################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AflII 900 CTTAAG C^TTAAG Asp700I 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 371 TGGCCA TGG^CCA BbsI 69 GAAGAC GAAGAC(2/6) BclI 107 TGATCA T^GATCA BfrI 900 CTTAAG C^TTAAG BpuAI 69 GAAGAC GAAGAC(2/6) BsaJI 113 CCGTGG C^C[ACGT][ACGT]GG BsgI 290 GTGCAG GTGCAG(16/14) Bsp1407I 176 TGTACA T^GTACA BspHI 401 TCATGA T^CATGA BspMI 458 ACCTGC ACCTGC(4/8) BsrDI 349 GCAATG GCAATG(2/0) BsrGI 176 TGTACA T^GTACA BsrI 588 ACTGG ACTGG(1/-1) BstF5I 725 CATCC GGATG(2/0) BstYI 757 GGATCT [GA]^GATC[CT] DraII 439 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 113 CCGTGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EcoO109I 439 GGGGCCT [GA]G^G[ACGT]CC[CT] FbaI 107 TGATCA T^GATCA FokI 725 CATCC GGATG(9/13) HindIII 707 AAGCTT A^AGCTT MaeII 776 ACGT A^CGT MflI 757 GGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspA1I 818 CAGCTG C[AC]G^C[GT]G NdeI 375 CATATG CA^TATG NlaIV 439 GGGGCC GG[ACGT]^[ACGT]CC PvuII 818 CAGCTG CAG^CTG RcaI 401 TCATGA T^CATGA SfaNI 724 GCATC GCATC(5/9) SmlI 900 CTTAAG C^T[CT][GA]AG SspBI 176 TGTACA T^GTACA SspI 124 AATATT AAT^ATT TaqI 511 TCGA T^CGA TthHB8I 511 TCGA T^CGA XhoII 757 GGATCT [GA]^GATC[CT] XmnI 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 786 AAATTT [GA]^AATT[CT] 908 AAATTC ApoI 786 AAATTT [GA]^AATT[CT] 908 AAATTC BbvI 346 GCAGC GCAGC(8/12) 820 GCTGC BsiYI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BslI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BsmI 422 GCATTC GAATGC(1/-1) 462 GCATTC Cfr13I 440 GGGCC G^G[ACGT]CC 701 GGGCC Fnu4HI 346 GCAGC GC^[ACGT]GC 820 GCTGC HphI 57 TCACC GGTGA(8/7) 110 TCACC ItaI 346 GCAGC GC^[ACGT]GC 820 GCTGC MseI 643 TTAA T^TAA 901 TTAA MwoI 346 GCAGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 442 GCCTTCATAGC PleI 72 GACTC GAGTC(4/5) 557 GACTC PstI 326 CTGCAG CTGCA^G 388 CTGCAG Sau96I 440 GGGCC G^G[ACGT]CC 701 GGGCC SfcI 326 CTGCAG C^T[GA][CT]AG 388 CTGCAG Tru9I 643 TTAA T^TAA 901 TTAA TseI 346 GCAGC G^C[AT]GC 820 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AfaI AluI AlwI DdeI DpnII HaeIII HinfI MboI MboII MnlI MslI NdeII NlaIII RsaI Sau3AI TfiI Tsp509I TspRI