Restriction map for
pFN21AB7878
(Linear) (Six-base) MAPSORT of: KIBB7878.seq Check: 7886 from: 1 to: 1917
KIBB7878.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 799 1917
Size: 799 1118
AccI GT'mk_AC
Cuts at: 0 1806 1917
Size: 1806 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1270 1917
Size: 1082 188 647
Fragments arranged by size:
1082 647 188
AflIII A'CryG_T
Cuts at: 0 653 1917
Size: 653 1264
AhdI GACnn_n'nnGTC
Cuts at: 0 441 1917
Size: 441 1476
AlwNI CAG_nnn'CTG
Cuts at: 0 1816 1917
Size: 1816 101
ApoI r'AATT_y
Cuts at: 0 1831 1917
Size: 1831 86
BamHI G'GATC_C
Cuts at: 0 27 111 1917
Size: 27 84 1806
Fragments arranged by size:
1806 84 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 652 1250 1463 1861 1917
Size: 293 359 598 213 398 56
Fragments arranged by size:
598 398 359 293 213 56
BbvCI CC'TCA_GC
Cuts at: 0 5 578 1917
Size: 5 573 1339
Fragments arranged by size:
1339 573 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 89 123 1917
Size: 89 34 1794
Fragments arranged by size:
1794 89 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 267 1892 1917
Size: 267 1625 25
Fragments arranged by size:
1625 267 25
BglII A'GATC_T
Cuts at: 0 90 1917
Size: 90 1827
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 724 1626 1917
Size: 270 454 902 291
Fragments arranged by size:
902 454 291 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1471 1523 1898 1917
Size: 232 1239 52 375 19
Fragments arranged by size:
1239 375 232 52 19
Bpu10I CC'TnA_GC
Cuts at: 0 5 578 1917
Size: 5 573 1339
Fragments arranged by size:
1339 573 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 1344 1917
Size: 1344 573
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1871 1901 1917
Size: 1871 30 16
Fragments arranged by size:
1871 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1087 1096 1162 1165 1474 1917
Size: 1087 9 66 3 309 443
Fragments arranged by size:
1087 443 309 66 9 3
BseYI C'CCAG_C
Cuts at: 0 1816 1917
Size: 1816 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1917
Size: 824 1093
BsiHKAI G_wGCw'C
Cuts at: 0 88 1004 1917
Size: 88 916 913
Fragments arranged by size:
916 913 88
Bsp1286I G_dGCh'C
Cuts at: 0 88 1004 1917
Size: 88 916 913
Fragments arranged by size:
916 913 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 799 1917
Size: 799 1118
BsrDI GCAATG_nn'
Cuts at: 0 1503 1917
Size: 1503 414
BsrFI r'CCGG_y
Cuts at: 0 743 1917
Size: 743 1174
BstBI TT'CG_AA
Cuts at: 0 51 1917
Size: 51 1866
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1361 1917
Size: 1361 556
BstYI r'GATC_y
Cuts at: 0 27 90 111 1917
Size: 27 63 21 1806
Fragments arranged by size:
1806 63 27 21
BtsI GCAGTG_nn'
Cuts at: 0 812 1128 1917
Size: 812 316 789
Fragments arranged by size:
812 789 316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 323 358 1917
Size: 323 35 1559
Fragments arranged by size:
1559 323 35
DraI TTT'AAA
Cuts at: 0 876 1917
Size: 876 1041
EarI CTCTTCn'nnn_
Cuts at: 0 383 513 848 992 1154 1160 1273 1917
Size: 383 130 335 144 162 6 113 644
Fragments arranged by size:
644 383 335 162 144 130 113 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 20 1917
Size: 20 1897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1082 1270 1471 1523 1898 1917
Size: 232 850 188 201 52 375 19
Fragments arranged by size:
850 375 232 201 188 52 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 845 910 1252 1917
Size: 845 65 342 665
Fragments arranged by size:
845 665 342 65
EcoO109I rG'GnC_Cy
Cuts at: 0 582 919 1662 1756 1917
Size: 582 337 743 94 161
Fragments arranged by size:
743 582 337 161 94
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 1917
Size: 693 1224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 427 459 825 857 936 968
Size: 75 32 320 32 366 32 79 32
Cuts at: 968 1195 1227 1455 1487 1917
Size: 227 32 228 32 430
Fragments arranged by size:
430 366 320 228 227 79 75 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1839 1917
Size: 1839 78
HindIII A'AGCT_T
Cuts at: 0 1774 1917
Size: 1774 143
HpaI GTT'AAC
Cuts at: 0 1839 1917
Size: 1839 78
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1049 1917
Size: 433 616 868
Fragments arranged by size:
868 616 433
MslI CAynn'nnrTG
Cuts at: 0 770 1359 1917
Size: 770 589 558
Fragments arranged by size:
770 589 558
MspA1I CmG'CkG
Cuts at: 0 317 544 1917
Size: 317 227 1373
Fragments arranged by size:
1373 317 227
NaeI GCC'GGC
Cuts at: 0 745 1917
Size: 745 1172
NgoMIV G'CCGG_C
Cuts at: 0 743 1917
Size: 743 1174
NspI r_CATG'y
Cuts at: 0 657 1917
Size: 657 1260
PciI A'CATG_T
Cuts at: 0 653 1917
Size: 653 1264
PflMI CCAn_nnn'nTGG
Cuts at: 0 1816 1917
Size: 1816 101
PfoI T'CCnGG_A
Cuts at: 0 230 1917
Size: 230 1687
PpuMI rG'GwC_Cy
Cuts at: 0 582 919 1756 1917
Size: 582 337 837 161
Fragments arranged by size:
837 582 337 161
PshAI GACnn'nnGTC
Cuts at: 0 1540 1572 1917
Size: 1540 32 345
Fragments arranged by size:
1540 345 32
PsiI TTA'TAA
Cuts at: 0 397 1917
Size: 397 1520
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 778 810 1917
Size: 778 32 1107
Fragments arranged by size:
1107 778 32
PstI C_TGCA'G
Cuts at: 0 575 1863 1917
Size: 575 1288 54
Fragments arranged by size:
1288 575 54
PvuII CAG'CTG
Cuts at: 0 317 544 1917
Size: 317 227 1373
Fragments arranged by size:
1373 317 227
SapI GCTCTTCn'nnn_
Cuts at: 0 992 1917
Size: 992 925
SfcI C'TryA_G
Cuts at: 0 571 1238 1859 1917
Size: 571 667 621 58
Fragments arranged by size:
667 621 571 58
StuI AGG'CCT
Cuts at: 0 1883 1917
Size: 1883 34
StyI C'CwwG_G
Cuts at: 0 1570 1917
Size: 1570 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1024 1792 1917
Size: 1024 768 125
Fragments arranged by size:
1024 768 125
TatI w'GTAC_w
Cuts at: 0 964 1917
Size: 964 953
Tth111I GACn'n_nGTC
Cuts at: 0 598 1917
Size: 598 1319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 919 1917
Size: 919 998
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApoI BamHI
BbsI BbvCI BcgI BciVI BglII BmrI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BstBI BstXI BstYI BtsI CspCI DraI
EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
HindIII HpaI MmeI MslI MspA1I NaeI NgoMIV NspI
PciI PflMI PfoI PpuMI PshAI PsiI PsrI PstI
PvuII SapI SfcI StuI StyI TaqII TatI Tth111I
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BanII BbeI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI DraIII DrdI EaeI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmaI XmnI ZraI