(Circular) (Six-base) MAPSORT of: pFN21AB7803.seq Check: 3425 from: 1 to: 6214 pFN21AB7803 6214 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3415 3415 Size: 6214 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2730 2991 4277 5661 5929 1632 Size: 1098 261 1286 1384 268 1917 Fragments arranged by size: 1917 1384 1286 1098 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 2330 5113 6090 1203 Size: 1127 2783 977 1327 Fragments arranged by size: 2783 1327 1127 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 2506 3290 4943 2506 Size: 784 1653 3777 Fragments arranged by size: 3777 1653 784 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2195 2231 2591 3957 5529 2195 Size: 36 360 1366 1572 2880 Fragments arranged by size: 2880 1572 1366 360 36 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 2580 3523 3749 3901 6010 1493 Size: 1087 943 226 152 2109 1697 Fragments arranged by size: 2109 1697 1087 943 226 152 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2147 3539 1870 Size: 73 204 1392 4545 Fragments arranged by size: 4545 1392 204 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3535 4990 618 Size: 325 217 627 1748 1455 1842 Fragments arranged by size: 1842 1748 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3533 1391 Size: 2142 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 2505 2838 928 Size: 971 237 369 333 4304 Fragments arranged by size: 4304 971 369 333 237 BbvCI CC'TCA_GC Cuts at: 3261 3261 Size: 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2996 3143 3173 Size: 107 122 71 631 605 1324 147 30 Cuts at: 3173 3257 4825 136 Size: 84 1568 1525 Fragments arranged by size: 1568 1525 1324 631 605 147 122 107 84 71 30 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 4275 5431 1790 Size: 2485 1156 2573 Fragments arranged by size: 2573 2485 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3945 4903 442 Size: 416 53 83 399 24 2528 958 1753 Fragments arranged by size: 2528 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2566 2173 Size: 393 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2525 3354 4874 1239 Size: 1286 829 1520 2579 Fragments arranged by size: 2579 1520 1286 829 Bpu10I CC'TnA_GC Cuts at: 3261 3261 Size: 6214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 4339 5204 5502 2627 Size: 1712 865 298 3339 Fragments arranged by size: 3339 1712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4877 882 Size: 617 3378 2219 Fragments arranged by size: 3378 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 3110 6208 3110 Size: 3098 3116 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2313 2733 4647 5045 5319 5466 5877 2313 Size: 420 1914 398 274 147 411 2650 Fragments arranged by size: 2650 1914 420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3192 3222 1178 Size: 30 165 30 1789 30 4170 Fragments arranged by size: 4170 1789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6214 BseYI C'CCAG_C Cuts at: 2067 2613 2673 5417 5913 2067 Size: 546 60 2744 496 2368 Fragments arranged by size: 2744 2368 546 496 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 2469 3443 1163 Size: 573 360 373 974 3934 Fragments arranged by size: 3934 974 573 373 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3328 4275 4360 5431 3328 Size: 947 85 1071 4111 Fragments arranged by size: 4111 1071 947 85 BsmI GAATG_Cn' Cuts at: 3725 3818 3725 Size: 93 6121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3328 3533 4275 4360 5431 1391 Size: 399 1538 205 742 85 1071 2174 Fragments arranged by size: 2174 1538 1071 742 399 205 85 BspEI T'CCGG_A Cuts at: 2313 2733 5877 2313 Size: 420 3144 2650 Fragments arranged by size: 3144 2650 420 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 2216 3415 3569 844 Size: 1227 145 1199 154 3489 Fragments arranged by size: 3489 1227 1199 154 145 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4709 4883 65 Size: 1183 3461 174 1396 Fragments arranged by size: 3461 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 2376 4274 5286 2376 Size: 1898 1012 3304 Fragments arranged by size: 3304 1898 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2113 794 Size: 1319 4895 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 3025 3391 1184 Size: 1841 366 4007 Fragments arranged by size: 4007 1841 366 BstYI r'GATC_y Cuts at: 1972 3363 3544 3911 4296 4313 5765 5851 5863 Size: 1391 181 367 385 17 1452 86 12 Cuts at: 5863 6209 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1391 385 367 346 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3021 3054 3432 3474 513 Size: 552 939 1017 33 378 42 3253 Fragments arranged by size: 3253 1017 939 552 378 42 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2067 3177 3730 4524 4544 2067 Size: 1110 553 794 20 3737 Fragments arranged by size: 3737 1110 794 553 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 2437 3518 2437 Size: 1081 5133 DraIII CAC_nnn'GTG Cuts at: 2020 2020 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3467 5221 809 Size: 507 2151 1754 1802 Fragments arranged by size: 2151 1802 1754 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 3429 3481 Size: 54 1425 50 275 18 411 1188 52 Cuts at: 3481 3661 4550 6179 8 Size: 180 889 1629 43 Fragments arranged by size: 1629 1425 1188 889 411 275 180 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 2748 4144 1353 Size: 959 436 1396 3423 Fragments arranged by size: 3423 1396 959 436 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2525 2730 2991 3354 4277 4874 5661 Size: 393 893 205 261 363 923 597 787 Cuts at: 5661 5929 1239 Size: 268 1524 Fragments arranged by size: 1524 923 893 787 597 393 363 268 261 205 EcoNI CCTnn'n_nnAGG Cuts at: 3260 3404 3260 Size: 144 6070 EcoO109I rG'GnC_Cy Cuts at: 2228 2228 Size: 6214 EcoRI G'AATT_C Cuts at: 2580 3523 3901 2580 Size: 943 378 4893 Fragments arranged by size: 4893 943 378 EcoRV GAT'ATC Cuts at: 2716 3456 2716 Size: 740 5474 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 3221 5361 1678 Size: 1543 2140 2531 Fragments arranged by size: 2531 2140 1543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2197 2229 2492 Size: 32 135 32 545 32 48 32 263 Cuts at: 2492 2524 3192 3224 3276 3308 4855 4887 4929 Size: 32 668 32 52 32 1547 32 42 Cuts at: 4929 4961 1373 Size: 32 2626 Fragments arranged by size: 2626 1547 668 545 263 135 52 48 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2548 3558 3804 669 Size: 988 281 610 1010 246 3079 Fragments arranged by size: 3079 1010 988 610 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 2548 3804 2548 Size: 1256 4958 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3301 5328 5512 1305 Size: 1996 2027 184 2007 Fragments arranged by size: 2027 2007 1996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3431 10 Size: 54 1425 343 1599 2793 Fragments arranged by size: 2793 1599 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4172 4531 4690 518 Size: 648 3006 359 159 2042 Fragments arranged by size: 3006 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2195 2222 2246 3630 4307 5455 5700 1606 Size: 589 27 24 1384 677 1148 245 2120 Fragments arranged by size: 2120 1384 1148 677 589 245 27 24 NcoI C'CATG_G Cuts at: 513 1065 2004 3432 3474 513 Size: 552 939 1428 42 3253 Fragments arranged by size: 3253 1428 939 552 42 NdeI CA'TA_TG Cuts at: 387 2037 387 Size: 1650 4564 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 2060 3379 3572 5117 1132 Size: 97 831 1319 193 1545 2229 Fragments arranged by size: 2229 1545 1319 831 193 97 PasI CC'CwG_GG Cuts at: 1348 2721 3406 1348 Size: 1373 685 4156 Fragments arranged by size: 4156 1373 685 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2703 3148 3388 3490 1108 Size: 1595 445 240 102 3832 Fragments arranged by size: 3832 1595 445 240 102 PfoI T'CCnGG_A Cuts at: 2493 2493 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 2228 Size: 6214 PshAI GACnn'nnGTC Cuts at: 1613 2921 1613 Size: 1308 4906 PsiI TTA'TAA Cuts at: 2814 3784 2814 Size: 970 5244 PstI C_TGCA'G Cuts at: 830 2227 3566 830 Size: 1397 1339 3478 Fragments arranged by size: 3478 1397 1339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2195 2222 2195 Size: 27 6187 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SapI GCTCTTCn'nnn_ Cuts at: 2748 2748 Size: 6214 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 2648 4462 6174 2648 Size: 1814 1712 2688 Fragments arranged by size: 2688 1814 1712 SfcI C'TryA_G Cuts at: 826 1045 2223 2652 2919 3204 3562 4697 5378 Size: 219 1178 429 267 285 358 1135 681 Cuts at: 5378 5569 826 Size: 191 1471 Fragments arranged by size: 1471 1178 1135 681 429 358 285 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2642 4318 5219 5481 820 Size: 197 926 699 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 699 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6214 StyI C'CwwG_G Cuts at: 513 1065 2004 3432 3474 3653 513 Size: 552 939 1428 42 179 3074 Fragments arranged by size: 3074 1428 939 552 179 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3155 4241 4258 4411 4596 5041 325 Size: 1255 1575 1086 17 153 185 445 1498 Fragments arranged by size: 1575 1498 1255 1086 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 2646 4460 Size: 274 80 33 51 1443 501 168 1814 Cuts at: 4460 6172 96 Size: 1712 138 Fragments arranged by size: 1814 1712 1443 501 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6214 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3145 3347 3145 Size: 202 6012 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 4343 4343 Size: 6214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI