(Circular) (Six-base) MAPSORT of: pFN21AB7838.seq Check: 4885 from: 1 to: 6547 pFN21AB7838 6547 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3025 3217 3025 Size: 192 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2311 3890 1937 Size: 374 1579 4594 Fragments arranged by size: 4594 1579 374 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2534 3556 3749 4610 5994 6262 1632 Size: 902 1022 193 861 1384 268 1917 Fragments arranged by size: 1917 1384 1022 902 861 268 193 AflII C'TTAA_G Cuts at: 820 1017 3419 820 Size: 197 2402 3948 Fragments arranged by size: 3948 2402 197 AflIII A'CryG_T Cuts at: 1203 3180 3267 5446 6423 1203 Size: 1977 87 2179 977 1327 Fragments arranged by size: 2179 1977 1327 977 87 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 2436 3179 3629 1166 Size: 1270 743 450 4084 Fragments arranged by size: 4084 1270 743 450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6547 AlwNI CAG_nnn'CTG Cuts at: 2134 4290 5862 2134 Size: 2156 1572 2819 Fragments arranged by size: 2819 2156 1572 ApaI G_GGCC'C Cuts at: 2759 2890 2759 Size: 131 6416 ApaLI G'TGCA_C Cuts at: 2441 4604 5760 2441 Size: 2163 1156 3228 Fragments arranged by size: 3228 2163 1156 ApoI r'AATT_y Cuts at: 1493 3667 3856 4082 4234 6343 1493 Size: 2174 189 226 152 2109 1697 Fragments arranged by size: 2174 2109 1697 226 189 152 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 2422 2601 2883 3376 3655 3872 1870 Size: 73 479 179 282 493 279 217 4545 Fragments arranged by size: 4545 493 479 282 279 217 179 73 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3159 3205 3428 3530 3868 Size: 325 217 627 1372 46 223 102 338 Cuts at: 3868 5323 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1372 627 338 325 223 217 102 46 BanII G_rGCy'C Cuts at: 1391 2300 2759 2853 2890 3712 3721 3866 1391 Size: 909 459 94 37 822 9 145 4072 Fragments arranged by size: 4072 909 822 459 145 94 37 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3636 3670 4738 4772 1903 Size: 34 1699 34 1068 34 3678 Fragments arranged by size: 3678 1699 1068 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 1548 Size: 6547 BfrBI ATG'CAT Cuts at: 2960 3808 2960 Size: 848 5699 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2754 5158 136 Size: 107 122 71 631 605 1082 2404 1525 Fragments arranged by size: 2404 1525 1082 631 605 122 107 71 BglII A'GATC_T Cuts at: 2898 3306 6542 2898 Size: 408 3236 2903 Fragments arranged by size: 3236 2903 408 BlpI GC'TnA_GC Cuts at: 3964 3964 Size: 6547 Bme1580I G_kGCm'C Cuts at: 1790 2445 2711 2759 2890 3162 4608 5764 1790 Size: 655 266 48 131 272 1446 1156 2573 Fragments arranged by size: 2573 1446 1156 655 272 266 131 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2800 4278 5236 Size: 416 53 83 399 24 1383 1478 958 Cuts at: 5236 442 Size: 1753 Fragments arranged by size: 1753 1478 1383 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2545 3337 3569 2545 Size: 792 232 5523 Fragments arranged by size: 5523 792 232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2735 2958 3674 5207 1239 Size: 805 691 223 716 1533 2579 Fragments arranged by size: 2579 1533 805 716 691 223 Bpu10I CC'TnA_GC Cuts at: 2847 3497 2847 Size: 650 5897 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3758 4672 5537 5835 3758 Size: 914 865 298 4470 Fragments arranged by size: 4470 914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3540 5210 882 Size: 617 2041 1670 2219 Fragments arranged by size: 2219 2041 1670 617 BsaAI yAC'GTr Cuts at: 493 1204 3181 4338 493 Size: 711 1977 1157 2702 Fragments arranged by size: 2702 1977 1157 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2825 3840 4354 Size: 53 83 186 936 507 785 1015 514 Cuts at: 4354 4736 5371 275 Size: 382 635 1451 Fragments arranged by size: 1451 1015 936 785 635 514 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2776 4980 5378 5652 5799 6210 2776 Size: 2204 398 274 147 411 3113 Fragments arranged by size: 3113 2204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 3097 3127 1178 Size: 30 165 30 639 30 1025 30 4598 Fragments arranged by size: 4598 1025 639 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 2266 2269 3082 3620 3623 1186 Size: 873 207 3 813 538 3 4110 Fragments arranged by size: 4110 873 813 538 207 3 3 BseYI C'CCAG_C Cuts at: 2222 2348 2730 2923 2994 3048 3613 5750 6246 Size: 126 382 193 71 54 565 2137 496 Cuts at: 6246 2222 Size: 2523 Fragments arranged by size: 2523 2137 565 496 382 193 126 71 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3108 3320 1163 Size: 573 1372 212 4390 Fragments arranged by size: 4390 1372 573 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3997 4758 5786 1540 Size: 275 186 1996 761 1028 2301 Fragments arranged by size: 2301 1996 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2360 2445 4608 4693 5764 2360 Size: 85 2163 85 1071 3143 Fragments arranged by size: 3143 2163 1071 85 85 BsmI GAATG_Cn' Cuts at: 2214 3808 4058 4151 2214 Size: 1594 250 93 4610 Fragments arranged by size: 4610 1594 250 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2598 3689 2598 Size: 1091 5456 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2300 2360 2445 2711 2759 2853 2890 Size: 399 510 60 85 266 48 94 37 Cuts at: 2890 3162 3712 3721 3866 4608 4693 5764 1391 Size: 272 550 9 145 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 510 399 272 266 145 94 85 85 60 48 37 9 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 4436 6166 4436 Size: 1730 4817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3025 3217 3558 3795 3902 844 Size: 2181 192 341 237 107 3489 Fragments arranged by size: 3489 2181 341 237 192 107 BsrBI CCG'CTC Cuts at: 1394 1823 3654 4434 1394 Size: 429 1831 780 3507 Fragments arranged by size: 3507 1831 780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2570 5042 5216 65 Size: 1183 1322 2472 174 1396 Fragments arranged by size: 2472 1396 1322 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3735 5191 5378 1700 Size: 110 1925 1456 187 2869 Fragments arranged by size: 2869 1925 1456 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 2556 2650 2706 794 Size: 1762 94 56 4635 Fragments arranged by size: 4635 1762 94 56 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstXI CCAn_nnnn'nTGG Cuts at: 2680 2994 3541 2680 Size: 314 547 5686 Fragments arranged by size: 5686 547 314 BstYI r'GATC_y Cuts at: 1972 2834 2898 3306 3877 4244 4629 4646 6098 Size: 862 64 408 571 367 385 17 1452 Cuts at: 6098 6184 6196 6542 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 862 571 408 385 367 346 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2322 2751 3534 3627 513 Size: 552 1257 429 783 93 3433 Fragments arranged by size: 3433 1257 783 552 429 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2975 487 Size: 683 56 1749 4059 Fragments arranged by size: 4059 1749 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4063 4857 4877 3292 Size: 771 794 20 4962 Fragments arranged by size: 4962 794 771 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2017 2052 529 Size: 35 1453 35 5024 Fragments arranged by size: 5024 1453 35 35 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2682 3994 Size: 54 1425 50 275 18 489 363 1312 Cuts at: 3994 4883 6512 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1312 889 489 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3626 3717 4477 1353 Size: 2273 91 760 3423 Fragments arranged by size: 3423 2273 760 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2534 2735 2958 3556 3674 3749 Size: 393 412 490 201 223 598 118 75 Cuts at: 3749 4610 5207 5994 6262 1239 Size: 861 597 787 268 1524 Fragments arranged by size: 1524 861 787 598 597 490 412 393 268 223 201 118 75 EcoNI CCTnn'n_nnAGG Cuts at: 3419 3419 Size: 6547 EcoO109I rG'GnC_Cy Cuts at: 2747 2756 3064 2747 Size: 9 308 6230 Fragments arranged by size: 6230 308 9 EcoRI G'AATT_C Cuts at: 3667 3856 4234 3667 Size: 189 378 5980 Fragments arranged by size: 5980 378 189 EcoRV GAT'ATC Cuts at: 2871 2871 Size: 6547 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 3599 5694 1678 Size: 1921 2095 2531 Fragments arranged by size: 2531 2095 1921 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2405 2437 2855 Size: 32 135 32 653 32 148 32 418 Cuts at: 2855 2887 5188 5220 5262 5294 1373 Size: 32 2301 32 42 32 2626 Fragments arranged by size: 2626 2301 653 418 148 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 2456 3171 5661 5845 1305 Size: 741 410 715 2490 184 2007 Fragments arranged by size: 2490 2007 741 715 410 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 2684 10 Size: 54 1425 343 489 363 3873 Fragments arranged by size: 3873 1425 489 363 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 3179 3183 3539 3629 4505 4864 Size: 648 1270 743 4 356 90 876 359 Cuts at: 4864 5023 518 Size: 159 2042 Fragments arranged by size: 2042 1270 876 743 648 359 356 159 90 4 MspA1I CmG'CkG Cuts at: 1606 2556 2647 2713 2821 3135 3341 3629 3963 Size: 950 91 66 108 314 206 288 334 Cuts at: 3963 4640 5788 6033 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 677 334 314 288 245 206 108 91 66 NcoI C'CATG_G Cuts at: 513 1065 2322 2751 3534 513 Size: 552 1257 429 783 3526 Fragments arranged by size: 3526 1257 783 552 429 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NsiI A_TGCA'T Cuts at: 2962 3810 2962 Size: 848 5699 NspI r_CATG'y Cuts at: 1132 1229 2599 3258 3271 3808 3905 5450 1132 Size: 97 1370 659 13 537 97 1545 2229 Fragments arranged by size: 2229 1545 1370 659 537 97 97 13 PasI CC'CwG_GG Cuts at: 1348 2303 1348 Size: 955 5592 PciI A'CATG_T Cuts at: 3267 5446 3267 Size: 2179 4368 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6547 PfoI T'CCnGG_A Cuts at: 3690 3690 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PmlI CAC'GTG Cuts at: 3181 3181 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 2755 2886 2755 Size: 131 6416 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2483 2515 2483 Size: 32 6515 PstI C_TGCA'G Cuts at: 830 3522 3649 3899 830 Size: 2692 127 250 3478 Fragments arranged by size: 3478 2692 250 127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 2821 3135 2821 Size: 314 6233 SacII CC_GC'GG Cuts at: 3630 3630 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SapI GCTCTTCn'nnn_ Cuts at: 3717 3717 Size: 6547 SbfI CC_TGCA'GG Cuts at: 3522 3899 3522 Size: 377 6170 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SfcI C'TryA_G Cuts at: 826 1045 2101 3511 3518 3645 3895 5030 5711 Size: 219 1056 1410 7 127 250 1135 681 Cuts at: 5711 5902 826 Size: 191 1471 Fragments arranged by size: 1471 1410 1135 1056 681 250 219 191 127 7 SfiI GGCCn_nnn'nGGCC Cuts at: 2754 2754 Size: 6547 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 2885 3378 3874 1872 Size: 1013 493 496 4545 Fragments arranged by size: 4545 1013 496 493 SmlI C'TyrA_G Cuts at: 820 1017 1943 2601 3419 3773 4651 5552 5814 Size: 197 926 658 818 354 878 901 262 Cuts at: 5814 820 Size: 1553 Fragments arranged by size: 1553 926 901 878 818 658 354 262 197 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SrfI GCCC'GGGC Cuts at: 2885 2885 Size: 6547 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 2140 2322 2751 3039 3534 3741 3986 Size: 552 1075 182 429 288 495 207 245 Cuts at: 3986 513 Size: 3074 Fragments arranged by size: 3074 1075 552 495 429 288 245 207 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 2236 2740 2744 3191 4574 4591 Size: 1255 621 35 504 4 447 1383 17 Cuts at: 4591 4744 4929 5374 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1383 1255 621 504 447 445 185 153 35 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2499 4793 6505 Size: 274 80 33 51 1443 522 2294 1712 Cuts at: 6505 96 Size: 138 Fragments arranged by size: 2294 1712 1443 522 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3097 3129 6507 6539 3097 Size: 32 3378 32 3105 Fragments arranged by size: 3378 3105 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3407 1316 Size: 2091 4456 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 2601 1943 Size: 658 5889 XmaI C'CCGG_G Cuts at: 1870 2883 3376 3872 1870 Size: 1013 493 496 4545 Fragments arranged by size: 4545 1013 496 493 XmnI GAAnn'nnTTC Cuts at: 4676 4676 Size: 6547 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI RsrII SacI SanDI SexAI SfoI SwaI