(Circular) (Six-base) MAPSORT of: pFN21AB8651.seq Check: 2438 from: 1 to: 6163 pFN21AB8651 6163 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2677 2677 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3506 1937 Size: 1569 4594 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 4226 5610 5878 1632 Size: 1028 1566 1384 268 1917 Fragments arranged by size: 1917 1566 1384 1028 268 AflII C'TTAA_G Cuts at: 820 1017 2609 820 Size: 197 1592 4374 Fragments arranged by size: 4374 1592 197 AflIII A'CryG_T Cuts at: 1203 5062 6039 1203 Size: 3859 977 1327 Fragments arranged by size: 3859 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 3208 4892 3208 Size: 1684 4479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 2058 3013 3906 5478 2058 Size: 955 893 1572 2743 Fragments arranged by size: 2743 1572 955 893 ApaI G_GGCC'C Cuts at: 2194 2226 3304 2194 Size: 32 1078 5053 Fragments arranged by size: 5053 1078 32 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2297 3046 3277 3488 1870 Size: 73 354 749 231 211 4545 Fragments arranged by size: 4545 749 354 231 211 73 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3484 4939 618 Size: 325 217 627 1697 1455 1842 Fragments arranged by size: 1842 1697 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2194 2226 3304 3482 1391 Size: 803 32 1078 178 4072 Fragments arranged by size: 4072 1078 803 178 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3179 928 Size: 971 1280 3912 Fragments arranged by size: 3912 1280 971 BbvCI CC'TCA_GC Cuts at: 2406 2939 2406 Size: 533 5630 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 1548 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 4774 136 Size: 107 122 71 631 605 583 2519 1525 Fragments arranged by size: 2519 1525 631 605 583 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3338 3580 3338 Size: 242 5921 Bme1580I G_kGCm'C Cuts at: 1790 2194 2226 2660 3304 4224 5380 1790 Size: 404 32 434 644 920 1156 2573 Fragments arranged by size: 2573 1156 920 644 434 404 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2221 2659 3055 Size: 416 53 83 399 24 804 438 396 Cuts at: 3055 3894 4852 442 Size: 839 958 1753 Fragments arranged by size: 1753 958 839 804 438 416 399 396 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 2771 2834 4823 1239 Size: 982 550 63 1989 2579 Fragments arranged by size: 2579 1989 982 550 63 Bpu10I CC'TnA_GC Cuts at: 2406 2939 2406 Size: 533 5630 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2549 4288 5153 5451 2549 Size: 1739 865 298 3261 Fragments arranged by size: 3261 1739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2387 4826 882 Size: 617 888 2439 2219 Fragments arranged by size: 2439 2219 888 617 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2973 3270 3970 4352 Size: 53 83 186 936 1440 297 700 382 Cuts at: 4352 4987 275 Size: 635 1451 Fragments arranged by size: 1451 1440 936 700 635 382 297 186 83 53 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2025 2055 2675 2705 3209 Size: 30 165 30 622 30 620 30 504 Cuts at: 3209 3239 1178 Size: 30 4102 Fragments arranged by size: 4102 622 620 504 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2729 2924 3217 1186 Size: 1327 216 195 293 4132 Fragments arranged by size: 4132 1327 293 216 195 BseYI C'CCAG_C Cuts at: 3026 3350 5366 5862 3026 Size: 324 2016 496 3327 Fragments arranged by size: 3327 2016 496 324 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 2276 1163 Size: 573 401 139 5050 Fragments arranged by size: 5050 573 401 139 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4224 4309 5380 4224 Size: 85 1071 5007 Fragments arranged by size: 5007 1071 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2194 2226 2660 3304 3482 4224 4309 Size: 399 404 32 434 644 178 742 85 Cuts at: 4309 5380 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 644 434 404 399 178 85 32 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 2127 4052 5782 2127 Size: 1925 1730 2508 Fragments arranged by size: 2508 1925 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2677 3518 844 Size: 1833 841 3489 Fragments arranged by size: 3489 1833 841 BsrBI CCG'CTC Cuts at: 1394 1823 2146 4050 1394 Size: 429 323 1904 3507 Fragments arranged by size: 3507 1904 429 323 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4807 4994 1700 Size: 110 2997 187 2869 Fragments arranged by size: 2997 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 2150 1925 Size: 225 5938 BssSI C'ACGA_G Cuts at: 2210 2517 4223 5235 2210 Size: 307 1706 1012 3138 Fragments arranged by size: 3138 1706 1012 307 BstAPI GCAn_nnn'nTGC Cuts at: 794 2837 794 Size: 2043 4120 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 3493 3860 4245 4262 5714 5800 5812 6158 Size: 1521 367 385 17 1452 86 12 346 Cuts at: 6158 1972 Size: 1977 Fragments arranged by size: 1977 1521 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2852 2852 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2238 3231 513 Size: 552 1173 993 3445 Fragments arranged by size: 3445 1173 993 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 2705 2847 3679 4473 4493 2705 Size: 142 832 794 20 4375 Fragments arranged by size: 4375 832 794 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3429 3464 529 Size: 35 2865 35 3228 Fragments arranged by size: 3228 2865 35 35 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DraIII CAC_nnn'GTG Cuts at: 2187 2187 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2886 2897 Size: 54 1425 50 275 18 274 782 11 Cuts at: 2897 3610 4499 6128 8 Size: 713 889 1629 43 Fragments arranged by size: 1629 1425 889 782 713 275 274 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4093 1353 Size: 2740 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2660 2771 2834 4226 4823 5610 Size: 393 589 439 111 63 1392 597 787 Cuts at: 5610 5878 1239 Size: 268 1524 Fragments arranged by size: 1524 1392 787 597 589 439 393 268 111 63 EcoO109I rG'GnC_Cy Cuts at: 2222 2223 2848 3301 2222 Size: 1 625 453 5084 Fragments arranged by size: 5084 625 453 1 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2377 2624 3424 2377 Size: 247 800 5116 Fragments arranged by size: 5116 800 247 FspI TGC'GCA Cuts at: 2172 3069 4669 2172 Size: 897 1600 3666 Fragments arranged by size: 3666 1600 897 HaeII r_GCGC'y Cuts at: 1678 2200 5310 1678 Size: 522 3110 2531 Fragments arranged by size: 3110 2531 522 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2900 2932 3194 3226 3260 Size: 32 135 32 1328 32 262 32 34 Cuts at: 3260 3292 4804 4836 4878 4910 1373 Size: 32 1512 32 42 32 2626 Fragments arranged by size: 2626 1512 1328 262 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2011 5277 5461 1305 Size: 706 3266 184 2007 Fragments arranged by size: 3266 2007 706 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 2888 10 Size: 54 1425 343 274 782 3285 Fragments arranged by size: 3285 1425 782 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2888 4121 4480 4639 518 Size: 648 1722 1233 359 159 2042 Fragments arranged by size: 2042 1722 1233 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2834 3579 4256 5404 5649 1606 Size: 1228 745 677 1148 245 2120 Fragments arranged by size: 2120 1228 1148 745 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5611 NdeI CA'TA_TG Cuts at: 387 2017 387 Size: 1630 4533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 3521 5066 1132 Size: 97 2292 1545 2229 Fragments arranged by size: 2292 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2436 1348 Size: 1088 5075 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 2812 2895 3195 1108 Size: 1563 141 83 300 4076 Fragments arranged by size: 4076 1563 300 141 83 PfoI T'CCnGG_A Cuts at: 2736 2736 Size: 6163 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2848 2848 Size: 6163 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 2190 2222 3300 2190 Size: 32 1078 5053 Fragments arranged by size: 5053 1078 32 PspXI vC'TCGA_Gb Cuts at: 2297 2297 Size: 6163 PstI C_TGCA'G Cuts at: 830 3515 830 Size: 2685 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 2834 2834 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 3511 4646 5327 5518 826 Size: 219 2466 1135 681 191 1471 Fragments arranged by size: 2466 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2255 2255 Size: 6163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 3490 1872 Size: 1618 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 2564 2609 4267 5168 5430 Size: 197 926 354 267 45 1658 901 262 Cuts at: 5430 820 Size: 1553 Fragments arranged by size: 1658 1553 926 901 354 267 262 197 45 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 2249 2309 1517 Size: 732 60 5371 Fragments arranged by size: 5371 732 60 StyI C'CwwG_G Cuts at: 513 1065 2397 3602 513 Size: 552 1332 1205 3074 Fragments arranged by size: 3074 1332 1205 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4190 4207 4360 4545 4990 325 Size: 1255 2610 17 153 185 445 1498 Fragments arranged by size: 2610 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 4409 6121 Size: 274 80 33 51 1443 690 1742 1712 Cuts at: 6121 96 Size: 138 Fragments arranged by size: 1742 1712 1443 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2025 2057 2840 2872 6123 6155 2025 Size: 32 783 32 3251 32 2033 Fragments arranged by size: 3251 2033 783 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2972 1316 Size: 1656 4507 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XhoI C'TCGA_G Cuts at: 1943 2297 1943 Size: 354 5809 XmaI C'CCGG_G Cuts at: 1870 3488 1870 Size: 1618 4545 XmnI GAAnn'nnTTC Cuts at: 2361 3257 4292 2361 Size: 896 1035 4232 Fragments arranged by size: 4232 1035 896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI