Restriction map for
pFN21AB7224
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Alw44I 308 GTGCAC G^TGCAC
AocI 753 CCTGAGG CC^T[ACGT]AGG
ApaLI 308 GTGCAC G^TGCAC
AvaI 566 CCCGGG C^[CT]CG[GA]G
AviII 274 TGCGCA TGC^GCA
BalI 162 TGGCCA TGG^CCA
BclI 737 TGATCA T^GATCA
BglI 505 GCCGTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 630 CTCCAG CTGGAG(16/14)
BsaI 4 GGTCTC GGTCTC(1/5)
BsgI 388 CTGCAC GTGCAG(16/14)
BsoBI 566 CCCGGG C^[CT]CG[GA]G
BssSI 727 CTCGTG CACGAG(-5/-1)
BstEII 24 GGTCACC G^GT[ACGT]ACC
BstPI 24 GGTCACC G^GT[ACGT]ACC
BstXI 446 CCACAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1005 AGATCC [GA]^GATC[CT]
Bsu36I 753 CCTGAGG CC^T[ACGT]AGG
DrdI 249 GACTCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 162 TGGCCA [CT]^GGCC[GA]
EarI 595 GAAGAG CTCTTC(1/4)
Eco81I 753 CCTGAGG CC^T[ACGT]AGG
EcoO65I 24 GGTCACC G^GT[ACGT]ACC
FauI 147 GCGGG CCCGC(4/6)
FbaI 737 TGATCA T^GATCA
FspI 274 TGCGCA TGC^GCA
Ksp632I 595 GAAGAG CTCTTC(1/4)
MaeII 829 ACGT A^CGT
MboII 595 GAAGA GAAGA(8/7)
MflI 1005 AGATCC [GA]^GATC[CT]
MluNI 162 TGGCCA TGG^CCA
MscI 162 TGGCCA TGG^CCA
PflMI 666 CCATACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 249 GACTC GAGTC(4/5)
PstI 30 CTGCAG CTGCA^G
PvuII 717 CAGCTG CAG^CTG
SbfI 29 CCTGCAGG CCTGCA^GG
SfaNI 73 GCATC GCATC(5/9)
SmaI 566 CCCGGG CCC^GGG
Sse8387I 29 CCTGCAGG CCTGCA^GG
TaqI 354 TCGA T^CGA
TthHB8I 354 TCGA T^CGA
Van91I 666 CCATACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 323 CCAAAGGCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1005 AGATCC [GA]^GATC[CT]
XmaCI 566 CCCGGG C^CCGGG
XmaI 566 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 146 CGCG CG^CG
916 CGCG
AcyI 136 GACGCC G[GA]^CG[CT]C
229 GACGCC
AlwI 1006 GATCC GGATC(4/5)
570 GGATC
ApaI 347 GGGCCC GGGCC^C
575 GGGCCC
AspHI 308 GTGCAC G[AT]GC[AT]^C
458 GTGCTC
BsaHI 136 GACGCC G[GA]^CG[CT]C
229 GACGCC
BsiHKAI 308 GTGCAC G[AT]GC[AT]^C
458 GTGCTC
BsmAI 786 GAGAC GTCTC(1/5)
5 GTCTC
BstAPI 266 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1029 GCACTGTATGC
BstUI 146 CGCG CG^CG
916 CGCG
DsaI 332 CCATGG C^C[GA][CT]GG
671 CCATGG
EcoNI 364 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
753 CCTGAGGGAGG
HaeII 513 GGCGCT [GA]GCGC^[CT]
732 GGCGCT
Hin1I 136 GACGCC G[GA]^CG[CT]C
229 GACGCC
MslI 393 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CACAACAGTG
MvnI 146 CGCG CG^CG
916 CGCG
NcoI 332 CCATGG C^CATGG
671 CCATGG
PpuMI 34 AGGTCCC [GA]G^G[AT]CC[CT]
466 AGGTCCC
SfcI 30 CTGCAG C^T[GA][CT]AG
472 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaII BanII BbvCI
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI StyI TfiI
TseI Tsp45I TspRI