(Circular) (Six-base) MAPSORT of: pFN21AB7224.seq Check: 5570 from: 1 to: 5773 pFN21AB7224 5773 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2042 2179 2042 Size: 137 5636 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2358 2898 3836 5220 5488 1632 Size: 726 540 938 1384 268 1917 Fragments arranged by size: 1917 1384 938 726 540 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 2456 1166 Size: 1290 4483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaI G_GGCC'C Cuts at: 2356 2584 2356 Size: 228 5545 ApaLI G'TGCA_C Cuts at: 2313 3830 4986 2313 Size: 1517 1156 3100 Fragments arranged by size: 3100 1517 1156 ApoI r'AATT_y Cuts at: 1493 3082 3308 3460 5569 1493 Size: 1589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1589 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 2571 3098 1870 Size: 73 628 527 4545 Fragments arranged by size: 4545 628 527 73 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3094 4549 618 Size: 325 217 627 1307 1455 1842 Fragments arranged by size: 1842 1455 1307 627 325 217 BanII G_rGCy'C Cuts at: 1391 2356 2442 2584 3092 1391 Size: 965 86 142 508 4072 Fragments arranged by size: 4072 965 508 142 86 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4802 BbvCI CC'TCA_GC Cuts at: 2198 2522 2720 2870 2198 Size: 324 198 150 5101 Fragments arranged by size: 5101 324 198 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 2742 1548 Size: 1194 4579 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2516 4384 136 Size: 107 122 71 631 605 844 1868 1525 Fragments arranged by size: 1868 1525 844 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2317 2356 2584 2904 3834 4990 1790 Size: 527 39 228 320 930 1156 2573 Fragments arranged by size: 2573 1156 930 527 320 228 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2650 2650 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2620 4433 1239 Size: 1381 1813 2579 Fragments arranged by size: 2579 1813 1381 Bpu10I CC'TnA_GC Cuts at: 2198 2522 2720 2870 2198 Size: 324 198 150 5101 Fragments arranged by size: 5101 324 198 150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 4436 882 Size: 617 516 2421 2219 Fragments arranged by size: 2421 2219 617 516 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2235 3580 3962 Size: 53 83 186 936 609 93 1345 382 Cuts at: 3962 4597 275 Size: 635 1451 Fragments arranged by size: 1451 1345 936 635 609 382 186 93 83 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2089 2119 2404 2434 1178 Size: 30 165 30 686 30 285 30 4517 Fragments arranged by size: 4517 686 285 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2436 2780 2952 1186 Size: 1250 344 172 4007 Fragments arranged by size: 4007 1250 344 172 BseYI C'CCAG_C Cuts at: 2056 2535 4976 5472 2056 Size: 479 2441 496 2357 Fragments arranged by size: 2441 2357 496 479 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 1163 Size: 573 642 4558 Fragments arranged by size: 4558 642 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2317 2467 3834 3919 4990 2317 Size: 150 1367 85 1071 3100 Fragments arranged by size: 3100 1367 1071 150 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 2356 2442 2467 2584 2904 3092 Size: 399 527 39 86 25 117 320 188 Cuts at: 3092 3834 3919 4990 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 527 399 320 188 117 86 85 39 25 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 2042 2179 3128 844 Size: 1184 14 137 949 3489 Fragments arranged by size: 3489 1184 949 137 14 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4417 4604 1700 Size: 110 2607 187 2869 Fragments arranged by size: 2869 2607 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 2732 3833 4845 2732 Size: 1101 1012 3660 Fragments arranged by size: 3660 1101 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2277 3040 794 Size: 1483 763 3527 Fragments arranged by size: 3527 1483 763 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 2029 1184 Size: 845 4928 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2458 Size: 5773 BstYI r'GATC_y Cuts at: 1972 3010 3103 3470 3855 3872 5324 5410 5422 Size: 1038 93 367 385 17 1452 86 12 Cuts at: 5422 5768 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1038 385 367 346 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2759 2759 Size: 5773 BtgI C'CryG_G Cuts at: 513 1065 2004 2337 2676 513 Size: 552 939 333 339 3610 Fragments arranged by size: 3610 939 552 339 333 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3289 4083 4103 2503 Size: 786 794 20 4173 Fragments arranged by size: 4173 794 786 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2260 4780 809 Size: 507 944 2520 1802 Fragments arranged by size: 2520 1802 944 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 3220 4109 Size: 54 1425 50 275 18 337 1053 889 Cuts at: 4109 5738 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1053 889 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2595 3703 1353 Size: 1242 1108 3423 Fragments arranged by size: 3423 1242 1108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2792 2945 4360 4734 4880 387 Size: 1064 138 1203 153 1415 374 146 1280 Fragments arranged by size: 1415 1280 1203 1064 374 153 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2358 2620 2898 3836 4433 5220 5488 Size: 393 726 262 278 938 597 787 268 Cuts at: 5488 1239 Size: 1524 Fragments arranged by size: 1524 938 787 726 597 393 278 268 262 EcoNI CCTnn'n_nnAGG Cuts at: 2373 2762 2373 Size: 389 5384 EcoO109I rG'GnC_Cy Cuts at: 2040 2352 2353 2472 2040 Size: 312 1 119 5341 Fragments arranged by size: 5341 312 119 1 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FspI TGC'GCA Cuts at: 2281 4279 2281 Size: 1998 3775 HaeII r_GCGC'y Cuts at: 1678 2522 2741 4920 1678 Size: 844 219 2179 2531 Fragments arranged by size: 2531 2179 844 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2404 2436 2814 Size: 32 135 32 614 32 186 32 378 Cuts at: 2814 2846 4414 4446 4488 4520 1373 Size: 32 1568 32 42 32 2626 Fragments arranged by size: 2626 1568 614 378 186 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3027 4887 5071 1305 Size: 1722 1860 184 2007 Fragments arranged by size: 2007 1860 1722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 10 Size: 54 1425 343 337 3614 Fragments arranged by size: 3614 1425 343 337 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2456 3731 4090 4249 518 Size: 648 1236 54 1275 359 159 2042 Fragments arranged by size: 2042 1275 1236 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2658 2695 2724 3189 3866 5014 5259 1606 Size: 1052 37 29 465 677 1148 245 2120 Fragments arranged by size: 2120 1148 1052 677 465 245 37 29 NcoI C'CATG_G Cuts at: 513 1065 2004 2337 2676 513 Size: 552 939 333 339 3610 Fragments arranged by size: 3610 939 552 339 333 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 2387 1348 Size: 1039 4734 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 2677 1108 Size: 1569 4204 PfoI T'CCnGG_A Cuts at: 2229 2229 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 2472 2040 Size: 432 5341 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5773 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PspOMI G'GGCC_C Cuts at: 2352 2580 2352 Size: 228 5545 PstI C_TGCA'G Cuts at: 830 2039 3125 830 Size: 1209 1086 3478 Fragments arranged by size: 3478 1209 1086 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 2724 2724 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 2039 3125 2039 Size: 1086 4687 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2035 2477 3121 4256 4937 5128 826 Size: 219 990 442 644 1135 681 191 1471 Fragments arranged by size: 1471 1135 990 681 644 442 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 2573 3100 1872 Size: 701 527 4545 Fragments arranged by size: 4545 701 527 SmlI C'TyrA_G Cuts at: 820 1017 1943 3877 4778 5040 820 Size: 197 926 1934 901 262 1553 Fragments arranged by size: 1934 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 2004 2238 2337 2676 2983 3014 3212 Size: 552 939 234 99 339 307 31 198 Cuts at: 3212 513 Size: 3074 Fragments arranged by size: 3074 939 552 339 307 234 198 99 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 3800 3817 3970 4155 4600 325 Size: 1255 870 1350 17 153 185 445 1498 Fragments arranged by size: 1498 1350 1255 870 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4019 5731 96 Size: 274 80 33 51 1443 2042 1712 138 Fragments arranged by size: 2042 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2335 2011 Size: 324 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 2571 3098 1870 Size: 701 527 4545 Fragments arranged by size: 4545 701 527 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI