Restriction map for
pFN21AB7211
(Linear) (Six-base) MAPSORT of: KIBB7211.seq Check: 9939 from: 1 to: 939
KIBB7211.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 939
Size: 897 42
AflIII A'CryG_T
Cuts at: 0 718 939
Size: 718 221
BamHI G'GATC_C
Cuts at: 0 698 939
Size: 698 241
BanII G_rGCy'C
Cuts at: 0 7 419 939
Size: 7 412 520
Fragments arranged by size:
520 412 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 417 522 939
Size: 417 105 417
Fragments arranged by size:
417 417 105
BbvCI CC'TCA_GC
Cuts at: 0 187 939
Size: 187 752
BciVI GTATCCnnnnn_n'
Cuts at: 0 649 939
Size: 649 290
BfrBI ATG'CAT
Cuts at: 0 290 939
Size: 290 649
Bpu10I CC'TnA_GC
Cuts at: 0 187 939
Size: 187 752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 939
Size: 538 401
BseYI C'CCAG_C
Cuts at: 0 117 939
Size: 117 822
BsiHKAI G_wGCw'C
Cuts at: 0 419 939
Size: 419 520
BsmI GAATG_Cn'
Cuts at: 0 292 939
Size: 292 647
Bsp1286I G_dGCh'C
Cuts at: 0 7 419 939
Size: 7 412 520
Fragments arranged by size:
520 412 7
BspHI T'CATG_A
Cuts at: 0 404 939
Size: 404 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 939
Size: 134 805
BsrGI T'GTAC_A
Cuts at: 0 830 939
Size: 830 109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 939
Size: 117 822
BstYI r'GATC_y
Cuts at: 0 698 939
Size: 698 241
Bsu36I CC'TnA_GG
Cuts at: 0 676 874 939
Size: 676 198 65
Fragments arranged by size:
676 198 65
BtgI C'CryG_G
Cuts at: 0 110 939
Size: 110 829
ClaI AT'CG_AT
Cuts at: 0 271 939
Size: 271 668
DrdI GACnn_nn'nnGTC
Cuts at: 0 495 811 939
Size: 495 316 128
Fragments arranged by size:
495 316 128
EaeI y'GGCC_r
Cuts at: 0 58 768 939
Size: 58 710 171
Fragments arranged by size:
710 171 58
EarI CTCTTCn'nnn_
Cuts at: 0 407 916 939
Size: 407 509 23
Fragments arranged by size:
509 407 23
EcoICRI GAG'CTC
Cuts at: 0 417 939
Size: 417 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 939
Size: 897 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 939
Size: 280 659
EcoO109I rG'GnC_Cy
Cuts at: 0 681 897 939
Size: 681 216 42
Fragments arranged by size:
681 216 42
EcoRV GAT'ATC
Cuts at: 0 573 939
Size: 573 366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 916 939
Size: 916 23
MscI TGG'CCA
Cuts at: 0 770 939
Size: 770 169
MslI CAynn'nnrTG
Cuts at: 0 172 633 847 939
Size: 172 461 214 92
Fragments arranged by size:
461 214 172 92
MspA1I CmG'CkG
Cuts at: 0 150 939
Size: 150 789
NdeI CA'TA_TG
Cuts at: 0 357 376 651 939
Size: 357 19 275 288
Fragments arranged by size:
357 288 275 19
NsiI A_TGCA'T
Cuts at: 0 292 939
Size: 292 647
NspI r_CATG'y
Cuts at: 0 102 255 722 939
Size: 102 153 467 217
Fragments arranged by size:
467 217 153 102
PciI A'CATG_T
Cuts at: 0 718 939
Size: 718 221
PpuMI rG'GwC_Cy
Cuts at: 0 681 939
Size: 681 258
PvuII CAG'CTG
Cuts at: 0 150 939
Size: 150 789
SacI G_AGCT'C
Cuts at: 0 419 939
Size: 419 520
SanDI GG'GwC_CC
Cuts at: 0 681 939
Size: 681 258
SapI GCTCTTCn'nnn_
Cuts at: 0 407 939
Size: 407 532
SfcI C'TryA_G
Cuts at: 0 867 939
Size: 867 72
SmlI C'TyrA_G
Cuts at: 0 553 939
Size: 553 386
SspI AAT'ATT
Cuts at: 0 764 939
Size: 764 175
StyI C'CwwG_G
Cuts at: 0 900 939
Size: 900 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 377 861 939
Size: 377 484 78
Fragments arranged by size:
484 377 78
TatI w'GTAC_w
Cuts at: 0 176 263 830 939
Size: 176 87 567 109
Fragments arranged by size:
567 176 109 87
XmnI GAAnn'nnTTC
Cuts at: 0 910 939
Size: 910 29
Enzymes that do cut:
AcuI AflIII BamHI BanII BbsI BbvCI BciVI BfrBI
Bpu10I BpuEI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrGI BstXI BstYI Bsu36I BtgI ClaI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI MscI
MslI MspA1I NdeI NsiI NspI PciI PpuMI PvuII
SacI SanDI SapI SfcI SmlI SspI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI CspCI DraI DraIII EagI EciI EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI