Restriction map for
pFN21AB8581
(Circular) (Six-base) MAPSORT of: pFN21AB8581.seq Check: 1736 from: 1 to: 6271
pFN21AB8581 6271 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2095 2566 2594 2095
Size: 471 28 5772
Fragments arranged by size:
5772 471 28
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2755 4334 5718 5986 1632
Size: 1123 1579 1384 268 1917
Fragments arranged by size:
1917 1579 1384 1123 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 3381 5170 6147 1203
Size: 2178 1789 977 1327
Fragments arranged by size:
2178 1789 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 2447 3460 1166
Size: 1281 1013 3977
Fragments arranged by size:
3977 1281 1013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 2927 4014 5586 2927
Size: 1087 1572 3612
Fragments arranged by size:
3612 1572 1087
ApaI G_GGCC'C
Cuts at: 2038 2661 3394 2038
Size: 623 733 4915
Fragments arranged by size:
4915 733 623
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2536 2881 3580 3806 3958 6067 1493
Size: 1043 345 699 226 152 2109 1697
Fragments arranged by size:
2109 1697 1043 699 345 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3596 1870
Size: 73 1653 4545
Fragments arranged by size:
4545 1653 73
BamHI G'GATC_C
Cuts at: 2907 3601 3968 2907
Size: 694 367 5210
Fragments arranged by size:
5210 694 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2554 2606 2744 2782 2801
Size: 325 217 627 767 52 138 38 19
Cuts at: 2801 2896 3592 5047 618
Size: 95 696 1455 1842
Fragments arranged by size:
1842 1455 767 696 627 325 217 138
95 52 38 19
BanII G_rGCy'C
Cuts at: 1391 2038 2143 2661 3183 3230 3394 3590 1391
Size: 647 105 518 522 47 164 196 4072
Fragments arranged by size:
4072 647 522 518 196 164 105 47
BbeI G_GCGC'C
Cuts at: 2748 2900 2748
Size: 152 6119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3222 928
Size: 971 1323 3977
Fragments arranged by size:
3977 1323 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3082 3116 4462 4496 1903
Size: 34 1145 34 1346 34 3678
Fragments arranged by size:
3678 1346 1145 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 3012 1548
Size: 1464 4807
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4882 136
Size: 107 122 71 631 605 3210 1525
Fragments arranged by size:
3210 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3423 6266 3423
Size: 2843 3428
BlpI GC'TnA_GC
Cuts at: 2527 3224 3688 2527
Size: 697 464 5110
Fragments arranged by size:
5110 697 464
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2408 2661 3394 4332 5488 1790
Size: 248 370 253 733 938 1156 2573
Fragments arranged by size:
2573 1156 938 733 370 253 248
BmgBI CAC'GTC
Cuts at: 1318 3384 1318
Size: 2066 4205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2040 2664 4002
Size: 416 53 83 399 24 623 624 1338
Cuts at: 4002 4960 442
Size: 958 1753
Fragments arranged by size:
1753 1338 958 624 623 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2380 2439 2490 2808 3023 3044 3050
Size: 903 238 59 51 318 215 21 6
Cuts at: 3050 3254 4931 1239
Size: 204 1677 2579
Fragments arranged by size:
2579 1677 903 318 238 215 204 59
51 21 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 3320 3341 4396 5261 5559 2320
Size: 1000 21 1055 865 298 3032
Fragments arranged by size:
3032 1055 1000 865 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4934 882
Size: 617 3435 2219
Fragments arranged by size:
3435 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4062 493
Size: 711 2858 2702
Fragments arranged by size:
2858 2702 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2745 2897 4078 4460
Size: 53 83 186 936 1212 152 1181 382
Cuts at: 4460 5095 275
Size: 635 1451
Fragments arranged by size:
1451 1212 1181 936 635 382 186 152
83 53
BsaWI w'CCGG_w
Cuts at: 2510 3555 4704 5102 5376 5523 5934 2510
Size: 1045 1149 398 274 147 411 2847
Fragments arranged by size:
2847 1149 1045 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 1178
Size: 30 165 30 782 30 5234
Fragments arranged by size:
5234 782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3251 3302 3518 1186
Size: 2065 51 216 3939
Fragments arranged by size:
3939 2065 216 51
BseYI C'CCAG_C
Cuts at: 2320 2336 2350 2544 2660 2756 2912 3198 5474
Size: 16 14 194 116 96 156 286 2276
Cuts at: 5474 5970 2320
Size: 496 2621
Fragments arranged by size:
2621 2276 496 286 194 156 116 96
16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3113 1163
Size: 573 1377 4321
Fragments arranged by size:
4321 1377 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2387 3183 3375 4332 4417 5488 2387
Size: 796 192 957 85 1071 3170
Fragments arranged by size:
3170 1071 957 796 192 85
BsmI GAATG_Cn'
Cuts at: 3782 3875 3782
Size: 93 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2143 2387 2408 2661 3183 3230
Size: 399 248 105 244 21 253 522 47
Cuts at: 3230 3375 3394 3590 4332 4417 5488 1391
Size: 145 19 196 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 399 253 248 244
196 145 105 85 47 21 19
BspEI T'CCGG_A
Cuts at: 3555 5934 3555
Size: 2379 3892
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2566 2594 3228 3279 3626 844
Size: 1251 471 28 634 51 347 3489
Fragments arranged by size:
3489 1251 634 471 347 51 28
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2939 3049 3258 4158 1394
Size: 429 187 929 110 209 900 3507
Fragments arranged by size:
3507 929 900 429 209 187 110
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2174 2417 2867 3528 4915 5102 1700
Size: 110 364 243 450 661 1387 187 2869
Fragments arranged by size:
2869 1387 661 450 364 243 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6271
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 3508 1184
Size: 2324 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 2449 2449
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2907 3352 3423 3432 3601 3968 4353 4370
Size: 935 445 71 9 169 367 385 17
Cuts at: 4370 5822 5908 5920 6266 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 935 445 385 367 346 169
86 71 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2325 2550 513
Size: 552 1260 225 4234
Fragments arranged by size:
4234 1260 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2751 3413 3455 3467 3787 4581 4601 2061
Size: 690 662 42 12 320 794 20 3731
Fragments arranged by size:
3731 794 690 662 320 42 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2404 2439 2854 2889 529
Size: 35 1840 35 415 35 3911
Fragments arranged by size:
3911 1840 415 35 35 35
DraI TTT'AAA
Cuts at: 2886 3575 2886
Size: 689 5582
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2580 3718
Size: 54 1425 50 275 18 375 375 1138
Cuts at: 3718 4607 6236 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 375 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3158 4201 1353
Size: 1805 1043 3423
Fragments arranged by size:
3423 1805 1043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2971 4858 5232 5378 387
Size: 1064 138 1382 1887 374 146 1280
Fragments arranged by size:
1887 1382 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3181 3181
Size: 6271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2380 2439 2490 2755 2808 3023
Size: 393 510 238 59 51 265 53 215
Cuts at: 3023 3044 3050 3254 4334 4931 5718 5986 1239
Size: 21 6 204 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 597 510 393 268 265
238 215 204 59 53 51 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2091 3062 2091
Size: 971 5300
EcoO109I rG'GnC_Cy
Cuts at: 2034 2035 3390 3391 2034
Size: 1 1355 1 4914
Fragments arranged by size:
4914 1355 1 1
EcoRI G'AATT_C
Cuts at: 2536 3580 3958 2536
Size: 1044 378 4849
Fragments arranged by size:
4849 1044 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2184 2314 2184
Size: 130 6141
FspI TGC'GCA
Cuts at: 3444 4777 3444
Size: 1333 4938
FspAI rTGC'GCAy
Cuts at: 3444 3444
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 2748 2900 5418 1678
Size: 1070 152 2518 2531
Fragments arranged by size:
2531 2518 1070 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2703 2735 3102 3134 3461
Size: 32 135 32 1131 32 367 32 327
Cuts at: 3461 3493 4912 4944 4986 5018 1373
Size: 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1419 1131 367 327 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3079 3615 3861 669
Size: 988 281 1141 536 246 3079
Fragments arranged by size:
3079 1141 988 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KasI G'GCGC_C
Cuts at: 2744 2896 2744
Size: 152 6119
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2173 2714 5385 5569 1305
Size: 868 541 2671 184 2007
Fragments arranged by size:
2671 2007 868 541 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4449
Fragments arranged by size:
4449 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 3282 3460 4229 4588 4747 518
Size: 648 1281 835 178 769 359 159 2042
Fragments arranged by size:
2042 1281 835 769 648 359 178 159
MspA1I CmG'CkG
Cuts at: 1606 2312 2340 2621 2741 2825 2927 2960 3001
Size: 706 28 281 120 84 102 33 41
Cuts at: 3001 3687 4364 5512 5757 1606
Size: 686 677 1148 245 2120
Fragments arranged by size:
2120 1148 706 686 677 281 245 120
102 84 41 33 28
NaeI GCC'GGC
Cuts at: 3530 3530
Size: 6271
NarI GG'CG_CC
Cuts at: 2745 2897 2745
Size: 152 6119
NcoI C'CATG_G
Cuts at: 513 1065 2325 513
Size: 552 1260 4459
Fragments arranged by size:
4459 1260 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6271
NgoMIV G'CCGG_C
Cuts at: 3528 3528
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 3629 5174 1132
Size: 97 2400 1545 2229
Fragments arranged by size:
2400 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6271
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2259 4340 4372 5853 5885 2227
Size: 32 2081 32 1481 32 2613
Fragments arranged by size:
2613 2081 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PspOMI G'GGCC_C
Cuts at: 2034 2657 3390 2034
Size: 623 733 4915
Fragments arranged by size:
4915 733 623
PstI C_TGCA'G
Cuts at: 830 2965 3276 3420 3462 3492 3623 830
Size: 2135 311 144 42 30 131 3478
Fragments arranged by size:
3478 2135 311 144 131 42 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2312 2621 2927 2960 3001 2312
Size: 309 306 33 41 5582
Fragments arranged by size:
5582 309 306 41 33
SacI G_AGCT'C
Cuts at: 3183 3183
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 2653 4519 6231 2653
Size: 1866 1712 2693
Fragments arranged by size:
2693 1866 1712
SfcI C'TryA_G
Cuts at: 826 1045 2040 2091 2961 3272 3416 3458 3488
Size: 219 995 51 870 311 144 42 30
Cuts at: 3488 3619 4754 5435 5626 826
Size: 131 1135 681 191 1471
Fragments arranged by size:
1471 1135 995 870 681 311 219 191
144 131 51 42 30
SfoI GGC'GCC
Cuts at: 2746 2898 2746
Size: 152 6119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2299 3335 3356 4375 5276 5538
Size: 197 926 356 1036 21 1019 901 262
Cuts at: 5538 820
Size: 1553
Fragments arranged by size:
1553 1036 1019 926 901 356 262 197
21
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2325 3513 3710 513
Size: 552 1260 1188 197 3074
Fragments arranged by size:
3074 1260 1188 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2210 2428 2647 3086 4298 4315 4468
Size: 1255 630 218 219 439 1212 17 153
Cuts at: 4468 4653 5098 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1212 630 445 439 219 218
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2651 4517 6229
Size: 274 80 33 51 1443 674 1866 1712
Cuts at: 6229 96
Size: 138
Fragments arranged by size:
1866 1712 1443 674 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2185 2217 6231 6263 2185
Size: 32 4014 32 2193
Fragments arranged by size:
4014 2193 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2446 2323
Size: 123 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 4400 4400
Size: 6271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI