################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I AspEI AvrII BbeI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI FseI HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1152 GACGTC GACGT^C Acc65I 1037 GGTACC G^GTACC AcyI 1152 GACGTC G[GA]^CG[CT]C Alw44I 793 GTGCAC G^TGCAC Aor51HI 2051 AGCGCT AGC^GCT ApaLI 793 GTGCAC G^TGCAC Asp718I 1037 GGTACC G^GTACC AspI 1699 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1529 TGCGCA TGC^GCA BbsI 1861 GAAGAC GAAGAC(2/6) BbvCI 1160 CCTCAGC CCTCAGC(-5/-2) BclI 1626 TGATCA T^GATCA BpuAI 1861 GAAGAC GAAGAC(2/6) BsaBI 2492 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1152 GACGTC G[GA]^CG[CT]C BsmBI 1226 GAGACG CGTCTC(1/5) BsmI 728 GAATGC GAATGC(1/-1) BspHI 1661 TCATGA T^CATGA BstEII 2101 GGTGACC G^GT[ACGT]ACC BstPI 2101 GGTGACC G^GT[ACGT]ACC DrdI 736 GACGGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2051 AGCGCT AGC^GCT EcoO65I 2101 GGTGACC G^GT[ACGT]ACC EcoRI 2245 GAATTC G^AATTC EcoRV 2515 GATATC GAT^ATC EcoT22I 2109 ATGCAT ATGCA^T FbaI 1626 TGATCA T^GATCA FspI 1529 TGCGCA TGC^GCA Hin1I 1152 GACGTC G[GA]^CG[CT]C HincII 1504 GTCAAC GT[CT]^[GA]AC HindII 1504 GTCAAC GT[CT]^[GA]AC HindIII 1469 AAGCTT A^AGCTT KpnI 1037 GGTACC GGTAC^C MamI 2492 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 2109 ATGCAT ATGCA^T PaeR7I 255 CTCGAG C^TCGAG PflFI 1699 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1661 TCATGA T^CATGA SbfI 2283 CCTGCAGG CCTGCA^GG SmaI 27 CCCGGG CCC^GGG SmlI 255 CTCGAG C^T[CT][GA]AG Sse8387I 2283 CCTGCAGG CCTGCA^GG StuI 903 AGGCCT AGG^CCT Tth111I 1699 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 292 CCAAAGGTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 255 CTCGAG C^TCGAG XmaCI 27 CCCGGG C^CCGGG XmaI 27 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 100 GTCTAC GT^[AC][GT]AC 366 GTCTAC AccII 95 CGCG CG^CG 97 CGCG AcsI 1822 GAATTT [GA]^AATT[CT] 2245 GAATTC ApaI 197 GGGCCC GGGCC^C 816 GGGCCC ApoI 1822 GAATTT [GA]^AATT[CT] 2245 GAATTC BamHI 305 GGATCC G^GATCC 2290 GGATCC BanI 1037 GGTACC G^G[CT][GA]CC 1789 GGCACC BanII 197 GGGCCC G[GA]GC[CT]^C 816 GGGCCC BbrPI 790 CACGTG CAC^GTG 1396 CACGTG BcgI 633 GCATGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 670 GCAAACAATTCG BfaI 1595 CTAG C^TAG 2570 CTAG BglI 1165 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2021 GCCCCCCTGGC BglII 537 AGATCT A^GATCT 1116 AGATCT BsaAI 790 CACGTG [CT]AC^GT[GA] 1396 CACGTG BsaI 1559 GAGACC GGTCTC(1/5) 1598 GGTCTC BseRI 719 GAGGAG GAGGAG(10/8) 1556 GAGGAG BspLU11I 2236 ACATGT A^CATGT 2722 ACATGT BspMI 41 ACCTGC ACCTGC(4/8) 1897 GCAGGT BstUI 95 CGCG CG^CG 97 CGCG BstXI 1317 CCATCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1352 CCATGGTGATGG FauI 1082 GCGGG CCCGC(4/6) 1261 GCGGG MaeI 1595 CTAG C^TAG 2570 CTAG MvnI 95 CGCG CG^CG 97 CGCG PflMI 107 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 395 CCAGCATGTGG PmaCI 790 CACGTG CAC^GTG 1396 CACGTG PmlI 790 CACGTG CAC^GTG 1396 CACGTG PshAI 93 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 349 GACTTCAGTC TaqI 256 TCGA T^CGA 455 TCGA TthHB8I 256 TCGA T^CGA 455 TCGA Van91I 107 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 395 CCAGCATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BbvI BciVI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII