################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI TspRI TthHB8I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 9 GGTACC G^GTACC AciI 13 CCGC CCGC(-3/-1) AocI 502 CCTAAGG CC^T[ACGT]AGG Asp700I 241 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 9 GGTACC G^GTACC AspHI 262 GAGCAC G[AT]GC[AT]^C AspI 412 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 41 CCCGGG C^[CT]CG[GA]G AvaII 625 GGACC G^G[AT]CC BanI 9 GGTACC G^G[CT][GA]CC BanII 151 GAGCCC G[GA]GC[CT]^C BbvCI 646 CCTCAGC CCTCAGC(-5/-2) BciVI 471 GTATCC GTATCC(6/5) BfaI 638 CTAG C^TAG BpmI 97 CTGGAG CTGGAG(16/14) BsaI 447 GAGACC GGTCTC(1/5) BsiHKAI 262 GAGCAC G[AT]GC[AT]^C BsmFI 559 GTCCC GGGAC(10/14) BsoBI 41 CCCGGG C^[CT]CG[GA]G Bsp1407I 255 TGTACA T^GTACA BspMI 659 GCAGGT ACCTGC(4/8) BsrGI 255 TGTACA T^GTACA Bsu36I 502 CCTAAGG CC^T[ACGT]AGG Eco81I 502 CCTAAGG CC^T[ACGT]AGG HaeIII 38 GGCC GG^CC KpnI 9 GGTACC GGTAC^C MaeI 638 CTAG C^TAG MseI 370 TTAA T^TAA MspA1I 292 CAGCTG C[AC]G^C[GT]G PflFI 412 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 616 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 289 CTGCAG CTGCA^G PvuII 292 CAGCTG CAG^CTG SexAI 318 ACCTGGT A^CC[AT]GGT SmaI 41 CCCGGG CCC^GGG SspBI 255 TGTACA T^GTACA Tru9I 370 TTAA T^TAA Tsp45I 49 GTCAC ^GT[GC]AC Tth111I 412 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 616 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 41 CCCGGG C^CCGGG XmaI 41 CCCGGG C^CCGGG XmnI 241 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 208 GAATTT [GA]^AATT[CT] 453 AAATTT ApoI 208 GAATTT [GA]^AATT[CT] 453 AAATTT BcnI 41 CCCGG CC^[GC]GG 42 CCGGG BmyI 151 GAGCCC G[AGT]GC[ACT]^C 262 GAGCAC BseRI 464 CTCCTC GAGGAG(10/8) 228 GAGGAG BsgI 485 CTGCAC GTGCAG(16/14) 584 CTGCAC BsiYI 90 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 616 CCAGGATATGG BslI 90 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 616 CCAGGATATGG BsmAI 447 GAGAC GTCTC(1/5) 641 GTCTC Bsp1286I 151 GAGCCC G[AGT]GC[ACT]^C 262 GAGCAC BsrFI 31 ACCGGC [GA]^CCGG[CT] 266 ACCGGC BstF5I 72 CATCC GGATG(2/0) 396 GGATG Cac8I 23 GCTCGC GC[ACGT]^[ACGT]GC 35 GCTGGC CfoI 102 GCGC GCG^C 195 GCGC Cfr10I 31 ACCGGC [GA]^CCGG[CT] 266 ACCGGC Cfr13I 38 GGCCC G^G[ACGT]CC 625 GGACC FokI 72 CATCC GGATG(9/13) 396 GGATG HhaI 102 GCGC GCG^C 195 GCGC HinP1I 102 GCGC G^CGC 195 GCGC HphI 325 GGTGA GGTGA(8/7) 92 TCACC MwoI 78 GCAGACGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 547 GCCTTGTTTGC NciI 41 CCCGG CC^[GC]GG 42 CCGGG Sau96I 38 GGCCC G^G[ACGT]CC 625 GGACC SfcI 236 CTGTAG C^T[GA][CT]AG 289 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AfaI AluI BbvI BsaJI BsrI BssKI BstNI DdeI EcoRII Fnu4HI HapII HpaII ItaI MaeIII MboII MnlI MspI MvaI NlaIII NlaIV RsaI ScrFI TseI Tsp509I