(Circular) (Six-base) MAPSORT of: pFN21AB7763.seq Check: 7163 from: 1 to: 6049 pFN21AB7763 6049 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2174 2174 Size: 6049 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2214 3392 1937 Size: 277 1178 4594 Fragments arranged by size: 4594 1178 277 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 2075 4176 4549 1563 Size: 512 2101 373 3063 Fragments arranged by size: 3063 2101 512 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 4112 5496 5764 1632 Size: 701 1779 1384 268 1917 Fragments arranged by size: 1917 1779 1384 701 268 AflII C'TTAA_G Cuts at: 820 1017 3164 820 Size: 197 2147 3705 Fragments arranged by size: 3705 2147 197 AflIII A'CryG_T Cuts at: 1203 4948 5925 1203 Size: 3745 977 1327 Fragments arranged by size: 3745 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 2672 2828 1166 Size: 1506 156 4387 Fragments arranged by size: 4387 1506 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2253 1184 Size: 1069 4980 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2910 2942 2910 Size: 32 6017 AlwNI CAG_nnn'CTG Cuts at: 3792 5364 3792 Size: 1572 4477 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 2408 2659 2767 3358 3584 3736 5845 1493 Size: 915 251 108 591 226 152 2109 1697 Fragments arranged by size: 2109 1697 915 591 251 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 3374 1870 Size: 73 1431 4545 Fragments arranged by size: 4545 1431 73 BamHI G'GATC_C Cuts at: 3379 3746 3379 Size: 367 5682 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 3370 4825 618 Size: 325 217 627 303 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 3109 3368 1391 Size: 1718 259 4072 Fragments arranged by size: 4072 1718 259 BbeI G_GCGC'C Cuts at: 2094 2094 Size: 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3032 928 Size: 971 1133 3945 Fragments arranged by size: 3945 1133 971 BbvCI CC'TCA_GC Cuts at: 2700 3231 2700 Size: 531 5518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2234 2268 4240 4274 1903 Size: 34 297 34 1972 34 3678 Fragments arranged by size: 3678 1972 297 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4660 136 Size: 107 122 71 631 605 2988 1525 Fragments arranged by size: 2988 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 4110 5266 1790 Size: 2320 1156 2573 Fragments arranged by size: 2573 2320 1156 BmgBI CAC'GTC Cuts at: 1318 2565 1318 Size: 1247 4802 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3300 3780 4738 Size: 416 53 83 399 24 1883 480 958 Cuts at: 4738 442 Size: 1753 Fragments arranged by size: 1883 1753 958 480 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 2459 2898 3071 3191 4709 1239 Size: 1217 3 439 173 120 1518 2579 Fragments arranged by size: 2579 1518 1217 439 173 120 3 Bpu10I CC'TnA_GC Cuts at: 2700 3231 2700 Size: 531 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4174 5039 5337 4174 Size: 865 298 4886 Fragments arranged by size: 4886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4712 882 Size: 617 3213 2219 Fragments arranged by size: 3213 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3840 493 Size: 711 2636 2702 Fragments arranged by size: 2702 2636 711 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 3856 4238 4873 Size: 53 83 186 936 558 1765 382 635 Cuts at: 4873 275 Size: 1451 Fragments arranged by size: 1765 1451 936 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2376 2406 3111 3141 1178 Size: 30 165 30 973 30 705 30 4086 Fragments arranged by size: 4086 973 705 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2972 1186 Size: 1786 4263 BseYI C'CCAG_C Cuts at: 5252 5748 5252 Size: 496 5553 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2307 1163 Size: 573 571 4905 Fragments arranged by size: 4905 573 571 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4110 4195 5266 4110 Size: 85 1071 4893 Fragments arranged by size: 4893 1071 85 BsmI GAATG_Cn' Cuts at: 2302 3560 3653 2302 Size: 1258 93 4698 Fragments arranged by size: 4698 1258 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3109 3368 4110 4195 5266 1391 Size: 399 1319 259 742 85 1071 2174 Fragments arranged by size: 2174 1319 1071 742 399 259 85 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 2947 3404 844 Size: 1330 773 457 3489 Fragments arranged by size: 3489 1330 773 457 BsrBI CCG'CTC Cuts at: 1394 1823 3936 1394 Size: 429 2113 3507 Fragments arranged by size: 3507 2113 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 3064 4544 4718 65 Size: 1183 1239 577 1480 174 1396 Fragments arranged by size: 1480 1396 1239 1183 577 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 2696 794 Size: 1902 4147 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 2627 1184 Size: 1443 4606 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6049 BstYI r'GATC_y Cuts at: 1972 3379 3746 4131 4148 5600 5686 5698 6044 Size: 1407 367 385 17 1452 86 12 346 Cuts at: 6044 1972 Size: 1977 Fragments arranged by size: 1977 1452 1407 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2623 2623 Size: 6049 BtgI C'CryG_G Cuts at: 513 1065 3213 3330 513 Size: 552 2148 117 3232 Fragments arranged by size: 3232 2148 552 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 3565 4359 4379 3565 Size: 794 20 5235 Fragments arranged by size: 5235 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3018 3053 529 Size: 35 2454 35 3525 Fragments arranged by size: 3525 2454 35 35 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2263 2263 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2887 3496 4385 Size: 54 1425 50 275 18 1057 609 889 Cuts at: 4385 6014 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1057 889 609 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2979 3097 3216 3264 3979 1353 Size: 1626 118 119 48 715 3423 Fragments arranged by size: 3423 1626 715 119 118 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2456 2459 2898 3071 3191 4112 Size: 393 701 123 3 439 173 120 921 Cuts at: 4112 4709 5496 5764 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 921 787 701 597 439 393 268 173 123 120 3 EcoNI CCTnn'n_nnAGG Cuts at: 2877 2877 Size: 6049 EcoO109I rG'GnC_Cy Cuts at: 2220 2220 Size: 6049 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2192 2192 Size: 6049 FspI TGC'GCA Cuts at: 2256 4555 2256 Size: 2299 3750 FspAI rTGC'GCAy Cuts at: 2256 2256 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2094 2347 5196 1678 Size: 416 253 2849 2531 Fragments arranged by size: 2849 2531 416 253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 3111 3143 4690 Size: 32 135 32 804 32 703 32 1547 Cuts at: 4690 4722 4764 4796 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1547 804 703 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2581 2776 3393 3639 669 Size: 988 281 643 195 617 246 3079 Fragments arranged by size: 3079 988 643 617 281 246 195 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 2581 3639 2581 Size: 1058 4991 KasI G'GCGC_C Cuts at: 2090 2090 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2745 3246 5163 5347 1305 Size: 1440 501 1917 184 2007 Fragments arranged by size: 2007 1917 1440 501 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2889 10 Size: 54 1425 343 1057 3170 Fragments arranged by size: 3170 1425 1057 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 2672 2828 4007 4366 4525 518 Size: 648 1189 317 156 1179 359 159 2042 Fragments arranged by size: 2042 1189 1179 648 359 317 159 156 MspA1I CmG'CkG Cuts at: 1606 3465 4142 5290 5535 1606 Size: 1859 677 1148 245 2120 Fragments arranged by size: 2120 1859 1148 677 245 NarI GG'CG_CC Cuts at: 2091 2091 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 3213 3330 513 Size: 552 2148 117 3232 Fragments arranged by size: 3232 2148 552 117 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2255 2311 3407 4952 1132 Size: 97 1026 56 1096 1545 2229 Fragments arranged by size: 2229 1545 1096 1026 97 56 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2109 1108 Size: 1001 5048 PfoI T'CCnGG_A Cuts at: 2472 3087 2472 Size: 615 5434 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 2220 Size: 6049 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PstI C_TGCA'G Cuts at: 830 2242 3401 830 Size: 1412 1159 3478 Fragments arranged by size: 3478 1412 1159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SapI GCTCTTCn'nnn_ Cuts at: 3097 3097 Size: 6049 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 2172 4297 6009 2172 Size: 2125 1712 2212 Fragments arranged by size: 2212 2125 1712 SfcI C'TryA_G Cuts at: 826 1045 2238 3074 3243 3397 4532 5213 5404 Size: 219 1193 836 169 154 1135 681 191 Cuts at: 5404 826 Size: 1471 Fragments arranged by size: 1471 1193 1135 836 681 219 191 169 154 SfoI GGC'GCC Cuts at: 2092 2092 Size: 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3164 4153 5054 5316 820 Size: 197 926 1221 989 901 262 1553 Fragments arranged by size: 1553 1221 989 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3407 1132 Size: 2275 3774 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 2301 3011 3213 3330 3488 513 Size: 552 1236 710 202 117 158 3074 Fragments arranged by size: 3074 1236 710 552 202 158 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4076 4093 4246 4431 4876 325 Size: 1255 2496 17 153 185 445 1498 Fragments arranged by size: 2496 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2170 2647 4295 Size: 274 80 33 51 1443 193 477 1648 Cuts at: 4295 6007 96 Size: 1712 138 Fragments arranged by size: 1712 1648 1443 477 274 193 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6009 6041 6009 Size: 32 6017 Tth111I GACn'n_nGTC Cuts at: 1316 3292 1316 Size: 1976 4073 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2674 2011 Size: 663 5386 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI