Restriction map for
pFN21AB7383
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BanI
BanII BbeI BbrPI BbvI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpmI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI TseI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 281 GTAGAC GT^[AC][GT]AC
AcsI 157 AAATTT [GA]^AATT[CT]
AfaI 250 GTAC GT^AC
ApoI 157 AAATTT [GA]^AATT[CT]
Asp700I 547 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 466 GAGCAC G[AT]GC[AT]^C
AvaII 269 GGTCC G^G[AT]CC
AviII 561 TGCGCA TGC^GCA
BamHI 238 GGATCC G^GATCC
BcnI 639 CCGGG CC^[GC]GG
BglI 684 GCCCTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 27 GCTGAGC GC^T[ACGT]AGC
BmyI 466 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 27 GCTGAGC GC^T[ACGT]AGC
BseRI 743 CTCCTC GAGGAG(10/8)
BsgI 174 CTGCAC GTGCAG(16/14)
BsiHKAI 466 GAGCAC G[AT]GC[AT]^C
BsiYI 279 CCGTAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 279 CCGTAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 218 CGTCTC CGTCTC(1/5)
BsmFI 97 GGGAC GGGAC(10/14)
Bsp1286I 466 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 249 TGTACA T^GTACA
BspHI 396 TCATGA T^CATGA
BsrBI 30 GAGCGG CCGCTC(-3/-3)
BsrGI 249 TGTACA T^GTACA
BsrI 689 ACTGG ACTGG(1/-1)
BstAPI 564 GCAAGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 238 GGATCC [GA]^GATC[CT]
CelII 27 GCTGAGC GC^T[ACGT]AGC
CfoI 562 GCGC GCG^C
DpnII 239 GATC ^GATC
DraI 714 TTTAAA TTT^AAA
DsaI 242 CCACGG C^C[GA][CT]GG
EcoT14I 118 CCTTGG C^C[AT][AT]GG
FauI 579 GCGGG CCCGC(4/6)
FspI 561 TGCGCA TGC^GCA
HapII 639 CCGG C^CGG
HhaI 562 GCGC GCG^C
HinP1I 562 GCGC G^CGC
HincII 388 GTCAAC GT[CT]^[GA]AC
HindII 388 GTCAAC GT[CT]^[GA]AC
HpaII 639 CCGG C^CGG
MboI 239 GATC ^GATC
MflI 238 GGATCC [GA]^GATC[CT]
MspI 639 CCGG C^CGG
NciI 639 CCGGG CC^[GC]GG
NdeII 239 GATC ^GATC
NlaIV 238 GGATCC GG[ACGT]^[ACGT]CC
PshAI 82 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 704 CAGCTG CAG^CTG
RcaI 396 TCATGA T^CATGA
RsaI 250 GTAC GT^AC
Sau3AI 239 GATC ^GATC
SfcI 594 CTACAG C^T[GA][CT]AG
SspBI 249 TGTACA T^GTACA
StyI 118 CCTTGG C^C[AT][AT]GG
TaqI 351 TCGA T^CGA
Tsp45I 376 GTGAC ^GT[GC]AC
TspRI 656 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 351 TCGA T^CGA
XcmI 84 CCATCGTCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 238 GGATCC [GA]^GATC[CT]
XmnI 547 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 239 GATCC GGATC(4/5)
238 GGATC
AvaI 925 CCCGAG C^[CT]CG[GA]G
994 CCCGAG
BsaJI 118 CCTTGG C^C[ACGT][ACGT]GG
242 CCACGG
BsmAI 330 GAGAC GTCTC(1/5)
219 GTCTC
BsoBI 925 CCCGAG C^[CT]CG[GA]G
994 CCCGAG
BspMI 864 ACCTGC ACCTGC(4/8)
486 GCAGGT
Cac8I 564 GCAAGC GC[ACGT]^[ACGT]GC
579 GCGGGC
HaeIII 692 GGCC GG^CC
730 GGCC
HphI 375 GGTGA GGTGA(8/7)
608 TCACC
MaeII 462 ACGT A^CGT
558 ACGT
MaeIII 123 GTTAC ^GT[ACGT]AC
376 GTGAC
MseI 296 TTAA T^TAA
715 TTAA
MwoI 564 GCAAGCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
684 GCCCTACTGGC
SfaNI 599 GATGC GCATC(5/9)
663 GCATC
Tru9I 296 TTAA T^TAA
715 TTAA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbsI BbvCI BpuAI
BsaBI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII Fnu4HI FokI ItaI
MamI MboII MnlI MslI MspA1I
MvaI NlaIII Sau96I ScrFI SmlI
Tsp509I