573 282 1239 3844 2960 15 432 3877 Fragments arranged by size: 3877 3844 2960 1239 573 432 282 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4438 8251 10672 11433 12461 1540 Size: 275 186 2437 3813 2421 761 1028 2301 Fragments arranged by size: 3813 2437 2421 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5608 6067 6373 6410 7022 9098 9531 11283 11368 Size: 459 306 37 612 2076 433 1752 85 Cuts at: 11368 12439 5608 Size: 1071 6391 Fragments arranged by size: 6391 2076 1752 1071 612 459 433 306 85 37 BsmI GAATG_Cn' Cuts at: 3825 7720 8607 10733 10826 3825 Size: 3895 887 2126 93 6221 Fragments arranged by size: 6221 3895 2126 887 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6648 8258 8906 9178 6648 Size: 1610 648 272 10692 Fragments arranged by size: 10692 1610 648 272 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3189 4031 4167 4800 4969 5213 5315 Size: 399 1399 842 136 633 169 244 102 Cuts at: 5315 5351 5608 6067 6172 6243 6272 6373 6410 Size: 36 257 459 105 71 29 101 37 Cuts at: 6410 6478 7022 8598 8621 9098 9317 9531 10332 Size: 68 544 1576 23 477 219 214 801 Cuts at: 10332 10437 10541 11283 11368 12439 1391 Size: 105 104 742 85 1071 2174 Fragments arranged by size: 2174 1576 1399 1071 842 801 742 633 544 477 459 399 257 244 219 214 169 136 105 105 104 102 101 85 71 68 37 36 29 23 BspEI T'CCGG_A Cuts at: 3594 3984 12885 3594 Size: 390 8901 3931 Fragments arranged by size: 8901 3931 390 BspHI T'CATG_A Cuts at: 6595 9264 11111 12841 6595 Size: 2669 1847 1730 6976 Fragments arranged by size: 6976 2669 1847 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 4801 5652 7224 7992 10577 844 Size: 1202 2755 851 1572 768 2585 3489 Fragments arranged by size: 3489 2755 2585 1572 1202 851 768 BsrBI CCG'CTC Cuts at: 1394 1823 3119 3699 5738 5981 9441 11109 1394 Size: 429 1296 580 2039 243 3460 1668 3507 Fragments arranged by size: 3507 3460 2039 1668 1296 580 429 243 BsrDI GCAATG_nn' Cuts at: 65 1248 2923 3417 11717 11891 65 Size: 1183 1675 494 8300 174 1396 Fragments arranged by size: 8300 1675 1396 1183 494 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4437 4652 8107 9051 9224 11866 12053 Size: 110 2627 215 3455 944 173 2642 187 Cuts at: 12053 1700 Size: 2869 Fragments arranged by size: 3455 2869 2642 2627 944 215 187 173 110 BsrGI T'GTAC_A Cuts at: 96 3529 3771 10237 10350 96 Size: 3433 242 6466 113 2968 Fragments arranged by size: 6466 3433 2968 242 113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13222 BssSI C'ACGA_G Cuts at: 3194 4766 6711 7075 7823 7933 10106 11282 12294 Size: 1572 1945 364 748 110 2173 1176 1012 Cuts at: 12294 3194 Size: 4122 Fragments arranged by size: 4122 2173 1945 1572 1176 1012 748 364 110 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 13222 BstBI TT'CG_AA Cuts at: 12026 12026 Size: 13222 BstEII G'GTnAC_C Cuts at: 1184 2621 6071 8714 9564 1184 Size: 1437 3450 2643 850 4842 Fragments arranged by size: 4842 3450 2643 1437 850 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2485 3232 5038 5962 8185 10207 2233 Size: 252 747 1806 924 2223 2022 5248 Fragments arranged by size: 5248 2223 2022 1806 924 747 252 BstYI r'GATC_y Cuts at: 1972 2205 2427 3307 3591 4070 5393 6772 9700 Size: 233 222 880 284 479 1323 1379 2928 Cuts at: 9700 10552 10919 11304 11321 12773 12859 12871 13217 Size: 852 367 385 17 1452 86 12 346 Cuts at: 13217 1972 Size: 1977 Fragments arranged by size: 2928 1977 1452 1379 1323 880 852 479 385 367 346 284 233 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3142 3281 5972 3142 Size: 139 2691 10392 Fragments arranged by size: 10392 2691 139 BtgI C'CryG_G Cuts at: 513 1065 3225 4324 7119 8142 10054 513 Size: 552 2160 1099 2795 1023 1912 3681 Fragments arranged by size: 3681 2795 2160 1912 1099 1023 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5796 6678 487 Size: 683 56 4570 882 7031 Fragments arranged by size: 7031 4570 882 683 56 BtsI GCAGTG_nn' Cuts at: 2501 3210 5383 6241 6373 7800 8307 8776 10738 Size: 709 2173 858 132 1427 507 469 1962 Cuts at: 10738 11532 11552 2501 Size: 794 20 4171 Fragments arranged by size: 4171 2173 1962 1427 858 794 709 507 469 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13222 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4078 4113 5398 5433 7976 8011 529 Size: 35 3514 35 1285 35 2543 35 5740 Fragments arranged by size: 5740 3514 2543 1285 35 35 35 35 DraI TTT'AAA Cuts at: 7411 10526 7411 Size: 3115 10107 DraIII CAC_nnn'GTG Cuts at: 3682 9961 3682 Size: 6279 6943 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4178 4833 5477 8712 8753 12229 809 Size: 507 2862 655 644 3235 41 3476 1802 Fragments arranged by size: 3476 3235 2862 1802 655 644 507 41 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2785 2992 Size: 54 1425 50 275 18 285 670 207 Cuts at: 2992 3207 3249 3619 3743 4299 4435 4650 5367 Size: 215 42 370 124 556 136 215 717 Cuts at: 5367 5786 6226 6930 8175 8241 9039 9246 10669 Size: 419 440 704 1245 66 798 207 1423 Cuts at: 10669 11558 13187 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1423 1245 889 798 717 704 670 556 440 419 370 285 275 215 215 207 207 136 124 66 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 4435 10669 1537 Size: 275 2623 6234 4090 Fragments arranged by size: 6234 4090 2623 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 2393 3369 3400 3658 4544 4805 4920 Size: 959 81 976 31 258 886 261 115 Cuts at: 4920 5032 5109 5719 6783 6994 7595 7655 8378 Size: 112 77 610 1064 211 601 60 723 Cuts at: 8378 9536 11152 1353 Size: 1158 1616 3423 Fragments arranged by size: 3423 1616 1158 1064 976 959 886 723 610 601 261 258 211 115 112 81 77 60 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3211 4063 4352 4865 7155 11809 Size: 1064 138 1622 852 289 513 2290 4654 Cuts at: 11809 12183 12329 387 Size: 374 146 1280 Fragments arranged by size: 4654 2290 1622 1280 1064 852 513 374 289 146 138 EcoICRI GAG'CTC Cuts at: 6065 6408 7020 9096 6065 Size: 343 612 2076 10191 Fragments arranged by size: 10191 2076 612 343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2656 2718 2774 3831 4272 4395 5335 Size: 393 1024 62 56 1057 441 123 940 Cuts at: 5335 5586 5829 6009 6051 6161 6417 6723 6960 Size: 251 243 180 42 110 256 306 237 Cuts at: 6960 7267 7679 8316 9434 9566 9603 10088 10229 Size: 307 412 637 1118 132 37 485 141 Cuts at: 10229 10286 10413 10503 11285 11882 12669 12937 1239 Size: 57 127 90 782 597 787 268 1524 Fragments arranged by size: 1524 1118 1057 1024 940 787 782 637 597 485 441 412 393 307 306 268 256 251 243 237 180 141 132 127 123 110 90 62 57 56 42 37 EcoNI CCTnn'n_nnAGG Cuts at: 2225 4533 6140 6451 7986 9582 2225 Size: 2308 1607 311 1535 1596 5865 Fragments arranged by size: 5865 2308 1607 1596 1535 311 EcoO109I rG'GnC_Cy Cuts at: 2336 2692 3274 3583 3790 4415 4855 4965 4966 Size: 356 582 309 207 625 440 110 1 Cuts at: 4966 4976 5197 5984 6459 7071 7588 7702 7828 Size: 10 221 787 475 612 517 114 126 Cuts at: 7828 7860 8617 8618 8667 8755 9618 10140 10329 Size: 32 757 1 49 88 863 522 189 Cuts at: 10329 10481 2336 Size: 152 5077 Fragments arranged by size: 5077 863 787 757 625 612 582 522 517 475 440 356 309 221 207 189 152 126 114 110 88 49 32 10 1 1 EcoRI G'AATT_C Cuts at: 2883 3039 9354 10513 10531 10909 2883 Size: 156 6315 1159 18 378 5196 Fragments arranged by size: 6315 5196 1159 378 156 18 EcoRV GAT'ATC Cuts at: 8896 8896 Size: 13222 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2971 3484 3877 2971 Size: 513 393 12316 Fragments arranged by size: 12316 513 393 FspI TGC'GCA Cuts at: 11728 11728 Size: 13222 HaeII r_GCGC'y Cuts at: 1678 2718 3073 7110 8090 9525 10035 12369 1678 Size: 1040 355 4037 980 1435 510 2334 2531 Fragments arranged by size: 4037 2531 2334 1435 1040 980 510 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2451 2483 2884 2916 2945 Size: 32 135 32 879 32 401 32 29 Cuts at: 2945 2977 3320 3352 5147 5179 5456 5488 5528 Size: 32 343 32 1795 32 277 32 40 Cuts at: 5528 5560 5684 5696 5716 5728 6143 6175 6338 Size: 32 124 12 20 12 415 32 163 Cuts at: 6338 6370 6593 6625 6677 6709 6719 6751 7006 Size: 32 223 32 52 32 10 32 255 Cuts at: 7006 7038 7615 7647 9265 9297 9926 9958 11863 Size: 32 577 32 1618 32 629 32 1905 Cuts at: 11863 11895 11937 11969 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1905 1795 1618 879 629 577 415 401 343 277 255 223 163 135 124 52 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 20 12 12 10 HincII GTy'rAC Cuts at: 669 1657 1938 3288 4357 7693 10027 10566 10812 Size: 988 281 1350 1069 3336 2334 539 246 Cuts at: 10812 669 Size: 3079 Fragments arranged by size: 3336 3079 2334 1350 1069 988 539 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 13222 HpaI GTT'AAC Cuts at: 3288 10812 3288 Size: 7524 5698 KasI G'GCGC_C Cuts at: 8086 10031 8086 Size: 1945 11277 KpnI G_GTAC'C Cuts at: 4542 10547 4542 Size: 6005 7217 MfeI C'AATT_G Cuts at: 10821 10821 Size: 13222 MluI A'CGCG_T Cuts at: 13098 13098 Size: 13222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2437 3582 4130 4195 4677 5321 12336 12520 Size: 1132 1145 548 65 482 644 7015 184 Cuts at: 12520 1305 Size: 2007 Fragments arranged by size: 7015 2007 1145 1132 644 548 482 184 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 3209 3251 4301 5369 6228 Size: 54 1425 343 1377 42 1050 1068 859 Cuts at: 6228 6932 8177 8243 9041 9248 10 Size: 704 1245 66 798 207 3984 Fragments arranged by size: 3984 1425 1377 1245 1068 1050 859 798 704 343 207 66 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3098 3126 3230 4061 5619 8141 Size: 648 1197 735 28 104 831 1558 2522 Cuts at: 8141 9159 10059 10098 10446 11180 11539 11698 518 Size: 1018 900 39 348 734 359 159 2042 Fragments arranged by size: 2522 2042 1558 1197 1018 900 831 735 734 648 359 348 159 104 39 28 MspA1I CmG'CkG Cuts at: 1606 2101 2146 2449 3643 3878 4326 5589 5894 Size: 495 45 303 1194 235 448 1263 305 Cuts at: 5894 6011 7135 7265 7804 7850 8272 8333 8851 Size: 117 1124 130 539 46 422 61 518 Cuts at: 8851 9016 9755 10090 10638 11315 12463 12708 1606 Size: 165 739 335 548 677 1148 245 2120 Fragments arranged by size: 2120 1263 1194 1148 1124 739 677 548 539 518 495 448 422 335 305 303 245 235 165 130 117 61 46 45 NaeI GCC'GGC Cuts at: 4439 9226 4439 Size: 4787 8435 NarI GG'CG_CC Cuts at: 8087 10032 8087 Size: 1945 11277 NcoI C'CATG_G Cuts at: 513 1065 7119 513 Size: 552 6054 6616 Fragments arranged by size: 6616 6054 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 13222 NgoMIV G'CCGG_C Cuts at: 4437 9224 4437 Size: 4787 8435 NheI G'CTAG_C Cuts at: 1052 7109 1052 Size: 6057 7165 NotI GC'GGCC_GC Cuts at: 10669 10669 Size: 13222 NspI r_CATG'y Cuts at: 1132 1229 6225 7996 8140 9475 10580 12125 1132 Size: 97 4996 1771 144 1335 1105 1545 2229 Fragments arranged by size: 4996 2229 1771 1545 1335 1105 144 97 PasI CC'CwG_GG Cuts at: 1348 4144 4160 4961 5720 5817 8365 8557 1348 Size: 2796 16 801 759 97 2548 192 6013 Fragments arranged by size: 6013 2796 2548 801 759 192 97 16 PciI A'CATG_T Cuts at: 12121 12121 Size: 13222 PflMI CCAn_nnn'nTGG Cuts at: 1108 4214 1108 Size: 3106 10116 PfoI T'CCnGG_A Cuts at: 3338 4288 4338 4411 8907 9140 9450 9625 3338 Size: 950 50 73 4496 233 310 175 6935 Fragments arranged by size: 6935 4496 950 310 233 175 73 50 PmeI GTTT'AAAC Cuts at: 10526 10526 Size: 13222 PmlI CAC'GTG Cuts at: 6818 6818 Size: 13222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5302 5334 9389 9421 11291 11323 12804 12836 5302 Size: 32 4055 32 1870 32 1481 32 5688 Fragments arranged by size: 5688 4055 1870 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2336 3274 3583 4415 4976 5984 7588 7828 7860 Size: 938 309 832 561 1008 1604 240 32 Cuts at: 7860 8667 8755 10481 2336 Size: 807 88 1726 5077 Fragments arranged by size: 5077 1726 1604 1008 938 832 807 561 309 240 88 32 PshAI GACnn'nnGTC Cuts at: 1613 7949 1613 Size: 6336 6886 PsiI TTA'TAA Cuts at: 2565 10792 2565 Size: 8227 4995 PspOMI G'GGCC_C Cuts at: 4163 4965 8617 10328 4163 Size: 802 3652 1711 7057 Fragments arranged by size: 7057 3652 1711 802 PspXI vC'TCGA_Gb Cuts at: 6838 6838 Size: 13222 PstI C_TGCA'G Cuts at: 830 2064 2076 2133 3883 4691 5194 6319 9206 Size: 1234 12 57 1750 808 503 1125 2887 Cuts at: 9206 9497 10574 830 Size: 291 1077 3478 Fragments arranged by size: 3478 2887 1750 1234 1125 1077 808 503 291 57 12 PvuI CG_AT'CG Cuts at: 2001 8251 2001 Size: 6250 6972 PvuII CAG'CTG Cuts at: 2146 2449 3643 3878 5589 6011 7265 7850 8272 Size: 303 1194 235 1711 422 1254 585 422 Cuts at: 8272 8333 10090 2146 Size: 61 1757 5278 Fragments arranged by size: 5278 1757 1711 1254 1194 585 422 422 303 235 61 SacI G_AGCT'C Cuts at: 6067 6410 7022 9098 6067 Size: 343 612 2076 10191 Fragments arranged by size: 10191 2076 612 343 SacII CC_GC'GG Cuts at: 4327 4327 Size: 13222 SalI G'TCGA_C Cuts at: 1936 10564 1936 Size: 8628 4594 SanDI GG'GwC_CC Cuts at: 3583 4976 3583 Size: 1393 11829 SapI GCTCTTCn'nnn_ Cuts at: 3400 4805 9536 3400 Size: 1405 4731 7086 Fragments arranged by size: 7086 4731 1405 SbfI CC_TGCA'GG Cuts at: 2076 10574 2076 Size: 8498 4724 ScaI AGT'ACT Cuts at: 4607 11470 13182 4607 Size: 6863 1712 4647 Fragments arranged by size: 6863 4647 1712 SfcI C'TryA_G Cuts at: 826 1045 2060 2072 2129 3879 4687 5190 5763 Size: 219 1015 12 57 1750 808 503 573 Cuts at: 5763 5908 6315 7364 9057 9202 9493 10570 11705 Size: 145 407 1049 1693 145 291 1077 1135 Cuts at: 11705 12386 12577 826 Size: 681 191 1471 Fragments arranged by size: 1750 1693 1471 1135 1077 1049 1015 808 681 573 503 407 291 219 191 145 145 57 12 SfoI GGC'GCC Cuts at: 8088 10033 8088 Size: 1945 11277 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13222 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13222 SmaI CCC'GGG Cuts at: 1872 4677 4974 5880 6252 6432 10549 1872 Size: 2805 297 906 372 180 4117 4545 Fragments arranged by size: 4545 4117 2805 906 372 297 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 2822 3096 3506 3539 3703 4396 Size: 197 926 879 274 410 33 164 693 Cuts at: 4396 5473 6163 6838 7673 8692 9193 9466 11326 Size: 1077 690 675 835 1019 501 273 1860 Cuts at: 11326 12227 12489 820 Size: 901 262 1553 Fragments arranged by size: 1860 1553 1077 1019 926 901 879 835 693 690 675 501 410 274 273 262 197 164 33 SnaBI TAC'GTA Cuts at: 493 11013 493 Size: 10520 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 13222 SphI G_CATG'C Cuts at: 1132 9475 10580 1132 Size: 8343 1105 3774 Fragments arranged by size: 8343 3774 1105 SspI AAT'ATT Cuts at: 5 52 10459 11146 5 Size: 47 10407 687 2081 Fragments arranged by size: 10407 2081 687 47 StuI AGG'CCT Cuts at: 1517 2677 4987 5343 6621 1517 Size: 1160 2310 356 1278 8118 Fragments arranged by size: 8118 2310 1278 1160 356 StyI C'CwwG_G Cuts at: 513 1065 2226 2610 2706 3459 3728 3793 4167 Size: 552 1161 384 96 753 269 65 374 Cuts at: 4167 5031 5202 7119 7432 8651 10332 10661 513 Size: 864 171 1917 313 1219 1681 329 3074 Fragments arranged by size: 3074 1917 1681 1219 1161 864 753 552 384 374 329 313 269 171 96 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2353 2609 2640 4069 5272 5303 Size: 1255 450 323 256 31 1429 1203 31 Cuts at: 5303 6396 6614 6875 9171 9679 11249 11266 11419 Size: 1093 218 261 2296 508 1570 17 153 Cuts at: 11419 11604 12049 325 Size: 185 445 1498 Fragments arranged by size: 2296 1570 1498 1429 1255 1203 1093 508 450 445 323 261 256 218 185 153 31 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2210 3529 Size: 274 80 33 51 1443 159 74 1319 Cuts at: 3529 3771 4605 10237 10350 11468 13180 96 Size: 242 834 5632 113 1118 1712 138 Fragments arranged by size: 5632 1712 1443 1319 1118 834 274 242 159 138 113 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4842 4874 5278 5310 7791 7823 8416 8448 9897 Size: 32 404 32 2481 32 593 32 1449 Cuts at: 9897 9929 13182 13214 4842 Size: 32 3253 32 4850 Fragments arranged by size: 4850 3253 2481 1449 593 404 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 10290 1316 Size: 8974 4248 XbaI T'CTAG_A Cuts at: 2845 6575 10558 2845 Size: 3730 3983 5509 Fragments arranged by size: 5509 3983 3730 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2749 3466 4622 4790 5686 7507 2749 Size: 717 1156 168 896 1821 8464 Fragments arranged by size: 8464 1821 1156 896 717 168 XhoI C'TCGA_G Cuts at: 1943 5473 6838 1943 Size: 3530 1365 8327 Fragments arranged by size: 8327 3530 1365 XmaI C'CCGG_G Cuts at: 1870 4675 4972 5878 6250 6430 10547 1870 Size: 2805 297 906 372 180 4117 4545 Fragments arranged by size: 4545 4117 2805 906 372 297 180 XmnI GAAnn'nnTTC Cuts at: 3963 7617 11351 3963 Size: 3654 3734 5834 Fragments arranged by size: 5834 3734 3654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5044 11030 276 Size: 53 83 186 936 3510 5986 2468 Fragments arranged by size: 5986 3510 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PsrI RsrII SexAI SfiI SrfI SwaI