(Circular) (Six-base) MAPSORT of: pFN21AE0513.seq Check: 7917 from: 1 to: 5698 pFN21AE0513 5698 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2595 3508 278 Size: 53 83 186 936 1059 913 2468 Fragments arranged by size: 2468 1059 936 913 186 83 53 AccI GT'mk_AC Cuts at: 1937 2417 3041 1937 Size: 480 624 4594 Fragments arranged by size: 4594 624 480 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 2481 3825 4198 1563 Size: 918 1344 373 3063 Fragments arranged by size: 3063 1344 918 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 2870 2907 3761 5145 5413 1632 Size: 914 324 37 854 1384 268 1917 Fragments arranged by size: 1917 1384 914 854 324 268 37 AfeI AGC'GCT Cuts at: 2450 2450 Size: 5698 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 2217 4597 5574 1203 Size: 1014 2380 977 1327 Fragments arranged by size: 2380 1327 1014 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 2413 4427 2413 Size: 2014 3684 AleI CACnn'nnGTG Cuts at: 1166 2327 1166 Size: 1161 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2204 2236 2000 Size: 32 172 32 5462 Fragments arranged by size: 5462 172 32 32 AlwNI CAG_nnn'CTG Cuts at: 2257 2674 2704 3441 5013 2257 Size: 417 30 737 1572 2942 Fragments arranged by size: 2942 1572 737 417 30 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 2129 2783 3007 3233 3385 5494 1493 Size: 636 654 224 226 152 2109 1697 Fragments arranged by size: 2109 1697 654 636 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2183 3023 1870 Size: 73 240 840 4545 Fragments arranged by size: 4545 840 240 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3019 4474 618 Size: 325 217 627 1232 1455 1842 Fragments arranged by size: 1842 1455 1232 627 325 217 BanII G_rGCy'C Cuts at: 1391 2058 2076 2232 2992 3017 1391 Size: 667 18 156 760 25 4072 Fragments arranged by size: 4072 760 667 156 25 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 2867 3115 2867 Size: 248 5450 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2707 3429 4387 Size: 416 53 83 399 24 1290 722 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1290 958 722 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2354 2381 2861 4358 1239 Size: 989 126 27 480 1497 2579 Fragments arranged by size: 2579 1497 989 480 126 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 3823 4688 4986 2252 Size: 1571 865 298 2964 Fragments arranged by size: 2964 1571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 2978 3505 3887 Size: 53 83 186 936 1059 386 527 382 Cuts at: 3887 4522 275 Size: 635 1451 Fragments arranged by size: 1451 1059 936 635 527 386 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2346 2620 4131 4529 4803 4950 5361 2346 Size: 274 1511 398 274 147 411 2683 Fragments arranged by size: 2683 1511 411 398 274 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2006 2030 2036 2204 Size: 30 165 30 597 6 24 6 168 Cuts at: 2204 2234 1178 Size: 30 4642 Fragments arranged by size: 4642 597 168 165 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2084 2225 2378 1186 Size: 895 3 141 153 4506 Fragments arranged by size: 4506 895 153 141 3 BseYI C'CCAG_C Cuts at: 2059 4901 5397 2059 Size: 2842 496 2360 Fragments arranged by size: 2842 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 2717 2756 2804 1163 Size: 573 672 309 39 48 4057 Fragments arranged by size: 4057 672 573 309 48 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2190 2513 2918 2931 3759 3844 4915 2076 Size: 114 323 405 13 828 85 1071 2859 Fragments arranged by size: 2859 1071 828 405 323 114 85 13 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2076 2190 2232 2513 2918 2931 Size: 399 268 18 114 42 281 405 13 Cuts at: 2931 2992 3017 3759 3844 4915 1391 Size: 61 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 281 268 114 85 61 42 25 18 13 BspEI T'CCGG_A Cuts at: 2620 5361 2620 Size: 2741 2957 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 2109 3053 844 Size: 1193 72 944 3489 Fragments arranged by size: 3489 1193 944 72 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2050 Size: 5698 BstYI r'GATC_y Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693 Size: 1056 367 385 17 1452 86 12 346 Cuts at: 5693 1972 Size: 1977 Fragments arranged by size: 1977 1452 1056 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2983 513 Size: 552 939 979 3228 Fragments arranged by size: 3228 979 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2272 487 Size: 683 56 1046 3913 Fragments arranged by size: 3913 1046 683 56 BtsI GCAGTG_nn' Cuts at: 2643 3214 4008 4028 2643 Size: 571 794 20 4313 Fragments arranged by size: 4313 794 571 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DraIII CAC_nnn'GTG Cuts at: 2194 2194 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 3145 4034 Size: 54 1425 50 275 18 612 703 889 Cuts at: 4034 5663 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 703 612 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2181 3628 1353 Size: 828 1447 3423 Fragments arranged by size: 3423 1447 828 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2074 2074 Size: 5698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2354 2381 2546 2861 2870 2907 Size: 393 596 126 27 165 315 9 37 Cuts at: 2907 3761 4358 5145 5413 1239 Size: 854 597 787 268 1524 Fragments arranged by size: 1524 854 787 597 596 393 315 268 165 126 37 27 9 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2210 Size: 5698 EcoO109I rG'GnC_Cy Cuts at: 2122 2407 2860 2122 Size: 285 453 4960 Fragments arranged by size: 4960 453 285 EcoRI G'AATT_C Cuts at: 2783 3007 3385 2783 Size: 224 378 5096 Fragments arranged by size: 5096 378 224 EcoRV GAT'ATC Cuts at: 2893 2893 Size: 5698 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2611 2611 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2267 2452 2840 4845 1678 Size: 589 185 388 2005 2531 Fragments arranged by size: 2531 2005 589 388 185 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 4339 4371 4413 Size: 32 135 32 827 32 1908 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 1908 827 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2665 3042 3288 669 Size: 988 281 727 377 246 3079 Fragments arranged by size: 3079 988 727 377 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 4996 1305 Size: 3507 184 2007 Fragments arranged by size: 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 10 Size: 54 1425 343 612 3264 Fragments arranged by size: 3264 1425 612 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 3656 4015 4174 518 Size: 648 1161 1329 359 159 2042 Fragments arranged by size: 2042 1329 1161 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2359 2871 3114 3791 4939 5184 1606 Size: 457 296 512 243 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 512 457 296 245 243 NcoI C'CATG_G Cuts at: 513 1065 2004 2983 513 Size: 552 939 979 3228 Fragments arranged by size: 3228 979 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2221 3056 4601 1132 Size: 97 992 835 1545 2229 Fragments arranged by size: 2229 1545 992 835 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 2217 4597 2217 Size: 2380 3318 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 2674 1108 Size: 1149 417 4132 Fragments arranged by size: 4132 1149 417 PfoI T'CCnGG_A Cuts at: 2114 2114 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2000 2032 2204 2236 3767 3799 5280 5312 2000 Size: 32 172 32 1531 32 1481 32 2386 Fragments arranged by size: 2386 1531 1481 172 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2407 2122 Size: 285 5413 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2037 2145 2358 2812 3050 830 Size: 1207 108 213 454 238 3478 Fragments arranged by size: 3478 1207 454 238 213 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2359 2871 2359 Size: 512 5186 SacI G_AGCT'C Cuts at: 2076 2076 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2033 2141 2354 2808 3046 4181 4862 Size: 219 988 108 213 454 238 1135 681 Cuts at: 4862 5053 826 Size: 191 1471 Fragments arranged by size: 1471 1135 988 681 454 238 219 213 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 3802 4703 4965 820 Size: 197 926 324 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 324 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 2983 3137 513 Size: 552 939 39 940 154 3074 Fragments arranged by size: 3074 940 939 552 154 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2122 2726 3725 3742 3895 4080 4525 Size: 1255 542 604 999 17 153 185 445 Cuts at: 4525 325 Size: 1498 Fragments arranged by size: 1498 1255 999 604 542 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2485 2720 2383 Size: 102 235 5361 Fragments arranged by size: 5361 235 102 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2593 3506 276 Size: 53 83 186 936 1059 913 2468 Fragments arranged by size: 2468 1059 936 913 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI