Restriction map for
pFN21AB7383
(Linear) (Six-base) MAPSORT of: KIBB7383.seq Check: 7864 from: 1 to: 1050
KIBB7383.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 873 1050
Size: 477 396 177
Fragments arranged by size:
477 396 177
AccI GT'mk_AC
Cuts at: 0 282 1050
Size: 282 768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 659 1050
Size: 223 436 391
Fragments arranged by size:
436 391 223
ApoI r'AATT_y
Cuts at: 0 157 1050
Size: 157 893
AvaI C'yCGr_G
Cuts at: 0 925 994 1050
Size: 925 69 56
Fragments arranged by size:
925 69 56
BamHI G'GATC_C
Cuts at: 0 238 1050
Size: 238 812
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 317 427 1050
Size: 211 106 110 623
Fragments arranged by size:
623 211 110 106
BbvCI CC'TCA_GC
Cuts at: 0 828 840 897 954 966 1025 1050
Size: 828 12 57 57 12 59 25
Fragments arranged by size:
828 59 57 57 25 12 12
BglI GCCn_nnn'nGGC
Cuts at: 0 690 1050
Size: 690 360
BlpI GC'TnA_GC
Cuts at: 0 28 1050
Size: 28 1022
Bpu10I CC'TnA_GC
Cuts at: 0 12 781 828 840 897 954 966 1025
Size: 12 769 47 12 57 57 12 59
Cuts at: 1025 1050
Size: 25
Fragments arranged by size:
769 59 57 57 47 25 12 12
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 841 898 967 1026 1050
Size: 772 69 57 69 59 24
Fragments arranged by size:
772 69 69 59 57 24
BsaBI GATnn'nnATC
Cuts at: 0 851 908 977 1036 1050
Size: 851 57 69 59 14
Fragments arranged by size:
851 69 59 57 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 734 1050
Size: 734 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 1050
Size: 159 891
BsiHKAI G_wGCw'C
Cuts at: 0 470 1050
Size: 470 580
BsmBI CGTCTCn'nnnn_
Cuts at: 0 224 1050
Size: 224 826
Bsp1286I G_dGCh'C
Cuts at: 0 470 1050
Size: 470 580
BspHI T'CATG_A
Cuts at: 0 396 1050
Size: 396 654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 873 1050
Size: 477 396 177
Fragments arranged by size:
477 396 177
BsrBI CCG'CTC
Cuts at: 0 32 1050
Size: 32 1018
BsrGI T'GTAC_A
Cuts at: 0 249 1050
Size: 249 801
BstAPI GCAn_nnn'nTGC
Cuts at: 0 570 1050
Size: 570 480
BstYI r'GATC_y
Cuts at: 0 238 1050
Size: 238 812
BtgI C'CryG_G
Cuts at: 0 242 1050
Size: 242 808
BtsI GCAGTG_nn'
Cuts at: 0 662 1050
Size: 662 388
DraI TTT'AAA
Cuts at: 0 716 1050
Size: 716 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 659 1050
Size: 223 436 391
Fragments arranged by size:
436 391 223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 1050
Size: 228 822
FspI TGC'GCA
Cuts at: 0 563 1050
Size: 563 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 344 376 590 622 1050
Size: 344 32 214 32 428
Fragments arranged by size:
428 344 214 32 32
HincII GTy'rAC
Cuts at: 0 390 1050
Size: 390 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 772 1050
Size: 296 476 278
Fragments arranged by size:
476 296 278
MslI CAynn'nnrTG
Cuts at: 0 136 541 547 1050
Size: 136 405 6 503
Fragments arranged by size:
503 405 136 6
MspA1I CmG'CkG
Cuts at: 0 706 844 901 970 1029 1050
Size: 706 138 57 69 59 21
Fragments arranged by size:
706 138 69 59 57 21
PfoI T'CCnGG_A
Cuts at: 0 305 1050
Size: 305 745
PshAI GACnn'nnGTC
Cuts at: 0 86 1050
Size: 86 964
PsiI TTA'TAA
Cuts at: 0 155 1050
Size: 155 895
PvuII CAG'CTG
Cuts at: 0 706 1050
Size: 706 344
SfcI C'TryA_G
Cuts at: 0 594 1050
Size: 594 456
SmlI C'TyrA_G
Cuts at: 0 787 856 913 982 1041 1050
Size: 787 69 57 69 59 9
Fragments arranged by size:
787 69 69 59 57 9
StyI C'CwwG_G
Cuts at: 0 118 1050
Size: 118 932
TatI w'GTAC_w
Cuts at: 0 249 1050
Size: 249 801
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 804 836 930 962 1001 1033 1050
Size: 804 32 94 32 39 32 17
Fragments arranged by size:
804 94 39 32 32 32 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 91 1050
Size: 91 959
XmnI GAAnn'nnTTC
Cuts at: 0 551 1050
Size: 551 499
Enzymes that do cut:
AarI AccI AcuI ApoI AvaI BamHI BbsI BbvCI
BglI BlpI Bpu10I BpuEI BsaBI BseRI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BstAPI BstYI
BtgI BtsI DraI Eco57MI FalI FspI Hin4I HincII
MmeI MslI MspA1I PfoI PshAI PsiI PvuII SfcI
SmlI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BanII BbeI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI
BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BspEI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI