(Circular) (Six-base) MAPSORT of: pFN21AB8991.seq Check: 4661 from: 1 to: 5170 pFN21AB8991 5170 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2980 278 Size: 53 83 186 936 1444 2468 Fragments arranged by size: 2468 1444 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2513 1937 Size: 576 4594 Acc65I G'GTAC_C Cuts at: 2491 2491 Size: 5170 AclI AA'CG_TT Cuts at: 1563 3297 3670 1563 Size: 1734 373 3063 Fragments arranged by size: 3063 1734 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3233 4617 4885 1632 Size: 1601 1384 268 1917 Fragments arranged by size: 1917 1601 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4973 AflIII A'CryG_T Cuts at: 1203 4069 5046 1203 Size: 2866 977 1327 Fragments arranged by size: 2866 1327 977 AgeI A'CCGG_T Cuts at: 4001 4001 Size: 5170 AhdI GACnn_n'nnGTC Cuts at: 3899 3899 Size: 5170 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5170 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 2069 Size: 32 5138 AlwNI CAG_nnn'CTG Cuts at: 2913 4485 2913 Size: 1572 3598 ApaI G_GGCC'C Cuts at: 2012 2012 Size: 5170 ApaLI G'TGCA_C Cuts at: 3227 4383 3227 Size: 1156 4014 ApoI r'AATT_y Cuts at: 1493 2040 2479 2705 2857 4966 1493 Size: 547 439 226 152 2109 1697 Fragments arranged by size: 2109 1697 547 439 226 152 AseI AT'TA_AT Cuts at: 160 1032 3724 160 Size: 872 2692 1606 Fragments arranged by size: 2692 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5170 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2230 2495 1870 Size: 73 69 218 265 4545 Fragments arranged by size: 4545 265 218 73 69 BamHI G'GATC_C Cuts at: 2500 2867 2500 Size: 367 4803 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2491 3946 618 Size: 325 217 627 704 1455 1842 Fragments arranged by size: 1842 1455 704 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2055 2489 1391 Size: 621 43 434 4072 Fragments arranged by size: 4072 621 434 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2221 2255 3361 3395 1903 Size: 34 284 34 1106 34 3678 Fragments arranged by size: 3678 1106 284 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3062 4272 3062 Size: 1210 3960 BclI T'GATC_A Cuts at: 1548 1548 Size: 5170 BfrBI ATG'CAT Cuts at: 2338 2338 Size: 5170 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2306 3781 136 Size: 107 122 71 631 605 634 1475 1525 Fragments arranged by size: 1525 1475 634 631 605 122 107 71 BglII A'GATC_T Cuts at: 5165 5165 Size: 5170 BlpI GC'TnA_GC Cuts at: 2587 2587 Size: 5170 Bme1580I G_kGCm'C Cuts at: 1790 2012 3231 4387 1790 Size: 222 1219 1156 2573 Fragments arranged by size: 2573 1219 1156 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5170 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2901 3859 442 Size: 416 53 83 399 24 1484 958 1753 Fragments arranged by size: 1753 1484 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5170 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2192 3830 1239 Size: 800 153 1638 2579 Fragments arranged by size: 2579 1638 800 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3295 4160 4458 3295 Size: 865 298 4007 Fragments arranged by size: 4007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3833 882 Size: 617 2334 2219 Fragments arranged by size: 2334 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2961 493 Size: 711 1757 2702 Fragments arranged by size: 2702 1757 711 BsaBI GATnn'nnATC Cuts at: 5164 5164 Size: 5170 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2977 3359 3994 275 Size: 53 83 186 936 1444 382 635 1451 Fragments arranged by size: 1451 1444 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2464 3603 4001 4275 4422 4833 2464 Size: 1139 398 274 147 411 2801 Fragments arranged by size: 2801 1139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 1178 Size: 30 165 30 666 30 4249 Fragments arranged by size: 4249 666 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2288 1186 Size: 973 129 4068 Fragments arranged by size: 4068 973 129 BseYI C'CCAG_C Cuts at: 4373 4869 4373 Size: 496 4674 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2261 2411 1163 Size: 573 501 24 150 3922 Fragments arranged by size: 3922 573 501 150 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2620 3381 4409 1540 Size: 275 186 619 761 1028 2301 Fragments arranged by size: 2301 1028 761 619 275 186 BsiHKAI G_wGCw'C Cuts at: 2132 3231 3316 4387 2132 Size: 1099 85 1071 2915 Fragments arranged by size: 2915 1099 1071 85 BsmI GAATG_Cn' Cuts at: 2681 2774 2681 Size: 93 5077 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2055 2132 2489 3231 3316 4387 Size: 399 222 43 77 357 742 85 1071 Cuts at: 4387 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 357 222 85 77 43 BspEI T'CCGG_A Cuts at: 2464 4833 2464 Size: 2369 2801 BspHI T'CATG_A Cuts at: 3059 4789 3059 Size: 1730 3440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2525 844 Size: 1681 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2075 3057 1394 Size: 429 252 982 3507 Fragments arranged by size: 3507 982 429 252 BsrDI GCAATG_nn' Cuts at: 65 1248 3665 3839 65 Size: 1183 2417 174 1396 Fragments arranged by size: 2417 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3814 4001 1700 Size: 110 2004 187 2869 Fragments arranged by size: 2869 2004 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5170 BssHII G'CGCG_C Cuts at: 1925 2197 2221 1925 Size: 272 24 4874 Fragments arranged by size: 4874 272 24 BssSI C'ACGA_G Cuts at: 3230 4242 3230 Size: 1012 4158 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5170 BstBI TT'CG_AA Cuts at: 3974 3974 Size: 5170 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5170 BstYI r'GATC_y Cuts at: 1972 2500 2867 3252 3269 4721 4807 4819 5165 Size: 528 367 385 17 1452 86 12 346 Cuts at: 5165 1972 Size: 1977 Fragments arranged by size: 1977 1452 528 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3679 Fragments arranged by size: 3679 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4431 Fragments arranged by size: 4431 683 56 BtsI GCAGTG_nn' Cuts at: 2686 3480 3500 2686 Size: 794 20 4356 Fragments arranged by size: 4356 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5170 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5135 DraI TTT'AAA Cuts at: 2474 2474 Size: 5170 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4177 809 Size: 507 2861 1802 Fragments arranged by size: 2861 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 2617 3506 Size: 54 1425 50 275 18 477 310 889 Cuts at: 3506 5135 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 477 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2617 1537 Size: 275 805 4090 Fragments arranged by size: 4090 805 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3100 1353 Size: 1747 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2012 2336 3757 4131 4277 387 Size: 1064 138 423 324 1421 374 146 1280 Fragments arranged by size: 1421 1280 1064 423 374 324 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2192 3233 3830 4617 4885 1239 Size: 393 407 153 1041 597 787 268 1524 Fragments arranged by size: 1524 1041 787 597 407 393 268 153 EcoO109I rG'GnC_Cy Cuts at: 2009 2149 2176 2009 Size: 140 27 5003 Fragments arranged by size: 5003 140 27 EcoRI G'AATT_C Cuts at: 2479 2857 2479 Size: 378 4792 FspI TGC'GCA Cuts at: 3676 3676 Size: 5170 HaeII r_GCGC'y Cuts at: 1678 4317 1678 Size: 2639 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3811 3843 3885 3917 1373 Size: 32 135 32 2239 32 42 32 2626 Fragments arranged by size: 2626 2239 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2514 2760 669 Size: 988 281 576 246 3079 Fragments arranged by size: 3079 988 576 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5170 HpaI GTT'AAC Cuts at: 2760 2760 Size: 5170 KpnI G_GTAC'C Cuts at: 2495 2495 Size: 5170 MfeI C'AATT_G Cuts at: 2396 2769 2396 Size: 373 4797 MluI A'CGCG_T Cuts at: 5046 5046 Size: 5170 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4284 4468 1305 Size: 2979 184 2007 Fragments arranged by size: 2979 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 10 Size: 54 1425 343 477 2871 Fragments arranged by size: 2871 1425 477 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 3128 3487 3646 518 Size: 648 1221 741 359 159 2042 Fragments arranged by size: 2042 1221 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2167 2356 2586 3263 4411 4656 1606 Size: 422 139 189 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 422 245 230 189 139 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3679 Fragments arranged by size: 3679 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5170 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5170 NotI GC'GGCC_GC Cuts at: 2617 2617 Size: 5170 NsiI A_TGCA'T Cuts at: 2340 2340 Size: 5170 NspI r_CATG'y Cuts at: 1132 1229 2528 4073 1132 Size: 97 1299 1545 2229 Fragments arranged by size: 2229 1545 1299 97 PasI CC'CwG_GG Cuts at: 1348 2047 1348 Size: 699 4471 PciI A'CATG_T Cuts at: 4069 4069 Size: 5170 PflMI CCAn_nnn'nTGG Cuts at: 1108 2356 1108 Size: 1248 3922 PfoI T'CCnGG_A Cuts at: 2139 2139 Size: 5170 PmeI GTTT'AAAC Cuts at: 2474 2474 Size: 5170 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 2101 3239 3271 4752 4784 2069 Size: 32 1138 32 1481 32 2455 Fragments arranged by size: 2455 1481 1138 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2176 Size: 5170 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5170 PsiI TTA'TAA Cuts at: 2740 2740 Size: 5170 PspOMI G'GGCC_C Cuts at: 2008 2008 Size: 5170 PstI C_TGCA'G Cuts at: 830 2033 2522 830 Size: 1203 489 3478 Fragments arranged by size: 3478 1203 489 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5170 PvuII CAG'CTG Cuts at: 2356 2356 Size: 5170 SalI G'TCGA_C Cuts at: 1936 2512 1936 Size: 576 4594 SbfI CC_TGCA'GG Cuts at: 2522 2522 Size: 5170 ScaI AGT'ACT Cuts at: 3418 5130 3418 Size: 1712 3458 SfcI C'TryA_G Cuts at: 826 1045 2029 2518 3653 4334 4525 826 Size: 219 984 489 1135 681 191 1471 Fragments arranged by size: 1471 1135 984 681 489 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2306 2306 Size: 5170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5170 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5170 SmaI CCC'GGG Cuts at: 1872 2014 2497 1872 Size: 142 483 4545 Fragments arranged by size: 4545 483 142 SmlI C'TyrA_G Cuts at: 820 1017 1943 2230 3274 4175 4437 820 Size: 197 926 287 1044 901 262 1553 Fragments arranged by size: 1553 1044 926 901 287 262 197 SnaBI TAC'GTA Cuts at: 493 2961 493 Size: 2468 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5170 SphI G_CATG'C Cuts at: 1132 2528 1132 Size: 1396 3774 SspI AAT'ATT Cuts at: 5 52 3094 5 Size: 47 3042 2081 Fragments arranged by size: 3042 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5170 StyI C'CwwG_G Cuts at: 513 1065 2004 2609 513 Size: 552 939 605 3074 Fragments arranged by size: 3074 939 605 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3197 3214 3367 3552 3997 325 Size: 1255 1617 17 153 185 445 1498 Fragments arranged by size: 1617 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3416 5128 96 Size: 274 80 33 51 1443 1439 1712 138 Fragments arranged by size: 1712 1443 1439 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5130 5162 5130 Size: 32 5138 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5170 XbaI T'CTAG_A Cuts at: 2506 2506 Size: 5170 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2167 Size: 5170 XhoI C'TCGA_G Cuts at: 1943 2230 1943 Size: 287 4883 XmaI C'CCGG_G Cuts at: 1870 2012 2495 1870 Size: 142 483 4545 Fragments arranged by size: 4545 483 142 XmnI GAAnn'nnTTC Cuts at: 3299 3299 Size: 5170 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2978 276 Size: 53 83 186 936 1444 2468 Fragments arranged by size: 2468 1444 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI