(Circular) (Six-base) MAPSORT of: pFN21AB7723.seq Check: 7751 from: 1 to: 5995 pFN21AB7723 5995 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2702 2702 Size: 5995 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 3294 4122 4495 1563 Size: 1731 828 373 3063 Fragments arranged by size: 3063 1731 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 2740 3225 4058 5442 5710 1632 Size: 1070 38 485 833 1384 268 1917 Fragments arranged by size: 1917 1384 1070 833 485 268 38 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 3066 4894 5871 1203 Size: 1863 1828 977 1327 Fragments arranged by size: 1863 1828 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 2206 2423 2432 2441 3032 3059 3738 5310 2206 Size: 217 9 9 591 27 679 1572 2891 Fragments arranged by size: 2891 1572 679 591 217 27 9 9 ApaI G_GGCC'C Cuts at: 2087 2394 2087 Size: 307 5688 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 3304 3530 3682 5791 1493 Size: 1811 226 152 2109 1697 Fragments arranged by size: 2109 1811 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2393 3167 3320 1870 Size: 73 194 256 774 153 4545 Fragments arranged by size: 4545 774 256 194 153 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4771 618 Size: 325 217 627 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2318 2394 3090 3314 1391 Size: 696 231 76 696 224 4072 Fragments arranged by size: 4072 696 696 231 224 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BbvCI CC'TCA_GC Cuts at: 2303 2585 2303 Size: 282 5713 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 4606 136 Size: 107 122 71 631 605 380 2554 1525 Fragments arranged by size: 2554 1525 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 2410 3412 2410 Size: 1002 4993 Bme1580I G_kGCm'C Cuts at: 1790 2087 2394 4056 5212 1790 Size: 297 307 1662 1156 2573 Fragments arranged by size: 2573 1662 1156 307 297 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 3726 4684 Size: 416 53 83 399 24 613 1696 958 Cuts at: 4684 442 Size: 1753 Fragments arranged by size: 1753 1696 958 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2019 2552 3081 4655 1239 Size: 780 533 529 1574 2579 Fragments arranged by size: 2579 1574 780 533 529 Bpu10I CC'TnA_GC Cuts at: 2303 2585 2303 Size: 282 5713 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4120 4985 5283 4120 Size: 865 298 4832 Fragments arranged by size: 4832 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2103 2881 4658 882 Size: 617 604 778 1777 2219 Fragments arranged by size: 2219 1777 778 617 604 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3802 4184 4819 275 Size: 53 83 186 936 2269 382 635 1451 Fragments arranged by size: 2269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4428 4826 5100 5247 5658 4428 Size: 398 274 147 411 4765 Fragments arranged by size: 4765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5770 Fragments arranged by size: 5770 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2342 2840 2852 2951 1186 Size: 1153 3 498 12 99 4230 Fragments arranged by size: 4230 1153 498 99 12 3 BseYI C'CCAG_C Cuts at: 2387 5198 5694 2387 Size: 2811 496 2688 Fragments arranged by size: 2811 2688 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2318 2693 4056 4141 5212 2318 Size: 375 1363 85 1071 3101 Fragments arranged by size: 3101 1363 1071 375 85 BsmI GAATG_Cn' Cuts at: 2307 3506 3599 2307 Size: 1199 93 4703 Fragments arranged by size: 4703 1199 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2318 2394 2693 3090 3314 4056 Size: 399 297 231 76 299 397 224 742 Cuts at: 4056 4141 5212 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 299 297 231 224 85 76 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 2574 3884 5614 2574 Size: 1310 1730 2955 Fragments arranged by size: 2955 1730 1310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2589 2702 3350 844 Size: 1745 113 648 3489 Fragments arranged by size: 3489 1745 648 113 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4490 4664 65 Size: 1183 3242 174 1396 Fragments arranged by size: 3242 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2332 4639 4826 1700 Size: 110 522 2307 187 2869 Fragments arranged by size: 2869 2307 522 187 110 BsrGI T'GTAC_A Cuts at: 96 3183 96 Size: 3087 2908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 2423 2432 794 Size: 1629 9 4357 Fragments arranged by size: 4357 1629 9 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2626 2611 Size: 15 5980 BstYI r'GATC_y Cuts at: 1972 2505 3056 3325 3692 4077 4094 5546 5632 Size: 533 551 269 367 385 17 1452 86 Cuts at: 5632 5644 5990 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 551 533 385 367 346 269 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2610 3191 513 Size: 552 939 606 581 3317 Fragments arranged by size: 3317 939 606 581 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2836 2871 529 Size: 35 2272 35 3653 Fragments arranged by size: 3653 2272 35 35 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2968 5002 809 Size: 507 1652 2034 1802 Fragments arranged by size: 2034 1802 1652 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3442 4331 5960 Size: 54 1425 50 275 18 1612 889 1629 Cuts at: 5960 8 Size: 43 Fragments arranged by size: 1629 1612 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3244 3925 1353 Size: 1891 681 3423 Fragments arranged by size: 3423 1891 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2121 4582 4956 5102 387 Size: 1064 138 532 2461 374 146 1280 Fragments arranged by size: 2461 1280 1064 532 374 146 138 EcoICRI GAG'CTC Cuts at: 2316 2316 Size: 5995 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2019 2552 2702 2740 3081 3225 4058 Size: 393 387 533 150 38 341 144 833 Cuts at: 4058 4655 5442 5710 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 833 787 597 533 393 387 341 268 150 144 38 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2210 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 2053 2083 2084 2134 2776 2053 Size: 30 1 50 642 5272 Fragments arranged by size: 5272 642 50 30 1 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2667 2749 5142 1678 Size: 989 82 2393 2531 Fragments arranged by size: 2531 2393 989 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373 Size: 32 135 32 3064 32 42 32 2626 Fragments arranged by size: 3064 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2904 3154 3315 5109 5293 1305 Size: 1599 250 161 1794 184 2007 Fragments arranged by size: 2007 1794 1599 250 184 161 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2672 2864 3953 4312 4471 518 Size: 648 1506 192 1089 359 159 2042 Fragments arranged by size: 2042 1506 1089 648 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2347 2423 2432 2680 3216 3411 4088 5236 Size: 741 76 9 248 536 195 677 1148 Cuts at: 5236 5481 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 741 677 536 248 245 195 76 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2368 2553 3070 3353 4898 1132 Size: 97 1139 185 517 283 1545 2229 Fragments arranged by size: 2229 1545 1139 517 283 185 97 PasI CC'CwG_GG Cuts at: 1348 2980 1348 Size: 1632 4363 PciI A'CATG_T Cuts at: 3066 4894 3066 Size: 1828 4167 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 1108 Size: 1098 4897 PfoI T'CCnGG_A Cuts at: 2727 2727 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2776 2134 Size: 642 5353 PshAI GACnn'nnGTC Cuts at: 1613 2570 1613 Size: 957 5038 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 2083 2390 2083 Size: 307 5688 PstI C_TGCA'G Cuts at: 830 2178 2428 2437 3028 3347 830 Size: 1348 250 9 591 319 3478 Fragments arranged by size: 3478 1348 591 319 250 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2347 2423 2680 3216 2347 Size: 76 257 536 5126 Fragments arranged by size: 5126 536 257 76 SacI G_AGCT'C Cuts at: 2318 2318 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SanDI GG'GwC_CC Cuts at: 2134 2134 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2174 2424 2433 3024 3343 4478 5159 Size: 219 1129 250 9 591 319 1135 681 Cuts at: 5159 5350 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1129 681 591 319 250 219 191 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2052 2052 Size: 5995 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 2139 3322 1872 Size: 267 1183 4545 Fragments arranged by size: 4545 1183 267 SmlI C'TyrA_G Cuts at: 820 1017 1943 4099 5000 5262 820 Size: 197 926 2156 901 262 1553 Fragments arranged by size: 2156 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5995 StyI C'CwwG_G Cuts at: 513 1065 2004 2832 3434 513 Size: 552 939 828 602 3074 Fragments arranged by size: 3074 939 828 602 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2993 4022 4039 4192 4377 4822 325 Size: 1255 1413 1029 17 153 185 445 1498 Fragments arranged by size: 1498 1413 1255 1029 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3183 4241 5953 Size: 274 80 33 51 1443 1206 1058 1712 Cuts at: 5953 96 Size: 138 Fragments arranged by size: 1712 1443 1206 1058 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 2137 3320 1870 Size: 267 1183 4545 Fragments arranged by size: 4545 1183 267 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI XcmI