Restriction map for
pFN21AE0448
(Circular) (Six-base) MAPSORT of: pFN21AE0448.seq Check: 331 from: 1 to: 5176
pFN21AE0448 5176 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2986 278
Size: 53 83 186 936 1450 2468
Fragments arranged by size:
2468 1450 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2519 1937
Size: 582 4594
Acc65I G'GTAC_C
Cuts at: 2038 2067 2497 2038
Size: 29 430 4717
Fragments arranged by size:
4717 430 29
AclI AA'CG_TT
Cuts at: 1563 3303 3676 1563
Size: 1740 373 3063
Fragments arranged by size:
3063 1740 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2395 3239 4623 4891 1632
Size: 763 844 1384 268 1917
Fragments arranged by size:
1917 1384 844 763 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4979
AflIII A'CryG_T
Cuts at: 1203 4075 5052 1203
Size: 2872 977 1327
Fragments arranged by size:
2872 1327 977
AgeI A'CCGG_T
Cuts at: 4007 4007
Size: 5176
AhdI GACnn_n'nnGTC
Cuts at: 3905 3905
Size: 5176
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2072
Size: 32 5144
AlwNI CAG_nnn'CTG
Cuts at: 2919 4491 2919
Size: 1572 3604
ApaLI G'TGCA_C
Cuts at: 3233 4389 3233
Size: 1156 4020
ApoI r'AATT_y
Cuts at: 1493 2485 2711 2863 4972 1493
Size: 992 226 152 2109 1697
Fragments arranged by size:
2109 1697 992 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3730 160
Size: 872 2698 1606
Fragments arranged by size:
2698 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5176
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 1870
Size: 73 558 4545
Fragments arranged by size:
4545 558 73
BamHI G'GATC_C
Cuts at: 2335 2506 2873 2335
Size: 171 367 4638
Fragments arranged by size:
4638 367 171
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2067 2104 2497 3952
Size: 325 217 627 251 29 37 393 1455
Cuts at: 3952 618
Size: 1842
Fragments arranged by size:
1842 1455 627 393 325 251 217 37
29
BanII G_rGCy'C
Cuts at: 1391 2495 1391
Size: 1104 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3367 3401 1903
Size: 34 1430 34 3678
Fragments arranged by size:
3678 1430 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3068 4278 3068
Size: 1210 3966
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5176
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3787 136
Size: 107 122 71 631 605 337 1778 1525
Fragments arranged by size:
1778 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5171 5171
Size: 5176
BlpI GC'TnA_GC
Cuts at: 2593 2593
Size: 5176
Bme1580I G_kGCm'C
Cuts at: 1790 3237 4393 1790
Size: 1447 1156 2573
Fragments arranged by size:
2573 1447 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5176
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2907 3865 442
Size: 416 53 83 399 24 1490 958 1753
Fragments arranged by size:
1753 1490 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 3836 1239
Size: 890 1707 2579
Fragments arranged by size:
2579 1707 890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2489 3301 4166 4464 2174
Size: 315 812 865 298 2886
Fragments arranged by size:
2886 865 812 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3839 882
Size: 617 2340 2219
Fragments arranged by size:
2340 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2967 493
Size: 711 1763 2702
Fragments arranged by size:
2702 1763 711
BsaBI GATnn'nnATC
Cuts at: 5170 5170
Size: 5176
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2983 3365 4000 275
Size: 53 83 186 936 1450 382 635 1451
Fragments arranged by size:
1451 1450 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3609 4007 4281 4428 4839 3609
Size: 398 274 147 411 3946
Fragments arranged by size:
3946 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 1178
Size: 30 165 30 669 30 4252
Fragments arranged by size:
4252 669 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2096 1186
Size: 850 60 4266
Fragments arranged by size:
4266 850 60
BseYI C'CCAG_C
Cuts at: 4379 4875 4379
Size: 496 4680
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4603
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2179 2626 3387 4415 1540
Size: 275 186 178 447 761 1028 2301
Fragments arranged by size:
2301 1028 761 447 275 186 178
BsiHKAI G_wGCw'C
Cuts at: 3237 3322 4393 3237
Size: 85 1071 4020
Fragments arranged by size:
4020 1071 85
BsmI GAATG_Cn'
Cuts at: 2687 2780 2687
Size: 93 5083
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2495 3237 3322 4393 1391
Size: 399 705 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 705 399 85
BspEI T'CCGG_A
Cuts at: 4839 4839
Size: 5176
BspHI T'CATG_A
Cuts at: 3065 4795 3065
Size: 1730 3446
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2531 844
Size: 1687 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 3063 1394
Size: 429 203 1037 3507
Fragments arranged by size:
3507 1037 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 3671 3845 65
Size: 1183 2423 174 1396
Fragments arranged by size:
2423 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 3820 4007 1700
Size: 110 341 1669 187 2869
Fragments arranged by size:
2869 1669 341 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5176
BssHII G'CGCG_C
Cuts at: 1925 2119 1925
Size: 194 4982
BssSI C'ACGA_G
Cuts at: 3236 4248 3236
Size: 1012 4164
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5176
BstBI TT'CG_AA
Cuts at: 3980 3980
Size: 5176
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5176
BstYI r'GATC_y
Cuts at: 1972 2184 2335 2506 2873 3258 3275 4727 4813
Size: 212 151 171 367 385 17 1452 86
Cuts at: 4813 4825 5171 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 212 171 151
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2054 513
Size: 552 989 3635
Fragments arranged by size:
3635 989 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4437
Fragments arranged by size:
4437 683 56
BtsI GCAGTG_nn'
Cuts at: 2692 3486 3506 2692
Size: 794 20 4362
Fragments arranged by size:
4362 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5176
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5141
DraI TTT'AAA
Cuts at: 2480 2480
Size: 5176
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4183 809
Size: 507 2867 1802
Fragments arranged by size:
2867 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2176 2623 3512
Size: 54 1425 50 275 18 346 447 889
Cuts at: 3512 5141 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 447 346 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2176 2623 1537
Size: 275 364 447 4090
Fragments arranged by size:
4090 447 364 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3106 1353
Size: 1753 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3763 4137 4283 387
Size: 1064 138 2174 374 146 1280
Fragments arranged by size:
2174 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2395 3239 3836 4623 4891 1239
Size: 393 497 266 844 597 787 268 1524
Fragments arranged by size:
1524 844 787 597 497 393 268 266
EcoO109I rG'GnC_Cy
Cuts at: 2049 2049
Size: 5176
EcoRI G'AATT_C
Cuts at: 2485 2863 2485
Size: 378 4798
EcoRV GAT'ATC
Cuts at: 2131 2131
Size: 5176
FspI TGC'GCA
Cuts at: 3682 3682
Size: 5176
HaeII r_GCGC'y
Cuts at: 1678 2165 4323 1678
Size: 487 2158 2531
Fragments arranged by size:
2531 2158 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 3817 3849 3891
Size: 32 135 32 837 32 1376 32 42
Cuts at: 3891 3923 1373
Size: 32 2626
Fragments arranged by size:
2626 1376 837 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2520 2766 669
Size: 988 281 582 246 3079
Fragments arranged by size:
3079 988 582 281 246
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 3558
HpaI GTT'AAC
Cuts at: 2766 2766
Size: 5176
KpnI G_GTAC'C
Cuts at: 2042 2071 2501 2042
Size: 29 430 4717
Fragments arranged by size:
4717 430 29
MfeI C'AATT_G
Cuts at: 2775 2775
Size: 5176
MluI A'CGCG_T
Cuts at: 5052 5052
Size: 5176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2282 4290 4474 1305
Size: 977 2008 184 2007
Fragments arranged by size:
2008 2007 977 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3354
Fragments arranged by size:
3354 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3134 3493 3652 518
Size: 648 1968 359 159 2042
Fragments arranged by size:
2042 1968 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2333 2592 3269 4417 4662 1606
Size: 727 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 727 677 259 245
NaeI GCC'GGC
Cuts at: 2153 2153
Size: 5176
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4624
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5176
NgoMIV G'CCGG_C
Cuts at: 2151 2151
Size: 5176
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5176
NotI GC'GGCC_GC
Cuts at: 2176 2623 2176
Size: 447 4729
NspI r_CATG'y
Cuts at: 1132 1229 2159 2534 4079 1132
Size: 97 930 375 1545 2229
Fragments arranged by size:
2229 1545 930 375 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5176
PciI A'CATG_T
Cuts at: 4075 4075
Size: 5176
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 1108
Size: 969 4207
PmeI GTTT'AAAC
Cuts at: 2480 2480
Size: 5176
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2072 2104 3245 3277 4758 4790 2072
Size: 32 1141 32 1481 32 2458
Fragments arranged by size:
2458 1481 1141 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2049 2049
Size: 5176
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5176
PsiI TTA'TAA
Cuts at: 2746 2746
Size: 5176
PstI C_TGCA'G
Cuts at: 830 2528 830
Size: 1698 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5176
SalI G'TCGA_C
Cuts at: 1936 2518 1936
Size: 582 4594
SanDI GG'GwC_CC
Cuts at: 2049 2049
Size: 5176
SbfI CC_TGCA'GG
Cuts at: 2528 2528
Size: 5176
ScaI AGT'ACT
Cuts at: 3424 5136 3424
Size: 1712 3464
SfcI C'TryA_G
Cuts at: 826 1045 2524 3659 4340 4531 826
Size: 219 1479 1135 681 191 1471
Fragments arranged by size:
1479 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5176
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5176
SmaI CCC'GGG
Cuts at: 1872 2503 1872
Size: 631 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 2468 3280 4181 4443 820
Size: 197 926 246 279 812 901 262 1553
Fragments arranged by size:
1553 926 901 812 279 262 246 197
SnaBI TAC'GTA
Cuts at: 493 2967 493
Size: 2474 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5176
SphI G_CATG'C
Cuts at: 1132 2534 1132
Size: 1402 3774
SspI AAT'ATT
Cuts at: 5 52 3100 5
Size: 47 3048 2081
Fragments arranged by size:
3048 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5176
StyI C'CwwG_G
Cuts at: 513 1065 2076 2339 2615 513
Size: 552 1011 263 276 3074
Fragments arranged by size:
3074 1011 552 276 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2332 3203 3220 3373 3558 4003 325
Size: 1255 752 871 17 153 185 445 1498
Fragments arranged by size:
1498 1255 871 752 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3422 5134 96
Size: 274 80 33 51 1443 1445 1712 138
Fragments arranged by size:
1712 1445 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2120 2152 5136 5168 2120
Size: 32 2984 32 2128
Fragments arranged by size:
2984 2128 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5176
XbaI T'CTAG_A
Cuts at: 2512 2512
Size: 5176
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5176
XmaI C'CCGG_G
Cuts at: 1870 2501 1870
Size: 631 4545
XmnI GAAnn'nnTTC
Cuts at: 3305 3305
Size: 5176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2984 276
Size: 53 83 186 936 1450 2468
Fragments arranged by size:
2468 1450 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI