Restriction map for
pFN21AB7878
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1805 GTCTAC GT^[AC][GT]AC
AcsI 1831 GAATTT [GA]^AATT[CT]
AflIII 653 ACATGT A^C[GA][CT]GT
AhdI 436 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1811 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1831 GAATTT [GA]^AATT[CT]
AspEI 436 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 595 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 100 GCAAACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1728 CCGGG CC^[GC]GG
BglII 90 AGATCT A^GATCT
BsaI 1350 GAGACC GGTCTC(1/5)
BsgI 803 GTGCAG GTGCAG(16/14)
BspLU11I 653 ACATGT A^CATGT
BspMI 790 ACCTGC ACCTGC(4/8)
BsrDI 1496 GCAATG GCAATG(2/0)
BsrFI 743 GCCGGC [GA]^CCGG[CT]
BstBI 50 TTCGAA TT^CGAA
BstXI 1354 CCATCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 743 GCCGGC [GA]^CCGG[CT]
DraI 874 TTTAAA TTT^AAA
Eam1105I 436 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1570 CCAAGG C^C[AT][AT]GG
FauI 862 CCCGC CCCGC(4/6)
HincII 1837 GTTAAC GT[CT]^[GA]AC
HindII 1837 GTTAAC GT[CT]^[GA]AC
HindIII 1774 AAGCTT A^AGCTT
HpaI 1837 GTTAAC GTT^AAC
NaeI 743 GCCGGC GCC^GGC
NciI 1728 CCGGG CC^[GC]GG
NgoMIV 743 GCCGGC G^CCGGC
NspI 653 ACATGT [GA]CATG^[CT]
NspV 50 TTCGAA TT^CGAA
PflFI 595 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1810 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 997 GAAGAGC GCTCTTC(1/4)
SfuI 50 TTCGAA TT^CGAA
StuI 1881 AGGCCT AGG^CCT
StyI 1570 CCAAGG C^C[AT][AT]GG
TaqI 51 TCGA T^CGA
Tth111I 595 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 51 TCGA T^CGA
Van91I 1810 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 912 CCAGGAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 965 GTAC GT^AC
1732 GTAC
AspHI 84 GAGCAC G[AT]GC[AT]^C
1000 GAGCAC
BamHI 27 GGATCC G^GATCC
111 GGATCC
BbvCI 4 CCTCAGC CCTCAGC(-5/-2)
577 GCTGAGG
BciVI 273 GGATAC GTATCC(6/5)
1898 GGATAC
BmyI 84 GAGCAC G[AGT]GC[ACT]^C
1000 GAGCAC
BsiHKAI 84 GAGCAC G[AT]GC[AT]^C
1000 GAGCAC
BsmFI 583 GTCCC GGGAC(10/14)
902 GTCCC
Bsp1286I 84 GAGCAC G[AGT]GC[ACT]^C
1000 GAGCAC
HapII 744 CCGG C^CGG
1728 CCGG
HpaII 744 CCGG C^CGG
1728 CCGG
MslI 766 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1355 CATCTGAGTG
MspA1I 315 CAGCTG C[AC]G^C[GT]G
542 CAGCTG
MspI 744 CCGG C^CGG
1728 CCGG
MwoI 856 GCCACTCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1600 GCTTCCAGAGC
PshAI 1536 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1568 GACCAAGGTC
PstI 571 CTGCAG CTGCA^G
1859 CTGCAG
PvuII 315 CAGCTG CAG^CTG
542 CAGCTG
RsaI 965 GTAC GT^AC
1732 GTAC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AvaII BbsI
BbvI BfaI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII