(Circular) (Six-base) MAPSORT of: pFN21AB7878.seq Check: 4032 from: 1 to: 6622 pFN21AB7878 6622 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2804 2804 Size: 6622 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3811 3965 1937 Size: 1874 154 4594 Fragments arranged by size: 4594 1874 154 Acc65I G'GTAC_C Cuts at: 3943 3943 Size: 6622 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3087 3275 4685 6069 6337 1632 Size: 1455 188 1410 1384 268 1917 Fragments arranged by size: 1917 1455 1410 1384 268 188 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 2658 5521 6498 1203 Size: 1455 2863 977 1327 Fragments arranged by size: 2863 1455 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 2446 5351 2446 Size: 2905 3717 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6622 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 3821 4365 5937 3821 Size: 544 1572 4506 Fragments arranged by size: 4506 1572 544 ApaLI G'TGCA_C Cuts at: 4679 5835 4679 Size: 1156 5466 ApoI r'AATT_y Cuts at: 1493 3836 3931 4157 4309 6418 1493 Size: 2343 95 226 152 2109 1697 Fragments arranged by size: 2343 2109 1697 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 3947 1870 Size: 73 2004 4545 Fragments arranged by size: 4545 2004 73 BamHI G'GATC_C Cuts at: 2032 2116 3952 4319 2032 Size: 84 1836 367 4335 Fragments arranged by size: 4335 1836 367 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3943 5398 618 Size: 325 217 627 2156 1455 1842 Fragments arranged by size: 2156 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3941 1391 Size: 2550 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2298 2657 3255 3468 3866 928 Size: 971 399 359 598 213 398 3684 Fragments arranged by size: 3684 971 598 399 398 359 213 BbvCI CC'TCA_GC Cuts at: 2010 2583 2010 Size: 573 6049 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2094 2128 4813 4847 1903 Size: 34 50 34 73 34 2685 34 3678 Fragments arranged by size: 3678 2685 73 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 3897 4514 5724 2272 Size: 1625 617 1210 3170 Fragments arranged by size: 3170 1625 1210 617 BclI T'GATC_A Cuts at: 1548 1548 Size: 6622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5233 136 Size: 107 122 71 631 605 3561 1525 Fragments arranged by size: 3561 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2095 6617 2095 Size: 4522 2100 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 4683 5839 1790 Size: 2893 1156 2573 Fragments arranged by size: 2893 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 2729 3631 Size: 416 53 83 399 24 858 454 902 Cuts at: 3631 4353 5311 442 Size: 722 958 1753 Fragments arranged by size: 1753 958 902 858 722 454 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 3476 3528 3903 5282 1239 Size: 998 1239 52 375 1379 2579 Fragments arranged by size: 2579 1379 1239 998 375 52 Bpu10I CC'TnA_GC Cuts at: 2010 2583 2010 Size: 573 6049 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4747 5612 5910 4747 Size: 865 298 5459 Fragments arranged by size: 5459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3349 5285 882 Size: 617 1850 1936 2219 Fragments arranged by size: 2219 1936 1850 617 BsaAI yAC'GTr Cuts at: 493 1204 4413 493 Size: 711 3209 2702 Fragments arranged by size: 3209 2702 711 BsaBI GATnn'nnATC Cuts at: 6616 6616 Size: 6622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4429 4811 5446 275 Size: 53 83 186 936 2896 382 635 1451 Fragments arranged by size: 2896 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5055 5453 5727 5874 6285 5055 Size: 398 274 147 411 5392 Fragments arranged by size: 5392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3876 3906 1178 Size: 30 165 30 2473 30 3894 Fragments arranged by size: 3894 2473 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3092 3101 3167 3170 3479 1186 Size: 1906 9 66 3 309 4329 Fragments arranged by size: 4329 1906 309 66 9 3 BseYI C'CCAG_C Cuts at: 3821 5825 6321 3821 Size: 2004 496 4122 Fragments arranged by size: 4122 2004 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2829 1163 Size: 573 1093 4956 Fragments arranged by size: 4956 1093 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4072 4833 5861 1540 Size: 275 186 2071 761 1028 2301 Fragments arranged by size: 2301 2071 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2093 3009 4683 4768 5839 2093 Size: 916 1674 85 1071 2876 Fragments arranged by size: 2876 1674 1071 916 85 BsmI GAATG_Cn' Cuts at: 4133 4226 4133 Size: 93 6529 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 3009 3941 4683 4768 5839 1391 Size: 399 303 916 932 742 85 1071 2174 Fragments arranged by size: 2174 1071 932 916 742 399 303 85 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 4511 6241 4511 Size: 1730 4892 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2804 3977 844 Size: 1960 1173 3489 Fragments arranged by size: 3489 1960 1173 BsrBI CCG'CTC Cuts at: 1394 1823 4509 1394 Size: 429 2686 3507 Fragments arranged by size: 3507 2686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3508 5117 5291 65 Size: 1183 2260 1609 174 1396 Fragments arranged by size: 2260 1609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2748 5266 5453 1700 Size: 110 938 2518 187 2869 Fragments arranged by size: 2869 2518 938 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 4682 5694 4682 Size: 1012 5610 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6622 BstBI TT'CG_AA Cuts at: 2056 5426 2056 Size: 3370 3252 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6622 BstXI CCAn_nnnn'nTGG Cuts at: 3366 3366 Size: 6622 BstYI r'GATC_y Cuts at: 1972 2032 2095 2116 3952 4319 4704 4721 6173 Size: 60 63 21 1836 367 385 17 1452 Cuts at: 6173 6259 6271 6617 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1836 1452 385 367 346 86 63 60 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6070 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5883 Fragments arranged by size: 5883 683 56 BtsI GCAGTG_nn' Cuts at: 2817 3133 4138 4932 4952 2817 Size: 316 1005 794 20 4487 Fragments arranged by size: 4487 1005 794 316 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2328 2363 529 Size: 35 1764 35 4788 Fragments arranged by size: 4788 1764 35 35 DraI TTT'AAA Cuts at: 2881 3926 2881 Size: 1045 5577 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587 Size: 54 1425 50 275 18 2239 889 1629 Cuts at: 6587 8 Size: 43 Fragments arranged by size: 2239 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 2518 2853 2997 3159 3165 3278 4552 Size: 1035 130 335 144 162 6 113 1274 Cuts at: 4552 1353 Size: 3423 Fragments arranged by size: 3423 1274 1035 335 162 144 130 113 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2025 5209 5583 5729 387 Size: 1064 138 436 3184 374 146 1280 Fragments arranged by size: 3184 1280 1064 436 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 3087 3275 3476 3528 3903 4685 Size: 393 605 850 188 201 52 375 782 Cuts at: 4685 5282 6069 6337 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 850 787 782 605 597 393 375 268 201 188 52 EcoNI CCTnn'n_nnAGG Cuts at: 2850 2915 3257 2850 Size: 65 342 6215 Fragments arranged by size: 6215 342 65 EcoO109I rG'GnC_Cy Cuts at: 2587 2924 3667 3761 2587 Size: 337 743 94 5448 Fragments arranged by size: 5448 743 337 94 EcoRI G'AATT_C Cuts at: 3931 4309 3931 Size: 378 6244 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 6622 FspI TGC'GCA Cuts at: 5128 5128 Size: 6622 HaeII r_GCGC'y Cuts at: 1678 5769 1678 Size: 4091 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2432 2464 2830 Size: 32 135 32 508 32 320 32 366 Cuts at: 2830 2862 2941 2973 3200 3232 3460 3492 5263 Size: 32 79 32 227 32 228 32 1771 Cuts at: 5263 5295 5337 5369 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1771 508 366 320 228 227 135 79 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3844 3966 4212 669 Size: 988 281 1906 122 246 3079 Fragments arranged by size: 3079 1906 988 281 246 122 HindIII A'AGCT_T Cuts at: 748 3779 748 Size: 3031 3591 HpaI GTT'AAC Cuts at: 3844 4212 3844 Size: 368 6254 KpnI G_GTAC'C Cuts at: 3947 3947 Size: 6622 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2438 3054 5736 5920 1305 Size: 1133 616 2682 184 2007 Fragments arranged by size: 2682 2007 1133 616 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4800 Fragments arranged by size: 4800 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2775 3364 4580 4939 5098 518 Size: 648 1609 589 1216 359 159 2042 Fragments arranged by size: 2042 1609 1216 648 589 359 159 MspA1I CmG'CkG Cuts at: 1606 2322 2549 4038 4715 5863 6108 1606 Size: 716 227 1489 677 1148 245 2120 Fragments arranged by size: 2120 1489 1148 716 677 245 227 NaeI GCC'GGC Cuts at: 2750 2750 Size: 6622 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6070 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NgoMIV G'CCGG_C Cuts at: 2748 2748 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 2662 3980 5525 1132 Size: 97 1433 1318 1545 2229 Fragments arranged by size: 2229 1545 1433 1318 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6622 PciI A'CATG_T Cuts at: 2658 5521 2658 Size: 2863 3759 PflMI CCAn_nnn'nTGG Cuts at: 1108 3821 1108 Size: 2713 3909 PfoI T'CCnGG_A Cuts at: 2235 2235 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2924 3761 2587 Size: 337 837 5448 Fragments arranged by size: 5448 837 337 PshAI GACnn'nnGTC Cuts at: 1613 3545 3577 1613 Size: 1932 32 4658 Fragments arranged by size: 4658 1932 32 PsiI TTA'TAA Cuts at: 2402 4192 2402 Size: 1790 4832 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2783 2815 2783 Size: 32 6590 PstI C_TGCA'G Cuts at: 830 2580 3868 3974 830 Size: 1750 1288 106 3478 Fragments arranged by size: 3478 1750 1288 106 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 PvuII CAG'CTG Cuts at: 2322 2549 2322 Size: 227 6395 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SapI GCTCTTCn'nnn_ Cuts at: 2997 2997 Size: 6622 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SfcI C'TryA_G Cuts at: 826 1045 2576 3243 3864 3970 5105 5786 5977 Size: 219 1531 667 621 106 1135 681 191 Cuts at: 5977 826 Size: 1471 Fragments arranged by size: 1531 1471 1135 681 667 621 219 191 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3949 1872 Size: 2077 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4726 5627 5889 820 Size: 197 926 2783 901 262 1553 Fragments arranged by size: 2783 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3980 1132 Size: 2848 3774 SspI AAT'ATT Cuts at: 5 52 4546 5 Size: 47 4494 2081 Fragments arranged by size: 4494 2081 47 StuI AGG'CCT Cuts at: 1517 3888 1517 Size: 2371 4251 StyI C'CwwG_G Cuts at: 513 1065 3575 4061 513 Size: 552 2510 486 3074 Fragments arranged by size: 3074 2510 552 486 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3029 3797 4649 4666 4819 5004 5449 Size: 1255 1449 768 852 17 153 185 445 Cuts at: 5449 325 Size: 1498 Fragments arranged by size: 1498 1449 1255 852 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2969 4868 6580 Size: 274 80 33 51 1443 992 1899 1712 Cuts at: 6580 96 Size: 138 Fragments arranged by size: 1899 1712 1443 992 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6582 6614 6582 Size: 32 6590 Tth111I GACn'n_nGTC Cuts at: 1316 2603 1316 Size: 1287 5335 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2924 2924 Size: 6622 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6622 XmaI C'CCGG_G Cuts at: 1870 3947 1870 Size: 2077 4545 XmnI GAAnn'nnTTC Cuts at: 4751 4751 Size: 6622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI