(Circular) (Six-base) MAPSORT of: pFN21AB6410.seq Check: 9923 from: 1 to: 5710 pFN21AB6410 5710 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2404 2404 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2610 3520 278 Size: 53 83 186 936 1074 910 2468 Fragments arranged by size: 2468 1074 936 910 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3773 5157 5425 1632 Size: 896 1245 1384 268 1917 Fragments arranged by size: 1917 1384 1245 896 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2436 2848 1184 Size: 1252 412 4046 Fragments arranged by size: 4046 1252 412 AlwNI CAG_nnn'CTG Cuts at: 2195 3453 5025 2195 Size: 1258 1572 2880 Fragments arranged by size: 2880 1572 1258 ApaI G_GGCC'C Cuts at: 2243 2243 Size: 5710 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2499 3035 1870 Size: 73 556 536 4545 Fragments arranged by size: 4545 556 536 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2957 2990 2957 Size: 33 5677 BamHI G'GATC_C Cuts at: 2622 3040 3407 2622 Size: 418 367 4925 Fragments arranged by size: 4925 418 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 3031 4486 618 Size: 325 217 627 465 779 1455 1842 Fragments arranged by size: 1842 1455 779 627 465 325 217 BanII G_rGCy'C Cuts at: 1391 2243 2851 3029 1391 Size: 852 608 178 4072 Fragments arranged by size: 4072 852 608 178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2207 2241 3901 3935 1903 Size: 34 50 34 186 34 1660 34 3678 Fragments arranged by size: 3678 1660 186 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2528 3602 4812 2528 Size: 1074 1210 3426 Fragments arranged by size: 3426 1210 1074 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4321 136 Size: 107 122 71 631 605 337 2312 1525 Fragments arranged by size: 2312 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2243 2257 3771 4927 1790 Size: 453 14 1514 1156 2573 Fragments arranged by size: 2573 1514 1156 453 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 3022 4370 1239 Size: 1149 634 1348 2579 Fragments arranged by size: 2579 1348 1149 634 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2607 3517 3899 4534 Size: 53 83 186 936 1074 910 382 635 Cuts at: 4534 275 Size: 1451 Fragments arranged by size: 1451 1074 936 910 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2802 1186 Size: 1616 4094 BseYI C'CCAG_C Cuts at: 2017 2295 2913 4913 5409 2017 Size: 278 618 2000 496 2318 Fragments arranged by size: 2318 2000 618 496 278 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 BsmBI CGTCTCn'nnnn_ Cuts at: 2614 2614 Size: 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2257 2851 3029 3771 3856 4927 Size: 399 453 14 594 178 742 85 1071 Cuts at: 4927 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 594 453 399 178 85 14 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2404 3065 844 Size: 1334 226 661 3489 Fragments arranged by size: 3489 1334 661 226 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2223 2727 4205 4379 65 Size: 1183 975 504 1478 174 1396 Fragments arranged by size: 1478 1396 1183 975 504 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 4354 4541 1700 Size: 110 198 2346 187 2869 Fragments arranged by size: 2869 2346 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 2995 1184 Size: 1811 3899 BstXI CCAn_nnnn'nTGG Cuts at: 2843 2843 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2622 2686 3040 3407 3792 3809 5261 5347 Size: 650 64 354 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 650 385 367 354 346 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2682 2682 Size: 5710 BtgI C'CryG_G Cuts at: 513 1065 2087 2748 513 Size: 552 1022 661 3475 Fragments arranged by size: 3475 1022 661 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2909 487 Size: 683 56 1683 3288 Fragments arranged by size: 3288 1683 683 56 BtsI GCAGTG_nn' Cuts at: 2478 3226 4020 4040 2478 Size: 748 794 20 4148 Fragments arranged by size: 4148 794 748 20 ClaI AT'CG_AT Cuts at: 1554 2463 1554 Size: 909 4801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DraIII CAC_nnn'GTG Cuts at: 2759 2951 2759 Size: 192 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2661 3157 4046 Size: 54 1425 50 275 18 831 496 889 Cuts at: 4046 5675 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 831 496 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3640 1353 Size: 2287 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2107 4297 4671 4817 387 Size: 1064 138 518 2190 374 146 1280 Fragments arranged by size: 2190 1280 1064 518 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2528 3022 3773 4370 5157 5425 Size: 393 756 140 494 751 597 787 268 Cuts at: 5425 1239 Size: 1524 Fragments arranged by size: 1524 787 756 751 597 494 393 268 140 EcoNI CCTnn'n_nnAGG Cuts at: 2115 2174 2115 Size: 59 5651 EcoO109I rG'GnC_Cy Cuts at: 2239 2240 2773 2940 2239 Size: 1 533 167 5009 Fragments arranged by size: 5009 533 167 1 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 EcoRV GAT'ATC Cuts at: 2215 2215 Size: 5710 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2037 4857 1678 Size: 359 2820 2531 Fragments arranged by size: 2820 2531 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2145 2177 4351 4383 4425 Size: 32 135 32 573 32 2174 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 2174 573 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2663 10 Size: 54 1425 343 831 3057 Fragments arranged by size: 3057 1425 831 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 3668 4027 4186 518 Size: 648 1182 1320 359 159 2042 Fragments arranged by size: 2042 1320 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2299 3126 3803 4951 5196 1606 Size: 483 210 827 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 677 483 245 210 NaeI GCC'GGC Cuts at: 2010 2010 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 2748 513 Size: 552 1683 3475 Fragments arranged by size: 3475 1683 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PfoI T'CCnGG_A Cuts at: 2530 2530 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2940 2940 Size: 5710 PshAI GACnn'nnGTC Cuts at: 1613 2099 2526 1613 Size: 486 427 4797 Fragments arranged by size: 4797 486 427 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspOMI G'GGCC_C Cuts at: 2239 2239 Size: 5710 PspXI vC'TCGA_Gb Cuts at: 2499 2499 Size: 5710 PstI C_TGCA'G Cuts at: 830 2178 2706 3062 830 Size: 1348 528 356 3478 Fragments arranged by size: 3478 1348 528 356 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2299 2299 Size: 5710 SacII CC_GC'GG Cuts at: 2090 2090 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 2706 3062 2706 Size: 356 5354 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2174 2702 2810 2862 3058 4193 4874 Size: 219 1129 528 108 52 196 1135 681 Cuts at: 4874 5065 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1129 681 528 219 196 191 108 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2499 3814 4715 4977 820 Size: 197 926 556 1315 901 262 1553 Fragments arranged by size: 1553 1315 926 901 556 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2235 2748 3149 513 Size: 552 1170 513 401 3074 Fragments arranged by size: 3074 1170 552 513 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2240 2309 3737 3754 3907 4092 4537 Size: 1255 660 69 1428 17 153 185 445 Cuts at: 4537 325 Size: 1498 Fragments arranged by size: 1498 1428 1255 660 445 185 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2675 3956 5668 Size: 274 80 33 51 1443 698 1281 1712 Cuts at: 5668 96 Size: 138 Fragments arranged by size: 1712 1443 1281 698 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 2499 1943 Size: 556 5154 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2608 3518 276 Size: 53 83 186 936 1074 910 2468 Fragments arranged by size: 2468 1074 936 910 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI