(Circular) (Six-base) MAPSORT of: pFN21AB8414.seq Check: 4345 from: 1 to: 7444 pFN21AB8414 7444 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3161 5254 278 Size: 53 83 186 936 1625 2093 2468 Fragments arranged by size: 2468 2093 1625 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 2372 4787 1937 Size: 169 266 2415 4594 Fragments arranged by size: 4594 2415 266 169 Acc65I G'GTAC_C Cuts at: 3042 4765 3042 Size: 1723 5721 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2341 3462 4470 5507 6891 7159 1632 Size: 596 113 1121 1008 1037 1384 268 1917 Fragments arranged by size: 1917 1384 1121 1037 1008 596 268 113 AfeI AGC'GCT Cuts at: 4058 4058 Size: 7444 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7247 AflIII A'CryG_T Cuts at: 1203 3402 4241 4727 6343 7320 1203 Size: 2199 839 486 1616 977 1327 Fragments arranged by size: 2199 1616 1327 977 839 486 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 2421 3405 3800 1166 Size: 1255 984 395 4810 Fragments arranged by size: 4810 1255 984 395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3818 3925 1184 Size: 2634 107 4703 Fragments arranged by size: 4703 2634 107 AlwNI CAG_nnn'CTG Cuts at: 2672 4011 4394 5187 6759 2672 Size: 1339 383 793 1572 3357 Fragments arranged by size: 3357 1572 1339 793 383 ApaI G_GGCC'C Cuts at: 2206 2825 2206 Size: 619 6825 ApaLI G'TGCA_C Cuts at: 2798 5501 6657 2798 Size: 2703 1156 3585 Fragments arranged by size: 3585 2703 1156 ApoI r'AATT_y Cuts at: 1493 3827 4250 4753 4979 5131 7240 1493 Size: 2334 423 503 226 152 2109 1697 Fragments arranged by size: 2334 2109 1697 503 423 226 152 AseI AT'TA_AT Cuts at: 160 1032 5998 160 Size: 872 4966 1606 Fragments arranged by size: 4966 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2069 2260 4769 1870 Size: 73 89 37 191 2509 4545 Fragments arranged by size: 4545 2509 191 89 73 37 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3026 3059 3026 Size: 33 7411 BamHI G'GATC_C Cuts at: 2310 4295 4774 5141 2310 Size: 1985 479 367 4613 Fragments arranged by size: 4613 1985 479 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3042 3794 4765 6220 618 Size: 325 217 627 1255 752 971 1455 1842 Fragments arranged by size: 1842 1455 1255 971 752 627 325 217 BanII G_rGCy'C Cuts at: 1391 2206 2825 4763 1391 Size: 815 619 1938 4072 Fragments arranged by size: 4072 1938 815 619 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3873 928 Size: 971 1974 4499 Fragments arranged by size: 4499 1974 971 BbvCI CC'TCA_GC Cuts at: 3166 3166 Size: 7444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2627 2661 2664 2698 5635 5669 1903 Size: 34 690 34 3 34 2937 34 3678 Fragments arranged by size: 3678 2937 690 34 34 34 34 3 BciVI GTATCCnnnnn_n' Cuts at: 4127 4189 4490 5336 6546 4127 Size: 62 301 846 1210 5025 Fragments arranged by size: 5025 1210 846 301 62 BclI T'GATC_A Cuts at: 1548 3631 1548 Size: 2083 5361 BfrBI ATG'CAT Cuts at: 4116 4116 Size: 7444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3176 4032 6055 Size: 107 122 71 631 605 1504 856 2023 Cuts at: 6055 136 Size: 1525 Fragments arranged by size: 2023 1525 1504 856 631 605 122 107 71 BglII A'GATC_T Cuts at: 2542 3121 7439 2542 Size: 579 4318 2547 Fragments arranged by size: 4318 2547 579 BlpI GC'TnA_GC Cuts at: 4861 4861 Size: 7444 Bme1580I G_kGCm'C Cuts at: 1790 2206 2368 2802 2825 4075 5505 6661 1790 Size: 416 162 434 23 1250 1430 1156 2573 Fragments arranged by size: 2573 1430 1250 1156 434 416 162 23 BmgBI CAC'GTC Cuts at: 1318 2581 3526 4536 1318 Size: 1263 945 1010 4226 Fragments arranged by size: 4226 1263 1010 945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 2362 3162 Size: 416 53 83 399 24 711 234 800 Cuts at: 3162 3667 3715 5175 6133 442 Size: 505 48 1460 958 1753 Fragments arranged by size: 1753 1460 958 800 711 505 416 399 234 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2676 3247 3539 3741 4003 6104 1239 Size: 1437 571 292 202 262 2101 2579 Fragments arranged by size: 2579 2101 1437 571 292 262 202 Bpu10I CC'TnA_GC Cuts at: 3166 3740 3166 Size: 574 6870 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5569 6434 6732 5569 Size: 865 298 6281 Fragments arranged by size: 6281 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3558 3609 6107 882 Size: 617 2059 51 2498 2219 Fragments arranged by size: 2498 2219 2059 617 51 BsaAI yAC'GTr Cuts at: 493 1204 2797 3403 5235 493 Size: 711 1593 606 1832 2702 Fragments arranged by size: 2702 1832 1593 711 606 BsaBI GATnn'nnATC Cuts at: 4501 7438 4501 Size: 2937 4507 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3158 5251 5633 6268 Size: 53 83 186 936 1625 2093 382 635 Cuts at: 6268 275 Size: 1451 Fragments arranged by size: 2093 1625 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5877 6275 6549 6696 7107 5877 Size: 398 274 147 411 6214 Fragments arranged by size: 6214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2951 2981 2987 3017 3782 Size: 30 165 30 1548 30 6 30 765 Cuts at: 3782 3812 4090 4120 1178 Size: 30 278 30 4502 Fragments arranged by size: 4502 1548 765 278 165 30 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2739 3576 1186 Size: 1553 837 5054 Fragments arranged by size: 5054 1553 837 BseYI C'CCAG_C Cuts at: 2010 2399 2790 2817 3681 4382 6647 7143 2010 Size: 389 391 27 864 701 2265 496 2311 Fragments arranged by size: 2311 2265 864 701 496 391 389 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2674 3474 3824 3943 4396 4469 1163 Size: 573 938 800 350 119 453 73 4138 Fragments arranged by size: 4138 938 800 573 453 350 119 73 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 5655 6683 1540 Size: 275 186 2893 761 1028 2301 Fragments arranged by size: 2893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2802 3419 3525 4307 5505 5590 6661 2802 Size: 617 106 782 1198 85 1071 3585 Fragments arranged by size: 3585 1198 1071 782 617 106 85 BsmI GAATG_Cn' Cuts at: 2739 4955 5048 2739 Size: 2216 93 5135 Fragments arranged by size: 5135 2216 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3225 3225 Size: 7444 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2206 2368 2802 2825 3419 3525 4075 Size: 399 416 162 434 23 594 106 550 Cuts at: 4075 4307 4763 5505 5590 6661 1391 Size: 232 456 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 550 456 434 416 399 232 162 106 85 23 BspEI T'CCGG_A Cuts at: 7107 7107 Size: 7444 BspHI T'CATG_A Cuts at: 3666 5333 7063 3666 Size: 1667 1730 4047 Fragments arranged by size: 4047 1730 1667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 3893 4799 844 Size: 1211 1838 906 3489 Fragments arranged by size: 3489 1838 1211 906 BsrBI CCG'CTC Cuts at: 1394 1823 5331 1394 Size: 429 3508 3507 Fragments arranged by size: 3508 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3189 3676 3970 4546 5939 6113 65 Size: 1183 1941 487 294 576 1393 174 1396 Fragments arranged by size: 1941 1396 1393 1183 576 487 294 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6088 6275 1700 Size: 110 4278 187 2869 Fragments arranged by size: 4278 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4262 4562 4730 96 Size: 4166 300 168 2810 Fragments arranged by size: 4166 2810 300 168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 2672 4122 4410 794 Size: 1878 1450 288 3828 Fragments arranged by size: 3828 1878 1450 288 BstBI TT'CG_AA Cuts at: 6248 6248 Size: 7444 BstEII G'GTnAC_C Cuts at: 1184 4106 1184 Size: 2922 4522 BstXI CCAn_nnnn'nTGG Cuts at: 3329 3364 3329 Size: 35 7409 BstYI r'GATC_y Cuts at: 1972 2310 2542 3121 4008 4295 4774 5141 5526 Size: 338 232 579 887 287 479 367 385 Cuts at: 5526 5543 6995 7081 7093 7439 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 887 579 479 385 367 346 338 287 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3174 3357 3639 4102 513 Size: 552 939 1170 183 282 463 3855 Fragments arranged by size: 3855 1170 939 552 463 282 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 2416 2428 2686 2962 3015 3462 3931 3958 4171 Size: 12 258 276 53 447 469 27 213 Cuts at: 4171 4231 4960 5754 5774 2416 Size: 60 729 794 20 4086 Fragments arranged by size: 4086 794 729 469 447 276 258 213 60 53 27 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 4748 4748 Size: 7444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2747 6451 809 Size: 507 1431 3704 1802 Fragments arranged by size: 3704 1802 1431 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3069 3570 3615 Size: 54 1425 50 275 18 1239 501 45 Cuts at: 3615 4033 4339 4891 5780 7409 8 Size: 418 306 552 889 1629 43 Fragments arranged by size: 1629 1425 1239 889 552 501 418 306 275 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4891 1537 Size: 275 3079 4090 Fragments arranged by size: 4090 3079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2468 3045 4489 4632 5374 1353 Size: 1115 577 1444 143 742 3423 Fragments arranged by size: 3423 1444 1115 742 577 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2917 3431 4664 6031 6405 6551 Size: 1064 138 1328 514 1233 1367 374 146 Cuts at: 6551 387 Size: 1280 Fragments arranged by size: 1367 1328 1280 1233 1064 514 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2341 2676 3247 3462 3539 3741 Size: 393 596 113 335 571 215 77 202 Cuts at: 3741 4003 4470 5507 6104 6891 7159 1239 Size: 262 467 1037 597 787 268 1524 Fragments arranged by size: 1524 1037 787 597 596 571 467 393 335 268 262 215 202 113 77 EcoO109I rG'GnC_Cy Cuts at: 2202 2203 2271 2821 3709 3905 2202 Size: 1 68 550 888 196 5741 Fragments arranged by size: 5741 888 550 196 68 1 EcoRI G'AATT_C Cuts at: 4250 4753 5131 4250 Size: 503 378 6563 Fragments arranged by size: 6563 503 378 EcoRV GAT'ATC Cuts at: 4522 4522 Size: 7444 FspI TGC'GCA Cuts at: 3536 5950 3536 Size: 2414 5030 FspAI rTGC'GCAy Cuts at: 3536 3536 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 2066 2814 4060 6591 1678 Size: 388 748 1246 2531 2531 Fragments arranged by size: 2531 2531 1246 748 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 2951 2983 3044 Size: 32 135 32 949 32 398 32 61 Cuts at: 3044 3076 3716 3748 6085 6117 6159 6191 1373 Size: 32 640 32 2337 32 42 32 2626 Fragments arranged by size: 2626 2337 949 640 398 135 61 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3511 4788 5034 669 Size: 988 281 1573 1277 246 3079 Fragments arranged by size: 3079 1573 1277 988 281 246 HindIII A'AGCT_T Cuts at: 748 3474 748 Size: 2726 4718 HpaI GTT'AAC Cuts at: 5034 5034 Size: 7444 KpnI G_GTAC'C Cuts at: 3046 4769 3046 Size: 1723 5721 MfeI C'AATT_G Cuts at: 5043 5043 Size: 7444 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2075 2338 2677 6558 6742 1305 Size: 770 263 339 3881 184 2007 Fragments arranged by size: 3881 2007 770 339 263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3071 3572 3617 4035 10 Size: 54 1425 343 1239 501 45 418 3419 Fragments arranged by size: 3419 1425 1239 501 418 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2421 2756 3327 3362 3405 3800 Size: 648 1122 133 335 571 35 43 395 Cuts at: 3800 3963 5402 5761 5920 518 Size: 163 1439 359 159 2042 Fragments arranged by size: 2042 1439 1122 648 571 395 359 335 163 159 133 43 35 MspA1I CmG'CkG Cuts at: 1606 2022 2672 2817 3087 4023 4161 4860 5537 Size: 416 650 145 270 936 138 699 677 Cuts at: 5537 6685 6930 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 936 699 677 650 416 270 245 145 138 NcoI C'CATG_G Cuts at: 513 1065 2004 3174 3357 3639 4102 513 Size: 552 939 1170 183 282 463 3855 Fragments arranged by size: 3855 1170 939 552 463 282 183 NdeI CA'TA_TG Cuts at: 387 387 Size: 7444 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NsiI A_TGCA'T Cuts at: 4118 4118 Size: 7444 NspI r_CATG'y Cuts at: 1132 1229 2407 3245 4120 4245 4731 4802 6347 Size: 97 1178 838 875 125 486 71 1545 Cuts at: 6347 1132 Size: 2229 Fragments arranged by size: 2229 1545 1178 875 838 486 125 97 71 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7444 PciI A'CATG_T Cuts at: 4241 4727 6343 4241 Size: 486 1616 5342 Fragments arranged by size: 5342 1616 486 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2118 2406 1108 Size: 902 108 288 6146 Fragments arranged by size: 6146 902 288 108 PfoI T'CCnGG_A Cuts at: 2145 2511 2145 Size: 366 7078 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PmlI CAC'GTG Cuts at: 2797 3403 2797 Size: 606 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3589 3621 5513 5545 7026 7058 3589 Size: 32 1892 32 1481 32 3975 Fragments arranged by size: 3975 1892 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2271 3709 3905 2271 Size: 1438 196 5810 Fragments arranged by size: 5810 1438 196 PshAI GACnn'nnGTC Cuts at: 1613 2102 2358 1613 Size: 489 256 6699 Fragments arranged by size: 6699 489 256 PsiI TTA'TAA Cuts at: 5014 5014 Size: 7444 PspOMI G'GGCC_C Cuts at: 2202 2821 2202 Size: 619 6825 PstI C_TGCA'G Cuts at: 830 2423 3022 3184 4064 4293 4796 830 Size: 1593 599 162 880 229 503 3478 Fragments arranged by size: 3478 1593 880 599 503 229 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 2817 4023 4161 2817 Size: 1206 138 6100 Fragments arranged by size: 6100 1206 138 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SanDI GG'GwC_CC Cuts at: 2271 2271 Size: 7444 SbfI CC_TGCA'GG Cuts at: 4293 4796 4293 Size: 503 6941 ScaI AGT'ACT Cuts at: 5692 7404 5692 Size: 1712 5732 SfcI C'TryA_G Cuts at: 826 1045 2419 3018 3079 3117 3180 3853 4060 Size: 219 1374 599 61 38 63 673 207 Cuts at: 4060 4289 4456 4792 5927 6608 6799 826 Size: 229 167 336 1135 681 191 1471 Fragments arranged by size: 1471 1374 1135 681 673 599 336 229 219 207 191 167 63 61 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 2034 4771 1872 Size: 162 2737 4545 Fragments arranged by size: 4545 2737 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 2260 5548 6449 6711 820 Size: 197 926 317 3288 901 262 1553 Fragments arranged by size: 3288 1553 926 901 317 262 197 SnaBI TAC'GTA Cuts at: 493 5235 493 Size: 4742 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 4802 1132 Size: 3670 3774 SspI AAT'ATT Cuts at: 5 52 5368 5 Size: 47 5316 2081 Fragments arranged by size: 5316 2081 47 StuI AGG'CCT Cuts at: 1517 2910 1517 Size: 1393 6051 StyI C'CwwG_G Cuts at: 513 1065 2004 3038 3174 3357 3639 4102 4883 Size: 552 939 1034 136 183 282 463 781 Cuts at: 4883 513 Size: 3074 Fragments arranged by size: 3074 1034 939 781 552 463 282 183 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3438 3563 4389 5471 5488 5641 5826 Size: 1255 1858 125 826 1082 17 153 185 Cuts at: 5826 6271 325 Size: 445 1498 Fragments arranged by size: 1858 1498 1255 1082 826 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4244 4262 4562 Size: 274 80 33 51 1443 2267 18 300 Cuts at: 4562 4730 5690 7402 96 Size: 168 960 1712 138 Fragments arranged by size: 2267 1712 1443 960 300 274 168 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2635 2667 3247 3279 4090 4122 4210 Size: 32 567 32 580 32 811 32 88 Cuts at: 4210 4242 7404 7436 2036 Size: 32 3162 32 2044 Fragments arranged by size: 3162 2044 811 580 567 88 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3707 1316 Size: 2391 5053 XbaI T'CTAG_A Cuts at: 4780 4780 Size: 7444 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2304 2304 Size: 7444 XhoI C'TCGA_G Cuts at: 1943 2260 1943 Size: 317 7127 XmaI C'CCGG_G Cuts at: 1870 2032 4769 1870 Size: 162 2737 4545 Fragments arranged by size: 4545 2737 162 XmnI GAAnn'nnTTC Cuts at: 5573 5573 Size: 7444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3159 5252 276 Size: 53 83 186 936 1625 2093 2468 Fragments arranged by size: 2468 2093 1625 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI