Restriction map for
pFN21AB7211
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 255 GCGG CCGC(-3/-1)
AflIII 718 ACATGT A^C[GA][CT]GT
Asp700I 906 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 415 GAGCTC G[AT]GC[AT]^C
AvaII 681 GGTCC G^G[AT]CC
BalI 768 TGGCCA TGG^CCA
BamHI 698 GGATCC G^GATCC
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BciVI 638 GTATCC GTATCC(6/5)
BcnI 491 CCCGG CC^[GC]GG
BsiHKAI 415 GAGCTC G[AT]GC[AT]^C
BsmAI 744 GTCTC GTCTC(1/5)
BsmI 286 GAATGC GAATGC(1/-1)
Bsp1407I 830 TGTACA T^GTACA
BspDI 270 ATCGAT AT^CGAT
BspHI 404 TCATGA T^CATGA
BspLU11I 718 ACATGT A^CATGT
BspMI 125 ACCTGC ACCTGC(4/8)
BsrGI 830 TGTACA T^GTACA
BsrI 771 CCAGT ACTGG(1/-1)
BstXI 110 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 698 GGATCC [GA]^GATC[CT]
ClaI 270 ATCGAT AT^CGAT
DsaI 110 CCACGG C^C[GA][CT]GG
EcoNI 276 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 571 GATATC GAT^ATC
EcoT14I 900 CCTTGG C^C[AT][AT]GG
EcoT22I 288 ATGCAT ATGCA^T
MflI 698 GGATCC [GA]^GATC[CT]
MluNI 768 TGGCCA TGG^CCA
MscI 768 TGGCCA TGG^CCA
MspA1I 148 CAGCTG C[AC]G^C[GT]G
NciI 491 CCCGG CC^[GC]GG
NsiI 288 ATGCAT ATGCA^T
PleI 154 GACTC GAGTC(4/5)
PpuMI 680 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 148 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SacI 415 GAGCTC GAGCT^C
SapI 412 GAAGAGC GCTCTTC(1/4)
SfaNI 240 GATGC GCATC(5/9)
SfcI 867 CTACAG C^T[GA][CT]AG
SmlI 553 CTCAAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
SspI 762 AATATT AAT^ATT
StyI 900 CCTTGG C^C[AT][AT]GG
TaqI 271 TCGA T^CGA
TfiI 447 GATTC G^A[AT]TC
TthHB8I 271 TCGA T^CGA
XhoII 698 GGATCC [GA]^GATC[CT]
XmnI 906 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 675 CCTGAGG CC^T[ACGT]AGG
873 CCTTAGG
BanII 3 GAGCCC G[GA]GC[CT]^C
415 GAGCTC
BbsI 424 GTCTTC GAAGAC(2/6)
529 GTCTTC
BmyI 3 GAGCCC G[AGT]GC[ACT]^C
415 GAGCTC
BpuAI 424 GTCTTC GAAGAC(2/6)
529 GTCTTC
Bsp1286I 3 GAGCCC G[AGT]GC[ACT]^C
415 GAGCTC
Bsu36I 675 CCTGAGG CC^T[ACGT]AGG
873 CCTTAGG
Cac8I 69 GCAGGC GC[ACGT]^[ACGT]GC
460 GCCAGC
DpnII 273 GATC ^GATC
699 GATC
DraII 680 GGGTCCC [GA]G^G[ACGT]CC[CT]
896 AGGCCCT
DrdI 489 GACCCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
805 GACAAGGATGTC
EaeI 58 CGGCCA [CT]^GGCC[GA]
768 TGGCCA
EarI 910 CTCTTC CTCTTC(1/4)
412 GAAGAG
Eco81I 675 CCTGAGG CC^T[ACGT]AGG
873 CCTTAGG
EcoO109I 680 GGGTCCC [GA]G^G[ACGT]CC[CT]
896 AGGCCCT
HapII 57 CCGG C^CGG
492 CCGG
HinfI 154 GACTC G^A[ACGT]TC
447 GATTC
HpaII 57 CCGG C^CGG
492 CCGG
Ksp632I 910 CTCTTC CTCTTC(1/4)
412 GAAGAG
MboI 273 GATC ^GATC
699 GATC
MspI 57 CCGG C^CGG
492 CCGG
NdeII 273 GATC ^GATC
699 GATC
Sau3AI 273 GATC ^GATC
699 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeIII
MboII MnlI MslI MvaI MwoI
NdeI NlaIII NlaIV NspI RsaI
Sau96I ScrFI TseI Tsp45I TspRI