(Circular) (Six-base) MAPSORT of: pFN21AE0406.seq Check: 426 from: 1 to: 5728 pFN21AE0406 5728 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2396 2396 Size: 5728 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2365 3791 5175 5443 1632 Size: 713 20 1426 1384 268 1917 Fragments arranged by size: 1917 1426 1384 713 268 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 2346 4627 5604 1203 Size: 1143 2281 977 1327 Fragments arranged by size: 2281 1327 1143 977 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AlwNI CAG_nnn'CTG Cuts at: 2481 3471 5043 2481 Size: 990 1572 3166 Fragments arranged by size: 3166 1572 990 ApaI G_GGCC'C Cuts at: 2391 2391 Size: 5728 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 2276 2485 2582 3037 3263 3415 5524 1493 Size: 783 209 97 455 226 152 2109 1697 Fragments arranged by size: 2109 1697 783 455 226 209 152 97 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 3053 1870 Size: 73 1110 4545 Fragments arranged by size: 4545 1110 73 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 3049 4504 618 Size: 325 217 627 609 653 1455 1842 Fragments arranged by size: 1842 1455 653 627 609 325 217 BanII G_rGCy'C Cuts at: 1391 2391 3047 1391 Size: 1000 656 4072 Fragments arranged by size: 4072 1000 656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 2810 928 Size: 971 472 439 3846 Fragments arranged by size: 3846 971 472 439 BbvCI CC'TCA_GC Cuts at: 2401 2401 Size: 5728 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4339 136 Size: 107 122 71 631 605 337 2330 1525 Fragments arranged by size: 2330 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 2391 2454 3789 4945 1790 Size: 601 63 1335 1156 2573 Fragments arranged by size: 2573 1335 1156 601 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 3459 4417 Size: 416 53 83 399 24 711 1331 958 Cuts at: 4417 442 Size: 1753 Fragments arranged by size: 1753 1331 958 711 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2989 2989 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4388 1239 Size: 3149 2579 Bpu10I CC'TnA_GC Cuts at: 2401 2401 Size: 5728 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3853 4718 5016 3853 Size: 865 298 4565 Fragments arranged by size: 4565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2026 4391 882 Size: 617 527 2365 2219 Fragments arranged by size: 2365 2219 617 527 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 2532 5722 2532 Size: 3190 2538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2590 4161 4559 4833 4980 5391 2590 Size: 1571 398 274 147 411 2927 Fragments arranged by size: 2927 1571 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 1178 Size: 30 165 30 709 30 4764 Fragments arranged by size: 4764 709 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 4815 BseYI C'CCAG_C Cuts at: 2255 2418 4931 5427 2255 Size: 163 2513 496 2556 Fragments arranged by size: 2556 2513 496 163 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2265 1163 Size: 573 529 4626 Fragments arranged by size: 4626 573 529 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3789 3874 4945 3789 Size: 85 1071 4572 Fragments arranged by size: 4572 1071 85 BsmI GAATG_Cn' Cuts at: 3239 3332 3239 Size: 93 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2454 3047 3789 3874 4945 1391 Size: 399 601 63 593 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 593 399 85 63 BspEI T'CCGG_A Cuts at: 2590 5391 2590 Size: 2801 2927 BspHI T'CATG_A Cuts at: 2799 3617 5347 2799 Size: 818 1730 3180 Fragments arranged by size: 3180 1730 818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2396 3083 844 Size: 1552 687 3489 Fragments arranged by size: 3489 1552 687 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4223 4397 65 Size: 1183 2975 174 1396 Fragments arranged by size: 2975 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4372 4559 1700 Size: 110 2562 187 2869 Fragments arranged by size: 2869 2562 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5728 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstXI CCAn_nnnn'nTGG Cuts at: 2141 2878 2141 Size: 737 4991 BstYI r'GATC_y Cuts at: 1972 3058 3425 3810 3827 5279 5365 5377 5723 Size: 1086 367 385 17 1452 86 12 346 Cuts at: 5723 1972 Size: 1977 Fragments arranged by size: 1977 1452 1086 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2982 2982 Size: 5728 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4989 Fragments arranged by size: 4989 683 56 BtsI GCAGTG_nn' Cuts at: 2452 2692 2718 3244 4038 4058 2452 Size: 240 26 526 794 20 4122 Fragments arranged by size: 4122 794 526 240 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2667 2702 529 Size: 35 2103 35 3555 Fragments arranged by size: 3555 2103 35 35 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2837 3175 4064 Size: 54 1425 50 275 18 1007 338 889 Cuts at: 4064 5693 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1007 889 338 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3658 1353 Size: 2305 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2365 3791 4388 5175 5443 1239 Size: 393 713 20 1426 597 787 268 1524 Fragments arranged by size: 1524 1426 787 713 597 393 268 20 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2042 Size: 5728 EcoO109I rG'GnC_Cy Cuts at: 2387 2885 2387 Size: 498 5230 EcoRI G'AATT_C Cuts at: 2485 3037 3415 2485 Size: 552 378 4798 Fragments arranged by size: 4798 552 378 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2797 2829 2847 2879 4369 Size: 32 135 32 1225 32 18 32 1490 Cuts at: 4369 4401 4443 4475 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1490 1225 135 42 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3072 3318 669 Size: 988 281 1134 246 3079 Fragments arranged by size: 3079 1134 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2839 10 Size: 54 1425 343 1007 2899 Fragments arranged by size: 2899 1425 1007 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3686 4045 4204 518 Size: 648 2520 359 159 2042 Fragments arranged by size: 2520 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2330 3144 3821 4969 5214 1606 Size: 724 814 677 1148 245 2120 Fragments arranged by size: 2120 1148 814 724 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4237 Fragments arranged by size: 4237 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 2350 3086 4631 1132 Size: 97 1121 736 1545 2229 Fragments arranged by size: 2229 1545 1121 736 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 2346 4627 2346 Size: 2281 3447 PflMI CCAn_nnn'nTGG Cuts at: 1108 2846 1108 Size: 1738 3990 PfoI T'CCnGG_A Cuts at: 2901 2901 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2885 2885 Size: 5728 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 2321 3298 2321 Size: 977 4751 PspOMI G'GGCC_C Cuts at: 2387 2387 Size: 5728 PstI C_TGCA'G Cuts at: 830 2710 3080 830 Size: 1880 370 3478 Fragments arranged by size: 3478 1880 370 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2330 2330 Size: 5728 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SexAI A'CCwGG_T Cuts at: 2313 2313 Size: 5728 SfcI C'TryA_G Cuts at: 826 1045 2238 2373 2706 3076 4211 4892 5083 Size: 219 1193 135 333 370 1135 681 191 Cuts at: 5083 826 Size: 1471 Fragments arranged by size: 1471 1193 1135 681 370 333 219 191 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3832 4733 4995 820 Size: 197 926 1889 901 262 1553 Fragments arranged by size: 1889 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 3025 3652 5 Size: 47 2973 627 2081 Fragments arranged by size: 2973 2081 627 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2004 3167 513 Size: 552 939 1163 3074 Fragments arranged by size: 3074 1163 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3755 3772 3925 4110 4555 325 Size: 1255 2175 17 153 185 445 1498 Fragments arranged by size: 2175 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3974 5686 96 Size: 274 80 33 51 1443 1997 1712 138 Fragments arranged by size: 1997 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5688 5720 5688 Size: 32 5696 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 2962 3064 2962 Size: 102 5626 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2215 2767 2215 Size: 552 5176 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5728 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 3857 3857 Size: 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI